Gestão de documentos em arquivos de museus: descobrindo o acervo arquivístico da Oficina de Criatividade / HPSP

O referido trabalho analisa o acervo documental da Oficina de Criatividade, espaço do Hospital Psiquiátrico São Pedro localizado em Porto Alegre/RS que se dedica a realizar atividades no âmbito da reabilitação psicossocial utilizando as artes plásticas como mote para estabelecer outras relações e sentidos com seus frequentadores desde 1990, ano de criação da Oficina de Criatividade. Para isso foi realizado um estudo de caso aplicado, qualitativo. A análise foi realizada por meio de visitas a Oficina e com o reconhecimento dos documentos do acervo assim como com conversas com profissionais que atuam no local. O resultado desse trabalho possibilitou a realização de um diagnóstico arquivístico com ênfase às funções de classificação e descrição sendo contempladas com uma proposta para organização do acervo do recém implantado Museu Estadual Oficina de Criatividade (MEOC HPSP). A proposta de organização documental não tem o objetivo de sanar todas as necessidades do acervo que apresenta algumas falhas de gestão desde a concepção dos documentos, mas um acréscimo de esforço ao trabalho de quem cotidianamente busca preservar a história e a memória deste espaço.This work analyzes the documentary collection of the Oficina de Criatividade, a space of the São Pedro Psychiatric Hospital located in Porto Alegre/RS that is dedicated to carrying out activities in the scope of psychosocial rehabilitation using the visual arts as a motto to establish other relationships and meanings with its visitors. since 1990, the year of creation of the Oficina de Criatividade. For this, an applied, qualitative case study was carried out. The analysis was carried out through visits to the Workshop and with the recognition of the documents in the collection, as well as conversations with professionals who work in the place. The result of this work made it possible to carry out an archival diagnosis with emphasis on the functions of classification and description being contemplated with a proposal for organizing the collection of the recently implemented State Museum Oficina de Criatividade (MEOC-HPSP). The proposal for document organization is not intended to solve all the needs of the collection, which has some management failures since the conception of the documents, but an increase in effort to the work of those who daily seek to preserve the history and memory of this space

Genome sequence of Enterococcus mundtii EM01, isolated from Bombyx mori midgut and responsible for flacherie disease in silkworms reared on an artificial diet

The whole genome sequence of Enterococcus mundtii strain EM01 is reported here. The isolate proved to be the cause of flacherie in Bombyx mori. To date, the genomes of 11 other E. mundtii strains have been sequenced. EM01 is the only strain that displayed active pathological effects on its associated animal species

Alterações numéricas e volumétricas das células de Leydig no envelhecimento de ratos

Orientador: Prof. Dr. Rogério de FragaCoorientador: Prof. Dr. Fernando LorenziniDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Clínica Cirúrgica. Defesa: Curitiba, 17/07/2017Referências: p. 47-49Resumo: Introdução: As células de Leydig são responsáveis pela produção de testosterona e sofrem os efeitos deletérios do envelhecimento. Com o avançar da idade, ocorre uma diminuição na esteroidogênese por alterações celulares ainda pouco conhecidas. Objetivos: Analisar os efeitos do envelhecimento nos ratos sobre o volume nuclear, citoplasmático, volume total e o número absoluto das células de Leydig. Material e métodos: Selecionados 72 ratos machos Wistar, aleatoriamente divididos em seis subgrupos com 12 roedores cada. Os animais foram submetidos à orquietomia direita aos 3, 6, 9, 12, 18 e 24 meses de vida. Foram avaliados o peso e o volume do testículo direito. Realizado estudo estereológico das células de Leydig com medidas do volume nuclear, citoplasmático, volume total e contabilizado o número destas células. Resultados: O peso e o volume dos testículos apresentaram reduções que acompanharam o desenvolvimento corporal dos ratos. O volume nuclear apresentou redução no subgrupo de ratos com 24 meses de vida. O volume citoplasmático e o volume total das células de Leydig apresentaram reduções significativas nos subgrupos de ratos idosos (18 e 24 meses). Não houve redução na contagem das células de Leydig nos subgrupos. Conclusão: O envelhecimento nos ratos causou alterações nas células de Leydig, caracterizadas pela redução do volume nuclear, citoplasmático e total. Não houve variação numérica destas células. Palavras-chave: Envelhecimento. Ratos. Testículo. Células de Leydig. Estereologia. Hipogonadismo. Testosterona.Abstract: Introduction: Leydig cells are responsible for producing testosterone and suffer the deleterious effects of time. With aging, there is a decrease in testosterone production due to cellular changes that remain unknown. Purpose: To analyze the effects of aging in rats on the nuclear volume, cytoplasmic volume, and total volume of Leydig cells, as well as their number. Methods: Seventy-two Wistar rats were divided into six subgroups of 12 rats, which underwent right orchiectomy at 3, 6, 9, 12, 18, and 24 months of age. The weight and volume of the resected testicles were assessed. A stereological study of Leydig cells was conducted, which included measurements of cell number and nuclear, cytoplasmic, and total cell volumes. Results: The weight and volume of the resected testicles showed reductions with age. Only the subgroup composed of 24-month old rats showed a decrease in the nuclear volume of Leydig cells. Significant reductions in the cytoplasmic volume and total volume of Leydig cells were observed in 18- and 24-month old rats. The number of Leydig cells did not vary significantly with age. Conclusion: Aging in rats resulted in reduction of the nuclear, cytoplasmic, and total cell volumes of Leydig cells. There was no change in the total number of these cells during aging. Keywords: Aging. Rats. Leydig Cells. Testicle. Hypogonadism. Testosterone. Stereolog

p14 expression differences in ovarian benign, borderline and malignant epithelial tumors

Background: Abnormalities in tumor suppressors p14, p16 and p53 are reported in several human cancers. In ovarian epithelial carcinogenesis, p16 and p53 show higher immunohistochemical staining frequencies in malignant tumors and are associated with poor prognoses. p14 was only analyzed in carcinomas, with conflicting results. There are no reports on its expression in benign and borderline tumors. This study aims to determine p14, p16 and p53 expression frequencies in ovarian benign, borderline and malignant tumors and their associations with clinical parameters. Methods: A cross-sectional study utilizing immunohistochemistry was performed on paraffin-embedded ovarian epithelial tumor samples. Clinical data were collected from medical records. Fisher’s exact test and the Bonferroni correction were performed for frequency associations. Survival comparisons utilized Kaplan-Meier and log rank testing. Associations were considered significant when p < 0.05. Results: p14 absent expression was associated with malignant tumors (60 % positive) (p = 0.000), while 93 % and 94 % of benign and borderline tumors, respectively, were positive. p16 was positive in 94.6 % of carcinomas, 75 % of borderline and 45.7 % of benign tumors (p = 0.000). p53 negative staining was associated with benign tumors (2.9 % positive) (p = 0.016) but no difference was observed between borderline (16.7 %) and malignant tumors (29.7 %) (p = 0.560). No associations were found between expression rates, disease-free survival times or clinical variables. Carcinoma subtypes showed no difference in expression. Conclusions: This is the first description of p14 expression in benign and borderline tumors. It remains stable in benign and borderline tumors, while carcinomas show a significant absence of staining. This may indicate that p14 abnormalities occur later in carcinogenesis. p16 and p53 frequencies increase from benign to borderline and malignant tumors, similarly to previous reports, possibly reflecting the accumulation of inactive mutant protein. The small sample size may have prevented statistically significant survival analyses and clinical correlations. Future studies should investigate genetic abnormalities in p14 coding sequences and include all types of ovarian epithelial tumors. Bigger sample sizes may be needed for significant associations