Glossophobia: Fear of Public Speaking Among Senior High School Students of Aliaga National High School

This study entitled “Glossophobia: Fear of Public Speaking Among Grade 11 Senior High School Students in Aliaga National High School” aimed to describe and analyze the components that contribute to the glossophobia among Grade 11 Senior High School students. Furthermore, it sought to determine the significant difference in the components to glossophobia among the students in terms of their profile. It also aimed to create interventions that can be applied to address challenges and issues on gloosophobia that the students encountered during public speaking class

The RNA Domain Vc1 Regulates Downstream Gene Expression in Response to Cyclic Diguanylate in Vibrio cholerae

In many bacterial species, including the aquatic bacterium and human pathogen Vibrio cholerae, the second messenger cyclic diguanylate (c-di-GMP) modulates processes such as biofilm formation, motility, and virulence factor production. By interacting with various effectors, c-di-GMP regulates gene expression or protein function. One type of c-di-GMP receptor is the class I riboswitch, representatives of which have been shown to bind c-di-GMP in vitro. Herein, we examined the in vitro and in vivo function of the putative class I riboswitch in Vibrio cholerae, Vc1, which lies upstream of the gene encoding GbpA, a colonization factor that contributes to attachment of V. cholerae to environmental and host surfaces containing N-acetylglucosamine moieties. We provide evidence that Vc1 RNA interacts directly with c-di-GMP in vitro, and that nucleotides conserved among this class of riboswitch are important for binding. Yet the mutation of these conserved residues individually in the V. cholerae chromosome inconsistently affects the expression of gbpA and production of the GbpA protein. By isolating the regulatory function of Vc1, we show that the Vc1 element positively regulates downstream gene expression in response to c-di-GMP. Together these data suggest that the Vc1 element responds to c-di-GMP in vivo. Positive regulation of gbpA expression by c-di-GMP via Vc1 may influence the ability of V. cholerae to associate with chitin in the aquatic environment and the host intestinal environment

Hypermedia-based tutoring: methodology for the production of hypermedia resources through face-to-face tutoring

We present a methodology for creating hypermedia materials derived from face-to-face tutoring sessions between a tutor and graduate students. To create the hypermedia materials, the tutor and the student used a smart pen which allowed to record the conversation and digitalize the notes being taken. The production of hypermedia material is based on the use of visual representations and text to help students go from concrete to abstract thinking and vice versa. We point out that hypermedia materials are audio-visual narratives (i.e., dynamic graphics, diagrams) that facilitate the representation of co-constructed shared knowledge and let participants navigate between oral and textual information. This methodology allows the production of individualized material without investing additional time in editing and designing. The hypermedia based tutoring (HBT) model is highly valued by students since it helps them to go over the discussions with the tutor and review the thinking process that both constructed during the session. HBT becomes a creative form of communicating and representing information that challenges the tutor and student to develop new skills and ways of thinking. The model that we propose here requires to change traditional tutor and student roles and to create learning experiences that do not overlook students’ needsThe eMadrid Excellence Network is funded by Madrid Regional Government (Comunidad de Madrid) grant no. P2013/ICE-2715. The FOMIX grant No. MOR-2013-C01-225102 is funded by Fondo Mixto-CONACYT (Morelos State Government and the Federal Agency CONACYT, Mexico

Cumulants of the three state Potts model and of nonequilibrium models with C3v symmetry

The critical behavior of two-dimensional stochastic lattice gas models with C3v symmetry is analyzed. We study the cumulants of the order parameter for the three state (equilibrium) Potts model and for two irreversible models whose dynamic rules are invariant under the symmetry operations of the point group C3v. By means of extensive numerical analysis of the phase transition we show that irreversibility does not affect the critical behavior of the systems. In particular we find that the Binder reduced fourth order cumulant takes a universal value U* which is the same for the three state Potts model and for the irreversible models. The same universal behavior is observed for the reduced third-order cumulant.Comment: gzipped tar file containing: 1 latex file + 6 eps figure

Two nucleotide second messengers regulate the production of the Vibrio cholerae colonization factor GbpA

Abstract Background The nucleotide second messengers cAMP and c-di-GMP allow many bacteria, including the human intestinal pathogen Vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. In response to limitation of specific carbohydrates, cAMP and its receptor CRP control the transcription of genes important for nutrient acquisition and utilization; c-di-GMP controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional mechanisms. In this study, we investigated the convergence of cAMP and c-di-GMP signaling pathways in regulating the expression of gbpA. GbpA is a colonization factor that participates in the attachment of V. cholerae to N-acetylglucosamine-containing surfaces in its native aquatic environment and the host intestinal tract. Results We show that c-di-GMP inhibits gbpA activation in a fashion independent of the known transcription factors that directly sense c-di-GMP. Interestingly, inhibition of gbpA activation by c-di-GMP only occurs during growth on non-PTS dependent nutrient sources. Consistent with this result, we show that CRP binds to the gbpA promoter in a cAMP-dependent manner in vitro and drives transcription of gbpA in vivo. The interplay between cAMP and c-di-GMP does not broadly impact the CRP-cAMP regulon, but occurs more specifically at the gbpA promoter. Conclusions These findings suggest that c-di-GMP directly interferes with the interaction of CRP-cAMP and the gbpA promoter via an unidentified regulator. The use of two distinct second messenger signaling mechanisms to regulate gbpA transcription may allow V. cholerae to finely modulate GbpA production, and therefore colonization of aquatic and host surfaces, in response to discrete environmental stimuli

Mean Field Behavior of Cluster Dynamics

The dynamic behavior of cluster algorithms is analyzed in the classical mean field limit. Rigorous analytical results below $T_c$ establish that the dynamic exponent has the value $z_{sw}=1$ for the Swendsen-Wang algorithm and $z_{uw}=0$ for the Wolff algorithm. An efficient Monte Carlo implementation is introduced, adapted for using these algorithms for fully connected graphs. Extensive simulations both above and below $T_c$ demonstrate scaling and evaluate the finite-size scaling function by means of a rather impressive collapse of the data.Comment: Revtex, 9 pages with 7 figure

Driven diffusive system with non-local perturbations

We investigate the impact of non-local perturbations on driven diffusive systems. Two different problems are considered here. In one case, we introduce a non-local particle conservation along the direction of the drive and in another case, we incorporate a long-range temporal correlation in the noise present in the equation of motion. The effect of these perturbations on the anisotropy exponent or on the scaling of the two-point correlation function is studied using renormalization group analysis.Comment: 11 pages, 2 figure

Over-expression of Thioredoxin-1 mediates growth, survival, and chemoresistance and is a druggable target in diffuse large B-cell lymphoma

Diffuse Large B cell lymphomas (DLBCL) are the most prevalent of the non-Hodgkin lymphomas and are currently initially treated fairly successfully, but frequently relapse as refractory disease, resulting in poor salvage therapy options and short survival. The greatest challenge in improving survival of DLBCL patients is overcoming chemo-resistance, whose basis is poorly understood. Among the potential mediators of DLBCL chemo-resistance is the thioredxoin (Trx) family, primarily because Trx family members play critical roles in the regulation of cellular redox homeostasis, and recent studies have indicated that dysregulated redox homeostasis also plays a key role in chemoresistance. In this study, we showed that most of the DLBCL-derived cell lines and primary DLBCL cells express higher basal levels of Trx-1 than normal B cells and that Trx-1 expression level is associated with decreased patients survival. Our functional studies showed that inhibition of Trx-1 by small interfering RNA or a Trx-1 inhibitor (PX-12) inhibited DLBCL cell growth, clonogenicity, and also sensitized DLBCL cells to doxorubicin-induced cell growth inhibition in vitro. These results indicate that Trx-1 plays a key role in cell growth and survival, as well as chemoresistance, and is a potential target to overcome drug resistance in relapsed/refractory DLBCL

Effects of multicomponent and power training programs using elastic devices on motor function, body composition, and metabolic, bone and inflammatory profile in older adults

Background: It is needed to understand what type of training strategy can be the most effective for contributing to a healthier, active, and more independent elderly population. Nowadays, there are novel types of training interventions and devices, but only little is known regarding whether these can provoke positive benefits in this target population. Concretely, no evidence has examined the effectiveness of high-speed resistance training and multicomponent training in older adults in respect of not only physical function but also bone, immunity, and metabolic status. Developing an understanding these novel training strategies can ultimately provide a viable alternative to traditional modes of exercise training for a broader range of participants