Akzeptanz von Armprothesen im Kindes -und Jugendalter

Aufgabe dieser Arbeit ist es, objektivierbare Kriterien über die Compliance des kindlichen Prothesentrageverhaltens bei fehlenden Gliedmaßen zu finden. Dazu wurde eine retrospektive Analyse von Daten aus Krankenblättern und Fragebögen armprothetisch versorgter Patienten vorgenommen, die im Zeitraum von 1984 bis 2004 in der Klinik für Technische Orthopädie und Rehabilitation der WWU Münster behandelt worden sind. Die Daten enthalten Angaben über das Geschlecht, das Alter, das Alter bei Erstvorstellung und Erstversorgung, das Prothesentrageverhalten, die Tragedauer, Ablehnungsgründe, die Stigmatisierung der Eltern und der Kinder und das Umfeld der Kinder. Die Ergebnisse zeigen, dass das Geschlecht der Patienten, die jeweilige Diagnose, das Alter bei der Erstversorgung, die Verordnung, die Stigmatisierung der Eltern und der Kinder Einfluss auf deren Compliance und die daraus resultierende Akzeptanz der Prothese haben. Die Ergebnisse decken sich mit der Literatur der letzten Jahre

In situ conservation—harnessing natural and human-derived evolutionary forces to ensure future crop adaptation

Ensuring the availability of the broadest possible germplasm base for agriculture in the face of increasingly uncertain and variable patterns of biotic and abiotic change is fundamental for the world's future food supply. While ex situ conservation plays a major role in the conservation and availability of crop germplasm, it may be insufficient to ensure this. In situ conservation aims to maintain target species and the collective genotypes they represent under evolution. A major rationale for this view is based on the likelihood that continued exposure to changing selective forces will generate and favor new genetic variation and an increased likelihood that rare alleles that may be of value to future agriculture are maintained. However, the evidence that underpins this key rationale remains fragmented and has not been examined systematically, thereby decreasing the perceived value and support for in situ conservation for agriculture and food systems and limiting the conservation options available. This study reviews evidence regarding the likelihood and rate of evolutionary change in both biotic and abiotic traits for crops and their wild relatives, placing these processes in a realistic context in which smallholder farming operates and crop wild relatives continue to exist. It identifies areas of research that would contribute to a deeper understanding of these processes as the basis for making them more useful for future crop adaptation

Monkeypox virus – Would it arrive in Latin America?

May 2022 mark the beginning of a new outbreak of some degree of concern, globally, as it is spreading relatively quickly in multiple countries, between persons even without travel-related history [1]. Monkeypox, a DNA virus member of the Orthopoxvirus genus (family Poxviridae) (Figure 1), is now the cause of clinical disease in almost 200 hundred suspected cases in more than a dozen of countries (Figure 2) outside Africa, where this zoonosis is endemic, especially in the Democratic Republic of the Congo, in the Republic of the Congo and Nigeria. As the number of cases increases daily, the question in Latin America is quite apparent. Would Monkeypox arrive in Latin America? Yes. When writing this Editorial, a suspected case is already investigated in Argentina. A traveller from Spain, with clinical findings compatible with Monkeypox infection. Then, as has occurred with the COVID-19 pandemic [2], we should expect to have more suspected Monkeypox cases in Argentina, as well as, in other countries, especially in those with a large volume of international flights between them and North America and Europe, as is the case of Brazil, Mexico, Colombia, Chile, Peru, among others in the region

Psychological morbidity, social intimacy, physical symptomatology, and lifestyle in adult children of divorced parents

This study assessed the relationships between psychological morbidity, social intimacy, perception of the parental relationship, lifestyle and physical symptomatology as well as the contributors of physical symptomatology, in adult children of divorced parents. Participants answered the Physical Symptom Questionnaire, the Anxiety, Depression and Stress Scale, the Lifestyle Questionnaire, the Social Intimacy Scale and The Perception of the Parental Relationship Scale. Results revealed that older participants showed lower levels of anxiety, depression and stress and that male participants perceived the parental relationship as better when compared to females. There was a negative association between depression and the duration of parental divorce. Adult children with greater physical symptoms and more anxious showed less social intimacy. The latter was associated with a less healthy lifestyle. As expected less physical symptomatology was associated with lower levels of depression, anxiety, and stress. Anxiety was the only variable that contributed to physical symptomatology. Intervention should be gender sensitive and focus early on on the identification of psychological morbidity, social intimacy, healthy behaviors, and physical symptomatology, in adult children whose parents are in the process of divorcing.(undefined

Cumulative regulatory potential of clustered methyl-arginine protein modifications

Systematic analysis of human arginine methylation events bifurcates its signaling mechanism, functioning either in isolation akin to canonical PTM regulation or clustered within disordered protein sequence. Hundreds of proteins contain methyl-arginine clusters and are more prone to mutation and more tightly expression-regulated than dispersed methylation targets. Arginine clusters in the highly methylated RNA binding protein SYNCRIP were experimentally shown to function in concert providing a tunable protein interaction interface. Quantitative immuno-precipitation assays defined two distinct cumulative regulatory mechanisms operating across 18 proximal arginine-glycine motifs in SYNCRIP. Functional binding to the methyl-transferase PRMT1 was promoted by continual arginine stretches while interaction with the methyl-binding protein SMN1 was arginine content dependent irrespective of linear position within the unstructured region. This study highlights how highly repetitive di-amino acid motifs in otherwise low structural complexity regions can provide regulatory potential, and with SYNCRIP as an extreme example how PTMs leverage these disordered sequences to drive cellular functions

Influence of Pacing Mode and Rate on Peripheral Levels of Atrial Natriuretic Peptide (ANP)

The effect of acute modifications of pacing mode and rate on plasma ANP levels was evaluated. ANP was determined in ten resting patients with ODD pacemokers due to binodal disease or intermittent second- and third-degree AV block. At 82/minute pacing rate the ANP plasma levels (normal range 2 to 30 fmol/mL) corresponded to those under AAI (4.05 ± 2.10 fmol/mL) and DDD (4.18 ± 2.02 fmol/mL) pacing, but increased significantly (P < 0.05) during VVI pacing (6.96 ± 3.70 fmol/mL). Acceleration of DDD stimulation frequency from 82 to 113/minutes led to significant increases of ANP levels by the factor of three in all chosen AV delays. The lowest ANP plasma levels were measured of 175 msec AV delay under 82/minute pacing rate in DDD mode. Under 113/minutes the differences of ANP concentration after variations of AV delays were less pronounced. The influences of altered atrial pressure and tension on ANP release are discussed to account for changes in ANP plasma levels following different modes and rates of pacemaker stimulation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75366/1/j.1540-8159.1989.tb01862.x.pd

Flexible and scalable diagnostic filtering of genomic variants using G2P with Ensembl VEP.

We aimed to develop an efficient, flexible and scalable approach to diagnostic genome-wide sequence analysis of genetically heterogeneous clinical presentations. Here we present G2P ( www.ebi.ac.uk/gene2phenotype ) as an online system to establish, curate and distribute datasets for diagnostic variant filtering via association of allelic requirement and mutational consequence at a defined locus with phenotypic terms, confidence level and evidence links. An extension to Ensembl Variant Effect Predictor (VEP), VEP-G2P was used to filter both disease-associated and control whole exome sequence (WES) with Developmental Disorders G2P (G2PDD; 2044 entries). VEP-G2PDD shows a sensitivity/precision of 97.3%/33% for de novo and 81.6%/22.7% for inherited pathogenic genotypes respectively. Many of the missing genotypes are likely false-positive pathogenic assignments. The expected number and discriminative features of background genotypes are defined using control WES. Using only human genetic data VEP-G2P performs well compared to other freely-available diagnostic systems and future phenotypic matching capabilities should further enhance performance