82 research outputs found
Development and application of inhibitory luminopsins for the treatment of epilepsy
Optogenetics has shown great promise as a direct neuromodulatory tool for halting seizure activity in various animal models of epilepsy. However, light delivery into the brain is still a major practical challenge that needs to be addressed before future clinical translation is feasible. Not only does light delivery into the brain require surgically implanted hardware that can be both invasive and restrictive, but it is also difficult to illuminate large or complicated structures in the brain due to light scatter and attenuation. We have bypassed the challenges of external light delivery by directly coupling a bioluminescent light source (a genetically encoded Renilla luciferase) to an inhibitory opsin (Natronomonas halorhodopsin) as a single fusion protein, which we term an inhibitory luminopsin (iLMO). iLMOs were developed and characterized in vitro and in vivo using intracellular recordings, multielectrode arrays, and behavioral testing. iLMO2 was shown to generate hyperpolarizing outward currents in response to both external light and luciferase substrate, which was sufficient to suppress action potential firing and synchronous bursting activity in vitro. iLMO2 was further shown to suppress single-unit firing rate and local field potentials in the hippocampus of anesthetized and awake animals. Finally, expression of iLMO was scaled up to multiple structures of the basal ganglia to modulate rotational behavior of freely moving animals in a hardware-independent fashion. iLMO2 was further utilized to acutely suppress focal epileptic discharges induced by intracerebral injection of bicuculline and generalized seizures resulting from systemic administration of pentylenetetrazol. Inhibitory luminopsins have enabled the possibility of optogenetic inhibition of neural activity in a non-invasive and hardware-independent fashion. This work increases the versatility, scalability, and practicality of utilizing optogenetic approaches for halting seizure activity in vivo.Ph.D
QUIS-CAMPI: Biometric Recognition in Surveillance Scenarios
The concerns about individuals security have justified the increasing number of surveillance
cameras deployed both in private and public spaces. However, contrary to popular belief,
these devices are in most cases used solely for recording, instead of feeding intelligent analysis
processes capable of extracting information about the observed individuals. Thus, even though
video surveillance has already proved to be essential for solving multiple crimes, obtaining relevant
details about the subjects that took part in a crime depends on the manual inspection
of recordings. As such, the current goal of the research community is the development of
automated surveillance systems capable of monitoring and identifying subjects in surveillance
scenarios. Accordingly, the main goal of this thesis is to improve the performance of biometric
recognition algorithms in data acquired from surveillance scenarios. In particular, we aim at
designing a visual surveillance system capable of acquiring biometric data at a distance (e.g.,
face, iris or gait) without requiring human intervention in the process, as well as devising biometric
recognition methods robust to the degradation factors resulting from the unconstrained
acquisition process.
Regarding the first goal, the analysis of the data acquired by typical surveillance systems
shows that large acquisition distances significantly decrease the resolution of biometric samples,
and thus their discriminability is not sufficient for recognition purposes. In the literature,
diverse works point out Pan Tilt Zoom (PTZ) cameras as the most practical way for acquiring
high-resolution imagery at a distance, particularly when using a master-slave configuration. In
the master-slave configuration, the video acquired by a typical surveillance camera is analyzed
for obtaining regions of interest (e.g., car, person) and these regions are subsequently imaged
at high-resolution by the PTZ camera. Several methods have already shown that this configuration
can be used for acquiring biometric data at a distance. Nevertheless, these methods
failed at providing effective solutions to the typical challenges of this strategy, restraining its
use in surveillance scenarios. Accordingly, this thesis proposes two methods to support the development
of a biometric data acquisition system based on the cooperation of a PTZ camera
with a typical surveillance camera. The first proposal is a camera calibration method capable
of accurately mapping the coordinates of the master camera to the pan/tilt angles of the PTZ
camera. The second proposal is a camera scheduling method for determining - in real-time -
the sequence of acquisitions that maximizes the number of different targets obtained, while
minimizing the cumulative transition time. In order to achieve the first goal of this thesis,
both methods were combined with state-of-the-art approaches of the human monitoring field
to develop a fully automated surveillance capable of acquiring biometric data at a distance and
without human cooperation, designated as QUIS-CAMPI system.
The QUIS-CAMPI system is the basis for pursuing the second goal of this thesis. The analysis
of the performance of the state-of-the-art biometric recognition approaches shows that these
approaches attain almost ideal recognition rates in unconstrained data. However, this performance
is incongruous with the recognition rates observed in surveillance scenarios. Taking into
account the drawbacks of current biometric datasets, this thesis introduces a novel dataset comprising
biometric samples (face images and gait videos) acquired by the QUIS-CAMPI system at a
distance ranging from 5 to 40 meters and without human intervention in the acquisition process.
This set allows to objectively assess the performance of state-of-the-art biometric recognition
methods in data that truly encompass the covariates of surveillance scenarios. As such, this set
was exploited for promoting the first international challenge on biometric recognition in the wild. This thesis describes the evaluation protocols adopted, along with the results obtained
by the nine methods specially designed for this competition. In addition, the data acquired by
the QUIS-CAMPI system were crucial for accomplishing the second goal of this thesis, i.e., the
development of methods robust to the covariates of surveillance scenarios. The first proposal
regards a method for detecting corrupted features in biometric signatures inferred by a redundancy
analysis algorithm. The second proposal is a caricature-based face recognition approach
capable of enhancing the recognition performance by automatically generating a caricature
from a 2D photo. The experimental evaluation of these methods shows that both approaches
contribute to improve the recognition performance in unconstrained data.A crescente preocupação com a segurança dos indivĂduos tem justificado o crescimento
do nĂşmero de câmaras de vĂdeo-vigilância instaladas tanto em espaços privados como pĂşblicos.
Contudo, ao contrário do que normalmente se pensa, estes dispositivos são, na maior parte dos
casos, usados apenas para gravação, não estando ligados a nenhum tipo de software inteligente
capaz de inferir em tempo real informações sobre os indivĂduos observados. Assim, apesar de a
vĂdeo-vigilância ter provado ser essencial na resolução de diversos crimes, o seu uso está ainda
confinado Ă disponibilização de vĂdeos que tĂŞm que ser manualmente inspecionados para extrair
informações relevantes dos sujeitos envolvidos no crime. Como tal, atualmente, o principal
desafio da comunidade cientĂfica Ă© o desenvolvimento de sistemas automatizados capazes de
monitorizar e identificar indivĂduos em ambientes de vĂdeo-vigilância.
Esta tese tem como principal objetivo estender a aplicabilidade dos sistemas de reconhecimento
biomĂ©trico aos ambientes de vĂdeo-vigilância. De forma mais especifica, pretende-se
1) conceber um sistema de vĂdeo-vigilância que consiga adquirir dados biomĂ©tricos a longas distâncias
(e.g., imagens da cara, Ăris, ou vĂdeos do tipo de passo) sem requerer a cooperação dos
indivĂduos no processo; e 2) desenvolver mĂ©todos de reconhecimento biomĂ©trico robustos aos
fatores de degradação inerentes aos dados adquiridos por este tipo de sistemas.
No que diz respeito ao primeiro objetivo, a análise aos dados adquiridos pelos sistemas tĂpicos
de vĂdeo-vigilância mostra que, devido Ă distância de captura, os traços biomĂ©tricos amostrados
não são suficientemente discriminativos para garantir taxas de reconhecimento aceitáveis.
Na literatura, vários trabalhos advogam o uso de câmaras Pan Tilt Zoom (PTZ) para adquirir
imagens de alta resolução à distância, principalmente o uso destes dispositivos no modo masterslave.
Na configuração master-slave um módulo de análise inteligente seleciona zonas de interesse
(e.g. carros, pessoas) a partir do vĂdeo adquirido por uma câmara de vĂdeo-vigilância
e a câmara PTZ é orientada para adquirir em alta resolução as regiões de interesse. Diversos
métodos já mostraram que esta configuração pode ser usada para adquirir dados biométricos
à distância, ainda assim estes não foram capazes de solucionar alguns problemas relacionados
com esta estratĂ©gia, impedindo assim o seu uso em ambientes de vĂdeo-vigilância. Deste modo,
esta tese propõe dois métodos para permitir a aquisição de dados biométricos em ambientes de
vĂdeo-vigilância usando uma câmara PTZ assistida por uma câmara tĂpica de vĂdeo-vigilância. O
primeiro é um método de calibração capaz de mapear de forma exata as coordenadas da câmara
master para o ângulo da câmara PTZ (slave) sem o auxĂlio de outros dispositivos Ăłticos. O
segundo método determina a ordem pela qual um conjunto de sujeitos vai ser observado pela
câmara PTZ. O método proposto consegue determinar em tempo-real a sequência de observações
que maximiza o nĂşmero de diferentes sujeitos observados e simultaneamente minimiza o
tempo total de transição entre sujeitos. De modo a atingir o primeiro objetivo desta tese, os
dois métodos propostos foram combinados com os avanços alcançados na área da monitorização
de humanos para assim desenvolver o primeiro sistema de vĂdeo-vigilância completamente automatizado
e capaz de adquirir dados biométricos a longas distâncias sem requerer a cooperação
dos indivĂduos no processo, designado por sistema QUIS-CAMPI.
O sistema QUIS-CAMPI representa o ponto de partida para iniciar a investigação relacionada
com o segundo objetivo desta tese. A análise do desempenho dos métodos de reconhecimento
biométrico do estado-da-arte mostra que estes conseguem obter taxas de reconhecimento
quase perfeitas em dados adquiridos sem restrições (e.g., taxas de reconhecimento
maiores do que 99% no conjunto de dados LFW). Contudo, este desempenho nĂŁo Ă© corroborado pelos resultados observados em ambientes de vĂdeo-vigilância, o que sugere que os conjuntos
de dados atuais nĂŁo contĂŞm verdadeiramente os fatores de degradação tĂpicos dos ambientes de
vĂdeo-vigilância. Tendo em conta as vulnerabilidades dos conjuntos de dados biomĂ©tricos atuais,
esta tese introduz um novo conjunto de dados biomĂ©tricos (imagens da face e vĂdeos do tipo de
passo) adquiridos pelo sistema QUIS-CAMPI a uma distância máxima de 40m e sem a cooperação
dos sujeitos no processo de aquisição. Este conjunto permite avaliar de forma objetiva o desempenho
dos mĂ©todos do estado-da-arte no reconhecimento de indivĂduos em imagens/vĂdeos
capturados num ambiente real de vĂdeo-vigilância. Como tal, este conjunto foi utilizado para
promover a primeira competição de reconhecimento biométrico em ambientes não controlados.
Esta tese descreve os protocolos de avaliação usados, assim como os resultados obtidos por 9
métodos especialmente desenhados para esta competição. Para além disso, os dados adquiridos
pelo sistema QUIS-CAMPI foram essenciais para o desenvolvimento de dois métodos para
aumentar a robustez aos fatores de degradação observados em ambientes de vĂdeo-vigilância. O
primeiro Ă© um mĂ©todo para detetar caracterĂsticas corruptas em assinaturas biomĂ©tricas atravĂ©s
da análise da redundância entre subconjuntos de caracterĂsticas. O segundo Ă© um mĂ©todo de
reconhecimento facial baseado em caricaturas automaticamente geradas a partir de uma Ăşnica
foto do sujeito. As experiências realizadas mostram que ambos os métodos conseguem reduzir
as taxas de erro em dados adquiridos de forma nĂŁo controlada
Video object tracking : contributions to object description and performance assessment
Tese de doutoramento. Engenharia Electrotécnica e de Computadores. Universidade do Porto. Faculdade de Engenharia. 201
Occlusion handling in multiple people tracking
Object tracking with occlusion handling is a challenging problem in automated video surveillance. Occlusion handling and tracking have always been considered as separate modules. We have proposed an automated video surveillance system, which automatically detects occlusions and perform occlusion handling, while the tracker continues to track resulting separated objects. A new approach based on sub-blobbing is presented for tracking objects accurately and steadily, when the target encounters occlusion in video sequences. We have used a feature-based framework for tracking, which involves feature extraction and feature matching
Survey on video anomaly detection in dynamic scenes with moving cameras
The increasing popularity of compact and inexpensive cameras, e.g.~dash
cameras, body cameras, and cameras equipped on robots, has sparked a growing
interest in detecting anomalies within dynamic scenes recorded by moving
cameras. However, existing reviews primarily concentrate on Video Anomaly
Detection (VAD) methods assuming static cameras. The VAD literature with moving
cameras remains fragmented, lacking comprehensive reviews to date. To address
this gap, we endeavor to present the first comprehensive survey on Moving
Camera Video Anomaly Detection (MC-VAD). We delve into the research papers
related to MC-VAD, critically assessing their limitations and highlighting
associated challenges. Our exploration encompasses three application domains:
security, urban transportation, and marine environments, which in turn cover
six specific tasks. We compile an extensive list of 25 publicly-available
datasets spanning four distinct environments: underwater, water surface,
ground, and aerial. We summarize the types of anomalies these datasets
correspond to or contain, and present five main categories of approaches for
detecting such anomalies. Lastly, we identify future research directions and
discuss novel contributions that could advance the field of MC-VAD. With this
survey, we aim to offer a valuable reference for researchers and practitioners
striving to develop and advance state-of-the-art MC-VAD methods.Comment: Under revie
3D Pedestrian Tracking and Virtual Reconstruction of Ceramic Vessels Using Geometric and Color Cues
Object tracking using cameras has many applications ranging from monitoring children and the elderly, to behavior analysis, entertainment, and homeland security. This thesis concentrates on the problem of tracking person(s) of interest in crowded scenes (e.g., airports, train stations, malls, etc.), rendering their locations in time and space along with high quality close-up images of the person for recognition. The tracking is achieved using a combination of overhead cameras for 3D tracking and a network of pan-tilt-zoom (PTZ) cameras to obtain close-up frontal face images. Based on projective geometry, the overhead cameras track people using salient and easily computable feature points such as head points. When the obtained head point is not accurate enough, the color information of the head tops across subsequent frames is integrated to detect and track people. To capture the best frontal face images of a target across time, a PTZ camera scheduling is proposed, where the 'best' PTZ camera is selected based on the capture quality (as close as possible to frontal view) and handoff success (response time needed by the newly selected camera to move from current to desired state) probabilities. The experiments show the 3D tracking errors are very small (less than 5 cm with 14 people crowding an area of around 4 m2) and the frontal face images are captured effectively with most of them centering in the frames. Computational archaeology is becoming a success story of applying computational tools in the reconstruction of vessels obtained from digs, freeing the expert from hours of intensive labor in manually stitching shards into meaningful vessels. In this thesis, we concentrate on the use of geometric and color information of the fragments for 3D virtual reconstruction of broken ceramic vessels. Generic models generated by the experts as a rendition of what the original vessel may have looked like are also utilized. The generic models need not to be identical to the original vessel, but are within a geometric transformation of it in most of its parts. The markings on the 3D surfaces of fragments and generic models are extracted based on their color cues. Ceramic fragments are then aligned against the corresponding generic models based on the geometric relation between the extracted markings. The alignments yield sub-scanner resolution fitting errors.Ph.D., Electrical Engineering -- Drexel University, 201
Biometric fusion methods for adaptive face recognition in computer vision
PhD ThesisFace recognition is a biometric method that uses different techniques to identify the individuals based on the facial information received from digital image data. The system of face recognition is widely used for security purposes, which has challenging problems. The solutions to some of the most important challenges are proposed in this study. The aim of this thesis is to investigate face recognition across pose problem based on the image parameters of camera calibration. In this thesis, three novel methods have been derived to address the challenges of face recognition and offer solutions to infer the camera parameters from images using a geomtric approach based on perspective projection. The following techniques were used: camera calibration CMT and Face Quadtree Decomposition (FQD), in order to develop the face camera measurement technique (FCMT) for human facial recognition.
Facial information from a feature extraction and identity-matching algorithm has been created. The success and efficacy of the proposed algorithm are analysed in terms of robustness to noise, the accuracy of distance measurement, and face recognition. To overcome the intrinsic and extrinsic parameters of camera calibration parameters, a novel technique has been developed based on perspective projection, which uses different geometrical shapes to calibrate the camera. The parameters used in novel measurement technique CMT that enables the system to infer the real distance for regular and irregular objects from the 2-D images. The proposed system of CMT feeds into FQD to measure the distance between the facial points. Quadtree decomposition enhances the representation of edges and other singularities along curves of the face, and thus improves directional features from face detection across face pose. The proposed FCMT system is the new combination of CMT and FQD to recognise the faces in the various pose.
The theoretical foundation of the proposed solutions has been thoroughly developed and discussed in detail. The results show that the proposed algorithms outperform existing algorithms in face recognition, with a 2.5% improvement in main error recognition rate compared with recent studies
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