Molecular identification of causative CL species.

Abstract

<p>Restriction fragment length polymorphism (RFLP) analysis of the amplified internal transcribed spacer 1 region (ITS1) digested with restriction enzyme <i>Hae</i>III and analysed by electrophoresis on 2.5% agarose gels. Three reference strains were used for comparison; Lane 1 = <i>L. major</i>: MHOM/PS/01/ISL659, Lane 2 = <i>L. tropica</i>: MHOM/PS/02/63JnF21 and Lane 3 = <i>L. infantum</i> MHOM/TN/1980/IPT1. 1 kb = molecular size marker. All other lanes show digested PCR product from clinical materials; lanes 4–7 = <i>L. tropica</i> cases from Al Jabal Al Gharbi, Misrata and Tarhuna districts; lanes 8–11 = <i>L.major</i> cases from Tripoli, Sirt, Misrata, Al Murqub.</p