Wie wirken sich Geburtsverhalten und Wurfqualität auf die Aufzuchtleistung aus?

In dieser Untersuchung wurde der Effekt des Geburtsverhaltens und der Wurfqualität auf die Aufzuchtleistung in 132 Würfen von Deutsche Landrasse Sauen geprüft. Das Geburtsverhalten wurde mit einem Notensystem (sehr gut, gut, mittel, schlecht) bewertet. Die Wurfqualität wurde anhand der Vitalität und Homogenität der Ferkel zur Geburt erfasst. Von der Geburt bis zum Absetzen wurden Daten verschiedener Leistungsmerkmale gesammelt. Unterschiede zwischen Würfen mit verschiedenen Noten wurden mittels ANOVA und Tukey-Test untersucht und der Effekt des Geburtsverhaltens und der Wurfqualität auf die Aufzuchtleistung mittels eines linearen Modells (GLM) analysiert. Der Einfluss der Wurfqualität auf die Anzahl aufgezogener Ferkel, das Wurfgewicht am 35. Lebenstag und die prozentualen Saugferkelverluste war statistisch signifikant. Darüber hinaus zeigten Würfe mit hoher und niedriger Qualität einen signifikanten Unterschied bezüglich der Anzahl lebend und tot geborener Ferkel und des durchschnittlichen Geburtsgewichts der Ferkel

Supplemental Data Set 5

Categorization of Light-Regulated and Reference Gene Sets into Functional Subgroups

Supplemental Dataset 1

Computational Analysis of Transcriptome-Wide AS

Supplemental Data Set 2

Computational Analysis of Transcriptome-Wide Differential Gene Expression

Supplemental Data Set 4

Analysis of NMD Target Features and Overlap between NMD- and Light-Regulated AS

Supplemental Data Set 7

Computational Analysis of Transcriptome-Wide AS Changes in Response to Red Light Based on the Data from Shikata et al. (2014)

Data from: Alternative splicing substantially diversifies the transcriptome during early photomorphogenesis and correlates with the energy availability in Arabidopsis

Plants use light as source of energy and information to detect diurnal rhythms and seasonal changes. Sensing changing light conditions is critical to adjust plant metabolism and to initiate developmental transitions. Here we analyzed transcriptome-wide alterations in gene expression and alternative splicing (AS) of etiolated seedlings undergoing photomorphogenesis upon exposure to blue, red, or white light. Our analysis revealed massive transcriptome reprograming as reflected by differential expression of ~20% of all genes and changes in several hundred AS events. For more than 60% of all regulated AS events, light promoted the production of a presumably protein-coding variant at the expense of an mRNA with nonsense-mediated decay-triggering features. Accordingly, AS of the putative splicing factor REDUCED RED-LIGHT RESPONSES IN CRY1CRY2 BACKGROUND 1 (RRC1), previously identified as a red light signaling component, was shifted to the functional variant under light. Downstream analyses of candidate AS events pointed at a role of photoreceptor signaling only in monochromatic but not in white light. Furthermore, we demonstrated similar AS changes upon light exposure and exogenous sugar supply, with a critical involvement of kinase signaling. We propose that AS is an integration point of signaling pathways that sense and transmit information regarding the energy availability in plants

Lighting Up Nobel Prize-winning Studies with Protein Intrinsic Disorder

Intrinsically disordered proteins and regions (IDPs and IDRs) and their importance in biology are becoming increasingly recognized in biology, biochemistry, molecular biology and chemistry textbooks, as well as in current protein science and structural biology curricula. We argue that the sequence → dynamic conformational ensemble → function principle is of equal importance as the classical sequence → structure → function paradigm. To highlight this point, we describe the IDPs and/or IDRs behind the discoveries associated with 17 Nobel Prizes, 11 in Physiology or Medicine and 6 in Chemistry. The Nobel Laureates themselves did not always mention that the proteins underlying the phenomena investigated in their award-winning studies are in fact IDPs or contain IDRs. In several cases, IDP- or IDR-based molecular functions have been elucidated, while in other instances, it is recognized that the respective protein(s) contain IDRs, but the specific IDR-based molecular functions have yet to be determined. To highlight the importance of IDPs and IDRs as general principle in biology, we present here illustrative examples of IDPs/IDRs in Nobel Prize-winning mechanisms and processes