Siderose hepatica em tucanos e araçaris /

Orientador : Prof. Dr. Fabiano Montiani-FerreiraDissertaçăo (mestrado) - Universidade Federal do Paraná, Setor de Cięncias Agrárias, Programa de Pós-Graduaçăo em Ciencias Veterinárias. Defesa: Curitiba, 2008Inclui bibliografi

AVALIAÇÃO ELETROCARDIOGRÁFICA EM ANTAS (Tapirus terrestris)

O objetivo desse trabalho foi estabelecer parâmetros eletrocardiográficos para a anta (T. terrestris), servindo como referência normal para a espécie. Foram utilizados machos adultos de anta brasileira (n=11), com peso entre 160 a 220 kg e idade entre 3 a 28 anos. Os animais estavam alojados em cativeiro na Itaipu Binacional (Brasil e Paraguai). Os resultados estão expressos na forma de média ± desvio padrão. Os parâmetros obtidos em DII (derivação II) foram a frequência cardíaca (FC) = 63 ± 22 bpm, ritmo cardíaco (sinusal), eixo elétrico médio (EEM) = 70,00º ± 81,22º; duração de P = 0,05 ± 0,02 s; amplitude de P = 0,16 ± 0,07mV, duração de QRS = 0,08 ± 0,02 s; amplitude de R = 0,74 ± 0,41 mV; duração do intervalo PR = 0,19 ± 0,06 s e duração de QT = 0,40 ± 0,14 s. Na observação de dados publicados em evento cientifico, com exceção da amplitude de P (2,3 vezes maior no presente estudo), todas as outras variáveis estão próximas para a mesma espécie. Verificou-se que a FC das antas sob efeito dos anestésicos utilizados (cetamina 10%; detomidina 1%; butorfanol 1%; com adição de atropina na presença de secreção respiratória abundante) é 1,5 vezes maior que a FC média de equinos adultos não anestesiados em repouso. No intervalo PR, duração de P e amplitude de R observou-se que equinos têm valores duas a quatro vezes maiores que os das antas. A duração de QRS em antas chega a pouco menos que duas vezes menor, quando comparada com a de equinos. A FC maior pode estar relacionada a um provável maior metabolismo das antas, considerando que são animais com menor massa corporal do que os equinos. O menor intervalo PR das antas também pode ser atribuído a este fato. A menor duração de P e de QRS e da amplitude de R pode estar relacionada com o menor tamanho do coração das antas

Detection of Plasmodium sp. in capybara

In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the two capybara sequences. When compared with GenBank sequences, a 55% similarity was obtained to Toxoplasma gondii and a higher similarity (73– 77.2%) was found to ssrRNAs from Plasmodium species that infect reptile, avian, rodents, and human beings. The most similar Plasmodium sequence was from Plasmodium mexicanum that infects lizards of North America, where around 78% identity was found. This work is the first report of Plasmodium in capybaras, and due to the low similarity with other Plasmodium species, we suggest it is a new species, which, in the future could be denominated ''Plasmodium hydrochaeri''

Siderose hepatica em tucanos e araçaris /

Orientador : Prof. Dr. Fabiano Montiani-FerreiraDissertaçăo (mestrado) - Universidade Federal do Paraná, Setor de Cięncias Agrárias, Programa de Pós-Graduaçăo em Ciencias Veterinárias. Defesa: Curitiba, 2008Inclui bibliografi

Occurrence of sarcocystis falcatula in captive psittacine birds in Brazil

Thirty-eight captive psittacine birds housed in a bird park in Foz do Iguaçu, Parana, Brazil, died within a 15-month period as a result of infection with Sarcocystis falcatula. Although fatalities affected 16 species of psittacine birds, mortality was highest in Old World species, which were most susceptible to the pulmonary form of sarcocystosis. Along with the pathologic findings of this disease outbreak, a review of the pathophysiology of sarcosporidiosis is presentedFAPES

ARTs in wild felid conservation programmes in Poland and in the world

With the exception of the domestic cat, all felid species (Felidae) are currently threatened with extinction in their natural habitat. To develop effective and optimal wild cat conservation programmes with assisted reproductive technology (ART) it is necessary to combine advances from different disciplines of science, starting from the biology of the species, through research into the population and habitat, assisted reproductive technologies, establishment of gene banks, developing bioinformatic systems, and ending with biodiversity and endangered species management. In the last few years knowledge of felid reproduction has expanded considerably thanks to comparative studies utilising the domestic cat as a research model for endangered wild cats. Basic reproductive techniques utilised in both domestic cat breeding and rescuing wild felid populations that are threatened with extinction include semen collection and cryopreservation, artificial insemination, oocyte collection, in vitro maturation, in vitro fertilisation, somatic cloning, and embryo transfer. The main directions in which assisted reproductive technologies are being developed in wild cat conservation implementations and the contribution of Polish research centres in advancing these methods are presented

First molecular screening of Plasmodium species in ungulates from Southern Brazil

Abstract Objective Despite malaria epidemiology has been extensively studied in primates, few studies were conducted in ungulates. After half a century without descriptions of Plasmodium spp. in deer since its first identification, recent research has rediscovered Plasmodium on ungulates in Africa, Asia, North America and South America, including Central Brazil. Here, a captive herd was evaluated in southern Brazil using light microscopy and PCR. DNA samples were tested for fragment amplification of two Plasmodium spp. genes: mitochondrial cytochrome b and small subunit ribosomal RNA. Results All analyses were negative. However, the tests were performed on samples that were collected at a single time point, and parasitemia may fluctuate over the parasite’s life cycle. Thus, the possibility of occult infection cannot be ruled out. Despite the negative results of all of the methods applied, it cannot be categorically stated that these animals are free from Plasmodium sp. infection. Further monitoring and/or multiple sequential sampling may improve the success rate of detecting parasites. Moreover, although this survey of Plasmodium represents the first molecular study on ungulate malaria parasites from Southern Brazil, further analysis of samples from different ungulate species is important for characterizing the epidemiology of Plasmodium of these mammals in this region

Interfacial stress and container failure during freezing of bulk protein solutions can be prevented by local heating

Lisboa 2020_Project 17653_CryocubeBottles and carboys are used for frozen storage and transport of biopharmaceutical formulations under a wide range of conditions. The quality of freezing and thawing in these systems has been questioned due to the formation of heterogeneous ice structures and deformation of containers. This work shows that during freezing of bulk protein solutions, the liquid at the air-liquid interface freezes first, forming an ice crust and enclosing the liquid phase. As the enclosed liquid freezes, internal pressure rises, pushing the liquid phase through the porous ice crust towards the air interface, leading to interfacial stress and protein aggregation. The aggregation of bovine serum albumin was more intense in the foam-like ice mound that was formed at the top, where bubbles were entrapped. This was characterized experimentally with the assistance of magnetic resonance imaging (MRI). An isothermal cover is proposed to prevent the early freezing of the liquid at the air interface, attenuating substantially interfacial stress to proteins and releasing hydrostatic pressure, preserving the shape and integrity of the containers.info:eu-repo/semantics/publishedVersio