The applications of computers in biological research

Research in many fields could not be done without computers. There is often a great deal of technical data, even in the biological fields, that need to be analyzed. These data, unfortunately, previously absorbed much of every researcher's time. Now, due to the steady increase in computer technology, biological researchers are able to make incredible advances in their work without the added worries of tedious and difficult tasks such as the many mathematical calculations involved in today's research and health care

Construction safety and digital design: a review

As digital technologies become widely used in designing buildings and infrastructure, questions arise about their impacts on construction safety. This review explores relationships between construction safety and digital design practices with the aim of fostering and directing further research. It surveys state-of-the-art research on databases, virtual reality, geographic information systems, 4D CAD, building information modeling and sensing technologies, finding various digital tools for addressing safety issues in the construction phase, but few tools to support design for construction safety. It also considers a literature on safety critical, digital and design practices that raises a general concern about ‘mindlessness’ in the use of technologies, and has implications for the emerging research agenda around construction safety and digital design. Bringing these strands of literature together suggests new kinds of interventions, such as the development of tools and processes for using digital models to promote mindfulness through multi-party collaboration on safet

Qualitative Assessment of the PAX Good Behavior Game Implementation

This paper reports on a program evaluation of the PAX Good Behavior Game (GBG), an evidence-based practice intervention designed to create a nurturing environment conducive to learning in elementary schools. To evaluate and improve the PAX Good Behavior Game, a focus group was conducted at the end of the 2016-17 academic year. A total of ten teachers and school administrators from schools who implemented the PAX Good Behavior Game (PAX professionals) participated in a focus group session and provided feedback about the program. Focus group questions assessed four program objectives: (1) environmental change, (2) personal well-being and stress levels, (3) engagement with parents, and (4) networking with other PAX professionals. Results indicated that the PAX GBG decreased problematic classroom behaviors, provided more instructional time for teachers, and generated public interest of the program in the home and community

Carbon Cycle Science Data and Services at the Goddard Earth Sciences Data Information and Services Center (GES DISC)

The Goddard Earth Sciences Data Information and Services Center (GES DISC) archives and distributes a number of observational and model carbon cycle science data sets. We also provide services that facilitate data discovery, intercomparison, and visualization of these heterogeneous datasets for both research and applications users, such as subsetting, format conversion, How-To documentation, and the Help Desk

CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method.

Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed. To generate genetically modified animals, CRISPR/Cas9-mediated genome editing is typically accomplished by microinjection of a mixture of Cas9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes. However, sgRNAs used for this approach require manipulation via molecular cloning as well as in vitro transcription. Beyond these complexities, most mutants obtained with this traditional approach are genetically mosaic, yielding several types of cells with different genetic mutations. Recently, a growing body of studies has utilized commercially available Cas9 protein together with sgRNA and a targeting construct to introduce desired mutations. Here, we report a cloning-free method to target the mouse genome by pronuclear injection of a commercial Cas9 protein:crRNA:tracrRNA:single-strand oligodeoxynucleotide (ssODN) complex into mouse zygotes. As illustration of this method, we report the successful generation of global gene-knockout, single-amino-acid-substituted, as well as floxed mice that can be used for conditional gene-targeting. These models were produced with high efficiency to generate non-mosaic mutant mice with a high germline transmission rate

The Solar Neighborhood XLII. Parallax Results from the CTIOPI 0.9-m Program --- Identifying New Nearby Subdwarfs Using Tangential Velocities and Locations on the H-R Diagram

Parallaxes, proper motions, and optical photometry are presented for 51 systems made up 37 cool subdwarf and 14 additional high proper motion systems. Thirty-seven systems have parallaxes reported for the first time, 15 of which have proper motions of at least 1"/yr. The sample includes 22 newly identified cool subdwarfs within 100 pc, of which three are within 25 pc, and an additional five subdwarfs from 100-160 pc. Two systems --- LSR 1610-0040 AB and LHS 440 AB --- are close binaries exhibiting clear astrometric perturbations that will ultimately provide important masses for cool subdwarfs. We use the accurate parallaxes and proper motions provided here, combined with additional data from our program and others to determine that effectively all nearby stars with tangential velocities greater than 200 km s$^{-1}$ are subdwarfs. We compare a sample of 167 confirmed cool subdwarfs to nearby main sequence dwarfs and Pleiades members on an observational Hertzsprung-Russell diagram using $M_V$ vs.~$(V-K_{s})$ to map trends of age and metallicity. We find that subdwarfs are clearly separated for spectral types K5--M5, indicating that the low metallicities of subdwarfs set them apart in the H-R diagram for $(V-K_{s})$ = 3--6. We then apply the tangential velocity cutoff and the subdwarf region of the H-R diagram to stars with parallaxes from {\it Gaia} Data Release 1 and the MEarth Project to identify a total of 29 new nearby subdwarf candidates that fall clearly below the main sequence.Comment: accepted for publication in Astronomical Journa