Partial purification and MALDI-TOF MS analysis of UN1, a tumor antigen membrane glycoprotein.

UN1 is a membrane glycoprotein that is expressed in immature human thymocytes, a subpopulation of peripheral T lymphocytes, the HPB acute lymphoblastic leukemia (ALL) T-cell line and fetal thymus. We previously reported the isolation of a monoclonal antibody (UN1 mAb) recognizing the UN1 protein that was classified as "unclustered" at the 5th and 6th International Workshop and Conference on Human Leukocyte Differentiation Antigens. UN1 was highly expressed in breast cancer tissues and was undetected in non-proliferative lesions and in normal breast tissues, indicating a role for UN1 in the development of a tumorigenic phenotype of breast cancer cells. In this study, we report a partial purification of the UN1 protein from HPB-ALL T cells by anion-exchange chromatography followed by immunoprecipitation with the UN1 mAb and MALDI-TOF MS analysis. This analysis should assist in identifying the amino acid sequence of UN

Conceptual Design of a Variable Geometry, Axial Flow Turbocharger Turbine

The modern automotive industry is under strict regulations to reduce emissions to comply with the Kyoto Protocol, a universally acknowledged treaty aiming at reducing exhaust gas emissions. In order to achieve the required future emission reduction targets, further developments on gasoline engines are required. One of the main methods to achieve this goal is the application of engine downsizing. Turbocharging is a cost-effective method of downsizing an engine whilst reducing exhaust gas emissions, reducing fuel consumption and maintaining prior performance outputs. For these reasons, the turbocharging is becoming the most widely adopted technology in the automotive markets. In 2012, 32% of passenger and commercial vehicles sold had a turbocharger installed, and is predicted to be 40% of 2017 [1]. Even if the engine turbocharging is a widespread technology, there are still drawbacks present in current turbocharging systems. The main problem is overcoming the issue of turbo-lag, which is the poor initial response of the turbocharger to the driver commands due to its inertia. Indeed, the system turbine plus compressor is characterized by an own rotational inertia, therefore, the turbocharger will take a certain time to accelerate and produce the desired boost when a higher amount of exhaust gas is sent to the system. In this work, an innovative solution to the turbo-lag phenomenon will be analyzed: a vaneless stator-axial flow turbine. The proposed turbine configuration would improve the transient response of the system since the axial turbine has intrinsically a lower inertia than the radial turbine as stated by the research works of Ford first [2] and Honeywell after [3]. The whole design process is presented in this paper and particular relevance has been given to the thermo-fluid-dynamic aspect of the machine. Several CFD investigations have been carried out in order to deeply understand the new turbine behavior and, thanks to a 1D model of the target engine, it has been possible to validate the new design simulating the performance of the system engine + turbocharger

2D continuous spectrum of shear Alfven waves in the presence of a magnetic island

The radial structure of the continuous spectrum of shear Alfven modes is calculated in the presence of a magnetic island in tokamak plasmas. Modes with the same helicity of the magnetic island are considered in a slab model approximation. In this framework, with an appropriate rotation of the coordinates the problem reduces to 2 dimensions. Geometrical effects due to the shape of the flux surface's cross section are retained to all orders. On the other hand, we keep only curvature effects responsible of the beta induced gap in the low-frequency part of the continuous spectrum. New continuum accumulation points are found at the O-point of the magnetic island. The beta-induced Alfven Eigenmodes (BAE) continuum accumulation point is found to be positioned at the separatrix flux surface. The most remarkable result is the nonlinear modification of the BAE continuum accumulation point frequency

A multichannel reflectometer for edge density profile measurements at the ICRF antenna in ASDEX upgrade

A multichannel reflectometer will be built for the new three-straps ICRF antenna of ASDEX Upgrade (AUG), to study the density behavior in front of it. Ten different accesses to the plasma are available for the three reflectometer channels that can be interchanged without breaking the machine vacuum. Frequency is scanned from 40 GHz to 68 GHz, in 10 mu s, which corresponds to a cut-off density ranging from 10(18) divided by 10(19)m(-3) in the Right cut-off of the X-mode propagation, for standard toroidal magnetic field values of AUG

Comparison of reflector concepts for a 250 GHz CARM cavity

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Implementation of the new multichannel X-mode edge density profile reflectometer for the ICRF antenna on ASDEX Upgrade

A new multichannel frequency modulated continuous-wave reflectometry diagnostic has been successfully installed and commissioned on ASDEX Upgrade to measure the plasma edge electron density profile evolution in front of the Ion Cyclotron Range of Frequencies (ICRF) antenna. The design of the new three-strap ICRF antenna integrates ten pairs (sending and receiving) of microwave reflectometry antennas. The multichannel reflectometer can use three of these to measure the edge electron density profiles up to 2 x 10(19) m(-3), at different poloidal locations, allowing the direct study of the local plasma layers in front of the ICRF antenna. ICRF power coupling, operational effects, and poloidal variations of the plasma density profile can be consistently studied for the first time. In this work the diagnostic hardware architecture is described and the obtained density profile measurements were used to track outer radial plasma position and plasma shape

The bisphenol a induced oxidative stress in non-alcoholic fatty liver disease male patients: A clinical strategy to antagonize the progression of the disease

Introduction: Bisphenol A (BPA) exposure has been correlated to non-alcoholic fatty liver disease (NAFLD) development and progression. We investigated, in a clinical model, the effects of the administration of 303 mg of silybin phospholipids complex, 10 μg of vitamin D, and 15 mg of vitamin E (RealSIL, 100D, IBI-Lorenzini, Aprilia, Italy) in male NAFLD patients exposed to BPA on metabolic, hormonal, and oxidative stress-related parameters. Methods: We enrolled 32 male patients with histologic diagnosis of NAFLD and treated them with Realsil 100D twice a day for six months. We performed at baseline clinical, biochemical, and food consumption assessments as well as the evaluation of physical exercise, thiobarbituric acid reactive substances (TBARS), plasmatic and urinary BPA and estrogen levels. The results obtained were compared with those of healthy control subjects and, in the NAFLD group, between baseline and the end of treatment. Results: A direct proportionality between TBARS levels and BPA exposure was shown (p < 0.0001). The therapy determined a reduction of TBARS levels (p = 0.011), an improvement of alanine aminotransferase, aspartate aminotransferase, insulinemia, homeostatic model assessment insulin resistance, C reactive protein, tumor necrosis factor alpha (p < 0.05), an increase of conjugated BPA urine amount, and a reduction of its free form (p < 0.0001; p = 0.0002). Moreover, the therapy caused an increase of plasmatic levels of the native form of estrogens (p = 0.03). Conclusions: We highlighted the potential role of BPA in estrogen oxidation and oxidative stress in NAFLD patients. The use of Realsil 100D could contribute to fast BPA detoxification and to improve cellular antioxidant power, defending the integrity of biological estrogen-dependent pathways

The tunable resonant IC antenna concept and its design for DTT experiment

The intrinsic poor loading of Ion Cyclotron (IC) plasma-facing antennas makes the use of Tuning and Matching Systems (TMSs) a necessity. The antenna plus TMS is a resonant system; in the TMS and access lines high voltages (tens of kV) must be accounted for in the unavoidable unmatched part of the feeding lines. In this work, we propose and test an innovative type of IC launcher; it is based on achieving resonance of the self-standing antenna, i.e. without the TMS. A mechanical full-metal tuning mechanism is described and demonstrated to allow wide-band operation. A systematic analysis of possible antenna topologies has led to identifying a structure that can allow good impedance matching along with compliance with maximum electric field constraints. Most of the design is carried out using a simplified plasma and a commercial analysis tool and then validated with a realistic plasma using TOPICA code