Two Dimensional Electrophoresis of Galactosidase Relating to the Disappearance of Bombyx Lectin Activity

"Two dimensional polyacrylamide gel electroporesis (2 D-PAGE) analysis on the haemolymph of Bombyx mori was performed using the Mini-PROTEAN mini tube gel two dimensional polyacrylamide gel electrophoresis system (Bio-Rad Laboratories, Inc.). The result on various electrophoretical conditions using the haemolymph-protein showed the possibility that the haemolymph-protein was separated actually by means of this method. Moreover, the result of 2 D-PAGE analysis on Fraction II obtained by gel filtration from the haemolymph of the domesticated silkworm of Bombyx mori showed that the fraction seemed to be separated by two dimensional polyacrylamide gel electrophoresis. This research was partly supported by a grant from Tezukayama Gakuen.

An Approach for Proteomic Analysis of Bombyx Humoral Lectin Related Proteins using Two-Dimensional Gel Electrophoresis

"Comparative study of two-dimensional polyacrylamide gel electrophoresis (2 D-PAGE) was performed on the hemolymph and the fat body of the domesticated silkworm, Bombyx mori. The result showed that both the larval hemolymph on day 10 in the fifth instar and the pupal hemolymph on day 3 after pupation were obtained distinct 2 D-PAGE profiles. However, faint 2 D-PAGE profiles were obtained for both the larval fat body and the pupal fat body. Similar results were obtained on the Fraction II from the larval hemolymph and from the larval fat body, i.e., clearer 2-D PAGE profile of Fraction II was obtained from the larval hemolymph than the larval fat body. This research was partly supported by a grant from Tezukayama Gakuen.

Mass Spectrometric Analysis of Bombyx mori Humoral Lectin Related Proteins

"Two spots of 2-D PAGE on Fraction II obtained by gel filtration from the larval hemolymph of the Bombyx mori were excised from the gels and subjected to in-gel tryptic digestion, MALDITOF MS analysis, and a SWISS-PROT protein database search. The result suggested that spot 1 obtained by 2-D PAGE was identified as probably GDP-mannose 4, 6-dehydratase (EC4.2.1.47), which is described as guanoshin 5\u27-diphosphoric acid-D-mannose dehydration enzyme. On the other hand, spot 2 obtained by 2-D PAGE was identified as β-lactamase (EC3.5.2.6) which is known as a hydrolase to lose the anti-bacillus by rending the β-lactam ring. However, the reason why these enzymes exist in the silkworm\u27s hemolymph is still unknown, and to elucidate it will be a problem for future study.

On Flip-Flop Selection for Multi-cycle Scan Test with Partial Observation in Logic BIST

Multi-cycle test with partial observation for scan-based logic BIST is known as one of effective methods to improve fault coverage without increase of test time. In the method, the selection of flip-flops for partial observation is critical to achieve high fault coverage with small area overhead. This paper proposes a selection method under the limitation to a number of flip-flops. The method consists of structural analysis of CUT and logic simulation of test vectors, therefore, it provides an easy implementation and a good scalability. Experimental results on benchmark circuits show that the method obtains higher fault coverage with less area overhead than the original method. Also the relation between the number of selected flip-flops and fault coverage is investigated.27th IEEE ASIAN TEST SYMPOSIUM (ATS\u2718), 15-18 October 2018, Hefei, Chin

A Flexible Power Control Method for Right Power Testing of Scan-Based Logic BIST

High power dissipation during scan-based logic BIST is a crucial problem that leads to over-testing. Although controlling test power of a circuit under test (CUT) to an appropriate level is strongly required, it is not easy to control test power in BIST. This paper proposes a novel power controlling method to control the toggle rate of the patterns to an arbitrary level by modifying pseudo random patterns generated by a TPG (Test Pattern Generator) of logic BIST. While many approaches have been proposed to control the toggle rate of the patterns, the proposed approach can provide higher fault coverage. Experimental results show that the proposed approach can control toggle rates to a predetermined target level and modified patterns can achieve high fault coverage without increasing test time.2016 IEEE 25th Asian Test Symposium (ATS), 21-24 Nov. 2016, Hiroshima, Japa

Massive trapezial metastasis from gastric adenocarcinoma resected and reconstructed with a vascularized scapular bone graft: A case report

Rationale: Isolated metastasis to the hand bones is very rare. Only seven cases of metastasis to the trapezium have been reported. To the best of our knowledge, this is the first report of a single metastasis to the trapezium from a gastric adenocarcinoma. Patient concerns: A 62-year-old man presented with pain and massive swelling in the right carpometacarpal joint of the thumb. Diagnoses: The patient was diagnosed with trapezial metastasis of advanced gastric adenocarcinoma. Interventions: The patient underwent systemic chemotherapy with cisplatin and S-1, radiotherapy to the metastatic bone, and treatment with denosumab. One year later, the huge metastatic tumor was resected, and the hand was reconstructed using vascularized scapular bone. Outcomes: Eighteen months postoperatively, the patient was satisfied with the appearance of the reconstructed hand and was able to use his right thumb in activities of daily living. Lessons: Although rare, metastasis to the trapezium should be considered in patients with persistent and progressive thumb CMC joint pain.ArticleMedicine 96(50) : e9294(2017)journal articl