ESTABLISHMENT AND REORGANIZATION OF DERIVED ARBITRARY RELATIONS FOLLOWING IDENTITY MATCHING-TO-SAMPLE TRAINING WITH SPECIFIC CONSEQUENCES

No Experimento 1, seis participantes foram treinados em um procedimento MTS de identidade (IDMTS) para estabelecer relações estímulo-estímulo A1A1, A2A2, A3A3; B1B1, B2B2, B3B3; e C1C1, C2C2, C3C3. Respostas corretas resultavam na apresentação de consequências específicas, Sr1, Sr2 e Sr3. Uma resposta de consumação às consequências específicas foi requerida para que os participantes pudessem coletar os pontos nas tentativas de treino. O procedimento MTS arbitrário foi utilizado para documentar a formação de três classes de equivalência A1B1C1Sr1, A2B2C2Sr2 e A3B3C3Sr3. Em uma fase subsequente, o IDMTS com consequências específicas foi empregado para treinar as relações D1D1, D2D2 e D3D3. Os estímulos D eram fotografias de faces humanas expressando alegria (D1), tristeza (D2) e neutralidade (D3). Por último, avaliações por meio do diferencial semântico atestaram a ocorrência da transferência das funções (i.e., “significados”) dos estímulos D para os estímulos C e para Sr1 e Sr3. Estes resultados sugeriram que as classes ABCSr se expandiram para incluir os estímulos significativos D (i.e., A1B1C1D1Sr1, A2B2C2D2Sr2 e A3B3C3D3Sr3). No Experimento 2 verificou-se que, para cinco participantes, o procedimento de reversão das consequências específicas Sr1 e Sr2 em relação aos estímulos C1, C2 e D1 e D2 deu origem a duas classes de equivalência reorganizadas (A1B1C2D2Sr2 e A2B2C1D1Sr1). Paralelamente, os dados do diferencial semântico documentaram a reversão dos significados atribuídos previamente aos estímulos D1 e D2. Estes achados sugerem que o procedimento de reversão reorganizou as classes de estímulos bem como seus significados. Porém, resultados análogos não foram reportados para as consequências específicas.Palavras-chave: Matching-to-sample de identidade, consequências específicas, classes de equivalência, reorganização de classes de equivalência, transferência de função, universitáriosIn Experiment 1, six participants were given identity matching to sample (IDMTS) training that established the stimulus- stimulus relations A1A1, A2A2, A3A3; B1B1, B2B2, B3B3; and C1C1, C2C2, C3C3. Correct responses resulted in presentations of class-specific consequences, Sr1, Sr2, and Sr3. Consummatory responses to the class-specific consequences were required to collect points on training trials. Subsequent tests used arbitrary matching to sample to confirm the formation of three equivalence classes, A1B1C1Sr1, A2B2C2Sr2, and A3B3C3Sr3. Next, IDMTS, with the specific consequences, was used to train D1D1, D2D2 and D3D3 relations. These D stimuli were pictures of human faces portraying happiness (D1), sadness (D2), and neutrality (D3). Finally, tests used semantic differential ratings to document transfer of functions (i.e., “meanings”) of the D stimuli to the C stimuli and to the Sr1 and Sr3 stimuli, in accord with class membership. These results suggest expansion of the ABCSr classes to include the meaningful D stimuli (i.e., A1B1C1D1Sr1, A2B2C2D2Sr2, and A3B3C3D3Sr3). Experiment 2 verified that, for five participants, reversals of the class-specific consequences Sr1 and Sr2 with respect to stimuli C1, and C2, and D1 and D2, gave rise to the two reorganized equivalence classes A1B1C2D2Sr2 and A2B2C1D1Sr1. Semantic differential ratings then documented reversals of the meanings given to stimuli C1 and C2. Also, semantic differential ratings documented the reversals of the meanings of C stimuli in accordance with their reorganized class-memberships with D stimuli. However, analogous reversals of function transfer were not observed for the class-specific consequences. Key words: Identity matching-to-sample, class-specific consequences, equivalence classes, reorganization of equivalence classes, transfer of functions, undergraduates

Heat waves in the capitals of southern Brazil and Montevideo - Uruguay

Múltiplas partes do globo, possivelmente, passarão a ter dias e noites mais quentes e, com a elevação das temperaturas globais, há tendências de acréscimo do risco de eventos atmosféricos extremos, tais como as ondas de calor. O objetivo principal desse estudo foi verificar as características gerais das ondas de calor nas três capitais da região Sul do Brasil (Curitiba, Florianópolis e Porto Alegre) e também em Montevidéu, capital do Uruguai. Esta análise baseou-se nos parâmetros: frequência, intensidade, duração e suas respectivas tendências. As ondas de calor foram identificadas em uma série de 30 anos de dados diários de temperatura média do ar. As análises de tendência foram averiguadas por meio do teste de Mann-Kendall a um nível de significância de α = 5%. Os resultados mostraram que todos os parâmetros nas quatro cidades apresentam tendências estatisticamente significativas e, com exceção da duração em Montevidéu, todas as demais são positivas. Para além do âmbito das tendências, analisando os parâmetros, comparativamente, conclui-se que Porto Alegre apresenta maior destaque nas médias. Além disto, afirma-se que o inverno é a estação com maior frequência de ondas de calor para todas as cidades.Multiple parts of the globe are likely to have warmer days and nights, and with rising global temperatures, there is a tendency to increase the risk of extreme weather events, such as heat waves. The main objective of this study was to verify the general characteristics of heat waves in the three capitals of southern Brazil (Curitiba, Florianópolis and Porto Alegre) and also in Montevideo, capital of Uruguay. This analysis was based on the parameters: frequency, intensity, duration and their respective trends. Heat waves were identified in a series of 30 years of daily average air temperature data. Trend analyzes were performed using the Mann-Kendall test at a significance level of α = 5%. The results showed that all the parameters in the four cities present statistically significant trends and, except for the duration in Montevideo, all the others are positive. In addition to the scope of the trends, analyzing the parameters, comparatively, it is concluded that Porto Alegre presents greater prominence in the averages. In addition, it is claimed that winter is the season with the highest frequency of heat waves for all cities

Design and validation of an innovative 3D printer containing a co-rotating twin screw extrusion unit

This paper presents the design and validation of an innovative 3D printer containing a co-rotating twin screw extrusion unit (Co-TSE). Single screw print heads were developed in the mid-2000s as an alternative to filament-based 3D printers, but they have limited process flexibility and mixing capacity. The new design accepts material in powder or micro-pellet form, and its dispersive and distributive mixing capacity can be fine tuned by setting output and screw rotation speed independently. The design combines a miniaturized modular Co-TSE operated under starve-fed conditions with a benchtop Cartesian platform. Numerical calculations were performed to ascertain whether the appropriate thermomechanical environment for polymer processing could be created by the proposed design. A prototype was built and extrusion tests were performed under different operating conditions, using polypropylene and a 90/10 wt% polypropylene/polystyrene blend. Two screw configurations were used, with and without kneading discs, to assess the response of the extrusion unit in terms of flow characteristics and mixing performance. The restriction to flow created by the mixing elements determines the starting melt position, and the average residence times, while their shearing and extensional action enhances homogenization effectiveness. The screw configuration and rotation speed do not affect the output, which depends only on the feed rate. Preliminary deposition tests were conducted to determine the feasible printing parameters. A standard tensile test specimen, a square scaffold and a multicolored rectangular box were successfully printed, validating the innovative design. The mechanical properties of printed test specimens were within the expected values.This work was supported by the National Council for Scientific and Technological Development (CNPq), grants 2016-4/442109 and 142348/2018-0, and by the Coordination for the Improvement of Higher Education Personnel (CAPES), finance code 001

Tipificação de amostras aviárias patogênicas de Escherichia coli pela REP-PCR

In the present study the repetitive extragenic palindromic (REP) polymerase chain reaction (PCR) technique was used to establish the clonal variability of 49 avian Escherichia coli (APEC) strains isolated from different outbreak cases of septicemia (n=24), swollen head syndrome (n=14) and omphalitis (n=11). Thirty commensal strains isolated from poultry with no signs of these illnesses were used as control strains. The purified DNA of these strains produced electrophoretic profiles ranging from 0 to 15 bands with molecular sizes varying from 100 bp to 6.1 kb, allowing the grouping of the 79 strains into a dendrogram containing 49 REP-types. Although REP-PCR showed good discriminating power it was not able to group the strains either into specific pathogenic classes or to differentiate between pathogenic and non-pathogenic strains. On the contrary, we recently demonstrated that other techniques such as ERIC-PCR and isoenzyme profiles are appropriate to discriminate between commensal and APEC strains and also to group these strains into specific pathogenic classes. In conclusion, REP-PCR seems to be a technique neither efficient nor universal for APEC strains discrimination. However, the population clonal structure obtained with the use of REP-PCR must not be ignored particularly if one takes into account that the APEC pathogenic mechanisms are not completely understood yet.A técnica de REP (Repetitive extragenic palindrome)-PCR foi utilizada para avaliar a variabilidade genética de 49 amostras de Escherichia coli patogênicas para aves (APEC), isoladas de aves de corte (frangos) em diferentes surtos de septicemia (n=24), síndrome da cabeça inchada (n=14) e onfalite (n=11). Trinta amostras comensais, isoladas de frangos sem sinais de doença, foram utilizadas como controle. A análise do perfil eletroforético obtido por reação de REP-PCR utilizando DNA purificado das amostras evidenciou a amplificação de 0 a 15 bandas de DNA com pesos moleculares variando entre 100 pb e 6.1 Kb. A análise deste padrão permitiu a construção de um dendrograma demonstrando o agrupamento das 79 amostras em 49 perfis distintos. Embora a técnica de REP-PCR tenha apresentado grande poder discriminatório, as amostras patogênicas e não patogênicas não foram discriminadas entre si assim como não foi observado o agrupamento de amostras causadoras do mesmo tipo de doença. Por outro lado, demonstramos recentemente que outras técnicas tais como ERIC-PCR e a análise de isoenzimas foram eficientes quando utilizadas para esta mesma finalidade. Concluindo, REP-PCR parece não ser uma técnica eficiente e universal para discriminar entre amostras APEC. Porém, a estrutura clonal populacional obtida com o uso de REP-PCR não deve ser desprezada, particularmente se considerarmos que os mecanismos de patogenicidade de APEC ainda não são completamente conhecidos.6973Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

In vitro maturation impacts cumulus–oocyte complex metabolism and stress in cattle

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOThe influence of in vitro maturation (IVM) in oocytes is still not totally understood. The aim of this study was to determine the influence of IVM on the metabolism and homeostasis of bovine cumulus-oocyte complexes. In the present study, we demonstrated1546881893FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO2014/21034-32014/03281-32014/22887-02013/08135-22012/50533-2306978/2014-8The authors would like to thank the staff and students at the LMMD, Marcos Chiaratti, Gustavo Duarte, Marcel Nakashima, Hélio Alves Martins Júnior, José Luis Paz Jara, Patricia Kubo Fontes and Augusto de Castro Netto for their assistance with the sample

Alterações na composição corporal de meninos submetidos a um programa de treinamento de força

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Swimming exercise modifies oxidative stress in skeletal and cardiac muscles of diabetic rats

Introduction: Oxidative stress is a key factor leading to the deterioration of diabetes. Oxidative stress exacerbates diabetes and induction of the activity of the antioxidant system may be required to prevent this effect. Objetive: The aim of the present study was to evaluate the redox state in the skeletal and cardiac muscles in a diabetes rat model subjected to swimming exercise for 4 weeks. Methods: Wistar rats were divided into four groups: untrained control (C), trained control (T), untrained alloxan-induced diabetes (D), and trained alloxan-induced diabetes (TD). The redox state of the skeletal and cardiac muscles was assessed by analyzing TBARS, -SH groups, H2O2 production, and SOD and catalase activity. The total number of cardiomyocytes and the total area of collagen fibers in the cardiac muscle were measured by histomorphometry. Results: In the Soleus muscles, the TD group showed increased H2O2 levels and catalase activity compared to the T group, and SOD activity compared to the D group. Regarding the red gastrocnemius, the TD group presented higher SOD and lower catalase activities than the D group. Regarding the cardiac muscle, the TD group presented lower TBARS and higher levels of -SH groups and catalase activity than the D group. Swimming exercise decreased hyperglycemia and reduced pathology, as evidenced by the reduced number of cardiomyocytes and the area of collagen fibers. Conclusion: Swimming exercise in diabetic rats controlled hyperglycemia and oxidative damage, and the reduced fibrosis in the cardiac muscle of diabetic rats

Ticks collected on birds in the state of São Paulo, Brazil

Abstract The present study reports a collection of Amblyomma spp. ticks in birds from several areas of the state of São Paulo, Brazil. A total of 568 tick specimens (404 larvae, 164 nymphs) were collected from 261 bird specimens. From these ticks, 204 (36%) specimens (94 larvae, 110 nymphs) were reared to the adult stage, being identiWed as Amblyomma longirostre (94 larvae, 90 nymphs), Amblyomma calcaratum (13 nymphs), Amblyomma nodosum (2 nymphs), and Amblyomma cajennense (5 nymphs). Additionally, 39 larvae reared to the nymphal stage and 8 nymphs that died before reaching the adult stage were identiWed as A. longirostre according to peculiar characters inherent to the nymphal stage of this species: scutum elongate, and hypostome pointed. The remaining 271 larvae and 46 nymphs were identiWed as Amblyomma sp. Ticks were collected from 51 species of birds distributed in 22 bird families and 6 orders. The order Passeriformes constituted the vast majority of the records, comprising 253 (97%) out of the 261 infested birds. Subadults of A. longirostre were identiWed from 35 species of Passeriformes, comprising 11 families (Cardinalidae, Dendrocolaptidae, Fringillidae, Furnariidae, Parulidae, Pipridae, Thamnophilidae, Thraupidae, Turdidae, Tyrannidae, and Vireonidae), and from 1 specie