Abrasive Weeding as a Vehicle for Precision Fertilizer Management in Organic Vegetable Production

Abrasive weeding is a nonchemical weed control tactic that uses small, gritty materials propelled with compressed air to destroy weed seedlings. Organic fertilizers have been used successfully as abrasive grits to control weeds, but the goal for this study was to explore the effects of fertilizer grit, application rates, and background soil fertility on weeds, plant available nitrogen (N) uptake, and crop yield. Field trials were conducted in organic ‘Carmen’ sweet red pepper (Capsicum annuum) and organic ‘Gypsy’ broccoli (Brassica oleracea var. italica) and treatments included organic fertilizer grit (8N–0.9P–3.3K vs. 3N–3.1P–3.3K), grit application rates (low vs. high), compost amendments (with and without), and weedy and weed-free controls. Weed biomass was harvested at 84 days and 65 days after transplanting for pepper and broccoli, respectively. Simulated total plant available N (nitrate + ammonium) uptake was measured with ion exchange resin stakes between 7 and 49 days after the first of two grit applications. Produce was harvested at maturity, graded for marketability, and weighed. The higher grit application rate, regardless of fertilizer type, reduced the weed biomass by 75% to 89% for pepper and by 86% to 99% for broccoli. By 5 weeks after the first grit application, simulated plant N uptake was greatest following grit application with the 8% N fertilizer, followed by the 3% N fertilizer, and lowest in the weedy control. The high grit application rate of 8% N fertilizer increased pepper yield by 112% compared with the weedy control, but it was similar to that of the weed-free control. Broccoli was less responsive to abrasive grits, with yield changes ranging from no difference to up to a 36% increase (relative to the weedy control) depending on the application rate and compost amendment. This is the first evidence indicating that the nutrient composition of organic fertilizer abrasive grits can influence in-season soil N dynamics, weed competition, and crop yield. The results suggest that abrasive weeding technology could be leveraged to improve the precision of in-season fertilizer management of organic crops

Biobased Sprayable Mulch Films Suppressed Annual Weeds in Vegetable Crops

Biobased sprayable mulch (BSM) films are a potential alternative to herbicides, polyethylene plastic mulch film, and hand weeding for specialty crops. We developed a series of BSM films using locally available biomaterials [including corn (Zea mays) starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, eggshells, and isolated soy (Glycine max) protein] and tested their effects on weeds and crop yield during a total of seven greenhouse or field trials between 2017 and 2019 in Nebraska, USA. Application rates of BSM films applied in pots (greenhouse), planting holes in plastic film (field), or bed tops (field) ranged from 0.9 to 18.2 L⋅m−2; they were applied before and after the emergence of weeds. Weed control efficacy was variable, and results of greenhouse pots were rarely replicated under field conditions. Increasing the viscosity of the final suspension tested [BSM7; a mix of corn starch (72.8 g⋅L−1), glycerol (184.7 mL⋅L−1), keratin hydrolysate (733.3 mL⋅L−1), corn zein (19.8 g⋅L−1), and isolated soy protein (19.8 g⋅L−1)] reduced weed biomass by more than 96% in field-grown kale (Brassica oleracea var. sabellica) when applied to bare soil bed tops before or after weed emergence, but kale yield in treated plots was not different from the weedy control. The results demonstrated the potential for postemergence applications of BSM films, which increase application timing flexibility for growers. Further research is needed to explore the effects of BSM films on soil properties and crop physiology and yield

Local Conditions, Not Regional Gradients, Drive Demographic Variation of Giant Ragweed (Ambrosia trifida) and Common Sunflower (Helianthus annuus) Across Northern U.S. Maize Belt

Knowledge of environmental factors influencing demography of weed species will improve understanding of current and future weed invasions. The objective of this study was to quantify regional-scale variation in vital rates of giant ragweed and common sunflower. To accomplish this objective, a common field experiment was conducted across seven sites between 2006 and 2008 throughout the north central U.S. maize belt. Demographic parameters of both weed species were measured in intra- and interspecific competitive environments, and environmental data were collected within site-years. Site was the strongest predictor of belowground vital rates (summer and winter seed survival and seedling recruitment), indicating sensitivity to local abiotic conditions. However, biotic factors influenced aboveground vital rates (seedling survival and fecundity). Partial least squares regression (PLSR) indicated that demography of both species was most strongly influenced by thermal time and precipitation. The first PLSR components, both characterized by thermal time, explained 63.2% and 77.0% of variation in the demography of giant ragweed and common sunflower, respectively; the second PLSR components, both characterized by precipitation, explained 18.3% and 8.5% of variation, respectively. The influence of temperature and precipitation is important in understanding the population dynamics and potential distribution of these species in response to climate change

Sequencing of \u3ci\u3eAspergillus nidulans\u3c/i\u3e and comparative analysis with \u3ci\u3eA. fumigatus\u3c/i\u3e and \u3ci\u3eA. oryzae\u3c/i\u3e

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso, and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation. Document includes all supplementary information (820 pages). Supplementary files are also attached below as Related files. THERE IS NO SUPPLEMENTARY FILE #7. PDF file size (with supplementary files included) is 10 Mbytes. An optimized version of the ARTICLE ONLY is attached as a Related File and is 1.9 Mbytes

Sequencing of \u3ci\u3eAspergillus nidulans\u3c/i\u3e and comparative analysis with \u3ci\u3eA. fumigatus\u3c/i\u3e and \u3ci\u3eA. oryzae\u3c/i\u3e

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso, and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation. Document includes all supplementary information (820 pages). Supplementary files are also attached below as Related files. THERE IS NO SUPPLEMENTARY FILE #7. PDF file size (with supplementary files included) is 10 Mbytes. An optimized version of the ARTICLE ONLY is attached as a Related File and is 1.9 Mbytes

Profitability of Abrasive Weeding in Organic Grain and Vegetable Crops

Weed competition, especially within the crop row, limits the productivity and profitability of organic crop production. Abrasive weeding, a mechanical alternative to hand weeding, uses air-propelled grits to control small weed seedlings growing within the crop row. Recent research has demonstrated the successful use of abrasive weeding to reduce weed competition and increase yields in organic maize (Zea mays), tomato (Solanum lycopersicum) and green and red pepper crops (Capsicum annuum), but the profitability of this weed control tactic has not been assessed. Our objective was to determine the profitability of abrasive weeding using empirical yield data from three previously published studies, a range of crop prices and revenues, and a range of costs for wages, grit applicator ownership, tractor use, abrasive grits, and fuel. Results suggest that abrasive weeding was not profitable in organic maize production, and may reduce net income by US$223–3537 ha−1 compared with inter-row cultivation alone for weed control. The cost of abrasive weeding in maize was largely dependent on the cost of abrasive grits and the cost to own a four-row grit applicator (US$736–2105 yr−1). However, abrasive weeding was less expensive than hand weeding, especially as the scale of production increased. Abrasive weeding was profitable in tomato and pepper crops and increased net mean income by US$12,251–33,265 ha−1. However, abrasive weeding was not 100% effective and hand weeding for weed-free conditions was always the most profitable approach to in-row weed management in vegetable crops. The profit potential of the hand-weeded, weed-free treatments demonstrates the importance of weed control in high-value specialty crops–even those grown in plastic mulch film. Despite the profit potential for hand weeding observed here, labor is increasingly difficult to source, retain and afford, and abrasive weeding offers a mechanical alternative with 66% less labor required. Further research is needed to improve the efficacy of abrasive weeding and to reduce the cost of abrasive grits and application

First-Season Crop Yield Response to Organic Soil Amendments: A Meta-Analysis

Organic soil amendments are increasingly promoted as a sustainable alternative to synthetic fertilizers and as a tool for building soil quality through improved chemical, physical, and biological properties. However, short-term yield response to organic amendments is highly variable. A meta-analysis of 53 studies was conducted to (i) develop a global estimate of first-season crop yield response to organic amendments, and (ii) determine the effect of crop type, amendment characteristics, soil properties, cultural practices, and climate on the magnitude of this yield response. Yield response ratios were calculated (organic amendment yield compared to a non-fertilized control) and differences among groups were determined using 95% bootstrap confidence intervals (CI). Across all studies, crop yield increased 43±7% (95% CI) in the first-season after an organic amendment. Yield response was greatest for leafy crops (71±26% increase) and lowest for root/tuber/bulb crops (29±10% increase). Poultry manure/compost was the most commonly used amendment and provided a yield increase of 76±21%. In contrast, plant-based amendments increased yield by only 27±9%. Amendment application rate alone was not an effective predictor of yield response, and there were not enough studies available to explore the possible interaction between amendment type and rate. Yield benefits of organic amendments were muted in soils with high organic matter and in arid climates. These results help identify options for maximizing the agronomic value of organic amendments, and suggest research is needed to improve agronomic efficiency of amendments in arid regions with poor soil quality