2 research outputs found
High occurrence of Pacearchaeota and Woesearchaeota (Archaea superphylum DPANN) in the surface waters of oligotrophic high-altitude lakes
8 páginas, 4 figuras, 1 tabla.We carried out a regional survey on the archaea composition
from surface waters of > 300 high-altitude
Pyrenean lakes (average altitude 2300 m, pH range
4.4–10.1) by 16S rRNA gene tag sequencing. Relative
Archaea abundances ranged between 0% and 6.3% of
total prokaryotes amplicons in the polymerase chain
reaction (PCR) mixture, and we detected 769 operational
taxonomic units (OTUs; grouped at 97% identity)
that split into 13 different lineages, with altitude
and pH having a significant effect on the community
composition. Woesearchaeota and Pacearchaeota
(formerly Euryarchaeota DHVEG-6 cluster) dominated
the data set (83% of total OTUS), showed a high
occurrence (presence in c. 75% of the lakes) and
had relative abundances significantly and positively
correlated with the phylogenetic diversity of
bacterial communities. Micrarchaeota–Diapherotrites
(formerly Euryarchaeota MEG cluster), Methanomicrobia,
Thermoplasmata and ammonia-oxidizing
thaumarchaeota (AOA) showed relative abundances
between 1% and 3% and occurrences between
14% and 26%. Minor lineages were SM1K20,
Aenigmarchaeota (formerly Euryarchaeota DSEG
cluster), Methanobacteria, Bathyarchaeota and
SCG. Environmental preferences substantially differed
among lineages, with Aenigmarchaeota and
Methanomicrobia having the largest habitat breadth,
and Thermoplasmata, AOA and Micrarchaeota having
the smallest. Pacearchaeota and Woesearchaeota
had been mostly reported from saline habitats and
sediments, but surface waters of oligotrophic alpine
lakes are suitable environments for such ecologically
spread and genetically diverse archaeal lineages.Funding was
provided by grants DARKNESS CGL2012-32747 from the
Spanish Office of Science (MINECO) and European funding
(ERDF), and DISPERSAL 829/2013 from the OAPN-Spanish
National Parks Network (MAGRAMA) to EOC. ROA was
funded by the Spanish FPI PhD scholarships program
(MINECO).Peer reviewe