Creating “S”itizenship, Denying Citizenship: A Hmong Man’s Journey through High School

This article explores the perspective of a Hmong student’s experience in high school in the context of patriotism and citizenship. Growing up and receiving Citizenship Awards, he noticed the contrast with the Citizenship Room at his high school. Citizenship was given through these awards and is taken away in the Citizenship Room. This article critiques the author’s nostalgia for high school as he takes on a different perspective and asks himself “How did being Asian affect my experience?” and “Would I still have nostalgia for high school if the Citizenship Room was constantly part of my experience?

Introduction

Introduction to volume 6 of Macalester College\u27s journal Tapestries: Interwoven voices of local and global identities

Laser-assisted photoporation : fundamentals, technological advances and applications

Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-of-the-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticle-mediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts

Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target site. However, there are only limited methods available that allow determining the stability of genetic materials in biological environments. In this context, the decay kinetics of fluorescently labeled plasmid DNA (pDNA) and messenger RNA (mRNA) in undiluted biological samples (i.e., human serum, human ascites, bovine vitreous) and cell extracts is studied using fluorescence correlation spectroscopy (FCS) and single particle tracking (SPT). It is demonstrated that FCS is suitable to follow mRNA degradation, while SPT is better suited to investigate pDNA integrity. The half-life of mRNA and pDNA is approximate to 1-2 min and 1-4 h in biological samples, respectively. The resistance against biodegradation drastically improves by complexation with lipid-based carriers. Taken together, FCS and SPT are able to quantify the integrity of mRNA and pDNA, respectively, as a function of time, both in the extracellular biological fluids and cell extracts. This in turn allows to focus on the important but less understood issue of nucleic acids degradation in more detail and to rationally optimize gene delivery system as therapeutics

MEMS 411: Heavy Cart Ramp Assistant

Dr. Katharine Flores has a heavy Argon tank that needs to be put on a scale and wants a device that a single person can operate to assist them in that task

Effects of Time-Varying Relative Spectral Response on the Calibration of MODIS Reflective Solar Bands

Calibration of the on-orbit gain changes of the narrow bandwidth reflective solar bands (RSB) of Terra and Aqua MODIS is usually based on the band center wavelength. The relative spectral response (RSR) of each band is assumed to be constant on orbit and the time dependence of an overall gain factor is calculated. Any on-orbit changes to the RSR of the MODIS bands will introduce some error into the calibration and may also have an impact on the Earth scene radiance retrieval. We consider two different ways to track how the RSR of the MODIS RSB may be changing on orbit, and the effect that these changes will have on the calibration. First, we examine in-band RSR measurements from the spectro-radiometric calibration assembly (SRCA) carried on-board both MODIS instruments. Second, we study the broadband degradation of the MODIS scan mirror and how it may be changing the effective out-of-band response of the RSB. We find that RSR changes have a small effect on the radiance calibrated using the on-board solar diffuser, generally less than 0.5% in all cases at any time in the missions, with bands 1, 8, and 9 impacted the most

Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Sizing nanomaterials in complex biological fluids, such as blood, remains a great challenge in spite of its importance for a wide range of biomedical applications. In drug delivery, for instance, it is essential that aggregation of protein-based drugs is avoided as it may alter their efficacy or elicit immune responses. Similarly it is of interest to determine which size of molecules can pass through biological barriers in vivo to diagnose pathologies, such as sepsis. Here, we report on continuous fluorescence recovery after photobleaching (cFRAP) as a analytical method enabling size distribution measurements of nanomaterials (1-100 nm) in undiluted biological fluids. We demonstrate that cFRAP allows to measure protein aggregation in human serum and to determine the permeability of intestinal and vascular barriers in vivo. cFRAP is a new analytical technique that paves the way towards exciting new applications that benefit from nanomaterial sizing in bio-fluids

SNPP VIIRS RSB Earth View Reflectance Uncertainty

The Visible Infrared Imaging Radiometer Suite (VIIRS) on the Suomi National Polar-orbiting Partnership (SNPP) satellite uses its 14 reflective solar bands to passively collect solar radiant energy reflected off the Earth. The Level 1 product is the geolocated and radiometrically calibrated top-of- the-atmosphere solar reflectance. The absolute radiometric uncertainty associated with this product includes contributions from the noise associated with measured detector digital counts and the radiometric calibration bias. Here, we provide a detailed algorithm for calculating the estimated standard deviation of the retrieved top-of-the-atmosphere spectral solar radiation reflectance

A/B Testing and Best-arm Identification for Linear Bandits with Robustness to Non-stationarity

We investigate the fixed-budget best-arm identification (BAI) problem for linear bandits in a potentially non-stationary environment. Given a finite arm set $\mathcal{X}\subset\mathbb{R}^d$, a fixed budget $T$, and an unpredictable sequence of parameters $\left\lbrace\theta_t\right\rbrace_{t=1}^{T}$, an algorithm will aim to correctly identify the best arm $x^* := \arg\max_{x\in\mathcal{X}}x^\top\sum_{t=1}^{T}\theta_t$ with probability as high as possible. Prior work has addressed the stationary setting where $\theta_t = \theta_1$ for all $t$ and demonstrated that the error probability decreases as $\exp(-T /\rho^*)$ for a problem-dependent constant $\rho^*$. But in many real-world $A/B/n$ multivariate testing scenarios that motivate our work, the environment is non-stationary and an algorithm expecting a stationary setting can easily fail. For robust identification, it is well-known that if arms are chosen randomly and non-adaptively from a G-optimal design over $\mathcal{X}$ at each time then the error probability decreases as $\exp(-T\Delta^2_{(1)}/d)$, where $\Delta_{(1)} = \min_{x \neq x^*} (x^* - x)^\top \frac{1}{T}\sum_{t=1}^T \theta_t$. As there exist environments where $\Delta_{(1)}^2/ d \ll 1/ \rho^*$, we are motivated to propose a novel algorithm $\mathsf{P1}$-$\mathsf{RAGE}$ that aims to obtain the best of both worlds: robustness to non-stationarity and fast rates of identification in benign settings. We characterize the error probability of $\mathsf{P1}$-$\mathsf{RAGE}$ and demonstrate empirically that the algorithm indeed never performs worse than G-optimal design but compares favorably to the best algorithms in the stationary setting.Comment: 25 pages, 6 figure

Comparison of MODIS Solar Diffuser Stability Monitor Calibration Results for Different Operational Configurations

The MODIS instruments on the Terra and Aqua spacecraft use a sunlit solar diffuser (SD), with an optional SD attenuation screen (SDS), to calibrate the reflective solar bands. A solar diffuser stability monitor (SDSM) is used to track the SD reflectance degradation on orbit, by taking a ratio of the detector response when viewing the SD compared to the response when viewing the sun. The MODIS SDSMs have been operated both with and without the SDS in place. The SDSMs have also been operated in both a fixed and an alternating mode. In the alternating mode, the SDSM detectors view the SD, sun, and a dark background in an alternating pattern with the view changing on every MODIS scan within a single orbit. In the fixed mode, the SDSM detectors are fixed on the sun view for one orbit, and then are fixed on the SD view for the following orbit. This paper reviews the history of the SDSM operational configurations used throughout the MODIS missions and discusses the differences in the SD degradation results, which may be due to differences in sun-satellite geometry, SD signal level, and stray light effects. We highlight Aqua SDSM results from two recent dates in October 2017 and July 2019, where both the fixed and alternating mode calibrations were run on the same day, providing clear examples of the calibration differences. Additionally, we show how mixing the results from calibrations done with and without the SDS for Aqua MODIS can provide more stable results