La plataforma iTecSoft: Un caso de colaboración inter-organizativa 2.0.

El artículo describe la metodología utilizada para abordar el problema de la colaboración intra- e inter-organizativa mediante la descripción del caso específico del proyecto ITECBAN. En este caso, la colaboración se describe en los términos de la organización virtual y el problema se afronta bajo parámetros de flexibilidad a la hora de definir y gestionar esa organización virtual, formada por equipos de trabajo y profesionales geográfica y organizativamente dispersos. La solución planteada, la plataforma Itecsoft, se apoya en una arquitectura abierta de colaboración y una serie de componente de middleware y servicios que acaban tomando sustanciándose en un conjunto de herramientas de código libre en su mayoría provenientes de proyectos y comunidades que han surgido o se han reactivado al calor del fenómeno “dos-punto-cero”. Las principales aportaciones de la solución propuesta son la resolución del problema de la identidad en el entorno distribuido y descentralizado de colaboración de ITECBAN, junto con la selección, evaluación e integración de las herramientas de colaboración dentro de esa arquitectura abierta

Meaning of reflective practice in the acquisition and transfer of communication skills in nursing

La práctica reflexiva constituye un método educativo de cambio destinado a desarrollar las competencias profesionales del estudiante de enfermería. El proceso parte de la capacidad autocrítica, del reconocimiento de las potencialidades y limitaciones, del fomento de la responsabilidad y del crecimiento personal. Sin embargo, los cambios obtenidos tras ese proceso formativo pueden no mantenerse en la etapa profesional. El objetivo principal de este estudio fue explorar el significado que las enfermeras atribuían al proceso educativo, a lo largo de su formación universitaria, de adquisición de competencias relacionales mediante la práctica reflexiva y a su mantenimiento posterior. Desde una aproximación fenomenológica se entrevistó a dieciséis enfermeras que habían adquirido competencias relacionales a través de la práctica reflexiva. Del análisis de los datos resultó un dominio central que se denominó «espíritu del prácticum» y cinco núcleos temáticos: aprendizaje profesional, prejuicios y dificultades personales, características y desarrollo de la profesión, crecimiento personal y profesional, y evolución hacia la profesionalidad. La transformación y el cambio experimentados durante el prácticum se mantuvieron en el tiempo y se transfirieron a la práctica profesional en ámbitos de enfermería distintos a la salud mental. Junto con la voluntad de transferir las competencias adquiridas, los resultados revelaron dificultades asociadas a las condiciones laborales y la necesidad de un mayor soporte externo y formación continuada. Si bien este estudio evidencia la importancia de la práctica reflexiva como método educativo para la adquisición de competencias profesionales, se considera necesaria una mayor indagación sobre todo respecto a su uso en etapas profesionales de postgrado

BRCA1 mutations in high-grade serous ovarian cancer are associated with proteomic changes in DNA repair, splicing, transcription regulation and signaling

Ovarian cancer; ProteomicsCáncer de ovarios: ProteómicaCàncer d'ovaris; ProteòmicaDespite recent advances in the management of BRCA1 mutated high-grade serous ovarian cancer (HGSC), the physiology of these tumors remains poorly understood. Here we provide a comprehensive molecular understanding of the signaling processes that drive HGSC pathogenesis with the addition of valuable ubiquitination profiling, and their dependency on BRCA1 mutation-state directly in patient-derived tissues. Using a multilayered proteomic approach, we show the tight coordination between the ubiquitination and phosphorylation regulatory layers and their role in key cellular processes related to BRCA1-dependent HGSC pathogenesis. In addition, we identify key bridging proteins, kinase activity, and post-translational modifications responsible for molding distinct cancer phenotypes, thus providing new opportunities for therapeutic intervention, and ultimately advance towards a more personalized patient care.This work was supported by the PhD4MD collaborative research program between the Vall d’Hebron Research Institute (VHIR) and the Centre for Genomic Regulation (CRG). The CRG/UPF Proteomics Unit is part of the Spanish Infrastructure for Omics Technologies (ICTS OmicsTech) and it is a member of the ProteoRed PRB3 consortium which is supported by grant PT17/0019 of the PE I+D+i 2013-2016 from the Instituto de Salud Carlos III (ISCIII) and ERDF. We acknowledge support from the Spanish Ministry of Science, Innovation and Universities, (CTQ2016-80364-P and “Centro de Excelencia Severo Ochoa 2013-2017”, SEV-2012-0208), and “Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya” (2017SGR595 and 2017SGR1661). This project has also received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 823839 (EPIC-XS). It has also been supported by grants from the Instituto Carlos III (PI15/00238, PI18/01017, PI21/00977), the Miguel Servet Program (CP13/00158 and CPII18/00027) and the Ministerio de Economía y Competitividad y Fondos FEDER (RTC-2015-3821-1). The authors are grateful to the team members of the Proteomics Unit at the Centre for Genomic Regulation, the Biomedical Research Group in Gynecology at the Vall d’Hebron Institute, the Gynecological Oncology Unit at the Vall d’Hebron Hospital and the Biomedical Research Group in Urology at the Vall d’Hebron Institute for their assistance

A combination of molecular and clinical parameters provides a new strategy for high-grade serous ovarian cancer patient management

Biomarker; Prediction; ProteomicsBiomarcador; Predicción; ProteómicaBiomarcador; Predicció; ProteòmicaBackground High-grade serous carcinoma (HGSC) is the most common and deadly subtype of ovarian cancer. Although most patients will initially respond to first-line treatment with a combination of surgery and platinum-based chemotherapy, up to a quarter will be resistant to treatment. We aimed to identify a new strategy to improve HGSC patient management at the time of cancer diagnosis (HGSC-1LTR). Methods A total of 109 ready-available formalin-fixed paraffin-embedded HGSC tissues obtained at the time of HGSC diagnosis were selected for proteomic analysis. Clinical data, treatment approach and outcomes were collected for all patients. An initial discovery cohort (n = 21) were divided into chemoresistant and chemosensitive groups and evaluated using discovery mass-spectrometry (MS)-based proteomics. Proteins showing differential abundance between groups were verified in a verification cohort (n = 88) using targeted MS-based proteomics. A logistic regression model was used to select those proteins able to correctly classify patients into chemoresistant and chemosensitive. The classification performance of the protein and clinical data combinations were assessed through the generation of receiver operating characteristic (ROC) curves. Results Using the HGSC-1LTR strategy we have identified a molecular signature (TKT, LAMC1 and FUCO) that combined with ready available clinical data (patients’ age, menopausal status, serum CA125 levels, and treatment approach) is able to predict patient response to first-line treatment with an AUC: 0.82 (95% CI 0.72–0.92). Conclusions We have established a new strategy that combines molecular and clinical parameters to predict the response to first-line treatment in HGSC patients (HGSC-1LTR). This strategy can allow the identification of chemoresistance at the time of diagnosis providing the optimization of therapeutic decision making and the evaluation of alternative treatment strategies. Thus, advancing towards the improvement of patient outcome and the individualization of HGSC patients’ care.This work was supported by the PhD4MD collaborative research program between the Vall d’Hebron Research Institute (VHIR) and the Centre for Genomic Regulation (CRG). It has been supported by grants from the Instituto Carlos III (PI18/01017), the Miguel Servet Program (CPII18/00027) and the Ministerio de Economía y Competitividad y Fondos FEDER (RTC-2015-3821-1 to AS and CTQ2016-80364-P to ES). This project has also received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 823839 (EPIC-XS).The CRG/UPF Proteomics Unit is part of the Spanish Infrastructure for Omics Technologies (ICTS OmicsTech) and it is supported by “Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya” (2017SGR595 and 2017SGR1661). We also acknowledge support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme / Generalitat de Catalunya

A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

Tuberculosis, one of the oldest diseases affecting human beings, is still considered as a world public health problem by the World Health Organization. Therefore, there is a need for new and more powerful analytical methods for early illness diagnosis. With this idea in mind, the development of a High Fundamental Frequency (HFF) piezoelectric immunosensor for the sensitive detection of tuberculosis was undertaken. A 38 kDa protein secreted by Mycobacterium tuberculosis was first selected as the target biomarker. Then, specific monoclonal antibodies (MAbs) were obtained. Myc-31 MAb, which showed the highest affinity to the analyte, was employed to set up a reference enzyme-linked immunosorbent assay (ELISA) with a limit of detection of 14 ng mL-1 of 38 kDa antigen. For the development of the HFF piezoelectric immunosensor, 100 MHz quartz crystals were used as transducer elements. The gold electrode surface was functionalized by covalent immobilization of the target biomarker through mixed self-assembled monolayers (mSAM) of carboxylic alkane thiols. A competitive immunoassay based on Myc-31 MAb was integrated with the transducer as sensing bio-recognition event. Reliable assay signals were obtained using low concentrations of antigen for functionalization and MAb for the competitive immunoassay. Under optimized conditions, the HFF immunosensor calibration curve for 38 kDa determination showed a limit of detection as low as 11 ng mL-1 of the biomarker. The high detectability attained by this immunosensor, in the picomolar range, makes it a promising tool for the easy, direct and sensitive detection of the tuberculosis biomarker in biological fluids such as sputum.This study was supported by COLCIENCIAS (Colombia), Project no. 13335212865) and by AWSensors (Valencia, Spain). All of the authors: A. Montoya, C. March, Y.J. Montagut, M.J. Moreno, J.J. Manclus, A. Arnau, Y. Jimenez, M. Jaramillo, P. A. Marin and R.A. Torres declare that they have no conflict of interest.Montoya Baides, Á.; March Iborra, MDC.; Montagut Ferizzola, YJ.; Moreno Tamarit, MJ.; Manclus Ciscar, JJ.; Arnau Vives, A.; Jiménez Jiménez, Y.... (2017). A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection. Current Topics in Medicinal Chemistry. 17(14):1623-1630. https://doi.org/10.2174/1568026617666161104105210S16231630171

Propuesta de mejora de la asignatura TFG del Grado en Nutrición Humana y Diétetica. Implantación de la prueba ECOE

Depto. de Farmacología y ToxicologíaFac. de MedicinaFALSEsubmitte

Proposta sobre l’ordenació de la cirurgia bariàtrica en població adulta als hospitals públics de Catalunya

Cirugía bariátrica; Hospitales públicos; AdultosBariatric surgery; Public hospitals; AdultsCirurgia bariàtrica; Hospitals públics; AdultsL'objectiu és definir les indicacions de cirurgia bariàtrica en població adulta i disminuir la variabilitat entre centres hospitalaris; analitzar i definir els diferents nivells quirúrgics segons la complexitat de la cirurgia bariàtrica en població adulta; definir el seguiment a curt/mitjà i llarg termini de la postcirurgia bariàtrica en població adulta i establir els criteris i requeriments mínims dels centres hospitalaris per dur a terme cirurgia bariàtrica en població adulta. L’àmbit d’aplicació del consens és el Sistema sanitari integral d’utilització pública de Catalunya (SISCAT).El objetivo es definir las indicaciones de cirugía bariátrica en población adulta y disminuir la variabilidad entre centros hospitalarios; analizar y definir los diferentes niveles quirúrgicos segundos la complejidad de la cirugía bariátrica en población adulta; definir el seguimiento a corto/medio y largo plazo de la postcirugía bariátrica en población adulta y establecer los criterios y requerimientos mínimos de los centros hospitalarios para llevar a cabo cirugía bariátrica en población adulta. El ámbito de aplicación del consenso es el Sistema sanitario integral de utilización pública de Cataluña (SISCAT).The aim is to define the indications for bariatric surgery in the adult population and to decrease the variability between hospitals; analyze and define the different surgical levels according to the complexity of bariatric surgery in the adult population; define the short / medium and long-term follow-up of bariatric surgery in the adult population and establish the minimum criteria and requirements for hospitals to carry out bariatric surgery in the adult population. The scope of the consensus is the Comprehensive Health System for Public Use in Catalonia (SISCAT)

Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)

© 2021 The Author(s).The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients.This project was supported by the AECC (GC16173697BIGA); ISCIII (PI19/01828) co-funded by ERDF/ESF "A way to make Europe"/ "Investing in your future", CERCA/Generalitat de Catalunya SGR 2017 288 (GRC)/ “La Caixa” P. Barba was supported by the Instituto de Salud Carlos III FIS16/01433 and PERIS 2018-2020 from Generalitat de Catalunya (BDNS357800)

Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)

The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients