FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

YÜZEYDE GÜŞLENDİRİLMİŞ RAMAN SPEKTROSKOPİSİ TEMELİNE DAYANAN NANOTEKNOLOJİ DESTEKLİ TBBI TEŞHİS TESTLERİNİN GELİŞTİRİLMES

Türkiye’de kronik B, C ve D hepatit infeksiyonlarında SEN-H virüsünün klinik önemi ve sıklığı

Amaç: SEN virus paranteral yolla bulaşır ve transfüzyon sonrası hepatite neden olabilir. Klinik önemi ve sıklığı kronik C ve B hepatitli hastalarda araştırılmıştır, ancak kronik D hepatitinde önemi bilinmemektedir. Bu çalışmada amaç Türkiye’de kronik C, B ve delta hepatitinde SEN virus-H sıklığı ve klinik önemini belirlemektir. Yöntem: Seksen beş kronik viral hepatitli hasta (30 HCV, 30 HBV ve 25 HDV) ve 43 sağlıklı kan vericisinde SEN virus-H araştırılmıştır. Hepatit B, C ve D hastaları nda sırasıyla HBV DNA, HCV RNA ve HDV RNA pozitif idi. SEN virus-H DNA’sı serumdan DNA ekstraksiyonu yapıldı ktan sonra (NucleoSpin blood; Macherey-Nagel GmbH & Co KG, Germany) seminested polimeraz zincir reaksiyonu ile (L2AS, C5S primer in first step, L2AS, D11 in second step) araştırılmıştır. Bulgular: SEN virus-H DNA pozitifliği, kronik C, B, D hepatitinde ve sağlıklı kan vericilerinde sırasıyla 7/30(23.3%), 10/30 (33.3%), 6/25(24%), 7/43(16.2%) olarak saptandı. Kronik viral hepatitli hastalarda, SEN virus-H pozitif ve negatif hastalar arasında klinik özellikler ve tedaviye cevap açısından farklılık saptanmadı. Sonuç: SEN virus kronik viral hepatitli hastalarda sağlıklı kan vericilerinden daha fazladır. Bu sonuçlar, SEN virusun kronik viral hepatitde tedavi cevabı ve klinik seyirde etkili olmadığı nı göstermektedir.Background/aims: SEN viruses are transmitted parenterally and can cause post-transfusion hepatitis. The prevalence and clinical significance of SEN viruses have been investigated in patients with chronic hepatitis C and B but not in D. We aimed to determine the prevalence and clinical significance of SEN viruses- H in patients with chronic hepatitis C, B and delta in Turkey. Methods: SEN viruses-H was analyzed in 85 patients with chronic viral hepatitis (30 HCV, 30 HBV and 25 HDV) and 43 non-professional blood donors. HBV DNA, HCV RNA and HDV RNA were positive in patients with hepatitis B, C and D, respectively. SEN viruses-H DNA was detected by semi-nested polymerase chain reaction method (L2AS, C5S primer in first step, L2AS, D11 in second step) after extraction of DNA from sera (NucleoSpin blood; Macherey-Nagel GmbH & Co KG, Germany). Results: SEN viruses-H DNA was found to be positive in 7/30 (23.3%), 10/30 (33.3%), 6/25 (24%), and 7/43 (16.2%) of patients with chronic C, B, and D hepatitis and healthy blood donors, respectively. There was no significant difference in clinical features and treatment response between SEN viruses- H-positive and -negative patients with chronic viral hepatitis. Conclusions: SEN viruses is more frequent in chronic hepatitis patients than in healthy blood donors. These results indicate that SEN viruses has no effect on the clinical course and treatment response of chronic viral hepatitis

Decreased Serum 25-hydroxyvitamin D Level Causes Interventricular Septal Hypertrophy in Patients on Peritoneal Dialysis: Cardiovascular Aspects of Endogenous Vitamin D Deficiency

Introduction. In the present study, we aimed to analyze the relation of vitamin D with echocardiographic indexes in patients with end stage renal disease (ESRD) receiving renal replacement therapy (RRT). Methods. A total of 98 patients, 64 patients on hemodialysis (HD) (29F/35M, mean age 56.75 ± 18.63 years) and 34 age matched patients on peritoneal dialysis (PD) (21F/13M, mean age 58.11 ± 10.63 years), with similar duration of ESRD and RRT were enrolled into this cross-sectional study. Echocardiographic examination was performed after dialysis session at normovolemic status. Fasting blood samples were obtained before dialysis session. Results. Patients on PD and female patients in both groups had significantly lower level of 25-OH-D3 level when compared to patients on HD or male patients (p: 0.0001 and p: 0.0001). When all participants were considered, there was no significant association between 25-OH-D3 and echocardiographic parameters; however, in patients on PD, a significant negative correlation was determined between 25-OH-D3 and diastolic blood pressure, interventricular septal hypertrophy (ISH), and left ventricular mass index (LVMI) (r: −0.424, p: 0.012; r: −0.508, p: 0.004; r: 0.489, p: 0.04, resp.). Conclusion. Low serum 25-hydroxyvitamin D levels is associated with ISH and LVMI in PD patients

Demographic, clinical and laboratory characteristics of adult-onset minimal change disease in Turkey: Turkish Society of Nephrology-Glomerular Diseases (TSN-GOLD) Working Group

Purpose In our study, diagnostic and demographic characteristics of patients diagnosed with minimal change disease (MCD) by biopsy, clinical and laboratory findings in our country were investigated. Methods Data were obtained from the Turkish Society of Nephrology Glomerular Diseases (TSN-GOLD) Working Group database. Demographic characteristics, indications for biopsy, diagnosis of the glomerular diseases, comorbidities, laboratory and biopsy findings of all patients were recorded. The data presented are cross-sectional and includes application data for the biopsy period. Results Of 3875 patients, 233 patients with MCD (median age 35.0 years) were included in the study, which constitutes 6.0% of the total glomerulonephritis database. Renal biopsy was performed in 196 (84.1%) patients due to nephrotic syndrome. Median serum creatinine was 0.7 (0.6-1.0) mg/dl, mean eGFR was 104 +/- 33 ml/min/1.73 m(2) and median proteinuria 6000 mg/day. The number of patients under the age of 40 years was 139 (59.7%) (Group A), and the number of patients aged 40 years and over was 94 (40.3%) (Group B). Compared to Group A, global sclerotic glomeruli (24 vs. 43, p < 0.001) interstitial inflammation (15 vs. 34, p < 0.001), interstitial fibrosis (20 vs. 31, p = 0.001, vascular changes (10 vs. 25, p < 0.001) and tubular atrophy (18 vs. 30, p < 0.001) were found to be significantly higher in Group B. There was no difference in immunofluorescent staining properties between the two groups. Conclusion Our data are generally compatible with the literature. Chronic histopathological changes were more common in patients aged 40 years and older than younger patients. Studies investigating the effects of these different features on renal survival are needed

The prevalence, clinical and pathological characteristics of primary focal segmental glomerulosclerosis in Turkish adults: The data from TSN-Gold (Turkish society of nephrology glomerular diseases) working group

...ERA; EDT

Characteristics of primary glomerular diseases patients with hematuria in Turkey: The data from TSN-GOLD Working Group

Purpose Hematuria is one of the most common laboratory findings in nephrology practice. To date, there is no enough data regarding the clinical and histopathologic characteristics of primary glomerular disease (PGD) patients with hematuria in our country. Methods Data were obtained from national multicenter (47 centers) data entered into the Turkish Society of Nephrology Glomerular Diseases (TSN-GOLD) database between May 2009 and June 2019. The data of all PGD patients over the age of 16 years who were diagnosed with renal biopsy and had hematuria data were included in the study. Demographic characteristics, laboratory and biopsy findings were also recorded. Results Data of 3394 PGD patients were included in the study. While 1699 (50.1%) patients had hematuria, 1695 (49.9%) patients did not have hematuria. Patients with hematuria had statistically higher systolic blood pressure, serum blood urea nitrogen, creatinine, albumin, levels and urine pyuria. However, these patients had statistically lower age, body mass index, presence of hypertension and diabetes, eGFR, 24-h proteinuria, serum total, HDL and LDL cholesterol, and C3 levels when compared with patients without hematuria. Hematuria was present 609 of 1733 patients (35.8%) among the patients presenting with nephrotic syndrome, while it was presented in 1090 of 1661 (64.2%) patients in non-nephrotics (p < 0.001). Conclusion This is the first multicenter national report regarding the demographic and histopathologic data of PGD patients with or without hematuria. Hematuria, a feature of nephritic syndrome, was found at a higher than expected in the PGDs presenting with nephrotic syndrome in our national database

Adult minimal change disease in Turkey: The results of Turkish society of nephrology glomerular diseases working group

...ERA EDT

Characteristics of primary glomerular disease patients with hematuria in Turkey: The data from TSN-Gold working group

...ERA EDT