Complementary ferroelectric-capacitor logic for low-power logic-in-memory VLSI

科研費報告書収録論文(課題番号:13558026・基盤研究(B)(2)・13～16/研究代表者:羽生, 貴弘/転送ボトルネックフリー多値ロジックインメモリVLSIの開発と応用

Retrorectal epidermoid cyst with unusually elevated serum SCC level, initially diagnosed as an ovarian tumor

Retrorectal epidermoid cyst is one of the developmental cysts which arise from remnants of embryonic tissues. We report a rare case of retrorectal epidermoid cyst, initially diagnosed as an ovarian tumor. Serum SCC value as tumor marker was elevated to the high level. Laparoscopy revealed ovaries, uterus and other pelvic organs were all normal. This tumor existed in the retroperitoneal cavity and compressed the rectum. Later, complete tumor resection was performed by laparotomy. Histological study revealed the epithelium of this tumor consisted of only squamous cells without atypia, and the diagnosis of this tumor was retrorectal epidermoid cyst. Retrorectal epidermoid cyst is very rare, and difficult to diagnose before surgery. However, if we have-knowledge of developmental cysts, and by careful digital examination and image diagnosis, a differential diagnosis can be made

Ferroelectric-based functional pass-gate for fine-grain pipelined VLSI computation

科研費報告書収録論文(課題番号:13558026・基盤研究(B)(2)・13～16/研究代表者:羽生, 貴弘/転送ボトルネックフリー多値ロジックインメモリVLSIの開発と応用

Isolated gestational proteinuria preceding the diagnosis of preeclampsia : an observational study

Introduction. Some pregnant women develop significant proteinuria in the absence of hypertension. However, clinical significance of isolated gestational proteinuria (IGP) is not well understood. This study aimed to determine the prevalence of IGP in singleton pregnancies and the proportion of women with IGP who subsequently developed preeclampsia (IGP-PE) among all PE cases. Material and methods. This was an observational study of 6819 women with singleton pregnancies at 12 centers, including 938 women with at least once determination of protein-to-creatinine ratio (P/Cr). Significant proteinuria in pregnancy (SPIP) was defined as P/Cr (mg/mg) level >0.27. IGP was defined as SPIP in the absence of hypertension. Gestational hypertension (GH) preceding preeclampsia (GH-PE) was defined as preeclampsia (PE) in which GH preceded SPIP. Simultaneous PE (S-PE) was defined as PE in which both SPIP and hypertension occurred simultaneously. Results. IGP and PE were diagnosed in 130 (1.9%) and 158 (2.3%) of 6819 women, respectively. Of 130 women with IGP, 32 (25%) progressed to PE and accounted for 20% of all women with PE. Hence, women with IGP had a relative risk of 13.1 (95% CI; 9.2-18.5) for developing PE compared with those without IGP [25% (32/130) vs. 1.9% (126/6689)]. At diagnosis of SPIP, P/Cr levels already exceeded 1.0 more often in women with S-PE than in those with IGP-PE [67% (33/49) vs. 44% (14/32), respectively, p = 0.031]. Conclusions. IGP is a risk factor for PE, and IGP-PE accounts for a considerable proportion (20%) of all PE

Localization of Stem Cells in Small Intestinal Epithelium:Strategies for Identifying Small Intestinal Stem Cells

In the small intestine, stem cells are considered to exist at the bottom of the crypt. Actively proliferatingtransitional cells supplied from stem cells are differentiated into two directions upward and downward. Upwardcells are differentiated into absorbing epithelial cells, goblet cells, and endocrine cells, and downwardcells differentiated into Paneth cells. However there are some difficulties to identify the stem cells becauseof their unique characteristics. At first, stem cells occur as actual stem cells and potential stem cells, andsecond, there is diversity in stem cells. Therefore, molecules suitable for a marker of small intestinal stemcells are necessary to distinguish "true" stem cells from others. Energetically searched for in recent years,Musashi-1, type 1A bone morphogenetic protein receptor (BMPR-1A), phospho-phosphatase and tensinhomolog deleted on chromosome ten( phospho-PTEN), doublecortin and calmodulin kinase-like-1( DCAMKL1),ephrin receptors( Eph receptors), integrins, and leucine-rich repeat-containing G protein-coupled receptor5 (Lgr5) are proposed. Among them, Musashi-1 draws attention as one of a candidate marker forsmall intestinal stem cells. We here introduce our reviews about expression of Musashi-1 and Hes1 proteinsin the small intestine, and would like to overview the way to identify the small intestinal stem cells

FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche

Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germline niche by providing these factors using a drug delivery system in vivo. Although both factors expanded the GFRA1+ subset of undifferentiated spermatogonia, the FGF2-expanded subset expressed RARG, which is indispensable for proper differentiation, 1.9-fold more frequently than the GDNF-expanded subset, demonstrating that FGF2 expands a differentiation-prone subset in the testis. Moreover, FGF2 acted on the germline niche to suppress RA metabolism and GDNF production, suggesting that FGF2 modifies germline niche functions to be more appropriate for spermatogonial differentiation. These results suggest that FGF2 contributes to induction of differentiation rather than maintenance of undifferentiated spermatogonia, indicating reconsideration of the role of FGF2 in the germline niche

ケイチョクガタ ノウセイ マヒ ニヨル シタイ フジユウシャ ニオケル ダツ トレーニング ノ エイキョウ

It is very important to continue the muscle training for the crippled due to spastic cerebral palsy. By this research, we gave the experiment of isokinetic training by CYBEX770 on both of knee joints, both of hip joints and muscle training by free weight on the crippled due to spastic cerebral palsy on a daily life basis for one and a half years within detraining period. As a result, unexpectedly in training period, in detraining period, there were significant differences regarding some more items in muscle strength of the knee joints than in muscle strength of the hip joints. There is not a decline in effect of detraining but rather effectiveness and it is confirmed that the detraining had an influence more on muscle strength of the hip joints than on muscle strength of the knee joints which are slightly symptomatic part of the crippled test subject due to spastic cerebral palsy. It is necessary to be inspected about the influence of the detraining period

ジキュウテキ ウンドウジ ノ ダエキチュウ α-アミラーゼ カッセイチ ノ ヘンドウ ヨウイン

In this study, we examined usefulness of α−AMY activity value in saliva as a non−invasive stress marker in the enduring exercise. In experiment 1, nine high school students participated in the measurement of the day−to−day variation and diurnal variation of saliva α−AMY activity value at rest. In experiment 2, seven adult male athletes participated in the measurement of α−AMY activity value in saliva during gradually increased or decreased exercise test. In Experiment 3, two adult males of identical twins and fraternal twins who specialize in long−distance run, we verified the constitutional impact on α−AMY activity value change in saliva during gradually increased or decreased exercise test. Summaries of knowledge obtained from these experiments are as follows. 1．A day change of α−AMY activity value in saliva at rest showed transition reflecting circadian rhythm. 2．Diurnal change for four days of α−AMY activity value in saliva at rest didn’t show significant change. 3．A strongly positive correlation was observed between α−AMY activity value in saliva and heart rate in endurance exercise. 4．The α−AMY activity value in saliva during endurance exercise changed reflecting the exercise intensity, but large individual differences were also observed. 5．From the ups and downs of α−AMY activity value in saliva of identical twins during endurance exercise, constitutional influence was little suggested. From the above results, α−AMY activity value in saliva was suggested to be useful as a non−invasive stress marker in enduring exercise. However, the change is observed to correspond to individual differences, and personal factors such as physical condition and characteristics of the subject seem to be related to, which will be the future work to verify

Sessile serrated adenoma/polyp-cancer sequenceにおいてS100Pは遺伝子脱メチル化により発現し、細胞増殖を促進する

Background/Aims: Sessile serrated adenomas/polyps (SSA/Ps) are a putative precursor lesion of colon cancer. Although the relevance of DNA hypermethylation in the SSA/P-cancer sequence is well documented, the role of DNA hypomethylation is unknown. We investigated the biological relevance of DNA hypomethylation in the SSA/P-cancer sequence by using 3-dimensional organoids of SSA/P. Methods: We first analyzed hypomethylated genes using datasets from our previous DNA methylation array analysis on 7 SSA/P and 2 cancer in SSA/P specimens. Expression levels of hypomethylated genes in SSA/P specimens were determined by RT-PCR and immunohistochemistry. We established 3-dimensional SSA/P organoids and performed knockdown experiments using a lentiviral shRNA vector. DNA hypomethylation at CpG sites of the gene was quantitated by MassARRAY analysis. Results: The mean number of hypomethylated genes in SSA/P and cancer in SSA/P was 41.6 ± 27.5 and 214 ± 19.8, respectively, showing a stepwise increment in hypomethylation during the SSA/P-cancer sequence. S100P, S100α2, PKP3, and MUC2 were most commonly hypomethylated in SSA/P specimens. The mRNA and protein expression levels of S100P, S100α2, and MUC2 were significantly elevated in SSA/P compared with normal colon tissues, as revealed by RT-PCR and immunohistochemistry, respectively. Among these, mRNA and protein levels were highest for S100P. Knockdown of the S100P gene using a lentiviral shRNA vector in 3-dimensional SSA/P organoids inhibited cell growth by >50% (p < 0.01). The mean diameter of SSA/P organoids with S100P gene knockdown was significantly smaller compared with control organoids. MassARRAY analysis of DNA hypomethylation in the S100P gene revealed significant hypomethylation at specific CpG sites in intron 1, exon 1, and the 5′-flanking promoter region. Conclusion: These results suggest that DNA hypomethylation, including S100P hypomethylation, is supposedly associated with the SSA/P-cancer sequence. S100P overexpression via DNA hypomethylation plays an important role in promoting cell growth in the SSA/P-cancer sequence