Working Together for Soil Health: Liberating Structures for Participatory Learning in Extension

Liberating Structures (LS) provide a user-friendly toolkit to shift group power dynamics and allow all stakeholders to contribute. We explored the novel use of LS in soil health extension to conduct high-engagement events with diverse stakeholders. Our goals were to promote social learning, networking, and to encourage innovation. Soil health themes emerged highlighting specific practices, and the necessity of addressing broader scope issues of education, economics, and policy. Participants reported increased knowledge of soil health, professional connections, and forecasted participation in soil-health-promoting activities. Participants also expressed a sense of community, expanded perspectives, and appreciation of the co-development process

Analyzing the Implementation of Nutrient Management Plans by Farmers: Implications for Extension Education

We conducted case studies on four Connecticut dairy farms to evaluate how well farmers implemented their nutrient management plans (NMPs). Our findings can help Extension educators develop programs to improve NMPs and NMP adoption by farmers. We identified three educational topic areas that would likely increase NMP understanding and acceptance: (a) soil testing protocol, results interpretation, and nutrient recommendations; (b) manure fertilizer-equivalent value, proper application, and effective distribution; and (c) costs and benefits of substituting on-farm manure for purchased commercial fertilizer. A new adaptive nutrient management program presents a timely opportunity for cost-effective and collaborative on-farm education efforts by Extension and the Natural Resources Conservation Service

Distribution of protein components of wheat from different regions

The distribution of wheat protein components in different regions was researched to provide a theoretical basis on variety selection, quality improvement and food processing. 146 varieties from eight regions were collected to measure contents of protein components (albumin, globulin, gliadin and glutenin) in different regions for the distribution. The largest variation coefficient occurred in contents of globulin, followed by those of gliadin and albumin, while the contents of glutenin varied with the smallest range. The contents of all protein components belonged to normal distribution. It was discovered that the contents of albumin and globulin skewed towards the high value, while glutenin content skewed towards the low value. Differences on the contents of protein components existed in samples from different regions; the regional distribution of four protein components is: the northern region > the southern region > the western region > the eastern region. The contents of protein components of Yannong 19 in different regions were determined, the results displayed that the distributions of four protein components showed the same trends, although the highest contents occurred in Shanxi as compared to the other three regions (Shandong, Jiangsu and An’hui), and there were little differences among them. Geographical conditions can affect the protein components of wheat, and gliadin and glutenin content can affect wheat quality, so we can designate areas where wheat contains more gliadin and glutenin as our high-quality wheat producing areas, of which Shaanxi is a better choice.Keywords: Wheat, protein components, different regions, distributio

Poplar Allene Oxide Synthase 1 Gene Promoter Drives Rapid and Localized Expression by Wounding

Promoters play critical roles in controlling the transcription of genes and are important as tools to drive heterologous expression for biotechnological applications. In addition to core transcription factor-binding motifs that assist in the binding of RNA polymerases, there are specific nucleotide sequences in a promoter region to allow regulation of gene expression. The allene oxide synthase (AOS) gene family are cytochrome P450s that are responsive to a variety of environmental stress, making them good candidates for the discovery of inducible promoters. Populus AOS homologs separate phylogenetically into two clades. Based on the 19 promoter motifs with significant abundance differences between the two clades, Clade I AOS genes are likely more responsive to hormones, salt, and pathogen, whereas clade II homologs are likely inducible by water stress. In this study, an upstream promoter from a Clade I poplar AOS encoding gene (AOS1) was cloned and used to drive the expression of a ß-glucuronidase (GUS) gene in Arabidopsis. AOS is an essential enzyme in the lipoxygenase pathway that is responsible for the production of many nonvolatile oxylipins in plants, including the jasmonates, which are regulatory phytohormones coordinating a variety of biological and stress response functions. Consistent with AOS transcript expression patterns, we found that the poplar AOS1 promoter drives rapid and localized expression by wounding. The study provides insight on the responsive elements in the poplar AOS promoters, but more importantly identifies a strong wound-inducible and localized promoter for future applications. Key Message: Populus AOSs separate phylogenetically into two clades, which show significant abundance differences in 19 promoter motifs. * AOS1 is predominantly expressed in growing vascular tissue in Populus * Populus AOS1 promoter drives rapid and localized expression by wounding in Arabidopsis

Seasonal dynamics of trace elements in sediment and seagrass tissues in the largest Zostera japonica habitat, the Yellow River Estuary, northern China

Trace element accumulation is an anthropogenic threat to seagrass ecosystems, which in turn may affect the health of humans who depend on these ecosystems. Trace element accumulation in seagrass meadows may vary temporally due to, e.g., seasonal patterns in sediment discharge from upstream areas. In addition, when several trace elements are present in sufficiently high concentrations, the risk of seagrass loss due to the cumulative impact of these trace elements is increased. To assess the seasonal variation and cumulative risk of trace element contamination to seagrass meadows, trace element (As, Cd, Cr, Cu, Pb, Hg, Mn and Zn) levels in surface sediment and seagrass tissues were measured in the largest Chinese Zostera japonica habitat, located in the Yellow River Estuary, at three sites and three seasons (fall, spring and summer) in 2014–2015. In all three seasons, trace element accumulation in the sediment exceeded background levels for Cd and Hg. Cumulative risk to Z. japonica habitat in the Yellow River Estuary, from all trace elements together, was assessed as “moderate” in all three seasons examined. Bioaccumulation of trace elements by seagrass tissues was highly variable between seasons and between above-ground and below-ground biomass. The variation in trace element concentration of seagrass tissues was much higher than the variation in trace element concentration of the sediment. In addition, for trace elements which tended to accumulate more in above-ground biomass than below-ground biomass (Cd and Mn), the ratio of above-ground to below-ground trace element concentration peaked at times corresponding to high water discharge and high sediment loads in the Yellow River Estuary. Overall, our results suggest that trace element accumulation in the sediment may not vary between seasons, but bioaccumulation in seagrass tissues is highly variable and may respond directly to trace elements in the water column

Hubble Sinks In The Low-Redshift Swampland

Local determinations of the Hubble constant $H_0$ favour a higher value than Planck based on CMB and $\Lambda$CDM. Through a model-independent expansion, we show that low redshift ($z \lesssim 0.7$) data comprising baryon acoustic oscillations (BAO), cosmic chronometers and Type Ia supernovae has a preference for Quintessence models that lower $H_0$ relative to $\Lambda$CDM. In addition, we confirm that an exponential coupling to dark matter cannot alter this conclusion in the same redshift range. Our results leave open the possibility that a coupling in the matter-dominated epoch, potentially even in the dark ages, may yet save $H_0$ from sinking in the string theory Swampland.Comment: 4 pages; v2 comments, references adde

Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?

Healthy seagrasses are considered a prime indicator of estuarine and coastal ecosystem function; however, as the only group of flowering plants recolonizing the sea, seagrasses are frequently exposed to anthropogenic heavy metal pollutants, which are associated with high levels of molecular damage. To determine whether biologically relevant concentrations of heavy metals cause systematic alterations in RNA expression patterns, we performed a gene expression study using transcriptome analyses (RNA-seq). We exposed the typical intertidal seagrass Zostera japonica to 0 and 50 μM of copper (Cu), lead (Pb), and cadmium (Cd) under laboratory conditions. A total of 18,266 differentially expressed genes (DEGs) were identified, of which 2001 co-expressed genes directly related by Cu, Pb, and Cd stress. We also examined the effects of short-term heavy metal Cu, Pb, and Cd pulses on the accumulation of metals in Z. japonica and showed metal concentrations were higher in the shoots than in roots. Twelve differentially expressed genes were further analyzed for expression differences using real-time quantitative polymerase chain reaction (RT-qPCR). Our data suggest that as coastal seawater pollution worsens, the sensitive genes identified in this study may be useful biomarkers of sublethal effects and provide fundamental information for Z. japonica resistant gene engineering

Genome-wide Association Study Identifies New Loci Associated With Risk Of HBV Infection And Disease Progression

Background: Recent studies have identified susceptibility genes of HBV clearance, chronic hepatitis B, liver cirrhosis, hepatocellular carcinoma, and showed the host genetic factors play an important role in these HBV-related outcomes. Results: In order to discover new susceptibility genes for HBV-related outcomes, we conducted a genome-wide association study in 1031 Chinese participants, including 275 HBV clearance subjects, 92 asymptomatic persistence infection carriers (ASPI), 93 chronic hepatitis B patients (CHB), 188 HBV-related decompensated cirrhosis patients (DC), 214 HBV-related hepatocellular carcinoma patients (HCC) and 169 healthy controls (HC). In the case-control study, we observed novel locus significantly associated with CHB (SNP: rs1264473, Gene: GRHL2, P = 1.57×10-6) and HCC (SNP: rs2833856, Gene: EVA1C, P = 1.62×10-6; SNP: rs4661093, Gene: ETV3, P = 2.26×10-6). In the trend study across progressive stages post HBV infection, one novel locus (SNP: rs1537862, Gene: LACE1, P = 1.85×10-6), and three MHC loci (HLA-DRB1, HLA-DPB1, HLA-DPA2) showed significant increased progressive risk from ASPI to CHB. Interestingly, underlying the evolutionary study of HBV-related genes in public database, we found that the derived allele of two HBV clearance related locus, rs3077 and rs9277542, are under strong selection in European population. Conclusions: In this study, we identified several novel candidate genes associated with individual HBV infectious outcomes, progressive stages, and liver enzymes. Moreover, we identified two SNPs that show selective significance (HLA-DPA1, HLA-DPB1) in non-East Asian (European, American, South Asian) versus East Asian, indicating that host genetic factors contribute to the ethnic disparities of susceptibility of HBV infection. Taken together, these findings provided a new insight into the role of host genetic factors in HBV related outcomes and progression

Moderate relative size of covered and non-covered structures of artificial reef enhances the sheltering effect on reef fish

Identifying the relationship between fish aggregations and artificial reefs (ARs) is important for optimizing reef structures and protecting marine resources subjected to external disturbance. Yet, knowledge remains limited of how the distribution of fish is affected by shelter availability provided by different AR structures. Here, we tested the effects of two structural attributes on the distribution of a benthic juvenile reef fish (fat greenling, Hexagrammos otakii). We used a laboratory mesocosm experiment with a simplified reef unit that was made of covered structure and non-covered structure. The covered structure was defined as the area inside ARs that provided effective shelter. The non-covered structure was defined as the area along the edge of ARs, which attracts fish but has lower sheltering effects. Four scenarios of two orthogonal structural attributes contained in a reef unit were implemented: size of covered structure (small shelter versus large shelter) and size of non-covered structure (small edge versus large edge), forming three size ratios of shelters to edges (low, medium, and high). The sheltering effects of the four scenarios were evaluated based on changes to the distribution patterns of fish under disturbance. We found that the reef with a large shelter had a better sheltering effect than the reef with a small shelter, but was limited by its small edge, especially when fish density was high. In contrast, the sheltering effect of the reef with a small shelter was limited by its large edge compared to the small edge. Thus, a moderate shelter-edge ratio enhanced the ability of juvenile fat greenling to elude external disturbance. Our findings highlight the importance of quantifying how the structural composition of reefs affects fish distributions, providing guidance to optimize AR structures