Achieving a wide field near infrared camera for the Calar Alto 3.5m telescope

The ongoing development of large infrared array detectors has enabled wide field, deep surveys to be undertaken. There are, however, a number of challenges in building an infrared instrument which has both excellent optical quality and high sensitivity over a wide field. We discuss these problems in the context of building a wide field imaging camera for the 3.5m telescope at Calar Alto with the new 2K*2K HgCdTe HAWAII-2 focal plane array. Our final design is a prime focus camera with a 15' field-of-view, called Omega 2000. To achieve excellent optical quality over the whole field, we have had to dispense with the reimaging optics and cold Lyot stop. We show that creative baffling schemes, including the use of undersized baffles, can compensate for the lost K band sensitivity. A moving baffle will be employed in Omega 2000 to allow full transmission in the non-thermal J and H bands.Comment: To appear in the SPIE proceedings of ``Optical and IR Telescope Instrumentation and Detectors'', Munich, March 200

Macrophage Replication Screen Identifies a Novel Francisella Hydroperoxide Resistance Protein Involved in Virulence

Francisella tularensis is a Gram-negative facultative intracellular pathogen and the causative agent of tularemia. Recently, genome-wide screens have identified Francisella genes required for virulence in mice. However, the mechanisms by which most of the corresponding proteins contribute to pathogenesis are still largely unknown. To further elucidate the roles of these virulence determinants in Francisella pathogenesis, we tested whether each gene was required for replication of the model pathogen F. novicida within macrophages, an important virulence trait. Fifty-three of the 224 genes tested were involved in intracellular replication, including many of those within the Francisella pathogenicity island (FPI), validating our results. Interestingly, over one third of the genes identified are annotated as hypothetical, indicating that F. novicida likely utilizes novel virulence factors for intracellular replication. To further characterize these virulence determinants, we selected two hypothetical genes to study in more detail. As predicted by our screen, deletion mutants of FTN_0096 and FTN_1133 were attenuated for replication in macrophages. The mutants displayed differing levels of attenuation in vivo, with the FTN_1133 mutant being the most attenuated. FTN_1133 has sequence similarity to the organic hydroperoxide resistance protein Ohr, an enzyme involved in the bacterial response to oxidative stress. We show that FTN_1133 is required for F. novicida resistance to, and degradation of, organic hydroperoxides as well as resistance to the action of the NADPH oxidase both in macrophages and mice. Furthermore, we demonstrate that F. holarctica LVS, a strain derived from a highly virulent human pathogenic species of Francisella, also requires this protein for organic hydroperoxide resistance as well as replication in macrophages and mice. This study expands our knowledge of Francisella's largely uncharacterized intracellular lifecycle and demonstrates that FTN_1133 is an important novel mediator of oxidative stress resistance

Design and implementation of a service-oriented driver architecture for LINC-NIRVANA

LINC-NIRVANA (LN) is a German-Italian Fizeau (imaging) interferometer for the Large Binocular Telescope (LBT). The Instrument Control Software (ICS) of this instrument is a hierarchical, distributed software package, which runs on several computers. In this paper we present the bottom layer of the hierarchy - the Basic Device Application (BASDA) layer. This layer simplifies the development of the ICS through a general driver architecture, which supports different types of hardware. This generic device architecture provides a high level interface to encapsulate the hardware dependent driver. The benefit of such a device architecture is to keep the basic device-driver layer flexible and independent from the hardware, and to keep the hardware transparent to the ICS. Additionally, the basic device-driver layer supports interfaces to IDL based applications for calibration and laboratory testing of astronomical instruments, and interfaces to engineering GUIs that allow to maintain the software components easily

Changes in Gut Microbiota after a Four-Week Intervention with Vegan vs. Meat-Rich Diets in Healthy Participants: A Randomized Controlled Trial

An essential role of the gut microbiota in health and disease is strongly suggested by recent research. The composition of the gut microbiota is modified by multiple internal and external factors, such as diet. A vegan diet is known to show beneficial health effects, yet the role of the gut microbiota is unclear. Within a 4-week, monocentric, randomized, controlled trial with a parallel group design (vegan (VD) vs. meat-rich (MD)) with 53 healthy, omnivore, normal-weight participants (62% female, mean 31 years of age), fecal samples were collected at the beginning and at the end of the trial and were analyzed using 16S rRNA gene amplicon sequencing (Clinical Trial register: DRKS00011963). Alpha diversity as well as beta diversity did not differ significantly between MD and VD. Plotting of baseline and end samples emphasized a highly intra-individual microbial composition. Overall, the gut microbiota was not remarkably altered between VD and MD after the trial. Coprococcus was found to be increased in VD while being decreased in MD. Roseburia and Faecalibacterium were increased in MD while being decreased in VD. Importantly, changes in genera Coprococcus, Roseburia and Faecalibacterium should be subjected to intense investigation as markers for physical and mental health

CONICA design, performance and final laboratory tests

International audienc

CONICA design, performance and final laboratory tests

International audienc

Ten-micron instrument MIDI: getting ready for observations on the VLTI

International audienc

Ten-micron instrument MIDI: getting ready for observations on the VLTI

International audienc

Ten-micron instrument MIDI: getting ready for observations on the VLTI

International audienc