Interrelations between changes in parameters of HRV, EEG and humoral immunity at patients with chronic pyelonephritis and cholecystitis

Kul’chyns’kyi Andriy B, Zukow Walery, Korolyshyn Tetyana A, Popovych Igor L. Interrelations between changes in parameters of HRV, EEG and humoral immunity at patients with chronic pyelonephritis and cholecystitis. Journal of Education, Health and Sport. 2017;7(9):439-459. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1001856 http://ojs.ukw.edu.pl/index.php/johs/article/view/4933 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2017; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.09.2017. Revised 10.09.2017. Accepted: 10.09.2017. INTERRELATIONS BETWEEN CHANGES IN PARAMETERS OF HRV, EEG AND HUMORAL IMMUNITY AT PATIENTS WITH CHRONIC PYELONEPHRITIS AND CHOLECYSTITIS Andriy B Kul’chyns’kyi1,2, Walery Zukow3, Tetyana A Korolyshyn4, Igor L Popovych4 1Communal Hospital, Truskavets’, Ukraine [email protected] 2Ukrainian Scientific Research Institute of Transport Medicine, Odesa 3Nicolaus Copernicus University, Torun’, Poland [email protected] 4Department of Immunophysiology, OO Bohomolets’ Institute of Physiology, Kyiv, Ukraine [email protected] Abstract Background. The immunological homunculus conception considers somatotopic organization to СNS regulation of immune system. Previously, we have shown within the immunological homunculus conception that take place causal relationships parameters of EEG and HRV, on the one hand, and the parameters of cellular and humoral immunity as well as phagocytose, on the other hand. The purpose of this study is to find out the relationships between induced by balneotherapy changes in parameters of EEG and HRV, on the one hand, and the parameters of immunity, on the other hand. Methods. In basal conditions in 33 men and 10 women with chronic pyelonephritis and cholecystitis in remission, we recorded twice, before and after balneotherapy at the spa Truskavets’, EEG (“NeuroCom Standard”) and HRV (“Cardiolab+VSR”). In blood estimated routine parameters of Humoral Immunity on a set of I and II levels recommended by the WHO. Results. We detected that changes in relative level CD22+ B-Lymphocytes upregulated by parasympathetic outflows and β-rhythm generating structures that are projected onto the loci O2, Fp1, F3 and F4, while downregulated by sympathetic outflows and θ-rhythm generating structures that are projected onto the locus T5. Serum level of IgA upregulated by parasympathetic outflows and α-rhythm generating structures that are projected onto the loci T3 and C3 as well as δ-rhythm generating structures onto F7 and T5, while downregulated by sympathetic outflows and θ-rhythm generating structures that are projected onto the loci T5, F7, Fp2, F3, F8 and F4. Serum level of CIC upregulated by parasympathetic outflows and α-rhythm generating structures that are projected onto the loci T4, Fp2, T3, F8 and C3 as well as θ-rhythm generating structures onto the loci T3 and T6, while downregulated by sympathetic outflows and β-rhythm generating structures that are projected onto the loci Fp1, F3 and P4 as well as δ-rhythm generating structures onto T4. Unlike the previous parameters serum level of IgM downregulated by parasympathetic outflows and α-rhythm generating structures that are projected onto the loci T3 and T6 as well as β-rhythm generating structures onto the locus T5, while upregulated by sympathetic outflows and θ-rhythm generating structures that are projected onto the loci F3, Fp2, T5, F4, O1 and F8. Serum level of IgG downregulated by parasympathetic outflows, while upregulated by sympathetic outflows and δ-rhythm generating structures that are projected onto the loci O2 and F8 as well as α-rhythm generating structures onto C3. Conclusion. The results are supplemented and refined Tracey’s immunological homunculus conception. Keywords: HRV, EEG, B-Lymphocytes, Immunoglobulines M,G,A, Circulating immune complexes, correlations

Interrelations between changes in parameters of HRV, EEG and cellular immunity at patients with chronic pyelonephrite and cholecystite

Background. Previously we have shown within the immunological homunculus conception that take place causal relationships between induced by balneotherapy changes in parameters of EEG and HRV, on the one hand, and the parameters of humoral immunity, on the other hand. The purpose of this study is to find out the relationships between induced by balneotherapy changes in parameters of EEG and HRV, on the one hand, and the parameters of cellular immunity, on the other hand. Methods. In basal conditions in 33 men and 10 women with chronic pyelonephritis and cholecystitis in remission, we recorded twice, before and after balneotherapy at the spa Truskavets’, EEG (“NeuroCom Standard”) and HRV (“Cardiolab+VSR”). In blood estimated routine parameters of cellular immunity. Results. We detected that changes in relative level CD4+ T helper lymphocytes upregulated by parasympathetic outflows and β-rhythm generating structures that are projected onto the loci T3, T4 and P4, while downregulated by sympathetic outflows and β-rhythm generating structures that are projected onto the locus F7 as well as α-rhythm generating structures onto T6 and P4 loci. Level of CD8+ T cytolytic lymphocytes vice versa downregulated by parasympathetic outflows as well as δ-rhythm generating structures onto locus F7 and θ-rhythm generating structures onto the locus T3, while upregulated by θ-rhythm generating structures onto the locus T4. Level of CD16+ natural killers upregulated by sympathetic outflows and β-rhythm generating structures that are projected onto the loci T4, T5 and C3 as well as α-rhythm generating structures onto the locus T4, while downregulated by parasympathetic outflows and β-rhythm generating structures that are projected onto the loci Fp1 and F4 as well as α-rhythm generating structures onto the loci P4, O1 and O2, θ-rhythm generating structures onto the loci F7, T5, T6 and O2 as well as δ-rhythm generating structures that are projected onto the loci F7, T5 and T6. Level of 0-lymphocytes upregulated by sympathetic outflows and δ-rhythm generating structures that are projected onto the loci F7 and T5 as well as α-rhythm generating structures onto the loci P4 and O2, while downregulated by β-rhythm generating structures that are projected onto the loci T5 and O1. Conclusion. The results are supplemented and refined Tracey’s immunological homunculus conception

Interrelations between changes in parameters of HRV, EEG and phagocytosis at patients with chronic pyelonephritis and cholecystitis

Background. Previously we have shown within the immunological homunculus conception that take place causal relationships between induced by balneotherapy changes in parameters of EEG and HRV, on the one hand, and the parameters of humoral and cellular immunity, on the other hand. The purpose of this study is to find out the relationships between induced by balneotherapy changes in parameters of EEG and HRV, on the one hand, and the parameters of phagocytosis , on the other hand. Material and methods. In basal conditions in 33 men and 10 women with chronic pyelonephritis and cholecystitis in remission, we recorded twice, before and after balneotherapy at the spa Truskavets’, EEG (“NeuroCom Standard”) and HRV (“Cardiolab+VSR”). In blood estimated parameters of phagocytic function of neutrophils against Staphylococcus aureus and Escherichia coli. Results. Judging by the coefficients of canonical correlation, the maximal subordinate nervous regulation is the completeness of phagocytosis of E. coli (R=0,944), whereas the influence of the nervous system on the completeness of phagocytosis Staph. aur. is minimal (R=0,607). With regard to the intensity of phagocytosis, the differences in the strength of nerve influence are expressed less (0,893 vs 0,744), and in relation to the activity of phagocytosis as well as the bactericidal ability of neutrophils, there are no differences between R (0,858 vs 0,857 and 0,831 vs 0,834, respectively). However, significant differences were found between profiles of correlation coefficients between EEG and HRV parameters, on the one hand, and phagocytosis parameters, on the other hand, for representatives of Gram-negative and Gram-positive microbes. Conclusion. In the nervous regulation of phagocytosis by neutrophils of the Gram-negative and Gram-positive microbes are involved the structures that are localized in different parts of the CNS

Influence of the course of electrostimulation by the device "VEB-1" on parameters of electroencephalogramm at practically healthy males

Background . We created and patented device for electrostimulation "VEB-1". It is intended for activation of functional systems of organism by wave influence on nerve plexus by frequency beats method. This article launches a series of articles on the influence of this device on the parameters of the neuroendocrine-immune complex and the metabolism of various categories of people. Materials and Methods. The object of observation were 14 males aged 24-59 years without clinical diagnose but with dysfunction of neuro -endocrine- immune complex and metabolism. In the basal conditions we recorded electroencephalogram (EEG) a hardware-software complex “NeuroCom Standard” (KhAI Medica, Kharkiv, Ukraine ). Then the volunteers were subjected to an electrostimulation session lasted for 21 minutes in four days. One day after the last session, the EEG was re-registered. Results. 20 parameters of EEG were identified, in which the volunteers' neurodynamics before and after the course of electrostimulation differed considerably (Squared Mahalanobis Distance make up 191; F=16,7; p<10^-3 ). The neurotropic stimulation effect has a modulating character, namely: the initially decreased spectral power density (SPD) of the alpha-rhythm in F3, F4, T4, T5 loci as well as of theta-rhythm in P3 locus increases; decreased SPD of beta-rhythm in the F3, C3, C4, P3, P4 and O2 loci as well as Amplitude of beta-rhythm becomes even smaller; the initially increased SPD of delta-rhythm in the loci Fp1, F8 and P4 rises further. Conclusion. A four-day electrostimulation course causes on males with dysfunction of the neuro-endocrine-immune complex and metabolism a notable neuro-modulating effect evaluated by changes in basal EEG

Gas Discharge Visualization (Electrophotonic Imaging, Kirlianography). Theoretical and Applied Aspects, 189 s.

The monograph highlights the results of priority clinical-physiological studies of the relationships between gas discharge visualization (electrophotonic imaging, kirlianography) parameters, on the one hand, and electroencephalograms, heart rate variability, immunograms, phagocytosis, the content of the main adaptation hormones (cortisol, aldosterone, testosterone, triiodothyronine, calcitonin) in the blood as well as acupuncture points - on the other hand. It is shown that the GDV/EPI method reliably reflects the state of the body's neuro-endocrine-immune complex as well as others parameters and has the right to take its place in the arsenal of physiological/biophysical methods. For biophysicists, physiologists, psychophysiologists, endocrinologists, immunologists, medical rehabilitation specialists. INTRODUCTION Advances in biophysics, biology, functional genomics, neuroscience, psychology, psychoneuroimmunology, and other fields suggest the existence of a subtle system of “biofield” interactions that organize biological processes from the subatomic, atomic, molecular, cellular, and organismic to the interpersonal and cosmic levels. Biofield interactions may bring about regulation of biochemical, cellular, and neurological processes through means related to electromagnetism, quantum fields, and perhaps other means of modulating biological activity and information flow. The biofield paradigm, in contrast to a reductionist, chemistry-centered viewpoint, emphasizes the informational content of biological processes; biofield interactions are thought to operate in part via low-energy or “subtle” processes such as weak, nonthermal electromagnetic fields (EMFs) or processes potentially related to consciousness and nonlocality. Biofield interactions may also operate through or be reflected in more well-understood informational processes found in EEG and ECG data. Recent advances have led to the development of a wide variety of therapeutic and diagnostic biofield devices, defined as physical instruments best understood from the viewpoint of a biofield paradigm [Muehsam D et al, 2015]. Biofield devices comprise physical instruments that may be most clearly understood from the viewpoint of a biofield paradigm, and a large and diverse number of devices have been developed to measure or manipulate biofield interactions. These include both diagnostic devices (to measure biofield properties) and therapeutic devices (to manipulate biofield interactions). The study of biofield devices is at a nascent stage of development, and much further research is needed to determine clinical efficacy and elucidate the underlying mechanisms of action for many of the devices mentioned here. The biofield devices operate through a variety of modalities rather than a single mechanism. Some biofield devices function through well-understood mechanisms and are already widely used in clinical settings: for example, electroencephalography (EEG)- and electrocardiography (ECG)-based heart rate variability (HRV). Other devices appear to operate through mechanisms that are novel or incompletely understood. However, all of these devices share a common property: rather than functioning primarily in a reductionist, chemistry-centered manner, biofield devices function via the informational content of biological processes and can interact via low-energy or “subtle” processes, including those potentially related to consciousness and nonlocality [Muehsam D et al, 2015]. Here Muehsam D et al [2015] provide a brief overview of the broad categories of biofield devices, with the goal being to stimulate further discussion and research. Authors describe those devices for which thay deemed that sufficient evidence exists to warrant mention. They chose to focus upon devices for which peer-reviewed scientific reports suggesting efficacy are available rather than conference proceedings or manufacturers' white papers. However, in the few cases that specific devices with sufficient promise and relevance lacked a peer-reviewed basis, authors have presented whatever evidence was available. Here, devices are organized according to mode of operation and these modalities include electromagnetic field (EMF)-light, EMF-heat, EMF-nonthermal, electrical current, vibration and sound, physical and mechanical, intentionality and nonlocality, gas and plasma, and other (mode of operation not well understood). Muehsam D et al [2015] deemed that gas discharge visualization (GDV) is an important example of the use of plasma in biofield science. Back in 1880 Nikola Tesla demonstrated that when placing the man in the high-frequency field around the body there is a bright glow [cit. by Korotkov KG, 2001]. In 1892 Nardkevych-Yodko YO recorded glow human hands on photographic plate [cit. by Ciesielska I, 2009]. However, a well-known method of "high-frequency photography" was due to spouses Kirlian SD&VH who in 1939 independently discovered this phenomenon [Kirlian SD & Kirlian VKh, 1961], later called "Kirlian’s effect". This technique has been called corona discharge photography [Boyers DG & Tiller WA, 1973], electrophotography [Earle L, 1975], electrography [Konikiewicz LW, 1979], GDV [Bankovskii NG et al, 1986]. In 1996 Korotkov KG created a new scientific approach, based on the digital videotechnics, modern electronics and computer processing quantitative data, called as method gas discharge visualization (GDV bioelectrography). Parallel uses the terms Kirlianography and Electrophotonic imaging (EPI) [Korotkov KG, 2001; 2007; 2014; Korotkov KG et al, 2002; Wisneski LA & Anderson L, 2009; Jakovleva E & Korotkov K, 2013]. Method of GDV, essence of which consists in registration of photoelectronic emission of skin, induced by high-frequency electromagnetic impulses, allows to estimate integrated psycho-somatic state of organism. The first base parameter of GDV is Area of Gas Discharge Image (GDI) in Right, Frontal and Left projections registered both with and without polyethylene filter. The second base parameter is a coefficient of form/shape (ratio of square of length of external contour of GDI toward his area), which characterizes the measure of serration/fractality of external contour. The third base parameter of GDI is Entropy, id est measure of chaos. It is considered that GDI, taken off without filter, characterizes the functional changes of organism, and with a filter characterizes organic changes. Program estimates also Energy and Asymmetry of virtual Chakras [Korotkov KG, 2001; 2007; 2014]. Nearly 1000 papers have been published (mostly in Russian) on GDV research and a few hundred more in the West. These intriguing data suggest that informatics based upon biofield measurement devices such as the GDV may be useful for gaining deeper understanding of disease states and guiding practitioners and their patients towards states of greater wellness [Muehsam D et al, 2015]. Without regard to the wideuse enough of method in medicine, psychology, valeology and others like that, he yields to the just criticizing for an insufficient physiology ground. There fore we put before itself sweep to analyse relationships between the parameters of GDV - from one side, and by the row of neurodynamics, endocrine, immune. psychophysiological, and other parameters - on the other hand

Similarity Of Effects On EEG Parameters Of Aramaic, Greek Catholic And Krishnaic Prayers

The neurotropic effects of Prayer are manifested in two inhibitory and three activating patterns. The first inhibitory pattern reflects the decrease in elevated and upper limit levels of SPD of θ-and δ-rhythm in frontal loci and the second - decrease in normal SPD levels of β-and θ-rhythm in the frontal, central, temporal and parietal loci. The first activating pattern reflects a small increase in normal levels of β-rhythm index and asymmetry and SPD entropy in locus C3, as well as a further increase in elevated δ-rhythm SPD levels in loci P3 and T3. The second pattern reflects the slight increase in normal SPD levels of θ-rhythm in loci T3, T5, T6, O2 and α-rhythm in locus T5, as well as their indices and entropy SPD in locus O2. The third pattern reflects a slight increase in amplitude and SPD of α-rhythm in central, frontal, temporal and occipital loci

Neuro-immune relationships at patients with chronic pyelonephrite and cholecystite. Communication 2. Correlations between parameters EEG, HRV and Phagocytosis

Kul’chyns’kyi Andriy B, Kovbasnyuk Marta M, Korolyshyn Tetyana A, Kyjenko Valeriy M, Zukow Walery, Popovych Igor L. Neuro-immune relationships at patients with chronic pyelonephrite and cholecystite. Communication 2. Correlations between parameters EEG, HRV and Phagocytosis. Journal of Education, Health and Sport. 2016;6(10):377-401. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.163221 http://ojs.ukw.edu.pl/index.php/johs/article/view/3957         The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.10.2016. Revised 02.10.2016. Accepted: 21.10.2016.       Neuro-immune relationships at patients with chronic pyelonephrite and cholecystite. Communication 2. Correlations between parameters EEG, HRV and Phagocytosis   Andriy B Kul’chyns’kyi1, Marta M Kovbasnyuk2, Tetyana A Korolyshyn2, Valeriy M Kyjenko2, Walery Zukow3, Igor L Popovych2   1Communal Hospital, Truskavets’, Ukraine  [email protected] 2Laboratory of Experimental Balneology, OO Bogomoletz Institute of Physiology NAS, Kyiv, Ukraine  [email protected]   [email protected] 3Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland  [email protected]   Abstracts Background. Previously, we have shown that blood level of Leukocytes and parameters of Leukocytogram significantly modulated by electrical activity some structures of central and autonomic nervous systems. In this study, we set a goal to analyse the relationships between parameters of EEG and HRV, on the one hand, and the parameters of phagocytosis, on the other hand. Methods. In basal conditions in 23 men, patients with chronic pyelonephrite and cholecystite in remission, recorded EEG (“NeuroCom Standard”, KhAI Medica, Ukraine) and HRV (“Cardiolab+VSR”, KhAI Medica, Ukraine). In portion of venous blood estimated parameters of phagocytic function of neutrophils. Results. Revealed that canonical correlation between constellation EEG and HRV parameters form with Phagocytic Index 0,94 and 0,93 for E. coli and Staph. aureus respectively. However neural regulation Killing Index as well as Microbial Count significantly distinguished for Gram-negative and Gram-positive Bacteria: R form 0,99 and 0,84 versus 0,66 and 0,69 respectively. Integrated parameter Bactericidity Capacity of blood Neutrophills correlated with EEG and HRV parameters equally: R makes 0,89 and 0,93 respectively. Conclusion. Neutrophils phagocytic function subordinate modulation central and autonomic nervous systems. Keywords: HRV, EEG, Phagocytosis, Escherichia coli, Staph. aureus, correlations

Relationships between caused by Kozyavkin© method changes in parameters of manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic form of cerebral palsy

Background. Earlier we reported that in children with spastic forms of cerebral palsy (SFCP) after two-week course of rehabilitation by Kozyavkin© method reducing neural component of muscle tone (NCMT) stated in 79,3% cases while in 13,8% cases changes were not detected and in 2 children it increased. We hypothesized that such a variety of changes in NCMT is due to ambiguous changes in the background activity of the nerve centers. Aim: analysis of relationships between changes (Ch) in NCMT as well as manual functional tests, on the one hand, and parameters of EEG, HRV as well as Gas Discharge Visualization (GDV), on the other hand. Material and research methods. The object of observations were 14 children (6 girls and 8 boys) aged 8÷15 years with SFCP. State motor development at GMFCS was on II÷IV level. Functional status of the hand with MACS was at II÷III level. The estimation of hand function carried out by Dynamometry (D), Box and Block Test (B&B) and Nine Hole Peg Test (NHP). We registered also NCMT by device “NeuroFlexor” (Aggero MedTech AB, Sweden), HRV and EEG simultaneosly by hardware-software complex “Cardiolab+VSR” and “NeuroCom Standard” respectively (KhAI Medica, Kharkiv, Ukraine) as well as GDV by “GDV Chamber” (“Biotechprogress”, St-Pb, RF). Results. After two-week course of rehabilitation at 9 children NCMT reduced from 19,8±3,4 to 12,3±2,8 Newtons (Ch: -7,5±2,0 N), at 3 children NCMT taked 8,2±3,3 before and 7,9±3,5 after rehabilitation (Ch: -0,3±0,3 N) while at one girl NCMT increased from 15,1 to 17,9 N and at one boy from 6,1 to 19,4 N. Manual functional tests also changed ambiguously. The Ch in NCMT are correlated with Ch in parameters HRV&EEG (R2=0,786). The Ch in functional tests of Left hand are correlated with Ch in parameters HRV&EEG to the same extent: the level of R2 is for D 0,799, for NHP 0,773 and for B&B 0,708. Instead, for the Right hand, the correlation is stronger: R2 is 0,973, 0,792 and 0,978 respectively. As regards GDV parameters, connections are weaker, but they are also stronger for the Right hand: R2 is 0,706 vs 0,462 for B&B and 0,679 vs 0,405 for NHP but not for D (0,719 and 0,709). The Ch in NCMT are correlated with Ch in parameters GDV also weaker (R2=0,556). In its turn, changes in GDV parameters are very closely related to changes in parameters of both HRV (R2=0,999) and EEG (R2=0,998). Conclusion. In children with spastic forms of cerebral palsy caused by Kozyavkin© method changes in manual functional tests and neural component of muscle tone are determined by changes in parameters of EEG and HRV as well as GDV

Comparative investigation effects on nervous and immune systems of bioactive water Naftussya Spa Truskavets’ and stable water solution of Boryslav’s Ozokerite

Popovych Igor L, Gumega Myroslav D, Verba Igor E, Popovych Andriǐ I, Korolyshyn Tetyana A, Tkachuk Svitlana P, Ostapenko Volodymyr M, Zukow Walery. Comparative investigation effects on nervous and immune systems of bioactive water Naftussya Spa Truskavets’ and stable water solution of Boryslav’s Ozokerite. Journal of Education, Health and Sport. 2016;6(4):364-374. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.50476 http://ojs.ukw.edu.pl/index.php/johs/article/view/3486   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 20.04.2016. Revised 25.04.2016. Accepted: 25.04.2016.   COMPARATIVE INVESTIGATION EFFECTS ON NERVOUS AND IMMUNE SYSTEMS OF BIOACTIVE WATER NAFTUSSYA SPA TRUSKAVETS’ AND STABLE WATER SOLUTION OF BORYSLAV’S OZOKERITE   Igor L Popovych1, Myroslav D Gumega2, Igor E Verba3, Andriǐ I Popovych1, Tetyana A Korolyshyn1, Svitlana P Tkachuk2, Volodymyr M Ostapenko2, Walery Zukow4   1Laboratory of Experimental Balneology, OO Bohomolets’ Institute of Physiology NASU, Kyїv, Ukraine  [email protected] 2Sanatorium “Kryshtalevyǐ Palats” SAD, Truskavets’, Ukraine  [email protected] 3Scientific Company “Verba”, Kyїv, Ukraine  [email protected] 4Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland  [email protected]       Abstract   Background. It is known that biological activity of curative water Naftussya spa Truskavets’ (Ukraine) caused by its organic substances, genetically connected with Oil and Ozokerite from Boryslav’s layer, located near from Truskavets’. The low stability of water Naftusya defined our trying to reproduce its natural receiving in laboratory conditions. Aim: compare effects on nervous and immune systems of natural bioactive water Naftussya (BAWN) spa Truskavets’ and stable water solution of the Ozokerite, extracted from the Boryslav’s field. Material and research methods. The clinical approbation involved 27 volunteers with disfunction of immune and autonomous nervous system. Immune status evaluated on a set of I and II levels recommended by the WHO. About phagocytic function of neutrophils judged by activity, intensity and completeness phagocytose of Staphylococcus aureus and Escherichia coli. About state of autonomous and central nervous system judged by parameters of Heart Rate Variability (“CardioLab+HRV”) and Electroencephalogram (“NeuroCom Standard”). After testing 20 volunteers drinked BAWN while 7 others volunteers simultaneously drinked Ozokerite extract during 7 days. Daily entering of organic substances (as C organic) for both groups makes 0,3 mg/kg. Results. By method of discriminant analysis we detected 7 immune and 3 neural variables changes in which are almost identical in both groups. In particular, increases reduced levels Bacterocidity against Staph. aur., Killing Index against E. coli, Circulating Immune Complexes and Asymmetry of δ-Rhythm as well as normal levels Entropy of Immunocytogram and Immunity Integral Index while decreases raised levels Sympathetic tone, Centralization Index HRV and IgM of serum. Among variables currently not in the model it is necessary to note increasing reduced levels Bacterocidity against E. coli and Vagal tone as well as normal levels Microbial Count for Staph. aur., “active” T-Lymphocytes and Deviation α-Rhythm Frequency while decreasing raised levels Bayevskiy’s Stress Index and Activity Regulatory Systems Index HRV. Conclusion. Course drinking of stable water solution of the Ozokerite, extracted from the Boryslav’s field, imitates favourable effects of bioactive water Naftussya spa Truskavets’ on parameters of Immune and Autonomous Nervous systems at volunteers with their disfunction.   Keywords: bioactive water Naftussya, Ozokerite, Immune and Autonomous Nervous systems

Features of immunity by various constellations of principal adaptation hormones and autonomous regulation in practically healthy people

Gozhenko Anatoliy I, Hrytsak Yaroslav L, Barylyak Liliya G, Kovbasnyuk Marta M, Tkachuk Svitlana P, Korolyshyn Tetyana A, Matiyishyn Galyna Yo, Zukow Walery, Popovych Igor L. Features of immunity by various constellations of principal adaptation hormones and autonomous regulation in practically healthy people. Journal of Education, Health and Sport. 2016;6(10):215-235. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.160979 http://ojs.ukw.edu.pl/index.php/johs/article/view/3936         The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.10.2016. Revised 02.10.2016. Accepted: 15.10.2016.       FEATURES OF IMMUNITY BY VARIOUS CONSTELLATIONS OF PRINCIPAL ADAPTATION HORMONES AND AUTONOMOUS REGULATION IN PRACTICALLY HEALTHY PEOPLE   Anatoliy I Gozhenko1, Yaroslav L Hrytsak2, Liliya G Barylyak2,3,4, Marta M Kovbasnyuk4, Svitlana P Tkachuk3, Tetyana A Korolyshyn4, Galyna Yo Matiyishyn3, Walery Zukow5, Igor L Popovych4   1Ukrainian Scientific Research Institute of Transport Medicine Ministry of Health, Odesa   [email protected] 2Hotel Spa Complex “Karpaty”, Truskavets’, Ukraine  [email protected] 3JSC “Truskavets’kurort”, Truskavets’, Ukraine   [email protected] 4Laboratory of Experimental Balneology, OO Bogomoletz Institute of Physiology NAS, Kyiv, Ukraine  [email protected];   [email protected] 5Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland  [email protected]   Abstracts Background. It is known that in practically healthy (without clinical diagnose) people constellations of principal Adaptation Hormones displays widely variability representing different gradations of health and premorbide states. Previously, we found at least four mutually separated groups of people (endocrine clusters). The aim of this study is to elucidate the characteristics of immunity in members of different neuroendocrine clusters. Material and research methods. We determined twice in ten women and ten men aged 33-76 years without clinical diagnose serum levels of Cortisol, Aldosterone, Triiodothyronine, Testosterone and Calcitonin (ELISA) as well as Bayevskiy’s Stress Index by HRV as markers of General Adaptation Reactions (GAR). Also used determined by Leukocytogram Popovych’s Strain and Adaptation Indices. Immune status evaluated on a set of I and II levels recommended by the WHO. Results. All 40 case was distributed among four neuroendocrine clusters. Cluster N1 contains 10% cases with drastically hypercortisolemia and increased Baevskiy’s Stress Index combined with moderately decreased level of Testosterone but normal levels of Triiodothyronine, Aldosterone and Calcitonin. It is accompanied by a significant supression of cellular immunity and insignificant activation of humoral immunity. 12,5% cases (Cluster N2) characterized moderately increased level of Cortisol and Triiodothyronine combined with moderately decreased level of Calcitonin but normal levels of Testosterone and Aldosterone. Despite the normal average level of Stress Index it ranges in a wide range. This is accompanied by negligible inhibition of cellular immunity and significant activation of humoral immunity. 32,5% cases (Cluster N3) characterized moderately increased level of Cortisol too while moderately decreased level of Triiodothyronine as well as Calcitonin and Testosterone combined with upper borderline level of Aldosterone and normal level of Stress Index. This is accompanied by a significant activation of humoral immunity without abnormalities of cellular immunity. In 45% cases (Cluster N4) neuro-hormonal costellation characterized upper borderline levels of Cortisol as well as Testosterone combined with normal levels others hormones and even slightly reduced level of Stress Index. This is accompanied by more pronounced activation of humoral immunity without abnormalities of cellular immunity. Conclusion. Various constellations of Neuroendocrine factors of General Adaptation Reactions accompanied by various constellations parameters of Immunity. Keywords: Cortisol, Aldosterone, Triiodothyronine, Testosterone, Calcitonin, HRV, Immunity, Relationships