An Inducible and Reversible Mouse Genetic Rescue System

Inducible and reversible regulation of gene expression is a powerful approach for uncovering gene function. We have established a general method to efficiently produce reversible and inducible gene knockout and rescue in mice. In this system, which we named iKO, the target gene can be turned on and off at will by treating the mice with doxycycline. This method combines two genetically modified mouse lines: a) a KO line with a tetracycline-dependent transactivator replacing the endogenous target gene, and b) a line with a tetracycline-inducible cDNA of the target gene inserted into a tightly regulated (TIGRE) genomic locus, which provides for low basal expression and high inducibility. Such a locus occurs infrequently in the genome and we have developed a method to easily introduce genes into the TIGRE site of mouse embryonic stem (ES) cells by recombinase-mediated insertion. Both KO and TIGRE lines have been engineered for high-throughput, large-scale and cost-effective production of iKO mice. As a proof of concept, we have created iKO mice in the apolipoprotein E (ApoE) gene, which allows for sensitive and quantitative phenotypic analyses. The results demonstrated reversible switching of ApoE transcription, plasma cholesterol levels, and atherosclerosis progression and regression. The iKO system shows stringent regulation and is a versatile genetic system that can easily incorporate other techniques and adapt to a wide range of applications

Моніторинг ефективності інноваційної діяльності промислового підприємства

Questions of economic evaluation of the monitoring of innovative activity in industrial enterprise are considered. A system of indexes which estimate monitoring results from different perspectives is offered: economic, financial, administrative, market, social and ecological. We present a system for the monitoring of the infrastructure of innovative activity, internal and external innovative possibilities of industrial enterprise. The proposed system of economic evaluation of the results of innovative activity monitoring is proposed for machine manufacturing enterprises in Ukraine.Розглянуто питання економічної оцінки моніторингу інноваційної активності промислового підприємства. Система показників оцінки результатів спостереження з різних точок зору пропонується: економічні, фінансові, адміністративні, ринкові, соціальні та екологічні. Ми представляємо системи для моніторингу інфраструктури інноваційної діяльності, внутрішніх і зовнішніх інноваційних можливостей промислового підприємства. Пропонована система економічної оцінки результатів моніторингу інноваційної активності пропонується для машинобудівних підприємств України

Field Flow Fractionation Handbook

Field-flow fractionation (FFF) occupies a unique niche in the field of analytical separations because it is the only technique that is capable of separating materials over the entire colloidal size range (1-1,000 nm) with high resolution. Still, FFF has not enjoyed what can be considered an explosive phase of growth, like those encountered in the development of gas and liquid chromatography. There are many theories as to why this is so, and certainly a combination of factors if responsible. FFF practitioners clearly agree that one hindrance to the widespread use of FFF as a routine tool for sizing macromolecules and colloids stems from its greatest asset. That asset is its versatility, and versatility comes with a price. Thus, even though FFF is applicable to an incredible range of materials, from macromolecules of a few thousand grams per mode or more to particles as large as 100 μm, there is no simple formula for choosing the proper subtechnique, let alone the experimental variables within a given subtechnique for a given application. One must understand the underlying mechanism of the separation and gather a certain amount of experience to apply FFF to a new separation problem with reasonable success or efficiency. The goal of this handbook is to provide a useful guide to the implementation of FFF by first-time or infrequent users, while serving as a broad reference for more experienced FFF practitioners. In addressing this goal, than handbook contains a comprehensive description of the four primary FFF subtechniques, with specific examples and applications for each. Perhaps the greatest value, of this handbook lies in the fact that the authors are experts in the respective subtechniques that they address and so are able to advise the reader, from firsthand experience, on how to avoid certain pitfalls associated with developing and implementing FFF applications.https://scholarworks.boisestate.edu/fac_books/1284/thumbnail.jp

Internalization of education: "What do our students learn?"

They discussed topical issues of improving the quality of education students of Ukrainian universities. Proposed methods of improvement of international educational programs which students have the opportunity to obtain a double diploma. Proposed methods of study, improve students' knowledge

Research of the processes of privatization and prospects of investment in the various enterprises of Ukraine: enterprises of a metallurgical complex

Deals with the prospective planning, attract foreign investment in the Metallurgical complex of Ukraine. The article focuses on the level of privatization in the industry and prospects of its development

Abbildungsnachweis

Wissensformationen sind immer auch Bildformationen. Naturwissenschaftliche Erkenntnisprozesse gehören daher einer kulturellen Praxis an, die Sehtraditionen und Wissenstraditionen gleichermaßen umfasst. Die in diesem Band versammelten Aufsätze untersuchen naturwissenschaftliche und künstlerische Visualisierungen in den Übergangszonen zwischen Kunst und Wissenschaft und verbinden auf diese Weise wissenschaftsgeschichtliche und bildwissenschaftliche Fragestellungen. Die Themen reichen von der Mikroskopie in populärwissenschaftlichen Büchern des 17. und 18. Jahrhunderts und der Debatte um die Geisterfotografie im ausgehenden 19. Jahrhundert bis hin zu Visualisierungen des Tabakmosaikvirus in der Gegenwart.Knowledge formations are always also image information. Scientific processes of knowledge therefore belong to a cultural practice that encompasses both visual and knowledge traditions. The essays collected in this volume examine scientific and artistic visualizations in the transitional zones between art and science and thus combine historical and pictorial issues. The topics range from microscopy in popular science books of the 17th and 18th centuries and the debate about ghost photography in the late 19th century to visualizations of the tobacco mosaic virus in the present