82 research outputs found

    Development and application of inhibitory luminopsins for the treatment of epilepsy

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    Optogenetics has shown great promise as a direct neuromodulatory tool for halting seizure activity in various animal models of epilepsy. However, light delivery into the brain is still a major practical challenge that needs to be addressed before future clinical translation is feasible. Not only does light delivery into the brain require surgically implanted hardware that can be both invasive and restrictive, but it is also difficult to illuminate large or complicated structures in the brain due to light scatter and attenuation. We have bypassed the challenges of external light delivery by directly coupling a bioluminescent light source (a genetically encoded Renilla luciferase) to an inhibitory opsin (Natronomonas halorhodopsin) as a single fusion protein, which we term an inhibitory luminopsin (iLMO). iLMOs were developed and characterized in vitro and in vivo using intracellular recordings, multielectrode arrays, and behavioral testing. iLMO2 was shown to generate hyperpolarizing outward currents in response to both external light and luciferase substrate, which was sufficient to suppress action potential firing and synchronous bursting activity in vitro. iLMO2 was further shown to suppress single-unit firing rate and local field potentials in the hippocampus of anesthetized and awake animals. Finally, expression of iLMO was scaled up to multiple structures of the basal ganglia to modulate rotational behavior of freely moving animals in a hardware-independent fashion. iLMO2 was further utilized to acutely suppress focal epileptic discharges induced by intracerebral injection of bicuculline and generalized seizures resulting from systemic administration of pentylenetetrazol. Inhibitory luminopsins have enabled the possibility of optogenetic inhibition of neural activity in a non-invasive and hardware-independent fashion. This work increases the versatility, scalability, and practicality of utilizing optogenetic approaches for halting seizure activity in vivo.Ph.D

    QUIS-CAMPI: Biometric Recognition in Surveillance Scenarios

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    The concerns about individuals security have justified the increasing number of surveillance cameras deployed both in private and public spaces. However, contrary to popular belief, these devices are in most cases used solely for recording, instead of feeding intelligent analysis processes capable of extracting information about the observed individuals. Thus, even though video surveillance has already proved to be essential for solving multiple crimes, obtaining relevant details about the subjects that took part in a crime depends on the manual inspection of recordings. As such, the current goal of the research community is the development of automated surveillance systems capable of monitoring and identifying subjects in surveillance scenarios. Accordingly, the main goal of this thesis is to improve the performance of biometric recognition algorithms in data acquired from surveillance scenarios. In particular, we aim at designing a visual surveillance system capable of acquiring biometric data at a distance (e.g., face, iris or gait) without requiring human intervention in the process, as well as devising biometric recognition methods robust to the degradation factors resulting from the unconstrained acquisition process. Regarding the first goal, the analysis of the data acquired by typical surveillance systems shows that large acquisition distances significantly decrease the resolution of biometric samples, and thus their discriminability is not sufficient for recognition purposes. In the literature, diverse works point out Pan Tilt Zoom (PTZ) cameras as the most practical way for acquiring high-resolution imagery at a distance, particularly when using a master-slave configuration. In the master-slave configuration, the video acquired by a typical surveillance camera is analyzed for obtaining regions of interest (e.g., car, person) and these regions are subsequently imaged at high-resolution by the PTZ camera. Several methods have already shown that this configuration can be used for acquiring biometric data at a distance. Nevertheless, these methods failed at providing effective solutions to the typical challenges of this strategy, restraining its use in surveillance scenarios. Accordingly, this thesis proposes two methods to support the development of a biometric data acquisition system based on the cooperation of a PTZ camera with a typical surveillance camera. The first proposal is a camera calibration method capable of accurately mapping the coordinates of the master camera to the pan/tilt angles of the PTZ camera. The second proposal is a camera scheduling method for determining - in real-time - the sequence of acquisitions that maximizes the number of different targets obtained, while minimizing the cumulative transition time. In order to achieve the first goal of this thesis, both methods were combined with state-of-the-art approaches of the human monitoring field to develop a fully automated surveillance capable of acquiring biometric data at a distance and without human cooperation, designated as QUIS-CAMPI system. The QUIS-CAMPI system is the basis for pursuing the second goal of this thesis. The analysis of the performance of the state-of-the-art biometric recognition approaches shows that these approaches attain almost ideal recognition rates in unconstrained data. However, this performance is incongruous with the recognition rates observed in surveillance scenarios. Taking into account the drawbacks of current biometric datasets, this thesis introduces a novel dataset comprising biometric samples (face images and gait videos) acquired by the QUIS-CAMPI system at a distance ranging from 5 to 40 meters and without human intervention in the acquisition process. This set allows to objectively assess the performance of state-of-the-art biometric recognition methods in data that truly encompass the covariates of surveillance scenarios. As such, this set was exploited for promoting the first international challenge on biometric recognition in the wild. This thesis describes the evaluation protocols adopted, along with the results obtained by the nine methods specially designed for this competition. In addition, the data acquired by the QUIS-CAMPI system were crucial for accomplishing the second goal of this thesis, i.e., the development of methods robust to the covariates of surveillance scenarios. The first proposal regards a method for detecting corrupted features in biometric signatures inferred by a redundancy analysis algorithm. The second proposal is a caricature-based face recognition approach capable of enhancing the recognition performance by automatically generating a caricature from a 2D photo. The experimental evaluation of these methods shows that both approaches contribute to improve the recognition performance in unconstrained data.A crescente preocupação com a segurança dos indivíduos tem justificado o crescimento do número de câmaras de vídeo-vigilância instaladas tanto em espaços privados como públicos. Contudo, ao contrário do que normalmente se pensa, estes dispositivos são, na maior parte dos casos, usados apenas para gravação, não estando ligados a nenhum tipo de software inteligente capaz de inferir em tempo real informações sobre os indivíduos observados. Assim, apesar de a vídeo-vigilância ter provado ser essencial na resolução de diversos crimes, o seu uso está ainda confinado à disponibilização de vídeos que têm que ser manualmente inspecionados para extrair informações relevantes dos sujeitos envolvidos no crime. Como tal, atualmente, o principal desafio da comunidade científica é o desenvolvimento de sistemas automatizados capazes de monitorizar e identificar indivíduos em ambientes de vídeo-vigilância. Esta tese tem como principal objetivo estender a aplicabilidade dos sistemas de reconhecimento biométrico aos ambientes de vídeo-vigilância. De forma mais especifica, pretende-se 1) conceber um sistema de vídeo-vigilância que consiga adquirir dados biométricos a longas distâncias (e.g., imagens da cara, íris, ou vídeos do tipo de passo) sem requerer a cooperação dos indivíduos no processo; e 2) desenvolver métodos de reconhecimento biométrico robustos aos fatores de degradação inerentes aos dados adquiridos por este tipo de sistemas. No que diz respeito ao primeiro objetivo, a análise aos dados adquiridos pelos sistemas típicos de vídeo-vigilância mostra que, devido à distância de captura, os traços biométricos amostrados não são suficientemente discriminativos para garantir taxas de reconhecimento aceitáveis. Na literatura, vários trabalhos advogam o uso de câmaras Pan Tilt Zoom (PTZ) para adquirir imagens de alta resolução à distância, principalmente o uso destes dispositivos no modo masterslave. Na configuração master-slave um módulo de análise inteligente seleciona zonas de interesse (e.g. carros, pessoas) a partir do vídeo adquirido por uma câmara de vídeo-vigilância e a câmara PTZ é orientada para adquirir em alta resolução as regiões de interesse. Diversos métodos já mostraram que esta configuração pode ser usada para adquirir dados biométricos à distância, ainda assim estes não foram capazes de solucionar alguns problemas relacionados com esta estratégia, impedindo assim o seu uso em ambientes de vídeo-vigilância. Deste modo, esta tese propõe dois métodos para permitir a aquisição de dados biométricos em ambientes de vídeo-vigilância usando uma câmara PTZ assistida por uma câmara típica de vídeo-vigilância. O primeiro é um método de calibração capaz de mapear de forma exata as coordenadas da câmara master para o ângulo da câmara PTZ (slave) sem o auxílio de outros dispositivos óticos. O segundo método determina a ordem pela qual um conjunto de sujeitos vai ser observado pela câmara PTZ. O método proposto consegue determinar em tempo-real a sequência de observações que maximiza o número de diferentes sujeitos observados e simultaneamente minimiza o tempo total de transição entre sujeitos. De modo a atingir o primeiro objetivo desta tese, os dois métodos propostos foram combinados com os avanços alcançados na área da monitorização de humanos para assim desenvolver o primeiro sistema de vídeo-vigilância completamente automatizado e capaz de adquirir dados biométricos a longas distâncias sem requerer a cooperação dos indivíduos no processo, designado por sistema QUIS-CAMPI. O sistema QUIS-CAMPI representa o ponto de partida para iniciar a investigação relacionada com o segundo objetivo desta tese. A análise do desempenho dos métodos de reconhecimento biométrico do estado-da-arte mostra que estes conseguem obter taxas de reconhecimento quase perfeitas em dados adquiridos sem restrições (e.g., taxas de reconhecimento maiores do que 99% no conjunto de dados LFW). Contudo, este desempenho não é corroborado pelos resultados observados em ambientes de vídeo-vigilância, o que sugere que os conjuntos de dados atuais não contêm verdadeiramente os fatores de degradação típicos dos ambientes de vídeo-vigilância. Tendo em conta as vulnerabilidades dos conjuntos de dados biométricos atuais, esta tese introduz um novo conjunto de dados biométricos (imagens da face e vídeos do tipo de passo) adquiridos pelo sistema QUIS-CAMPI a uma distância máxima de 40m e sem a cooperação dos sujeitos no processo de aquisição. Este conjunto permite avaliar de forma objetiva o desempenho dos métodos do estado-da-arte no reconhecimento de indivíduos em imagens/vídeos capturados num ambiente real de vídeo-vigilância. Como tal, este conjunto foi utilizado para promover a primeira competição de reconhecimento biométrico em ambientes não controlados. Esta tese descreve os protocolos de avaliação usados, assim como os resultados obtidos por 9 métodos especialmente desenhados para esta competição. Para além disso, os dados adquiridos pelo sistema QUIS-CAMPI foram essenciais para o desenvolvimento de dois métodos para aumentar a robustez aos fatores de degradação observados em ambientes de vídeo-vigilância. O primeiro é um método para detetar características corruptas em assinaturas biométricas através da análise da redundância entre subconjuntos de características. O segundo é um método de reconhecimento facial baseado em caricaturas automaticamente geradas a partir de uma única foto do sujeito. As experiências realizadas mostram que ambos os métodos conseguem reduzir as taxas de erro em dados adquiridos de forma não controlada

    Video object tracking : contributions to object description and performance assessment

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    Tese de doutoramento. Engenharia Electrotécnica e de Computadores. Universidade do Porto. Faculdade de Engenharia. 201

    Occlusion handling in multiple people tracking

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    Object tracking with occlusion handling is a challenging problem in automated video surveillance. Occlusion handling and tracking have always been considered as separate modules. We have proposed an automated video surveillance system, which automatically detects occlusions and perform occlusion handling, while the tracker continues to track resulting separated objects. A new approach based on sub-blobbing is presented for tracking objects accurately and steadily, when the target encounters occlusion in video sequences. We have used a feature-based framework for tracking, which involves feature extraction and feature matching

    Survey on video anomaly detection in dynamic scenes with moving cameras

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    The increasing popularity of compact and inexpensive cameras, e.g.~dash cameras, body cameras, and cameras equipped on robots, has sparked a growing interest in detecting anomalies within dynamic scenes recorded by moving cameras. However, existing reviews primarily concentrate on Video Anomaly Detection (VAD) methods assuming static cameras. The VAD literature with moving cameras remains fragmented, lacking comprehensive reviews to date. To address this gap, we endeavor to present the first comprehensive survey on Moving Camera Video Anomaly Detection (MC-VAD). We delve into the research papers related to MC-VAD, critically assessing their limitations and highlighting associated challenges. Our exploration encompasses three application domains: security, urban transportation, and marine environments, which in turn cover six specific tasks. We compile an extensive list of 25 publicly-available datasets spanning four distinct environments: underwater, water surface, ground, and aerial. We summarize the types of anomalies these datasets correspond to or contain, and present five main categories of approaches for detecting such anomalies. Lastly, we identify future research directions and discuss novel contributions that could advance the field of MC-VAD. With this survey, we aim to offer a valuable reference for researchers and practitioners striving to develop and advance state-of-the-art MC-VAD methods.Comment: Under revie

    3D Pedestrian Tracking and Virtual Reconstruction of Ceramic Vessels Using Geometric and Color Cues

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    Object tracking using cameras has many applications ranging from monitoring children and the elderly, to behavior analysis, entertainment, and homeland security. This thesis concentrates on the problem of tracking person(s) of interest in crowded scenes (e.g., airports, train stations, malls, etc.), rendering their locations in time and space along with high quality close-up images of the person for recognition. The tracking is achieved using a combination of overhead cameras for 3D tracking and a network of pan-tilt-zoom (PTZ) cameras to obtain close-up frontal face images. Based on projective geometry, the overhead cameras track people using salient and easily computable feature points such as head points. When the obtained head point is not accurate enough, the color information of the head tops across subsequent frames is integrated to detect and track people. To capture the best frontal face images of a target across time, a PTZ camera scheduling is proposed, where the 'best' PTZ camera is selected based on the capture quality (as close as possible to frontal view) and handoff success (response time needed by the newly selected camera to move from current to desired state) probabilities. The experiments show the 3D tracking errors are very small (less than 5 cm with 14 people crowding an area of around 4 m2) and the frontal face images are captured effectively with most of them centering in the frames. Computational archaeology is becoming a success story of applying computational tools in the reconstruction of vessels obtained from digs, freeing the expert from hours of intensive labor in manually stitching shards into meaningful vessels. In this thesis, we concentrate on the use of geometric and color information of the fragments for 3D virtual reconstruction of broken ceramic vessels. Generic models generated by the experts as a rendition of what the original vessel may have looked like are also utilized. The generic models need not to be identical to the original vessel, but are within a geometric transformation of it in most of its parts. The markings on the 3D surfaces of fragments and generic models are extracted based on their color cues. Ceramic fragments are then aligned against the corresponding generic models based on the geometric relation between the extracted markings. The alignments yield sub-scanner resolution fitting errors.Ph.D., Electrical Engineering -- Drexel University, 201

    Biometric fusion methods for adaptive face recognition in computer vision

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    PhD ThesisFace recognition is a biometric method that uses different techniques to identify the individuals based on the facial information received from digital image data. The system of face recognition is widely used for security purposes, which has challenging problems. The solutions to some of the most important challenges are proposed in this study. The aim of this thesis is to investigate face recognition across pose problem based on the image parameters of camera calibration. In this thesis, three novel methods have been derived to address the challenges of face recognition and offer solutions to infer the camera parameters from images using a geomtric approach based on perspective projection. The following techniques were used: camera calibration CMT and Face Quadtree Decomposition (FQD), in order to develop the face camera measurement technique (FCMT) for human facial recognition. Facial information from a feature extraction and identity-matching algorithm has been created. The success and efficacy of the proposed algorithm are analysed in terms of robustness to noise, the accuracy of distance measurement, and face recognition. To overcome the intrinsic and extrinsic parameters of camera calibration parameters, a novel technique has been developed based on perspective projection, which uses different geometrical shapes to calibrate the camera. The parameters used in novel measurement technique CMT that enables the system to infer the real distance for regular and irregular objects from the 2-D images. The proposed system of CMT feeds into FQD to measure the distance between the facial points. Quadtree decomposition enhances the representation of edges and other singularities along curves of the face, and thus improves directional features from face detection across face pose. The proposed FCMT system is the new combination of CMT and FQD to recognise the faces in the various pose. The theoretical foundation of the proposed solutions has been thoroughly developed and discussed in detail. The results show that the proposed algorithms outperform existing algorithms in face recognition, with a 2.5% improvement in main error recognition rate compared with recent studies

    Visual analysis of faces with application in biometrics, forensics and health informatics

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