592 research outputs found
Redirecting a Scattered Public Toward Alternative Matters of Concern: Shifting Perceptions of Urban Wastewater Governance in Indonesia
Designers can and already do play an important role in supporting publics as they are coming into being and to help clarify and express the publics interests. The coming into being of a public, however, does not guarantee it acts in its own best interest or in the best interest of the common good. Publics existing matters-of-concern might, as demonstrated in this article, be influenced by deeply engrained societal norms and values, or even fear for demonstrating other concerns. What is the role of the designer in such situations? In this article I suggest designers can engage in redirecting publics towards alternative matters-of-concern
Incorporating double copies of a chromatin insulator into lentiviral vectors results in less viral integrants
<p>Abstract</p> <p>Background</p> <p>Lentiviral vectors hold great promise as gene transfer vectors in gene therapeutic settings. However, problems related to the risk of insertional mutagenesis, transgene silencing and positional effects have stalled the use of such vectors in the clinic. Chromatin insulators are boundary elements that can prevent enhancer-promoter interactions, if placed between these elements, and protect transgene cassettes from silencing and positional effects. It has been suggested that insulators can improve the safety and performance of lentiviral vectors. Therefore insulators have been incorporated into lentiviral vectors in order to enhance their safety profile and improve transgene expression. Commonly such insulator vectors are produced at lower titers than control vectors thus limiting their potential use.</p> <p>Results</p> <p>In this study we cloned in tandem copies of the chicken β-globin insulator (cHS4) on both sides of the transgene cassette in order to enhance the insulating effect. Our insulator vectors were produced at significantly lower titers compared to control vectors, and we show that this reduction in titer is due to a block during the transduction process that appears after reverse transcription but before integration of the viral DNA. This non-integrated viral DNA could be detected by PCR and, importantly, prevented efficient transduction of target cells.</p> <p>Conclusion</p> <p>These results have importance for the future use of insulator sequences in lentiviral vectors and might limit the use of insulators in vectors for <it>in vivo </it>use. Therefore, a careful analysis of the optimal design must be performed before insulators are included into clinical lentiviral vectors.</p
How to design governance for lasting service? Visual resource for workshop, guided stakeholder discussion and group/individual reflection
Small Disks and Semiclassical Resonances
We study the effect on quantum spectra of the existence of small circular
disks in a billiard system. In the limit where the disk radii vanish there is
no effect, however this limit is approached very slowly so that even very small
radii have comparatively large effects. We include diffractive orbits which
scatter off the small disks in the periodic orbit expansion. This situation is
formally similar to edge diffraction except that the disk radii introduce a
length scale in the problem such that for wave lengths smaller than the order
of the disk radius we recover the usual semi-classical approximation; however,
for wave lengths larger than the order of the disk radius there is a
qualitatively different behaviour. We test the theory by successfully
estimating the positions of scattering resonances in geometries consisting of
three and four small disks.Comment: Final published version - some changes in the discussion and the
labels on one figure are correcte
Spectral statistics in chaotic systems with a point interaction
We consider quantum systems with a chaotic classical limit that are perturbed
by a point-like scatterer. The spectral form factor K(tau) for these systems is
evaluated semiclassically in terms of periodic and diffractive orbits. It is
shown for order tau^2 and tau^3 that off-diagonal contributions to the form
factor which involve diffractive orbits cancel exactly the diagonal
contributions from diffractive orbits, implying that the perturbation by the
scatterer does not change the spectral statistic. We further show that
parametric spectral statistics for these systems are universal for small
changes of the strength of the scatterer.Comment: LaTeX, 21 pages, 7 figures, small corrections, new references adde
RHYTHM-AF: design of an international registry on cardioversion of atrial fibrillation and characteristics of participating centers
BACKGROUND Atrial fibrillation is a serious public health problem posing a considerable burden to not only patients, but the healthcare environment due to high rates of morbidity, mortality, and medical resource utilization. There are limited data on the variation in treatment practice patterns across different countries, healthcare settings and the associated health outcomes. METHODS/DESIGN RHYTHM-AF was a prospective observational multinational study of management of recent onset atrial fibrillation patients considered for cardioversion designed to collect data on international treatment patterns and short term outcomes related to cardioversion. We present data collected in 10 countries between May 2010 and June 2011. Enrollment was ongoing in Italy and Brazil at the time of data analysis. Data were collected at the time of atrial fibrillation episode in all countries (Australia, Brazil, France, Germany, Italy, Netherlands, Poland, Spain, Sweden, United Kingdom), and cumulative follow-up data were collected at day 60 (±10) in all but Spain. Information on center characteristics, enrollment data, patient demographics, detail of atrial fibrillation episode, medical history, diagnostic procedures, acute treatment of atrial fibrillation, discharge information and the follow-up data on major events and rehospitalizations up to day 60 were collected. DISCUSSIN A total of 3940 patients were enrolled from 175 acute care centers. 70.5% of the centers were either academic (44%) or teaching (26%) hospitals with an overall median capacity of 510 beds. The sites were mostly specialized with anticoagulation clinics (65.9%), heart failure (75.1%) and hypertension clinics (60.1%) available. The RHYTHM-AF registry will provide insight into regional variability of antiarrhythmic and antithrombotic treatment of atrial fibrillation, the appropriateness of such treatments with respect to outcomes, and their cost-efficacy. Observations will help inform strategies to improve cardiovascular outcomes in patients with atrial fibrillation. TRIAL REGISTRATION Clinical trials NCT01119716Harry JGM Crijns, Lori D Bash, François Chazelle, Jean-Yves Le Heuzey, Thorsten Lewalter, Gregory YH Lip, Aldo P Maggioni, Alfonso Martín, Piotr Ponikowski, Mårten Rosenqvist, Prashanthan Sanders, Mauricio Scanavacca, Alexandra A Bernhardt, Sreevalsa Unniachan, Hemant M Phatak and Anselm K Git
First results on Martian carbon monoxide from Herschel/HIFI observations
We report on the initial analysis of Herschel/HIFI carbon monoxide (CO)
observations of the Martian atmosphere performed between 11 and 16 April 2010.
We selected the (7-6) rotational transitions of the isotopes ^{13}CO at 771 GHz
and C^{18}O at 768 GHz in order to retrieve the mean vertical profile of
temperature and the mean volume mixing ratio of carbon monoxide. The derived
temperature profile agrees within less than 5 K with general circulation model
(GCM) predictions up to an altitude of 45 km, however, show about 12-15 K lower
values at 60 km. The CO mixing ratio was determined as 980 \pm 150 ppm, in
agreement with the 900 ppm derived from Herschel/SPIRE observations in November
2009.Comment: Accepted for publication in Astronomy and Astrophysics (special issue
on HIFI first results); minor changes to match published versio
Edge Diffraction, Trace Formulae and the Cardioid Billiard
We study the effect of edge diffraction on the semiclassical analysis of two
dimensional quantum systems by deriving a trace formula which incorporates
paths hitting any number of vertices embedded in an arbitrary potential. This
formula is used to study the cardioid billiard, which has a single vertex. The
formula works well for most of the short orbits we analyzed but fails for a few
diffractive orbits due to a breakdown in the formalism for certain geometries.
We extend the symbolic dynamics to account for diffractive orbits and use it to
show that in the presence of parity symmetry the trace formula decomposes in an
elegant manner such that for the cardioid billiard the diffractive orbits have
no effect on the odd spectrum. Including diffractive orbits helps resolve peaks
in the density of even states but does not appear to affect their positions. An
analysis of the level statistics shows no significant difference between
spectra with and without diffraction.Comment: 25 pages, 12 Postscript figures. Published versio
Recommendations for Next‐Generation Ground Magnetic Perturbation Validation
Data‐model validation of ground magnetic perturbation forecasts, specifically of the time rate of change of surface magnetic field, dB/dt, is a critical task for model development and for mitigation of geomagnetically induced current effects. While a current, community‐accepted standard for dB/dt validation exists (Pulkkinen et al., 2013), it has several limitations that prevent more complete understanding of model capability. This work presents recommendations from the International Forum for Space Weather Capabilities Assessment Ground Magnetic Perturbation Working Team for creating a next‐generation validation suite. Four recommendations are made to address the existing suite: greatly expand the number of ground observatories used, expand the number of events included in the suite from six to eight, generate metrics as a function of magnetic local time, and generate metrics as a function of activity type. For each of these, implementation details are explored. Limitations and future considerations are also discussed.Plain Language SummarySpace weather forecast models of magnetic field perturbations are important for protecting the power grid and other vulnerable infrastructure. These models must be validated by comparing their predictions to observations. This paper makes recommendations for how future models should be validated in order to best test their capabilities.Key PointsWe present a new validation suite for models of ground magnetic perturbations, dB/dt, of interest for geomagnetically induced currentsThe existing standard remains useful but provides limited information, so an expanded set of metrics is defined hereThis work is a result of the International Forum for Space Weather Capabilities Assessment and represents a new community consensusPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147786/1/swe20777_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147786/2/swe20777.pd
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