165,871 research outputs found

    Revalorizando el bosque seco de algarrobo : estudio y an谩lisis de la biodiversidad, distribuci贸n y conservaci贸n de los bosques secos en Lambayeque

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    En nuestro planeta, gran parte del 谩rea continental ha estado cubierta de bosques por millones de a帽os. En el Per煤, tanto la costa, la sierra como la selva poseen grandes bosques, que en conjunto ocupan el 51% del territorio. Los bosques secos son ecosistemas caracter铆sticos de la costa norte peruana, siendo su especie clave el algarrobo (Prosopis pallida), la cual que no solo representa un valor ecol贸gico sino tambi茅n econ贸mico para las comunidades aleda帽as a dichos ecosistemas. Esta investigaci贸n se centra en los bosques de Lambayeque, siendo definidos como representaciones de bosques secos de algarrobo los que se ubican en el Santuario Hist贸rico Bosque de P贸mac, el ACP Chaparr铆 y el ACR Huacrupe La Calera. Actualmente, es evidente que los procesos de deforestaci贸n son las causas m谩s fuertes de la reducci贸n de muchas especies forestales. Esto en conjunto con los bruscos cambios de uso de suelo, los incendios forestales de origen antr贸pico y el cambio clim谩tico vienen afectando a las comunidades humanas y muchas poblaciones de especies animales y vegetales. Una de las especies que se ve amenazada por estos factores es, precisamente, el algarrobo, por lo que su conservaci贸n junto al ecosistema de bosque seco en su plenitud es prioritaria. Por tanto, el objetivo de esta tesis es identificar y plantear qu茅 estrategias son las m谩s adecuadas para conservar y gestionar los bosques secos de manera integrada, de manera que se valoricen los recursos y servicios que ofrecen estos ecosistemas. Se trabaj贸 una metodolog铆a basada en biogeograf铆a de la conservaci贸n, la cual incluy贸 m茅todos de modelamiento de distribuci贸n de especies (MDE), sensores remotos y sistemas de informaci贸n geogr谩fica (SIG). Se utiliz贸 el software Maxent, un programa de modelamiento basado en el principio de m谩xima entrop铆a, para obtener la distribuci贸n potencial presente del algarrobo, as铆 como la distribuci贸n potencial futura (para el a帽o 2050) bajo dos escenarios RCP 2,6 y 8,5, resultando en un inesperado aumento en la potencialidad del norte peruano para albergar la especie. Para conocer la din谩mica de los bosques secos en cuanto a cambios en cobertura vegetal, frecuencia de incendios y fragmentaci贸n del paisaje se utilizaron herramientas SIG y de teledetecci贸n (como el NDVI) para realizar un an谩lisis espacial y multitemporal, dando como resultado variaciones en la cobertura vegetal, pero que tienden a la reducci贸n de la vegetaci贸n hacia los 煤ltimos a帽os. Adem谩s, se evidencia una ampliaci贸n de la frontera agr铆cola debido principalmente a la deforestaci贸n. Por otra parte, no se puede omitir el rol de los eventos del ENSO en una zona como esta, pues sus efectos influyen en la regeneraci贸n de sus bosques. El trabajo de campo consisti贸 en dos partes esenciales: en primer lugar, obtener el 铆ndice de valor de importancia (IVI) del algarrobo en el bosque seco. El m茅todo de muestreo realizado fue el punto 鈥 centro 鈥 cuadrado, obteniendo un valor de 53,01 para el algarrobo, adem谩s de conocer la composici贸n flor铆stica del bosque, tomando como referencia principal el ACR Huacrupe. En segundo lugar, se aplicaron encuestas y entrevistas a actores clave, guardaparques y a la poblaci贸n local, quienes confirmaron la importancia de la especie para el mantenimiento de los bosques secos y como aporte y hasta sustento econ贸mico de diversas comunidades, as铆 como la preocupante situaci贸n de la deforestaci贸n, principalmente ilegal para fines comerciales, degradaci贸n y continua reducci贸n de los 谩rboles de algarrobo.On our planet, much of the continental area has been forested for millions of years. In Peru, the coast, highlands and jungle, have large forests, which together occupy 51% of the territory. Dry forests are characteristic ecosystems of the northern coast of Peru, and its key species Prosopis pallida, which represents not only an ecological but also economic value to communities surrounding these ecosystems. This research focuses on the forests of Lambayeque, being defined as representations of dry forests of carob tree those located in the Pomac Forest Historical Sanctuary, the ACP Chaparr铆 and ACR Huacrupe La Calera. Currently, it is clear that deforestation is the strongest cause of the reduction of many forest species. This coupled with the rapid changes in land use, forest fires and climate change are affecting many human communities and populations of animals and plants. One species that is threatened by these factors is, precisely, carob tree, so conservation along the dry forest ecosystem in its fullness is a priority. Therefore, the objective of this thesis is to identify and propose what strategies are most appropriate to conserve and manage the dry forests in an integrated manner so that resources and services offered by these ecosystems could be valorized. A methodology based on conservation biogeography, which included methods of species distribution modeling (MDE), remote sensing and geographic information systems (GIS), was worked. The Maxent software, a modeling program based on the principle of maximum entropy, for the present potential of the carob tree distribution, and its potential future distribution (2050) under two scenarios RCP 2,6 and 8,5 was used, resulting in an unexpected increase in the potential of northern Peru to host the species. To understand the dynamics of dry forests in terms of changes in land cover, fire frequency and landscape fragmentation, GIS tools and remote sensing methods (such as NDVI) were used to perform a spatial and multi-temporal analysis, resulting in variations in coverage plant, but they tend to reduce the vegetation to recent years. Furthermore, there is a clear expansion of the agricultural frontier, mainly due to deforestation. Moreover, one cannot ignore the role of ENSO events in an area like this, since their effects influence the regeneration of forests. The field work consisted of two main parts. In first place, to obtain the importance value index (IVI) of the carob tree dry forest. The sampling method was the point 鈥 centered 鈥 quarter, obtaining a value of 53,01 for the carob tree, besides knowing the floristic composition of the forest, taking as main reference the ACR Huacrupe. In second place and finally, surveys and interviews with key players, park rangers and local people applied who confirmed the importance of the species to the maintenance of dry forests and as a contribution and economic support to various communities as well as the worrying situation of deforestation, illegal primarily for commercial purposes, habitat fragmentation, degradation and continuous reduction of carob trees.Tesi

    Desarrollo de papeles biocativos por injerto de mol茅culas espec铆ficas en celulosa

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    Tesis (DCI)--FCEFN-UNC, 2019En la presente tesis se presenta el desarrollo de papeles bioactivos con potencial aplicaci贸n en el envasado activo de alimentos. Para tal fin, se propuso el injerto de eugenol, un compuesto de origen natural con propiedades antimicrobiana, antioxidante y repelente de insectos, en celulosa, utilizando 谩cido policarbox铆lico como agente ligante. Con el objetivo de evaluar la escalabilidad del proceso propuesto, se estudiaron distintas tecnolog铆as de curado, tales como calentamiento por convecci贸n, infrarrojo, microondas y conducci贸n. En todos los casos, se analizaron la influencia de las variables operativas sobre el avance de la reacci贸n y propiedades finales del papel preparado, utilizando un dise帽o de experimentos Doehlert para elegir las experiencias a realizar, y analizando los resultados mediante metodolog铆a de superficie de respuesta y an谩lisis estad铆stico ANOVA. Se pudo comprobar que la reacci贸n de injerto de eugenol en papel comercial se produjo con 茅xito en todas las tecnolog铆as estudiadas. Asimismo, se encontraron las condiciones 贸ptimas de reacci贸n para cada una de las tecnolog铆as, para lo cual se busc贸 un compromiso entre el avance de la reacci贸n y las propiedades finales del material (mec谩nicas y color). A partir de estas condiciones, se prepararon papeles y se realiz贸 una caracterizaci贸n m谩s espec铆fica para su aplicaci贸n como envase de alimentos comparando los papeles modificados con el papel virgen. Se analizaron las propiedades mec谩nicas por ensayo de tracci贸n, rasgado y punzonado y se midi贸 la absorci贸n de agua y la capacidad de degradaci贸n. Por otro lado, las propiedades bioactivas analizadas fueron la actividad antioxidante, antimicrobiana, repelente e insecticida de gorgojos (T. castaneum y R. dominica). Una vez probado que el papel modificado presenta buenas caracter铆sticas f铆sicas y bioactivas para su posible aplicaci贸n en el envasado de alimentos, se realizaron prototipos de envasado para harina, como alimento representativo de alimentos derivados de cereales, susceptibles al ataque de plagas. En este estudio se analiz贸 la migraci贸n de reactivos, propiedades organol茅pticas y conservaci贸n del alimento, arrojando resultados promisorios para la industria de envases de alimentos. Finalmente, se realiz贸 una comparaci贸n de las tecnolog铆as de curado ensayadas, analizando diferentes aspectos como avance de reacci贸n, propiedades finales, apariencia, tiempo de reacci贸n, consumo de energ铆a, entre otros, como as铆 tambi茅n disponibilidad y uso de estas tecnolog铆as a escala industrial, seleccionando la tecnolog铆a de conducci贸n como la m谩s adecuada para una propuesta de escalado industrial.Fil: Muratore, Florencia. Universidad Nacional de C贸rdoba. Facultad de Ciencias Exactas, F铆sicas y Naturales; Argentina.Fil: Muratore, Florencia. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Instituto de Investigaci贸n y Desarrollo en Ingenier铆a de Procesos y Qu铆mica Aplicada; Argentina

    El estatus jur铆dico del lobo ib茅rico en el punto de mira : un cambio de paradigma en Castilla y Le贸n = The legal status of the iberian wolf in point of view : a change of paradigm in Castilla y Le贸n

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    40 p谩ginasLa situaci贸n jur铆dica del lobo ib茅rico en Castilla y Le贸n en los 煤ltimos tiempos ha sufrido una profunda modificaci贸n normativa. Son muchos los cambios producidos en su r茅gimen jur铆dico, principalmente en su catalogaci贸n al norte del r铆o Duero en Castilla y Le贸n, donde es especie cineg茅tica. Si bien la suspensi贸n cautelar de su caza al norte del precitado r铆o, decretada por Auto de 26 de abril de 2018, a lo que sigui贸 la urgente aprobaci贸n de la Ley 9/2019, de 28 de marzo, de modificaci贸n de la Ley 4/1996, de 12 de julio, de Caza de la comunidad aut贸noma de Castilla y Le贸n, as铆 como los 煤ltimos pronunciamientos jurisprudenciales en la materia, han motivado un cambio de paradigma en la consideraci贸n jur铆dica del lobo ib茅rico en Castilla y Le贸n. El estudio aborda la actual situaci贸n jur铆dica de la especie en nuestra Comunidad Aut贸noma a partir del an谩lisis jurisprudencial de la responsabilidad patrimonial por los da帽os que causa, rese帽ando las diferencias m谩s notables al respecto en funci贸n de la ubicaci贸n de sus ejemplares al norte o sur del r铆o Duero en Castilla y Le贸nS

    Application of lactic acid bacteria for the biopreservation of meat products: A systematic review

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    .The increasing concern of consumers about food quality and safety and their rejection of chemical additives has promoted the breakthrough of the biopreservation field and the development of studies on the use of beneficial bacteria and their metabolites as potential natural antimicrobials for shelf life extension and enhanced food safety. Control of foodborne pathogens in meat and meat products represents a serious challenge for the food industry which can be addressed through the intelligent use of bio-compounds or biopreservatives. This article aims to systematically review the available knowledge about biological strategies based on the use of lactic acid bacteria to control the proliferation of undesirable microorganisms in different meat products. The outcome of the literature search evidenced the potential of several strains of lactic acid bacteria and their purified or semi-purified antimicrobial metabolites as biopreservatives in meat products for achieving longer shelf life or inhibiting spoilage and pathogenic bacteria, especially when combined with other technologies to achieve a synergistic effect.S

    Study on the concordance between different SNP鈥恎enotyping platforms in sheep

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    .Different SNP genotyping technologies are commonly used in multiple studies to perform QTL detection, genotype imputation, and genomic predictions. Therefore, genotyping errors cannot be ignored, as they can reduce the accuracy of different procedures applied in genomic selection, such as genomic imputation, genomic predictions, and false-positive results in genome-wide association studies. Currently, whole-genome resequencing (WGR) also offers the potential for variant calling analysis and high-throughput genotyping. WGR might overshadow array-based genotyping technologies due to the larger amount and precision of the genomic information provided; however, its comparatively higher price per individual still limits its use in larger populations. Thus, the objective of this work was to evaluate the accuracy of the two most popular SNP-chip technologies, namely, Affymetrix and Illumina, for high-throughput genotyping in sheep considering high-coverage WGR datasets as references. Analyses were performed using two reference sheep genome assemblies, the popular Oar_v3.1 reference genome and the latest available version Oar_rambouillet_v1.0. Our results demonstrate that the genotypes from both platforms are suggested to have high concordance rates with the genotypes determined from reference WGR datasets (96.59% and 99.51% for Affymetrix and Illumina technologies, respectively). The concordance results provided in the current study can pinpoint low reproducible markers across multiple platforms used for sheep genotyping data. Comparing results using two reference genome assemblies also informs how genome assembly quality can influence genotype concordance rates among different genotyping platforms. Moreover, we describe an efficient pipeline to test the reliability of markers included in sheep SNP-chip panels against WGR datasets available on public databases. This pipeline may be helpful for discarding low-reliability markers before exploiting genomic information for gene mapping analyses or genomic predictionS

    What is the importance of sperm subpopulations?

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    .The study of sperm subpopulations spans three decades. The origin, meaning, and practical significance, however, are less clear. Current technology for assessing sperm morphology (CASA-Morph) and motility (CASA-Mot) has enabled the accurate evaluation of these features, and there are many options for data classification. Subpopulations could occur as a result of the stage of development of each spermatozoon in the subpopulation. Spermatogenesis might contribute to the production of these subpopulations. Insights from evolutionary biology and recent molecular research are indicative of the diversity among male gametes that could occur from unequal sharing of transcripts and other elements through cytoplasmic bridges between spermatids. Sperm cohorts exiting the gonads would contain different RNA and protein contents, affecting the spermatozoon physiology and associations with the surrounding environmental milieu. Subsequently, these differences could affect how spermatozoa interact with the environmental milieu (maturation, mixing with seminal plasma, and interacting with the environmental milieu, or female genital tract and female gamete). The emergence of sperm subpopulations as an outcome of evolution, related to the reproductive strategies of the species, genital tract structures, and copulatory and fertilization processes. This kind of approach in determining the importance of sperm subpopulations in fertilization capacity should have a practical impact for conducting reproductive technologies, inspiring and enabling new ways for the more efficient use of spermatozoa in the medical, animal breeding, and conservation fields. This manuscript is a contribution to the Special Issue in memory of Dr. Duane GarnerS

    A new index of resilience applicable to external pulse-disturbances that considers the recovery of communities in the short term

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    .Resilience is a key concept in the study of the recovery of ecosystems affected by disturbances. Currently, there are numerous indices to measure resilience, but many of them do not show the accuracy of the resilience value or the behaviour of ecological parameters in the face of disturbances. New approaches and technologies enable large amounts of information to be obtained, facilitating the proposal of new resilience indices that work consistently and intuitively for a wide variety of ecological response variables under different scenarios after pulse-disturbances. In this study, we propose and verify a new resilience index, comparing its performance with others previously published. We validated the performance of the new index using real data based on field measurements of changes in soil bacterial OTUs diversity and abundance after a wildfire. The new resilience index provided an automatic and robust functional classification of the behaviour of ecosystems after disturbances, supported by a bootstrap analysis. We identified 5 scenarios of ecosystem resilience performance according to their behaviour after a pulse-disturbance: resilient, non-resilient, recovering, rebound, and continuing.S

    Identification of Hindbrain Neural Substrates for Motor Initiation in the hatchling Xenopus laevis Tadpole

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    Animal survival profoundly depends on the ability to detect stimuli in the environment, process them and respond accordingly. In this respect, motor responses to a sensory stimulation evolved into a variety of coordinated movements, which involve the control of brain centres over spinal locomotor circuits. The hatchling Xenopus tadpole, even in its embryonic stage, is able to detect external sensory information and to swim away if the stimulus is considered noxious. To do so, the tadpole relies on well-known ascending sensory pathway, which carries the sensory information to the brain. When the stimulus is strong enough, descending interneurons are activated, leading to the excitation of spinal CPG neurons, which causes the undulatory movement of swimming. However, the activation of descending interneurons that marks the initiation of motor response appears after a long delay from the sensory stimulation. Furthermore, the long-latency response is variable in time, as observed in the slow-summating excitation measured in descending interneurons. These two features, i.e. long-latency and variability, cannot be explained by the firing time and pattern of the ascending sensory pathway of the Xenopus tadpole. Therefore, a novel neuronal population has been proposed to lie in the hindbrain of the tadpole, and being able to 'hold' the sensory information, thus accounting for the long and variable delay of swim initiation. In this work, the role of the hindbrain in the maintenance of the long and variable response to trunk skin stimulation is investigated in the Xenopustadpole at developmental stage 37/38. A multifaceted approach has been used to unravel the neuronal mechanisms underlying the delayed motor response, including behavioural experiments, electrophysiology analysis of fictive swimming, hindbrain extracellular recordings and imaging experiments. Two novel neuronal populations have been identified in the tadpole's hindbrain, which exhibit activation patterns compatible with the role of delaying the excitation of the spinal locomotor circuit. Future work on cellular properties and synaptic connections of these newly discovered populations might shed light on the mechanism of descending control active at embryonic stage. Identifying supraspinal neuronal populations in an embryonic organism could aid in understanding mechanisms of descending motor control in more complex vertebrates

    Bioinformatic characterization of a triacylglycerol lipase produced by Aspergillus flavus isolated from the decaying seed of Cucumeropsis mannii

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    Lipases are enzymes of industrial importance responsible for the hydrolysis of ester bonds of triglycerides. A lipolytic fungus was isolated and subsequently identified based on the ITS sequence analysis as putative Aspergillus flavus with accession number LC424503. The gene coding for extracellular triacylglycerol lipase was isolated from Aspergillus flavus species, sequenced, and characterised using bioinformatics tools. An open reading frame of 420 amino acid sequence was obtained and designated as Aspergillus flavus lipase (AFL) sequence. Alignment of the amino acid sequence with other lipases revealed the presence GHSLG sequence which is the lipase consensus sequence Gly-X1-Ser-X2-Gly indicating that it a classical lipase. A catalytic active site lid domain composed of TYITDTIIDLS amino acids sequence was also revealed. This lid protects the active site, control the catalytic activity and substrate selectivity in lipases. The 3-Dimensional structural model shared 34.08% sequence identity with a lipase from Yarrowia lipolytica covering 272 amino acid residues of the template model. A search of the lipase engineering database using AFL sequence revealed that it belongs to the class GX-lipase, superfamily abH23 and homologous family abH23.02, molecular weight and isoelectric point values of 46.95鈥塊Da and 5.7, respectively. N-glycosylation sites were predicted at residues 164, 236 and 333, with potentials of 0.7250, 0.7037 and 0.7048, respectively. O-glycosylation sites were predicted at residues 355, 358, 360 and 366. A signal sequence of 37 amino acids was revealed at the N-terminal of the polypeptide. This is a short peptide sequence that marks a protein for transport across the cell membrane and indicates that AFL is an extracellular lipase. The findings on the structural and molecular properties of Aspergillus flavus lipase in this work will be crucial in future studies aiming at engineering the enzyme for biotechnology applications

    Uso de las histonas circulantes y sus modificaciones post-traduccionales como biomarcadores en sepsis y shock s茅ptico

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    La sepsis es una afecci贸n potencialmente mortal causada por una respuesta anormal del hu茅sped a una infecci贸n, produciendo respuestas fisiol贸gicas alteradas que da帽an los propios tejidos del paciente y pueden provocar disfunci贸n org谩nica e incluso la muerte. Asimismo, algunos pacientes s茅pticos progresan a shock s茅ptico, caracterizado por alteraciones circulatorias, celulares y metab贸licas sustanciales que aumentan el riesgo de mortalidad. A pesar de que la sepsis se caracteriza por un mal funcionamiento del sistema inmunol贸gico, lo que a su vez conduce a una respuesta inmune alterada e inmunosupresi贸n, la alta complejidad de la fisiopatolog铆a de la sepsis requiere una mayor investigaci贸n para comprender las respuestas inmunes que ocurren durante la sepsis. Asimismo, las histonas extracelulares circulantes han ganado relevancia como mediadores citot贸xicos en la sepsis, ya que act煤an como patrones moleculares asociados a da帽o, que inducen estr茅s oxidativo y activan el inflamasoma NLRP3. Estos mecanismos median la activaci贸n de la piroptosis, un mecanismo de muerte celular programada que produce inflamaci贸n mediante la expresi贸n de IL-18, IL-1尾 and IL-1伪. Sin embargo, a pesar de la evidencia de activaci贸n del inflamasoma en las c茅lulas inmunes durante la sepsis, se desconoce si las histonas extracelulares son capaces de activar los inflamasomas endoteliales y sus consecuencias. En este trabajo destacamos el papel previamente desconocido de las histonas extracelulares, mediando la activaci贸n del inflamasoma NLRP3 y la piroptosis en las c茅lulas endoteliales, contribuyendo a la disfunci贸n endotelial y la desregulaci贸n de la respuesta inmune mediada por el endotelio. Asimismo, tambi茅n demostramos c贸mo la acetilaci贸n de histonas disminuye la activaci贸n de la piroptosis. Adem谩s, demostramos que la piroptosis se produce en pacientes con shock s茅ptico y los niveles de histonas circulantes se correlacionan con la expresi贸n de citoquinas proinflamatorias y citoquinas piropt贸ticas, la liberaci贸n de factores de adhesi贸n endotelial y la gravedad de la enfermedad. Proponemos la piroptosis mediada por histonas como un nuevo objetivo para desarrollar intervenciones cl铆nicas. De manera similar, hemos analizado las respuestas inmunorelacionadas que ocurren durante las primeras etapas de la sepsis con el objetivo de proporcionar nuevos datos comparando las cantidades de citoquinas, inmunomoduladores y otros mediadores endoteliales en pacientes cr铆ticamente enfermos no s茅pticos, s茅pticos y de shock s茅ptico. Nuestro enfoque ayudar谩 a caracterizar r谩pidamente las respuestas inmunes alteradas en pacientes s茅pticos y de shock s茅ptico ingresados en la Unidad de Cuidados Intensivos. Finalmente analizamos el papel de la metilaci贸n del ADN en el control del sistema inmune s茅ptico. Nuestros resultados demostraron el papel central de la metilaci贸n del ADN modulando la respuesta molecular en los pacientes de shock s茅ptico y contribuyendo a la inmunosupresi贸n, a trav茅s de la alteraci贸n de los patrones de metilaci贸n de los promotores de IL-10 y TREM-2.Sepsis is a life-threatening condition caused by an abnormal host response to an infection that produce altered physiological responses which damages own tissues of the patient and can result in organ dysfunction and in some cases death. Likewise, a subset of septic patients progresses to septic shock, characterized by substantial circulatory, cellular and metabolic abnormalities, which substantially increase the risk of mortality. Sepsis is characterized by a malfunction of the immune system and it can lead to an altered immune response and immunosuppression. Moreover, the high complexity of the pathophysiology of sepsis requires of further investigation to characterize the immune responses in sepsis and septic shock. Likewise, circulating extracellular histones have gained relevance as cytotoxic mediators in sepsis pathophysiology, since they act as damage-associated molecular patterns, which induce oxidative stress and activate NLRP3 inflammasome. Subsequently, inflammasome mediates pyroptosis activation, a programmed cell death mechanism that produces inflammation through the release of IL-18, IL-1尾 and IL-1伪. However, despite inflammasome activation may occur in immune cells during sepsis, it is unknown if this process also takes place in endothelial cells and particularly whether extracellular histones are capable of activating endothelial inflammasomes and their consequences. In this work we highlight a previously unknown role for extracellular histones, that mediates the activation of NLRP3 inflammasome and pyroptosis in endothelial cells by contributing to endothelial dysfunction and the dysregulation of the immune response mediated by endothelium. Likewise, we demonstrated how histone acetylation decreases pyroptosis activation. Furthermore, we show how pyroptosis occurs in septic shock patients and how circulating histone levels correlate with the expression of pro-inflammatory and pyroptotic cytokines, the release of endothelial adhesion factors and septic shock severity. We propose histone-mediated pyroptosis as a new target to develop clinical interventions. Similarly, we have analyzed the immune-related responses occurring during the early stages of sepsis with the aim of providing new data by comparing the amounts of cytokines, immune modulators and other endothelial mediators in critically-ill non-septic patients, septic and septic shock patients. Our approach will help to rapidly characterize the altered immune responses in septic and septic shock patients admitted in the Intensive Care Unit. Finally, we also analyzed the role of DNA methylation in the control of septic immune system. Our results demonstrated the central role of DNA methylation modulating the molecular response in septic shock patients and contributing to immunosuppression, through the alteration of DNA methylation patterns of IL-10 and TREM2 promoters
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