169 research outputs found
THE ESTABLISHMENT OF INTENSIVE APPLE ORCHARDS IN SERBIA
Serbia at the present time grows apple on an area of 25.917 ha with an average production of 412.000 tons per year. This production is almost 2.5 fold higher than in the period of 2001-2005., which is associated with establishment of new intensive orchards, starting from 2006.Apple production was moving from the locations, typically used for traditional apple production to the regions, mostly located in the different valleys, that poses enough quantity of fresh water for drip irrigation. The new established orchards are equipped with anti-hail net preventingfruit damagesagainst hail or intensive sunlight. The most dominant cultivars are different clones of Golden Delicious, Granny Smith, Gala and Red Delicious, which are mostly grafted on M9 rootstock. Spacing between the rows is the same as in the past (3.0-3.5 m), while distance withinthe rows is significantly reduced and now is 0,5-0,9 m, which provide 3,200-6,250 trees ha-1. Tree height reaches 2.20-3.0 m. Large and well feathered nursery trees are used for planting, which provide fast returns of high investment. “Knip” nursery trees as 2-year-old trees with one-year old crown are preferred for establishing new orchards. After planting, light pruning is usually applied. Only lateral shoots at the tip which are too steep and too vigorous lateral shoots along the leader are removed in its base. This type of pruning, which promotes fruit bud production and early cropping, reduces vegetative growth of the tree. In the case of good development of the trees after planting , they can be loaded up to 40 fruits in the second growing year, providing a yield of more than 30 tons per hectare. Production in the third leaf can achieve 40-50 t ha-1 and full production, which usually started in the fourth leaf, more than 60 t ha-1can be expected depends on cultivar and growing conditions. Pruning of the mature trees means cutting of the strong watersprouts, the upright shoots and the strong terminal shoots at the top of the tree at their base, remaining only weak fruit-bearing wood. The fruit thinning is regularly applied in modern apple orchards, starting from the second growing year, in order to achieve regular yield and uniform fruit quality.. For this purpose plant growth regulators such as auxins [naphthalene acetic acid (NAA) or naphthalene acetamide (NAAm)] and cytokinin [6 - benzyladenine (BA)] are used. Recently, herbicide metamitron, as a new chemical thinners that at a low dosage reduces photosynthesis and consequently enhances fruit drop are also used. Metamitron exhibited thinning activity when applied to apple fruitlets at the 6 to 15 mm in diameter, or even later, at 20 mm. It can be applied once or twice, depend on the weather conditions in the day of application and three days after
Nonstandard models and analytic equivalence relations
Abstract. We improve a result of Hjorth[93] concerning the nature of thin analytic equivalence relations. The key lemma uses a weakly compact cardinal to construct certain nonstandard models, which Hjorth obtained using #'s. The purpose of this note is to improve the following result of Hjorth [93]. Theorem. (Hjorth) Suppose that for every real x, x # exists. Let E be an analytic equivalence relation, Σ 1 1 in parameter x 0 . Then either there exists a perfect set of pairwise Einequivalent reals or every E-equivalence class has a representative in a set-generic extension of Hjorth's proof makes use of his analysis of nonstandard Ehrenfeucht-Mostowski models built from #'s. Instead, we construct the necessary nonstandard models using infinitary model theory, assuming only the existence of weak compacts. Theorem 1. Suppose that for every real x there is a countable ordinal which is weakly compact in L [x]. Then the conclusion of the Theorem still holds. The main lemma is the following. It is not known if the conclusion of Lemma 2 holds in ZF C alone, for arbitrary x (with ZF replaced by an arbitrary finite subtheory). Proof of Theorem 1 from Lemma 2. Suppose that E is an analytic equivalence relation, Σ 1 1 in the parameter x 0 and choose an x 0 -recursive tree T on ω×ω×ω ω that xEy ←→ T (x, y) has a branch. For each countable ordinal α we define xE α y ←→ rank(T (x, y)) is at least α; then E α is Borel in (x 0 , c) where c is any real coding α and E is the intersection of the E α 's. We may assume that each E α is an equivalence relation. A theorem of HarringtonSilver says that a Π 1 1 -equivalence relation has a perfect set of pairwise inequivalent reals or each equivalence class has a representative constructible from the parameter defining the relation. As E α is Borel in (x 0 , c) where c is a real coding α and as we may assume that E and hence each E α has no perfect set of pairwise inequivalent reals, we know that each E α -equivalence class has a representative in L[x 0 , c] where c is any real coding α. Now let x be arbitrary and by Lemma 2 choose a countable nonstandard ω-model M x of ZF containing (x 0 , x) such that L(M x ) has an isomorphic copy in a set-generic extension N of L[x 0 ]. Let a ∈ ORD(M x ) be nonstandard and let c be a code for a, generic over M x ; then by applying Harrington-Silver in M x [c] we conclude that there is y in L(M x )[x 0 , c] such that yE a x. By choosing c to be generic over N as well we get that y belongs to a set-generic extension of L[x 0 ]. Finally, yEx since if not, yE α x would fail for some α admissible in (y, x) and hence for some (standard) α < a
Međunarodno tržište jabuke - stanje i tendencije
This paper deals with international apple market analysis. Average export
rate was 10,4 million tons (2006-2012) with moderate growth tendency of 2,36%. The
most significant and largest exporter is China, which is the largest producer as well, with
1,5 million tons and 14% share of the world's total export. Then comes Italy, USA,
Chile and France which amount to half of the world's total export, combined. Import is
at 10,1 million tons, at an average rate of 2,5% growth, which represents a trend of slightly
intensive growth comparing to export. The largest importer is Russia, with 1,5 million
tons and 14% share of the world market. Large importers are also Germany, Great
Britain, the Netherlands and France, which absorb one third (1/3) of the world's total
import, combined. For the next period, we can expect further expansion of the international
trade. It will depend mostly on the level of production and financial power of the
consumers - predominantly on economic factors. Trade of organic apples (integral and
organic system of production) shall increase.U radu se analizira međunarodno tržište jabuke. Determinisane su promene
u međunarodnom prometu, apostrofirani su najveći izvoznici i uvoznici. Prosečan izvoz
iznosio je 10,4 miliona tona (2006-2012) sa tendencijom umerenog rasta po stopi od
2,4%. Najznačajniji izvoznik jeste Kina, koja je i najveći svetski proizvođač, sa 1,5 miliona
tona i učešćem od 14% u ukupnom svetskom izvozu. Slede Italija, SAD, Čile i
Francuska koje zajedno daju polovinu svetskog izvoza jabuke. Uvoz iznosi 10,1 miliona
tona i beleži trend nešto intenzivnijeg rasta u odnosu na izvoz, po prosečnoj stopi od
2,5% godišnje. Najveći uvoznik je Rusija sa 1,5 miliona tona i učešćem u svetu od 14%.
Veliki uvoznici su i Nemačka, Velika Britanija, Holandija i Francuska, koje zajedno
apsorbuju trećinu svetskog uvoza. U narednom periodu može se očekivati dalja
ekspanzija međunarodne trgovine. Ona će, pre svega, zavisiti od nivoa proizvodnje i
kupovne moći potrošača, dominantno od ekonomskih faktora. Intenziviraće se trgovina
jabuke proizvedene u integralnom i organskom sistemu proizvodnje
Antioksidativna aktivnost etanolnih ekstrakata Solanum Retroflexum
In this paper the antioxidant activity of ethanolic extracts from Solanum retroflexum
Dun fruits were investigated. The extracts were obtained by classical technique and by
Soxhlet extraction. In order to investigate the possibilities of future utilization of
Solanum retroflexum fruits, the antioxidant activity, by FRAP and DPPH method was
determined. Results showed the extract obtained by 75% ethanol and classic extraction
during 45 minutes at 60°C, had the highest antioxidant activity, both for DPPH and
FRAP method (EC50 was 60,67 μg/mL and 1,55 μmol Fe2+/mg, respectively). There was
good correlation of results for antioxidant activity obtained by both methods and for all
analyzed extracts.Rad se bavi ispitivanjem antioksidativne aktivnosti etanolnih ekstrakata
ploda biljke Solanum retroflexum Dun. Ekstrakti su dobijeni tehnikama klasične i
Soxhlet extrakcije. Potencijalna antioksidativna aktivnost je ispitana FRAP i DPPH
metodama. Rezultati su pokazali da ekstrakt dobijen klasičnom ekstrakcijom sa 75%
rastvorom etanola na 60°C i za vreme 45 minuta, ima najveću antioksidativnu aktivnost
(EC50 = 60,67 μg/ml, 1,55 μmol Fe2+/mg), kao i da postoji dobra korelacija rezultata
dobijenih obema metodama u slučaju svih analiziranih ekstrakata
Caffeine exposure induces browning features in adipose tissue in vitro and in vivo
Brown adipose tissue (BAT) is able to rapidly generate heat and metabolise macronutrients, such as glucose and lipids, through activation of mitochondrial uncoupling protein 1 (UCP1). Diet can modulate UCP1 function but the capacity of individual nutrients to promote the abundance and activity of UCP1 is not well established. Caffeine consumption has been associated with loss of body weight and increased energy expenditure, but whether it can activate UCP1 is unknown. This study examined the effect of caffeine on BAT thermogenesis in vitro and in vivo. Stem cell-derived adipocytes exposed to caffeine (1 mM) showed increased UCP1 protein abundance and cell metabolism with enhanced oxygen consumption and proton leak. These functional responses were associated with browning-like structural changes in mitochondrial and lipid droplet content. Caffeine also increased peroxisome proliferator-activated receptor gamma coactivator 1-alpha expression and mitochondrial biogenesis, together with a number of BAT selective and beige gene markers. In vivo, drinking coffee (but not water) stimulated the temperature of the supraclavicular region, which co-locates to the main region of BAT in adult humans, and is indicative of thermogenesis. Taken together, these results demonstrate that caffeine can promote BAT function at thermoneutrality and may have the potential to be used therapeutically in adult humans
A convergence on Boolean algebras generalizing the convergence on the Aleksandrov cube
We compare the forcing related properties of a complete Boolean algebra B
with the properties of the convergences (the algebraic convergence)
and on B generalizing the convergence on the Cantor and
Aleksandrov cube respectively. In particular we show that is a
topological convergence iff forcing by B does not produce new reals and that
is weakly topological if B satisfies condition
(implied by the -cc). On the other hand, if is a
weakly topological convergence, then B is a -cc algebra or in
some generic extension the distributivity number of the ground model is greater
than or equal to the tower number of the extension. So, the statement "The
convergence on the collapsing algebra B=\ro
((\omega_2)^{<\omega}) is weakly topological" is independent of ZFC
Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides
The mammalian carbohydrate galactose-alpha 1,3-galactose (alpha-Gal) causes a novel form of food allergy, red meat allergy, where patients experience severe allergic reactions several hours after red meat consumption. Here we explored gastric digestion of alpha-Gal glycoproteins using an in vitro model. Bovine thyroglobulin (BTG), a typical alpha-Gal carrying glycoprotein, was digested with pepsin. The resulting peptides were characterised by SDS PAGE, immunoblot and ImmunoCAP using sera from 20 red meat allergic patients. During pepsinolysis of BTG, a wide range of peptide bands was observed of which 14 to 17 kDa peptides remained stable throughout the gastric phase. The presence of the alpha-Gal epitope on the obtained peptides was demonstrated by an anti-alpha-Gal antibody and IgE from red meat allergic patients. The alpha-Gal digests were able to inhibit up to 86% of IgE reactivity to BTG. Importantly, basophil activation test demonstrated that the allergenic activity of BTG was retained after digestion in all four tested patients. Mass spectrometry-based peptidomics revealed that these peptides represent mostly internal and C-terminal parts of the protein, where the most potent IgE-binding alpha-Gal residues were identified at Asn(1756), Asn(1850) and Asn(2231). Thus allergenic a-Gal epitopes are stable to pepsinolysis, reinforcing their role as clinically relevant food allergens
NGS Nominated CELA1, HSPG2, and KCNK5 as Candidate Genes for Predisposition to Balkan Endemic Nephropathy
Balkan endemic nephropathy (BEN) is a familial chronic tubulointerstitial disease with insidious onset and slow progression leading to terminal renal failure. The results of molecular biological investigations propose that BEN is a multifactorial disease with genetic predisposition to environmental risk agents. Exome sequencing of 22 000 genes with Illumina Nextera Exome Enrichment Kit was performed on 22 DNA samples (11 Bulgarian patients and 11 Serbian patients). Software analysis was performed via NextGene, Provean, and PolyPhen. The frequency of all annotated genetic variants with deleterious/damaging effect was compared with those of European populations. Then we focused on nonannotated variants (with no data available about them and not found in healthy Bulgarian controls). There is no statistically significant difference between annotated variants in BEN patients and European populations. From nonannotated variants with more than 40% frequency in both patients' groups, we nominated 3 genes with possible deleterious/damaging variants-CELA1, HSPG2, and KCNK5. Mutant genes (CELA1, HSPG2, and KCNK5) in BEN patients encode proteins involved in basement membrane/extracellular matrix and vascular tone, tightly connected to process of angiogenesis. We suggest that an abnormal process of angiogenesis plays a key role in the molecular pathogenesis of BEN
KIR gene content diversity in four Iranian populations
Killer cell immunoglobulin-like receptors (KIR) regulate natural killer cell response against infection and malignancy. KIR genes are variable in the number and type, thereby discriminating individuals and populations. Herein, we analyzed the KIR gene content diversity in four native populations of Iran. The KIR genomic diversity was comparable between Bakhtiari and Persian and displayed a balance of A and B KIR haplotypes, a trend reported in Caucasian and African populations. The KIR gene content profiles of Arab and Azeri were comparable and displayed a preponderance of B haplotypes, a scenario reported in the natives of America, India, and Australia. A majority of the B haplotype carriers of Azeri and Arab had a centromeric gene-cluster (KIR2DS2-2DL2-2DS3-2DL5). Remarkably, this cluster was totally absent from the American natives but occurred at highest frequencies in the natives of India and Australia in combination with another gene cluster at the telomeric region (KIR3DS1-2DL5-2DS5-2DS1). Therefore, despite having similar frequencies of B haplotypes, the occurrence of B haplotype-specific KIR genes, such as 2DL2, 2DL5, 3DS1, 2DS1, 2DS2, 2DS3, and 2DS5 in Azeri and Arab were substantially different from the natives of America, India, and Australia. In conclusion, each Iranian population exhibits distinct KIR gene content diversity, and the Indo-European KIR genetic signatures of the Iranians concur with geographic proximity, linguistic affinity, and human migrations
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