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    Influential cited references in FEMS Microbiology Letters: lessons from Reference Publication Year Spectroscopy (RPYS)

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    The journal FEMS Microbiology Letters covers all aspects of microbiology including virology. On which scientific shoulders do the papers published in this journal stand? Which are the classic papers used by the authors? We aim to answer these questions in this study by applying the Reference Publication Year Spectroscopy (RPYS) analysis to all papers published in this journal between 1977 and 2017. In total, 16 837 publications with 410 586 cited references are analyzed. Mainly, the studies published in the journal FEMS Microbiology Letters draw knowledge from methods developed to quantify or characterize biochemical substances such as proteins, nucleic acids, lipids, or carbohydrates and from improvements of techniques suitable for studies of bacterial genetics. The techniques frequently used for studying the genetic of microorganisms in FEMS Microbiology Letters' studies were developed using samples prepared from microorganisms. Methods required for the investigation of proteins, carbohydrates, or lipids were mostly transferred from other fields of life science to microbiology

    MICROBIOLOGY OF SOIL

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    The discovery that certain microorganisms isolated from soil could produce antibiotics when grown in pure cultures in the lab­ oratory has served to focus attention as never before on the sub­ject of soil microbiology. The main emphasis, however, is on prob­lems entirely unrelated to the processes which go on in the soil. The fact that an organism was isolated from soil and then used in medicine, fermentations, or some such way, does not bring such work in the actual field of soil microbiology. Practically all except the more fastidious animal and plant pathogens can be found in the soil at one time or another. Even some of the fastidious or­ ganisms are quite regularly found. That is not surprising consider­ ing that all sorts of materials find their way back to the soil-the dumping place of much of our refuse. In addition, the soil serves as a good matrix for the preservation of microorganisms, provided competition between the various forms is not too severe. Waksman (1) reviewed the literature up to 1932, and then supplemented his book with a review (2) covering the years 1932- 36. A decade later, Norman (3) very briefly surveyed the field and discussed the status of the science, especially the lack of support allotted to it. It is the purpose of the present paper to review some of the more important work published since 1936. The coverage of the literature cannot be complete owing to the limitation of space; more attention will be paid to some work, less to others. Responsibility for the selection of the references discussed will be the reviewer\u27s, who will be influenced a great deal by his own in­terests

    Absent in vitro interaction between chloroquine and antifungals against Aspergillus fumigatus

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    This work was supported by Medical Mycology Section, Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, the Netherlands. Parts of these results were presented at the ASM Microbe Conference of American Society for Microbiology, June 16–20, 2016, Boston, MA, Poster no. 426,Peer reviewedPostprin

    Laboratory Focus on Improving the Culture of Biosafety: Statewide Risk Assessment of Clinical Laboratories That Process Specimens for Microbiologic Analysis

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    The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source and test requisition. Over 88% of the respondents complied with more than three-quarters of the mitigation control measures listed in the survey. Facility assessment revealed that subsets of laboratories that claim biosafety level 1, 2, or 3 status did not possess all of the biosafety elements considered minimally standard for their respective classifications. Many laboratories reported being able to quickly correct the minor deficiencies identified. Task assessment identified deficiencies that trended higher within the general (not microbiology-specific) laboratory for core activities, such as packaging and shipping, direct microscopic examination, and culture modalities solely involving screens for organism growth. For traditional microbiology departments, opportunities for improvement in the cultivation and management of highly infectious agents, such as acid-fast bacilli and systemic fungi, were revealed. These results derived from a survey of a large cohort of small- and large-scale laboratories suggest the necessity for continued microbiology-based understanding of biosafety practices, vigilance toward biosafety, and enforcement of biosafety practices throughout the laboratory setting

    Microbial Effects on Repository Performance

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    This report presents a critical review of the international literature on microbial effects in and around a deep geological repository for higher activity wastes. It is aimed at those who are familiar with the nuclear industry and radioactive waste disposal, but who are not experts in microbiology; they may have a limited knowledge of how microbiology may be integrated into and impact upon radioactive waste disposal safety cases and associated performance assessments (PA)

    Robert Koch, Creation, and the Specificity of Germs

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    Microbiology is dominated by evolution today. Just look at any text, journal article, or the topics presented at professional scientific meetings. Darwin is dominant. Microbiology is dominated by evolution today. Just look at any text, journal article, or the topics presented at professional scientific meetings. Darwin is dominant. Many argue that “nothing in biology makes sense except in the light of evolution” (Dobzhansky 1973). But it was not always this way. In fact, a review of the major founders of microbiology has shown that they were creationists.1 We would argue that a better idea thanevolution and one of much more practical importance is the germ theory of disease, originally put forth primarily by non-Darwinian biologists (Gillen and Oliver 2009). In our previous article (Gillen and Oliver 2009), we documented these and many other creation and Christian contributions to germ theory. But only recently has it become known that another important microbiology founder, Robert Koch (Fig. 1) and his co-workers were Linnaean creationists in their classification.2 This is due, in part, to additional works of Robert Koch that were translated from German to English. The year 2010 marks the 100thanniversary of his death (died: May 27, 1910). Although Koch and other German microbiologists were fairly secular in their thinking, their acceptance of Darwinian evolution was minimal
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