Response of MIR-1 and HSP-60 Gene Expression to Endurance Training in Heart Tissue of Rats

MicroRNAs and heat shock proteins are important factors in heart function. However, the response of these factors to exercise in the heart tissue is unclear. Here, we evaluated the impact of endurance training on the expression of MIR-1 and HSP-60 genes in heart tissue of rats. In this study, 10 male Wistar rats were randomly divided into 2 groups control and endurance training. The aerobic exercise program included running on the treadmill at speed of 25 m min-1, 5 days a week for 12 weeks. After anesthesia, we performed an autopsy to collect the heart. The expression level of MIR-1 and HSP60 were measured by Real-Time PCR. An Independent t-test was used to determine significant changes (P<0.05). After the intervention period, the expression level of the MIR-1 gene showed a significant decrease in the aerobic exercise group thank in the control group (P=0.001). However, aerobic training had no significant effect on the expression level of HSP60 in the heart (P<0.05). It seems that twelve weeks of moderate-intensity aerobic exercise can probably improve heart function

Resistance exercise in a hot environment alters serum markers in untrained males

Purpose:We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods:Ten untrained young males (26 +/- 3 years; 75.8 +/- 6 kg; 177.4 +/- 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30-60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (similar to 35 degrees C) and thermoneutral (22 degrees C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results:There were time-related changes in testosterone, HSP70, and IL-6 (P 0.05). Conclusion:RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory markers

Resistance Exercise in a Hot Environment Alters Serum Markers in Untrained Males

[EN] Purpose: We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods: Ten untrained young males (26 ± 3 years; 75.8 ± 6 kg; 177.4 ± 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30–60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (∼35◦C) and thermoneutral (22◦C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results: There were time-related changes in testosterone, HSP70, and IL-6 (P < 0.001), and cortisol and IL-15 (P < 0.05). Levels of cortisol, HSP70, and IL- 6 increased immediately for RE at 35◦C, and testosterone and IL-15 levels were decreased. Changes in serum testosterone, HSP70, cortisol, and IL-15 and IL-6 levels were reversed after 1 h. A significant time × condition interaction was observed for IL-15 and HSP70 (P 0.05). Conclusion: RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory marker

Oregano (Origanum vulgare) Consumption Reduces Oxidative Stress and Markers of Muscle Damage after Combat Readiness Tests in Soldiers

Military activities often involve high-intensity exercise that can disrupt antioxidant capacity. We investigated the effects of oregano supplementation on muscle damage, oxidative stress, and plasma antioxidant markers of soldiers performing the army combat readiness test (ACRT). Twenty-four healthy male soldiers (age: 24 ± 3 years, height: 167 ± 14 cm, mass: 66 ± 3 kg) were randomized into a placebo group (n = 12) or an oregano supplementation group (n = 12). The participants consumed a capsule containing 500 mg Origanum vulgare immediately after completing the ACRT. Blood sampling was taken before exercise, immediately after exercise, and 60 and 120 min after oregano consumption. Plasma levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (TAC), and glutathione peroxidase (GPX) were measured at the four time points. The time × group interactions were found for CK (p < 0.0001, d = 3.64), LDH (p < 0.0001, d = 1.64), MDA (p < 0.0001, d = 9.94), SOD (p < 0.0001, d = 1.88), TAC (p < 0.0001, d = 5.68) and GPX (p < 0.0001, d = 2.38). In all variables, the difference between placebo and oregano groups were significant at 60 (p < 0.0001) and 120 (p < 0.0001) minutes after ACRT test. The main effect of time was also significant for all the variables (p < 0.0001). Our results suggest that oregano supplementation has the potential to reduce muscle damage and increase oxidative capacity following ACRT. Supplementation with oregano may serve as a dietary strategy to increase preparedness and promote recovery in military recruits

Effects of Exercise Training on Bone Health Parameters in Individuals With Obesity:A Systematic Review and Meta-Analysis

International audienceBackground: Osteoporosis causes bone fragility, increasing the risk of fractures. Evidence suggests a strong correlation between obesity and fracture risk. Physical training is known to enhance bone resistance and protect from fracture; however, its osteogenic effect in the presence of obesity remains unknown.and nbsp;Objective: We sought to evaluate the influence of exercise training on bone health indices in individuals with obesity.and nbsp;Methods: This systematic literature search was conducted using common electronic databases from inception - December 2019. The following key terms (and synonyms searched for by the MeSH database) were included and combined using the operators &quot;AND, &quot; &quot;OR, &quot; &quot;NOT &quot;: [( &quot;body mass index &quot; OR obesity OR obese OR overweight OR fat mass) AND ( &quot;bone mineral density &quot; OR &quot;bone mineral content &quot; OR &quot;peak bone mass &quot; OR &quot;mechanical loading &quot; OR &quot;Osteoporosis &quot; OR &quot;bone geometry &quot; OR &quot;bone resistance &quot;) AND ( &quot;exercise training &quot; OR &quot;physical training &quot; OR &quot;strength training, &quot; OR &quot;resistance training &quot; OR &quot;aerobic training &quot; OR &quot;combined training &quot;)].and nbsp;Results: After screening, 10 studies (889 initial records) were included in the final analysis (8 different countries, 263 participants). Two studies investigated males, six females, and two, both sexes. The training duration was at least eight weeks with 2-3 sessions/week. Physical training displayed a significant trivial impact on the whole body (WB) BMD (0.13 SMD; 95% CI [0.00, 0.26], p = 0.046). Subgroup analyses indicated a significant small increase in the WB BMD (0.27 SMD; 95% CI [0.00, 0.53], p = 0.048) in the endurance training group, a non-significant trivial increase in the WB BMD (0.11 SMD; 95% CI [-0.06, 0.29], p = 0.203) in the resistance group, and a non-significant trivial increase in the WB BMD (0.03 SMD; 95% CI [-0.26, 0.32], p = 0.86) in the combined training group. In addition, a significant small decrease was found in the weight of trained subjects (-0.24 SMD; 95% CI [-0.42, -0.05], p = 0.011).and nbsp;Conclusion: Physical training has little to no effect on the WB BMD in subjects with overweight/obesity. Currently, insufficient evidence to advocate for any specific type of exercise for enhancing bone health exists for overweight/obese individuals. Investigations examining the impact of varying types of physical exercise on WB BMD of obese individuals are needed

Effect of Intensity on Changes in Cardiac Autonomic Control of Heart Rate and Arterial Stiffness After Equated Continuous Running Training Programs

[EN] Background: It is well known that exercise training has positive effects on both cardiac autonomic function and arterial stiffness (AS). However, it is not clear that which exercise training variables, intensity or volume, or both, play a crucial role in this regard. This study investigates the chronic effects of high-volume moderate-intensity training (HVMIT) and low-volume high-intensity training (LVHIT) on heart rate variability (HRV) and AS in sedentary adult men. Materials and Methods: Notably, 45 males (age: 42 ± 5.7 years) were randomly assigned to a control (n = 15), HVMIT (n = 15), or LVHIT (n = 15). The HVMIT group ran three times per week on a treadmill at 50–60% of VO2max for 45–60 min, while the LVHIT trained at 70–85% of VO2max for 25–40 min. Both training protocols were equated by caloric expenditure. HRV, pulse wave velocity (PWV), hemodynamic variables, and body composition were measured before and after 12 weeks. Results: Both protocols (i.e., HVMIT and LVHIT) significantly increased the SD of normal sinus beat intervals (SDNN) and high-frequency (HF) bands (p < 0.05) after 12 weeks. Whereas the low-frequency (LF)-HF ratio decreased significantly in both training protocols (p < 0.05); however, these changes were significantly greater in the LVHIT protocol (p < 0.05). Furthermore, the root mean square of successive RR interval differences (RMSSD) significantly increased only in the LVHIT (p < 0.05). Moreover, a significant decrease in LF and PWV was only observed following the LVHIT protocol (p < 0.05). Some measures of HRV and PWV were significantly correlated (r = 0.275–0.559; p < 0.05). Conclusion: These results show that the LVHIT protocol was more efficient for improving HRV variables and PWV than the HVMIT protocol after 12 weeks of continuous running training. Interestingly, changes in some HRV parameters were related to changes in PWV. Further studies should elaborate on the link between central and peripheral cardiovascular adaptations after continuous and intermittent training regimens differing in intensity

Adipokine modulation in obesity: evaluating the integrative impact of chlorella vulgaris supplementation and interval resistance training in obese males

Aims: To evaluate the effects of 12-week chlorella vulgaris (CV) combined with interval resistance training (IRT) on plasma levels of leptin, adiponectin and neuregulin-4 (Nrg-4) in obese men. Methods: Obese men (n = 44, BMI of 32.1 ± 1.5 kg/m2) were randomly allocated to the following groups of 11 participants per group: Control Placebo group (CP), CV supplement group (CV), Interval Resistance Training group plus Placebo (IRT + P), and Interval Resistance Training plus CV supplement group (IRT + CV). IRT was performed three times a week for 12 weeks using three sets of 10 repetitions at 60 % 1RM, and integrating an active rest interval with 15 repetitions at 20 % 1RM. Participants consumed either CV (1800 mg daily) or a placebo. Pre- and post-intervention blood samples were obtained to assess adipokines which were measured byELISA. Results: While CV or IRT separately did not alter plasma levels of leptin (p > 0.05), their combination reduced leptin levels (p = 0.007). IRT and IRT plus CV increased the plasma levels of adiponectin and Nrg-4 (p < 0.01). An intergroup comparison indicated significant elevations of adiponectin and Nrg-4 in the CV compared to the CP group (p < 0.05). Conclusion: The combination of IRT and CV modulates plasma levels of leptin, adiponectin and NRG4 more effectively than either IRT or CV separately in obese men

Effectiveness of exercise interventions in animal models of multiple sclerosis

Multiple sclerosis (MS) is associated with an impaired immune system that severely affects the spinal cord and brain, and which is marked by progressive inflammatory demyelination. Patients with MS may benefit from exercise training as a suggested course of treatment. The most commonly used animal models of studies on MS are experimental autoimmune/allergic encephalomyelitis (EAE) models. The present review intends to concisely discuss the interventions using EAE models to understand the effectiveness of exercise as treatment for MS patients and thereby provide clear perspective for future research and MS management. For the present literature review, relevant published articles on EAE animal models that reported the impacts of exercise on MS, were extracted from various databases. Existing literature support the concept that an exercise regimen can reduce the severity of some of the clinical manifestations of EAE, including neurological signs, motor function, pain, and cognitive deficits. Further results demonstrate the mechanisms of EAE suppression with information relating to the immune system, demyelination, regeneration, and exercise in EAE. The role for neurotrophic factors has also been investigated. Analyzing the existing reports, this literature review infers that EAE is a suitable animal model that can help researchers develop further understanding and treatments for MS. Besides, findings from previous animal studies supports the contention that exercise assists in ameliorating MS progression

FIFA World Cup Qatar 2022: Solutions to the Physical Fitness Challenge

In 2022, the FIFA World Cup has been scheduled to take place in Qatar in November and December, months which coincide with the in-season period of the European soccer season. This will be challenging for the staff of the participating national teams and the domestic clubs to which participating players are attached. The aim of this letter to the editor is to propose solutions on how to manage the associated challenges. Regular training and competition over the course of a season in European professional soccer is generally characterized by a pre-competition preparation period of five to six weeks, followed by two competition phases, interspersed with a winter break (Eliakim et al., 2018). Certain leagues such as the English Premier League do not typically have a winter break meaning that games are played almost continuously across the season. During World Cup years, there is usually an average of four to five weeks between the end of national domestic championships and the start of the World Cup tournament (Table 1, Figure 1) which traditionally takes place during the off-season period. However, in 2022, the FIFA World Cup has been scheduled to take place in November and December, months which coincide with the in-season period of the European soccer season (Figure 1). With the World Cup being staged during this part of the season, many national team players (notably those in the major European Leagues) will have just one week of preparation between the last match of their domestic leagues and the start of the World Cup tournament (November 20th, 2022). More precisely, the major European soccer leagues will interrupt match schedules between November 9th and 13th with differences in the number of games completed at this time of the season ranging from 14 to 17 across the various leagues (Table 2). The physical and mental demands placed on modern professional players have steadily risen over recent years due to an increase in the number of matches played during congested periods across the season (Anderson et al., 2016). Since the number of matches is not evenly distributed across the typical 40-week season, players can often compete in as many as three matches in a seven-day period. Aside from the physical and mental demands that are imposed during a match, players might experience insufficient recovery between these games; in part due to extensive travelling which can disrupt the sleep/wake cycle (Lastella et al., 2019). Indeed, poor quality of sleep and the stress induced by a match can negatively affect physical fitness and may even increase the risk of sustaining injuries and/or infections (Clemente et al., 2021) in the period leading up to the World Cup. National teams are composed of players from different leagues who have varying levels of exposure to match-play (e.g., starters, non-starters) in terms of the average weekly volume of soccer matches at their clubs (“Rapports - Observatoire du football CIES”). Moreover, both starters and non-starters are exposed to different external match and training loads (Anderson et al., 2016). External loads have previously been defined as the overall volume of activity that a player performs during both training sessions and matches (Ravé et al., 2020). There is evidence that this metric correlates with a player’s physical fitness status (Clemente et al., 2019) and their injury risk (Malone et al., 2017). Accordingly, it will be challenging for national teams to manage the fitness of players such that they are physically ready to play at the World Cup tournament. This is especially applicable to individuals who play in the major European leagues and we note a significant contrast between European match schedules and those on other continents. For example, in Major League Soccer (MLS) in North America, match schedules will be interrupted from November 5th, 15 days before the World Cup tournament begins. Similarly, in the Japanese J-League in Asia, Saudi Pro League and Qatar Star League, matches will be interrupted one month before the World Cup tournament begins, leaving more time for players on these continents to prepare. It is also important to note that the French, Spanish and English domestic championships will resume their match schedules on December 27th which is just ten days after the end of the World Cup (Figure 1). Clubs will clearly want their players to return uninjured and with sufficient fitness levels to resume domestic competition but these goals could be compromised by the aforementioned scheduling of the World Cup tournament.</jats:p

Single and Concurrent Effects of Endurance and Resistance Training on Plasma Visfatin, Insulin, Glucose and Insulin Resistance of Non-Athlete Men with Obesity

The purpose of the present study was to investigate the effect of endurance (ET), resistance (RT) and concurrent training (CT) on plasma levels of visfatin, insulin, glucose and insulin resistance of non-athlete men with Obesity. It was a semi-experimental study. Thirty six men [age: 21.48 (0.25), and BF%: 27.39 (0.52)], voluntarily participated in this study after public announcement in university. Main inclusion and exclusion criteria was healthy (no physical illness and inability), obesity [based on WHO's definition body fat percentage (BF%) of over 25] and non-athlete (without regular training during week). They were randomly divided into three groups (n=12) for ET, RT and CT. For 8 weeks (3sessions/week), the candidates participated in ET (25-40 min at 65-85% of maximum heart rate), RT (5exercises, 6sets, intensity: 50-80% of one repetition maximum, volumes: 5, 8 and 12repetitions) and CT (one or a half-term ET and then RT with 3 sets). Blood samples were taken 48 h before the first training session and 48 h after the last training session. The ANCOVA was used for the comparison of the effect of three types of training. The BF% in ET was significantly less than that in RT (p<0.01), and in CT, it was less than that for both ET and RT (p<0.01). Plasma visfatin only, in CT was significantly less than that in RT (p<0.01). Plasma insulin levels in CT were significantly higher than that in ET and RT (p<0.01). Plasma glucose levels in CT were less than that in ET and RT, significantly (p<0.01). Insulin resistance only in CT was less than that in ET significantly (p<0.01). In general, the present study showed that maybe, CT have more effect on the body composition, glucose metabolism and insulin resistance adjustment, which can be effective in preventing obesity and adjusting adipocytokines such as visfatin