El graffiti como recurso didáctico en el ámbito educativo. El caso de Granada

For more than one decade the workshops and courses of graffiti proliferate in the primary and secondary school. From the point of view of the teacher, this type of activities is often seen as an attractive educational complement to offer to the students. The experience of having lived in Granada since 1999 has helped me to recapitulate and analyze a series of problems related to the appearance and rise of graffiti in the city and its impact on the heritage building of this one, recalling a series of events on which to extract questions about how the whole issue has been raised from the institutions involved, and from the educational field.Desde hace más de una década, en la educación primaria y secundaria proliferan los talleres y cursos de graffiti. Desde el punto de vista del docente, este tipo de actividades se suelen ver como un complemento educativo atractivo para ofrecer a los alumnos. La experiencia de haber vivido en Granada desde 1999 me ha servido para recapitular y analizar una serie de problemáticas relacionadas con la aparición y auge del graffiti en la ciudad y su impacto sobre el patrimonio inmueble de esta, rememorando una serie de acontecimientos sobre los que poder extraer interrogantes acerca de cómo se ha planteado todo este asunto desde las instituciones implicadas, y desde el ámbito educativo

La edición y difusión en el graffiti. Una aproximación a la escena granadina de los noventa

The fanzines sustained principally by the documentary photo, personified the collaboration spirit in the Graffiti of the 90s through a process of exchange of specific and unpublished information until then. We analyzed all this at a time when the distance communication system among graffiti writers was based exclusively on postal mail. Our case study focuses on a very specific context, the Graffiti scene in the city of Granada, from its gestation as a movement (1992-1993) to the arrival of the Internet (1997-1998), covering a dynamic period in which graphic and visual material was produced. In this way, we will consider the existing parallelism between the ways of doing in Graffiti and methods of editing and diffusion, analyzing the concepts of traveling, exploring and sharing informationLos fanzines, sustentados principalmente por la fotografía documental, encarnaron el espíritu de colaboración en el graffiti de la década de los noventa a través de un proceso de intercambio de información específica e inexistente hasta ese momento. Analizamos todo esto en una época en la que el sistema de comunicación a distancia entre escritores de grafiti se basaba exclusivamente en el correo postal. Nuestro caso de estudio se centra en un contexto muy concreto, la escena del graffiti en la ciudad de Granada, desde su gestación como movimiento (1992-1993) hasta la llegada de internet (1997-1998), abarcando un período dinámico de producción de material gráfico y visual. De esta forma, consideraremos el paralelismo existente entre los modos de hacer en el graffiti y las formas de edición y difusión, analizando los conceptos de viajar, explorar y compartir información.

Pràctiques de Bioquímica

El document forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de ValènciaDocument PDF amb diverses Pràctiques de Laboratori de Bioquímica General per a un Grau de Biologia, però també utilitzable en Graus afins com Bioquímica, Biomedicina i Biotecnologia. Conté les descripcions de 5 sessions pràctiques on es fan servir les tècniques bàsiques de bioquímica: valoració d’àcids febles i tampons, aïllament, purificació i caracterització de proteïnes i d’àcids nucleics, experiències amb cadena fotosintètica de cloroplasts i del metabolisme d’hidrats de carboni (fermentació i glicogen).PDF document with several Practices of Laboratory of General Biochemistry for a Degree of Biology, but also usable in Degrees related like Biochemistry, Biomedicine and Biotechnology. It contains the descriptions of 5 practical sessions where the basic techniques of biochemistry are used: assessment of weak acids and buffers, isolation, purification and characterization of proteins and nucleic acids, experiences with photosynthetic chain of chloroplasts and metabolism of carbohydrates (fermentation and glycogen)

Site-specificity of pea histone acetyltransferase B in vitro

Histone acetyltransferase B from pea embryonic axes has been purified approximately 300-fold by a combination of chromatographic procedures, including affinity chromatography on histone-agarose. The enzyme preparation has been used for the in vitro transfer of acetyl groups from [1-14C]acetyl-CoA to non-acetylated pea histone H4. Up to three acetyl groups can be introduced into the histone. The resulting mono-, di-, and triacetylated H4 isoforms were separated and sequenced to determine the acetylated sites. Only sites 5, 12, and 16 were used by histone acetyltransferase B, but no clear preference among them was observed. The absence of modification of other potentially acetylatable sites is another indication that acetylation of the different lysine residues in the N-terminal H4 tail serves as a specific signal in different nuclear processes

Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming

During the process of reprogramming to induced pluripotent stem (iPS) cells, somatic cells switch from oxidative to glycolytic metabolism, a transition associated with profound mitochondrial reorganization. Neither the importance of mitochondrial remodelling for cell reprogramming, nor the molecular mechanisms controlling this process are well understood. Here, we show that an early wave of mitochondrial fragmentation occurs upon expression of reprogramming factors. Reprogramming-induced mitochondrial fission is associated with a minor decrease in mitochondrial mass but not with mitophagy. The pro-fission factor Drp1 is phosphorylated early in reprogramming, and its knockdown and inhibition impairs both mitochondrial fragmentation and generation of iPS cell colonies. Drp1 phosphorylation depends on Erk activation in early reprogramming, which occurs, at least in part, due to downregulation of the MAP kinase phosphatase Dusp6. Taken together, our data indicate that mitochondrial fission controlled by an Erk-Drp1 axis constitutes an early and necessary step in the reprogramming process to pluripotency

c‑MYC Triggers Lipid Remodelling During Early Somatic Cell Reprogramming to Pluripotency

Metabolic rewiring and mitochondrial dynamics remodelling are hallmarks of cell reprogramming, but the roles of the reprogramming factors in these changes are not fully understood. Here we show that c-MYC induces biosynthesis of fatty acids and increases the rate of pentose phosphate pathway. Time-course profiling of fatty acids and complex lipids during cell reprogramming using lipidomics revealed a profound remodelling of the lipid content, as well as the saturation and length of their acyl chains, in a c-MYC-dependent manner. Pluripotent cells displayed abundant cardiolipins and scarce phosphatidylcholines, with a prevalence of monounsaturated acyl chains. Cells undergoing cell reprogramming showed an increase in mitochondrial membrane potential that paralleled that of mitochondrial-specific cardiolipins. We conclude that c-MYC controls the rewiring of somatic cell metabolism early in cell reprogramming by orchestrating cell proliferation, synthesis of macromolecular components and lipid remodelling, all necessary processes for a successful phenotypic transition to pluripotency

Revisión a nivel ibérico de la distribución del topillo de Cabrera o iberón, Iberomys cabrerae (Thomas, 1906)

Neste estudo é apresentado um mapa de quadrículas UTM 10x10 km para toda a área de distribuição de Iberomys cabrerae. A partir dos dados obtidos destacamos que a) as 420 quadrículas com dados se concentram em 4 grandes núcleos disjuntos (Luso-Carpetano, Montibérico, Bético y Prepirenaico), das quais o mais importante é o Luso-Carpetano e o mais pequeno, isolado e ameaçado é o Preirenáico; b) é necessário intensificar a procura de populações em áreas ainda pouco estudadas; e c) os dados disponíveis sobre a situação das populações têm uma qualidade muito desigual, diferindo igualmente entre regiões, o que não permite ter uma imagem clara do seu estado de conservação, nem desenvolver estratégias de proteção e gestão adequadas

re-habitar El Carmen : Un proyecto sobre patrimonio contemporáneo

El proyecto _re-HABITAR suponía para el propio proceder de la institución un avance más allá del reconocimiento, registro, inventario o protección patrimonial de la arquitectura del siglo XX y del Movimiento Moderno para posicionarse en la acción preventiva y conservativa de ese legado contemporáneo. Para ello, la praxis patrimonial se aferraba a un modelo: el de la vivienda social en España en la segunda mitad del siglo XX; a un caso concreto: el de la barriada de Nuestra Señora del Carmen (Recasens Méndez-Queipo de Llano, 1958); y a un requisito fundamental: analizar un objeto vivo y en uso, aún con la presencia de quienes lo vivieron y usaron desde su origen

Manual de Pràctiques Integrades de Mètodes (en Bioquímica)

El document forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de ValènciaAquest “Manual de Pràctiques Integrades de Mètodes” descriu els materials, i procediments necessaris per a la realització de experiments emprant diverses tècniques de bioquímica. Correspon a una assignatura d’un Grau en Biotecnologia, però serviria igualment per a Graus en Biologia i Bioquímica. Aquest Manual es troba organitzat en dues parts. La primera part descriu experiències sobre diferents d’aplicacions de tècniques analítiques espectroscòpiques d’un laboratori de bioquímica general. La segona part inclou experiències conduent a l’obtenció, anàlisi i caracterització d’un component biològic (una proteïna) de la mateixa forma que es fa en un laboratori d’investigació bioquímica. El manual, en seccions independents de les descripcions dels procediments, inclou les explicacions necessàries per a interpretar i processar els resultats experimentals obtinguts així com una sèrie de qüestions que serveixen com una mena d’autoavaluació.This "Manual of Integrated Practices of Methods" describes the materials, and procedures necessary for the realization of experiments using various techniques of biochemistry. It corresponds to a subject of a Degree in Biotechnology, but it would also serve for Degrees in Biology and Biochemistry. This Document is organized in two parts. The first part describes experiences on different applications of spectroscopic analytical techniques from a biochemistry laboratory. The second part includes experiences leading to the obtaining, analysis and characterization of a biological component (a protein) in the same way that it is done in a biochemical research laboratory. The manual, in separate sections of the descriptions of the procedures, includes the explanations necessary to interpret and process the experimental results obtained as well as a series of questions that serve as a kind of self-evaluation