The Deep Subsurface Biosphere in Igneous Ocean Crust: Frontier Habitats for Microbiological Exploration

We discuss ridge flank environments in the ocean crust as habitats for subseafloor microbial life. Oceanic ridge flanks, areas far from the magmatic and tectonic influence of seafloor spreading, comprise one of the largest and least explored microbial habitats on the planet. We describe the nature of selected ridge flank crustal environments, and present a framework for delineating a continuum of conditions and processes that are likely to be important for defining subseafloor microbial "provinces." The basis for this framework is three governing conditions that help to determine the nature of subseafloor biomes: crustal age, extent of fluid flow, and thermal state. We present a brief overview of subseafloor conditions, within the context of these three characteristics, for five field sites where microbial studies have been done, are underway, or have been proposed. Technical challenges remain and likely will limit progress in studies of microbial ridge flank ecosystems, which is why it is vital to select and design future studies so as to leverage as much general understanding as possible from work focused at a small number of sites. A characterization framework such that as presented in this paper, perhaps including alternative or additional physical or chemical characteristics, is essential for achieving the greatest benefit from multidisciplinary microbial investigations of oceanic ridge flanks

Germanium in mid-ocean ridge flank hydrothermal fluids

We present concentrations of germanium and silicon in sediment pore waters, basaltic formation fluids, and bulk sediment from three ridge flank hydrothermal systems (RFHS). Basaltic formation fluids from warm (>30°C) RFHS have much higher Ge concentrations and Ge:Si molar ratios than overlying sediment pore waters, requiring seawater-basalt reactions to dominate Ge concentrations in basaltic formation fluids. In contrast to warm RFHS, cool (~20°C) RFHS have similar Ge concentrations in basal sediment pore waters and underlying basaltic formation fluids, implying that there is little net exchange between these two fluid reservoirs. Despite this low net exchange, Ge:Si molar ratios in basaltic formation fluids are elevated compared to seawater and overlying sediment pore waters, implying that seawater-basalt reactions must influence Ge and Si cycling. Such seawater-basalt reactions are likely associated with secondary clay formation because increases in Ge concentration scale with Mg loss from basaltic formation fluids. Processes that control Ge cycling in cold (3–10°C) RFHS are poorly constrained because our data are restricted to sediment pore waters that have been overprinted by diagenetic reactions and possibly sampling artifacts. Although net Ge fluxes from RFHS prevail over a wide temperature range, a refined estimate for the global RFHS Ge flux is currently not possible without data from cold RFHS springs or basaltic formation fluids because cold RFHS transport most of the convective heat and crustal fluid to the oceans.Keywords: ridge flank, germanium, hydrothermal, geochemical cycles, sediment, silic

Spatial variation of subduction zone fluids during progressive subduction: Insights from Serpentinite Mud Volcanoes

Geological processes at subduction zones control seismicity, plutonism and volcanism, and geochemical cycling between the oceans, crust, and mantle. The down-going plate experiences metamorphism, and the associated dehydration and fluid flow alters the physical properties of the plate interface and mantle wedge, as well as controlling the composition of material descending into the mantle. Any direct study of slab evolution during subduction is inhibited by the prohibitive depths at which these processes occur. To examine these processes we use serpentinite mud volcanoes in the Mariana forearc, that permit sampling of serpentinite materials and their pore waters that ascend from the subduction channel. We present new pore water chemical data from the summit and flanks of three serpentinite mud volcanoes that were drilled during International Ocean Discovery Program Expedition 366 which are reflective of reactions within the crust and mantle during the early, shallow (<20 km) stages of subduction. We show, via thermodynamic modelling, that our new data on the evolution of pore water chemical compositions reflect mineralogical characteristics of a predominately basaltic source from the downgoing Pacific Plate. However, a component from sedimentary sources is likely, especially for those mud volcanoes near the trench. Other potential slab-derived constituents, such as lithospheric serpentinite, carbonate-rich sediments, or seamount basalts with an intraplate geochemical character, are not required to explain our results. Our results indicate that with progressive subduction the lawsonite-epidote mineral transformation boundary at ∼250 °C may help drive slab carbonate destabilisation, despite its apparent thermodynamic stability at such temperatures and projected pressures (∼300 °C and ∼0.6 GPa). New dissolved gas data also point to primary thermodynamic controls over methane/ethane production within the subduction channel as depths-to-slab increase. Our findings provide direct evidence for the progressive mineralogical and chemical evolution of a subducting oceanic plate, which liberates a progressively evolving fluid phase into the subduction channel

Oceanic phosphorus imbalance : magnitude of the mid-ocean ridge flank hydrothermal sink

We present a new estimate for the crustal phosphorous sink that results from reactions among seawater, basalt, and sediment blanketing low temperature mid-ocean ridge flank hydrothermal systems. New estimates for global hydrothermal power output, sediment thickness, and the dissolved phosphate concentrations in basement formation fluids indicate that fluid flow through ridge flanks removes 2.8 x 10¹⁰ mol P yr⁻¹. This value is larger (130%) than the riverine dissolved flux of inorganic phosphate and is as much as 35% of the sedimentary P sink. The concordant seawater flux (2.1 x 10¹⁶ kg yr⁻¹) is 65% of the riverine fluid flux and circulates a fluid volume equivalent to the entire ocean in about 70,000 yr. Additional sampling of seafloor springs is required to further constrain the range of calculated phosphate fluxes; nevertheless the modern phosphorus budget is clearly unbalanced with total sinks outpacing sources.Keywords: Ocean Drilling Program, geochemical cycles, ridge flank, phosphate, hydrotherma

Data report : trace element, Sr isotope, and Ge/SI composition of fluid and sediments in ridge-flank low-temperature hydrothermal environments

The data presented in this report demonstrate significant improvements in the ability to constrain trace element and Sr isotopic concentrations in sediments overlying ridge-flank hydrothermal systems. Improved sampling methods orchestrated by the Integrated Ocean Drilling Program (i.e., advanced piston coring and anoxic sample processing) enabled the collection of reactive pore water species with minimal alteration and sampling artifacts. Improved methods of high-resolution inductively coupled plasma–mass spectrometry trace element analysis, including the use of the 8-hydroxyquinoline functional group to extract and preconcentrate rare earth elements and other trace metals, were used to compile a data set of 28 trace element concentrations and ⁸⁷Sr/⁸⁶Sr ratios. From this extensive data set, we were able to increase the current understanding of how redox-reactive species respond to diagenic processes. Near-basement trends were used in combination with the known composition of hydrothermal fluids that exit Baby Bare Springs to asses our ability to predict basement fluid compositions using sediment pore water profiles collected by deep-sea drilling. The results show that prediction of basement fluid composition is possible for many trace elements, provided the near-basement concentration gradients are minimal. In order to place the Ge/Si systematics in a broader context, pore water and borehole fluid Ge and Si data are presented from additional sites across the Juan de Fuca Ridge flank and from two additional ridge-flank settings. These data show that Ge concentrations and Ge/Si ratios are much higher in the basement fluids than in the basal sediments because of increased mobilization of Ge relative to Si within the basement hydrothermal reservoir. Solid-phase sediment data are presented, highlighted by the occurrence of Mn- and carbonate-rich layers

Hydrothermal fluid circulation through the sediment of Crater Lake, Oregon: Pore water and heat flow constraints

We present evidence for pore water flow through the sediment of Crater Lake, Oregon based on systematic variations in pore water chemical compositions and thermal gradients. Pore water was extracted from sediment by centrifugation and diffusive exchange using a gravity corer deployed from a surface vessel and a box corer and peepers deployed from the submersible Deep Rover in a known geologic context. Depth profiles of sediment temperature were measured using two probes deployed from the submersible. One probe was connected to the submersible whereas the other was self‐contained and deployed for 7 days. On the basis of measured and calculated depth profiles of pore water Na, Ca, Mg, K, Li, and temperature, we show that pore water upwells in zones of focused upflow at speeds of meters to hundreds of meters per year. These zones of focused flow are patchy and usually cover several square meters to hundreds of square meters and are marked by iron‐manganese‐rich crusts, bacterial mats, and saline pools. In contrast, most of the lake floor consists of sediment derived from the caldera walls and has a low heat flow with pore water velocities of millimeters per year. The chemical composition of the pore water that upwells through the sampled section of the sediment column differs from core to core. This difference results from mixing a hydrothermal fluid in igneous basement below the lake with lake water before the final ascent through the sediment column. Elemental ratios of this thermally and chemically altered fluid in basement match those calculated based on mass balance considerations. Calculation of mass balance and geothermometry constrain the temperature in basement and ultimately the power output, which is about 30 MW. This power output is in agreement with two other estimates that were calculated using temperature data from the water column and measurements of sediment heat flow

Oceanic molybdenum isotope fractionation: Diagenesis and hydrothermal ridge-flank alteration

Isotopic analyses of dissolved molybdenum are presented for sediment pore waters from a reducing sedimentary basin and for fluids from a low-temperature ridge flank hydrothermal system. δ⁹⁸/⁹⁵Mo in these fluids range from 0.8 to 3.5%₀ (relative to a laboratory standard), demonstrating that marine sedimentary reactions significantly fractionate Mo isotopes. Within the upper 3 cm of sediment, manganese oxide dissolution produces an isotopically light fluid relative to seawater (mean of four analyses = 2.1 ± 0.1%₀ versus seawater = 2.3 ± 0.1%₀). Below 6 cm depth, authigenic Mo uptake results in an isotopically heavier fluid (up to 3.5%₀) indicating that reducing sediments are likely to be a net sink for isotopically light dissolved Mo. In contrast, fluid circulation within a low-temperature ridge-flank hydrothermal system is a source of isotopically light Mo to the ocean having an end-member fluid of ~0.8%₀.Keywords: molybdenum isotopes, molybdenum, isotope geochemistry, sediment diagenesisKeywords: molybdenum isotopes, molybdenum, isotope geochemistry, sediment diagenesi

Continuous Sampling of Hydrothermal Fluids From Loihi Seamount After the 1996 Event

For at least 9 years prior to July 1996, hydrothermal fluids flowed from Pele\u27s Vents on Loihi Seamount, Hawaii. In July–August 1996 a tectonic-volcanic event occurred that destroyed Pele\u27s Vents, creating a pit crater (Pele\u27s Pit) and several sites with hydrothermal venting. In October 1996 we deployed two new continuous water samplers (OsmoSamplers) at two of these hydrothermal sites and collected fluids using traditional sampling techniques to monitor the evolution of crustal and hydrothermal conditions after the event. The samplers were recovered in September 1997, and additional discrete vent fluid samples were collected. The OsmoSampler located along the south rift at Naha Vents captured a change in composition from a low-chlorinity, high-K fluid (relative to bottom seawater) to a high-chlorinity, low-K fluid. These changes are consistent with the fluid cooling during ascent and being derived from several different sources, which include high- (\u3e330°C) and low- (330°C) into which magmatic volatiles were added. During the deployment, thermal and fluid fluxes decreased. At Naha the transport of heat and chemicals was decoupled. The chemical and thermal evolution of hydrothermal fluids after the event on Loihi is consistent with previous models based on events that have occurred along mid-ocean ridges. The event at Loihi clearly had an effect on the local hydrography; however, the integrated effect of chemical fluxes to global budgets from similar events is uncertain. Chemical fluxes from similar events may have a global impact, if ratios of chemical (e.g., CO2, Fe/Mn, Mg, sulfate, and K) to thermal anomalies greatly exceed, or are in the opposite direction to, fluxes from mid-ocean ridge hydrothermal systems