20,684 research outputs found
Improved calorimeter provides accurate thermal measurements of space batteries
Isothermal continuous flow calorimeter measures the thermal characteristics of space batteries undergoing typical orbital cycling. This is 28 times as sensitive as calorimeters previously used
Digital multiplexed mRNA analysis of functionally important genes in single human oocytes and correlation of changes in transcript levels with oocyte protein expression
Objective
To investigate functionally important transcripts in single human oocytes with the use of NanoString technology and determine whether observed differences are biologically meaningful.
Design
Analysis of human oocytes with the use of NanoString and immunoblotting.
Setting
University-affiliated reproductive medicine unit.
Patients
Women undergoing in vitro fertilization.
Intervention
Human oocytes were analyzed with the use of NanoString or immunoblotting.
Main Outcome Measures
The abundance of transcripts for ten functionally important genes—AURKA, AURKC, BUB1, BUB1B (encoding BubR1), CDK1, CHEK1, FYN, MOS, MAP2K1, and WEE2—and six functionally dispensable genes were analyzed with the use of NanoString. BubR1 protein levels in oocytes from younger and older women were compared with the use of immunoblotting.
Result(s)
All ten functional genes but none of the six dispensable genes were detectable with the use of NanoString in single oocytes. There was 3- to 5-fold variation in BUB1, BUB1B, and CDK1 transcript abundance among individual oocytes from a single patient. Transcripts for these three genes—all players within the spindle assembly checkpoint surveillance mechanism for preventing aneuploidy—were reduced in the same oocyte from an older patient. Mean BUB1B transcripts were reduced by 1.5-fold with aging and associated with marked reductions in BubR1 protein levels.
Conclusion(s)
The abundance of functionally important transcripts exhibit marked oocyte-to-oocyte heterogeneity to a degree that is sufficient to affect protein expression. Observed variations in transcript abundance are therefore likely to be biologically meaningful, especially if multiple genes within the same pathway are simultaneously affected
Cereal Leaf Beetle (Coleoptera: Chrysomelidae) Influence of Seeding Rate of Oats on Populations
In field and greenhouse studies, more cereal leaf beetle [Oulema melanopus (Lin- naeus)] eggs and larvae were found per unit area on spring oats, Avena sativa L., planted either at intermediate (54 kg/ha) or high (136 kg/ha) seeding rates, than when planted at a lower seeding rate (14 kg/ha). However, there were fewer eggs and larvae per stem in plantings of the high or intermediate rates than in those of the lower rate. Oats should not be planted at less than the recommended rates in beetle-infested areas
Heater improves cold-temperature capacity of silver-cadmium batteries
Eight heaters are included in 14-cell package to provide 14-Vdc. Each heater is 11-ohm self-adhesive strip placed across broad face of each pair of cells. They are installed before cells are wired. Heaters are in series and are connected through pair of redundant thermostats
Research into fundamental phenomena associated with spacecraft electrochemical devices - calorimetry of nickel-cadmium cells Progress report, 1 Oct. 1966 - 31 Dec. 1966
Cycling, charge efficiency, and transducer studies with nickel-cadmium cell for spacecraft power supply system
Urban Regeneration of Industrial Areas: Affordable Housing for Low Income Populations in Cities
The UK-China Sustainable Development Dialogue (SDD) is a partnership between the UK and Chinese Governments to promote collaboration and good practice on sustainable development. It is framed by a 2004 joint Prime Ministerial declaration and was formally established in 2005 by an agreement signed by UK Deputy Prime Minister John Prescott and State Councillor Tang. The SDD was founded on the recognition that, in an interdependent world, international co-operation is needed to ensure that learning is shared and efforts are made collectively towards achieving common goals. This report is the 13th and Final Paper in the SDD (Urban Strand. It draws together the core conclusions from the previous twelve papers and offers recommendations for taking the dialogue forward
Categorification of quantum symmetric pairs I
We categorify a coideal subalgebra of the quantum group of
by introducing a -category \`a la
Khovanov-Lauda-Rouquier, and show that self-dual indecomposable -morphisms
categorify the canonical basis of this algebra. This allows us to define a
categorical action of this coideal algebra on the categories of modules over
cohomology rings of partial flag varieties and on the BGG category
of type B/C.Comment: final version, to appear in Quantum Topolog
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