8 research outputs found
Parallel regulation of thyroid hormone transporters OATP1c1 and MCT8 during and after endotoxemia at the blood-brain barrier of male rodents
There is increasing evidence that local thyroid hormone (TH) availability changes profoundly in inflammatory conditions due to altered expression of deiodinases that metabolize TH. It is largely unknown, however, how inflammation affects TH availability via the expression of TH transporters. In this study we examined the effect of bacterial lipopolysaccharide (LPS) administration on two TH transporters that are critically important for brain TH homeostasis, organic anion-transporting polypeptide 1c1 (OATP1c1) and monocarboxylate transporter 8 (MCT8). Messenger RNA levels were studied by in situ hybridization and quantitative PCR, and protein levels by immunofluorescence in both the rat and mouse forebrain. The mRNA of both transporters decreased robustly in the first 9h after LPS injection, selectively in brain blood vessels; OATP1c1 mRNA in astrocytes and MCT8 mRNA in neurons remained unchanged. At 24 and/or 48h after LPS administration, OATP1c1 and MCT8 mRNAs increased markedly above control levels in brain vessels. OATP1c1 protein decreased markedly in vessels by 24h, whereas MCT8 protein levels did not decrease significantly. These changes were highly similar in mice and rats. The data demonstrate that OATP1c1 and MCT8 expression are regulated in a parallel manner during inflammation at the blood-brain barrier of rodents. Given the indispensable role of both transporters in allowing TH access to the brain, the results suggest reduced brain TH uptake during systemic inflammation
Pathology and glia type specific changes of the DPP4 activity in the spinal cord contributes to the development and maintenance of hyperalgesia and shapes opioid signalling in chronic pain states
Altered pain sensations such as hyperalgesia and allodynia are characteristic features of various chronic pain states, and remain difficult to treat. We have shown previously that spinal application of dipeptidyl peptidase 4 (DPP4) enzyme inhibitors results in a strong antihyperalgesic effect during inflammatory pain states. In this study we observed a low level of mRNA for DPP4 in the rat spinal dorsal horn in physiological conditions, which did not change significantly either in carrageenan-induced inflammatory or partial nerve ligation-generated neuropathic states. Although DPP4 protein was detected in neurons, astrocytes and microglia in naĂŻve animals its expression significantly increased in astrocytes during inflammation and in microglia in neuropathic conditions. Intrathecal application of two DPP4 inhibitors the tripeptide isoleucin-prolin-isoleucin (IPI) and the antidiabetic drug vildagliptin resulted in robust opioid-dependent antihyperalgesic effect during inflammation and an opioid-independent effect in the Seltzer model. The opioid-mediated antihyperalgesic effect of IPI was exclusively related to mu-opioid receptors, while vildagliptin affected mainly delta-receptor activity, although mu- and kappa-receptors were also involved.
Our results suggest a pathology and glia type specific changes of the DPP4 activity in the spinal cord which contributes to the development and maintenance of hyperalgesia and shapes opioid signalling
Aspergillus fumigatus által termelt extracelluláris triacetil-fuzarinin C és intracelluláris ferrikrocin szideroforok kihozatalának optimalizálása
Aspergillus fumigatus által termelt extracelluláris triacetil-fuzarinin C és intracelluláris ferrikrocin szideroforok kihozatalának optimalizálásaMSc/MABiotechnológiag
Bioprotekcióra alkalmas mikroorganizmusok izolálása
A bioprotekció az utóbbi évtizedben kifejlıdött tudományág, melynek jelentısége
napjainkban egyre növekszik. A gyümölcsök szüret utáni védelme problémát jelent egyrészt a
penészfajok fungicid rezisztencia miatti csökkenı hatékonysága, másrészt a fungicidek
felhasználását ellenzı Ă©s a szintetikus peszticidek elıállĂtásának beszĂĽntetĂ©sĂ©t sĂĽrgetı
társadalmi nyomás miatt. Az egyik lehetséges megoldás a fenti problémákra az antifungális
tulajdonságokkal bĂrĂł mikroorganizmusok használata bioprotektĂv ágenskĂ©nt, melyek
csökkentik, vagy meggátolják a destruktĂv gombák fejlıdĂ©sĂ©t a tárolás során. Az antagonista
élesztık különös figyelmet kaptak, mivel mőködésük rendszerint nem függ össze
antibiotikumok, vagy más, toxikus anyagcsere termékek termelésével. A Candida,
Cryptococcus, Debaromyces, Metschnikowia, Pichia, Rhodotorula, Sporobolomyces, Ă©s
Trichosporon fajok mindegyike rendelkezik gyümölcsök postharvest védelmére alkalmas
tulajdonságokkal antifungális hatásuk révén. (M. Sipiczki, 2006)
Célom éppen ezért biokontrolra, ezen belül antagonizmusra képes mikroorganizmusok
izolálása volt gyĂĽmölcsrıl, az Ă©n esetemben biokertĂ©szetbıl származĂł Elstar tĂpusĂş (16/D)
alma felületérıl. Továbbá az izolált mikroorganizmusok antagonista tulajdonságának
kimutatása az alma károsodását leggyakrabban okozó Penicillium expansum valamint a
Botrytis cinera penĂ©szfajokkal szemben. Hazánkban kĂĽlönös jelentısĂ©ggel bĂr a nagy mĂşltĂş
gyümölcskultúrák védelme. Kézenfekvınek mutatkozik tehát, a tájegység jellemzı
gyümölcsének, az almának kiválasztása vizsgálatunk alanyául.Bag
Ökotoxikológiai vizsgálatok kunszentmártoni krómmal szennyezett mintaterületen
MintavĂ©teli terĂĽletem a Pannonia Rt. már nem működĹ‘ kunszentmártoni bĹ‘rkikĂ©szĂtĹ‘ Ă©s bĹ‘rfeldolgozĂł gyárának szennyvĂzĂĽlepĂtĹ‘ tĂłrendszere volt, amit 17 Ă©vig használtak krĂłmtartalmĂş szennyvĂz, majd szennyvĂziszap elhelyezĂ©sĂ©re. Kutatásom cĂ©lja a terĂĽlet ökotoxikolĂłgiai vizsgálata.MscKörnyezettudományg
Phytoremediation and Ecotoxicological Investigation of Chromium Contaminated Area at Kunszentmárton in Hungary.
Optimization of triacetylfusarinine C and ferricrocin productions in Aspergillus fumigatus
Optimization of triacetylfusarinine C and ferricrocin productions in Aspergillus fumigatus
Iron is an essential element for all microorganisms. Bacteria and fungi produce versatile siderophores for binding and storing this essential transition metal when its availability is limited in the environment. The aim of the study was to optimize the fermentation medium of Aspergillus fumigatus for siderophore production. Triacetyl-fusarinine C and ferricrocin yields were dependent on glucose and glycine supplementations as well as the initial pH of the culture media. The optimal fermentation medium for triacetylfusarinine C production contained 8% glucose, 0.4% glycine and the initial pH was set to 5.9. Meanwhile, maximal ferricrocin yields were recorded in the presence of 10% glucose, 0.5% glycine and at an initial pH of 7.4. Under optimized fermentation conditions, the yields for triacetylfusarinine C and ferricrocin increased up to 2.9 g/l culture medium and 18.9 mg/g mycelium, respectively