Ultrastructural changes in renal parenchyma with renal cell carcinoma

Pasichnyk S., Stakhovsky O., Gozhenko A., Pasichnyk M., Voronina O. Ultrastructural changes in renal parenchyma with renal cell carcinoma. Journal of Education, Health and Sport. 2018;8(6):446-451. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1495561 http://ojs.ukw.edu.pl/index.php/johs/article/view/6319 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part b item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author(s) 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.06.2018. Revised: 12.06.2018. Accepted: 29.06.2018. Ultrastructural changes in renal parenchyma with renal cell carcinoma S. Pasichnyk1, O. Stakhovsky2, A. Gozhenko3, M. Pasichnyk1, O. Voronina4 1Lviv National Medical University named after Danylo Halytsky, Lviv, Ukraine Department of urology FPGE 2National Cancer Institute, Kiyv 3Ukrainian Research Institute of Transport Medicine of Ministry of Health of Ukraine, Odessa 4NSC Institute of Biology and Medicine, Kiyv Abstract In recent years, there has been a significant increase in incidence of the renal cell carcinoma (RCC). In the structure of cancer, this pathology is on 10th place. The surgical treatment is the most radical and most effective method of management of this disease. Currently renal resection has become a desirable alternative to radical nephrectomy for tumors of the T1 stage [6, 7, 8, 9]. Nephron-sparing interventions, compared to radical nephrectomy, showed an equivalent cancer outcome in tumors < 4 cm in diameter [1, 10]. However, there is an opinion [2] that in the intact part of the organ due to an increased functional load various pathomorphological changes may occur [3, 4 ,7]. Although, it is believed that in this cohort of patients there are no pathomorphological changes and only in rare cases, distant functional changes can be observed [5, 2]. Key words: renal cell carcinoma, nephron, nephrectomy, nephron-sparing surger

Endothelial dysfunction in patients after COVID-19

Relevance: Over the past two years, the COVID-19 infection has gained unprecedented relevance at the global level. All countries are spending significant resources in the fight against COVID-19 [1 2]. The purpose of the work is to clarify the issue of the state of the kidneys in the distant post-covid period (after 2-3 months), we conducted a study of the kidneys simultaneously with the desquamation of the endothelium, as one of the probable mechanisms of the nephrotic effect of the COVID-19 virus. Materials and methods: 29 people participated in the study. Patients with post-covid syndrome (n=10) were treated on an outpatient basis at the "KNP "Center of primary medical and sanitary (medical and sanitary) care No. 3, Odesa" of the Odesa City Council" in the period from 2020 to 2021. The control group consisted of patients without cardiovascular and endocrine disorders, comparable in terms of gender and age, who were examined in 2018 at the Odesa Regional Clinical Medical Center of the Odesa Regional Council. Endothelial dysfunction "ED" was determined by the intensity of desquamation of the endothelium according to the method of J. Hladovec as modified by M.M. Petryschev. and other. (2001).           Statistical analysis was performed using Status software [https://status-please.herokuapp.com/]. Mean values ​​(M) and its standard deviation (SD) were used to describe quantitative data, and percentages were used for qualitative data. For comparison, data were presented as M and standard error of the mean (SEM). Analysis of normality was performed using the Shapiro-Wilk test. Levene's test was used to assess homogeneity of variances. Differences between groups were determined using Student's t-test and one-way analysis of variance (ANOVA). The relationship and presence of correlation between the studied parameters was determined using the Pearson correlation coefficient (r). The correlation coefficient in the range of 0.5≤|r|&lt;1 indicated a strong correlation relationship, 0.3≤|r|&lt;0.5 – a relationship of medium strength, 0&lt;|r|&lt;0.3 – on a weak correlation. Statistical significance was established only at the level of p &lt; 0.05. Results: 17 men and 12 women aged from 32 to 80 years who suffered from a disease of moderate severity participated in the study. The average age of the group of patients with post-covid syndrome (n=9) is 55.9±14.47 years, the average age of the control group (n=20) is 55.95±14.47 years. There were no complaints from the urinary system in the examined patients. According to blood serum tests, it was established that the creatinine level among patients with post-covid syndrome was on average 113±11.91 μmol/l, the urea level was 8.11±1.2 mmol/l, the GFR level was 55.06±12, 19 ml/min/1.73. m2. In the control group, the numbers were as follows: creatinine level was 73.2±8.5 μmol/l, mean urea level was 4.61 mmol/l, GFR was 94.78±13.76 ml/min/1.73 m2. Conclusions: 2-3 months after the transfer of COVID-19, the examined patients show signs of chronic kidney disease. After a disease with COVID-19, an increase in CEC is detected, which is a manifestation of endothelial dysfunction

Justification of the renoprotective action of the mixture of sodium chloride and sodium bicarbonate solutions in phenylhydrazine intoxication

The authors conducted a study on 35 white Wistar outbred rats to investigate the possibility of correcting acute kidney damage induced by the administration of phenylhydrazine at a dose of 100 mg/kg by introducing a mixture of sodium chloride and sodium bicarbonate solutions into the body. The research results identified changes in the kidneys upon phenylhydrazine administration, including the loss of some capillary glomeruli, eosinophilic deposits in Bowman's spaces and tubular lumens, and lymphoid infiltration in the interstitium. Rats receiving a mixture of sodium chloride and sodium bicarbonate solutions in their drinking water showed positive changes in their kidneys: no loss of capillary glomeruli was observed, and eosinophilic deposits were absent in most tubules. Lymphocyte aggregation was only observed around some renal vessels. The authors suggest that the intake of additional sodium and bicarbonate into the body, along with alkalinization of the primary urine, promotes the excretion of hemolysis products caused by phenylhydrazine, which contributes to renoprotection and preservation of renal parenchyma

Theoretical foundations and innovative model of occupational health

Gozhenko A. I., Badyuk N. S., Lebedeva T. L., Goncharenko O. O., Zukow W. Theoretical foundations and innovative model of occupational health. Journal of Education, Health and Sport. 2016;6(11):383-389. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.168237http://ojs.ukw.edu.pl/index.php/johs/article/view/4011   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 01.11.2016. Revised 12.11.2016. Accepted: 23.11.2016.   Theoretical foundations and innovative model of occupational health Gozhenko A. I.1, Badyuk N. S.1, Lebedeva T. L.1, Goncharenko O. O.1, Zukow W.2 1Ukrainian Research Institute of Transport Medicine, Odessa, Ukraine2Kazimierz Wielki University, Bydgoszcz, Poland     AbstractHealth is a basic human value. One of the main criteria for health is still a rate of a, which in civilization’s countries is constantly decreasing, which causes the lengthening of life.However, along with this significantly changes the structure of morbidity: reduction of infectious diseases accompanied by increased infectious. All of these diseases can be divided into professional and vocational-conditioned.   Key words: health, disease, occupational diseases, professional diseases due to

Cardio-renal mechanisms of adaptation in normal and with heart failure

The pathogenesis of chronic heart failure (CHF) is a complex multifactorial process, which is a close combination of manifestations of the effects on the cardiovascular system of the etiological factor (s) and the mobilization of a whole complex of compensatory mechanisms [30]. According to the first conceptual models (cardiac (1950s) and cardiorenal (1960s)) pathogenesis, the main role in the formation of CHF belonged to a decrease in cardiac contractility (systolic dysfunction), as well as electrolyte retention and body water. [4, 14, 39]. The aim of the study was to study the role of the renin-angiotensin-aldosterone system and the natriuretic hormone in adaptive changes in water-salt homeostasis with an increase in venous blood return to the heart in healthy and in patients with stable ischemic heart disease with chronic heart failure stage I (stage I). The results of the study concluded: - in healthy individuals, an increase in venous return of blood to the heart (preload) in anti-orthostasis on the background of 0.5% water load is accompanied by adaptive changes in cardiohemodynamics, volemic homeostasis and neurohumoral regulation of water-salt metabolism, manifested by an increased influence of the parasympathetic department ANS, a decrease in heart rate, an increase in ASI while maintaining the IOC at the same level, an increase in diuresis, a decrease in the activity of the RAAS, an increase in the content of α-PNOG; - in patients with coronary artery disease with HNK I st. with an increase in preload at the 30th minute of anti-orthostasis, changes in cardio hemodynamics (decrease in IOC) and cardiac rhythmograms were revealed, indicating a depletion of myocardial reserve capacity and vegetative regulation of heart rhythm. The lack of heart rate dynamics and cardiac rhythmograms in anti-orthostasis reflects an increase in the activity of the sympathetic section of the autonomic nervous system in patients with coronary artery disease with the initial stage of HNK; - kidney reaction to anti-orthostasis and 0.5% water load in patients with coronary artery disease with HNK, I st. expressed in increased diuresis in the absence of changes in the activity of the RAAS, which indicates the predominance of antinatriuretic systems over natriuretic; - the earliest indicator of the development of heart failure in patients with coronary artery disease is sodium retention, aimed at increasing the BCC, venous return of blood to the heart and stimulation of the heterometric mechanism of Frank-Starling

Дослідження морфофункціональних особливостей розвитку експериментальних металонефропатій = The study of morphofunctional features of the development of experimental metallonephropathy

Gozhenko A. I., Lugovskoy S. P., Samokhina N. A. Дослідження морфофункціональних особливостей розвитку експериментальних металонефропатій = The study of morphofunctional features of the development of experimental metallonephropathy. Journal of Education, Health and Sport. 2016;6(4):287-296. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.50201http://ojs.ukw.edu.pl/index.php/johs/article/view/3473 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 20.03.2016. Revised 17.04.2016. Accepted: 17.04.2016. ДОСЛІДЖЕННЯ МОРФОФУНКЦІОНАЛЬНИХ ОСОБЛИВОСТЕЙ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНИХ МЕТАЛОНЕФРОПАТІЙ  А. І. Гоженко, С. П. Луговський, Н. А. Самохіна  Державне підприємство «Український науково-дослідний інститутмедицини транспорту МОЗ України», м. Одеса, Украина РезюмеПроведені експериментальні дослідження щодо тривалого впливу солей кадмію, ртуті, свинцю в дозі 1/50 від DL50 на нирки показали, що важливим аспектом їх дії є зміна показників азотистого обміну, яка проявлялась підвищеним вмістом креатиніну, сечовини та сечової кислоти в сироватці крові, а також високим вмістом білку в сечі. Характерною ознакою ураження нирок є зниження швидкості клубочкової фільтрації, яка визначалась при дії всіх трьох досліджуваних нефротоксикантів: при дії Cd – зниження на 23,6 %, при дії Pb – на 22,1 %, при дії Hg – 28,9 %. Морфологічні дослідження дозволили встановити нові факти, щодо морфологічної характеристики токсичної дії неорганічних сполук Cd, Hg, Pb, які полягають у здатності металів викликати цитопатичну дію на окремі клітини структурних елементів нефрону. Провідним механізмом розвитку експериментальних металонефропатій є ураження епітелію проксимальних канальців нирок, яке зумовлено вибірковим порушенням клітинних мембран, мітохондрій і лізосом епітелію проксимальних канальців нефрону.Ключові слова: важкі метали, нирки, металонефропатії, креатинін, сечовина, білок, швидкість клубочкової фільтрації, гістологічне дослідження нирок, канальцевий апарат, епітеліоцити. THE STUDY OF MORPHOFUNCTIONAL FEATURES OF THE DEVELOPMENT OF EXPERIMENTAL METALLONEPHROPATHY A. I. Gozhenko, S. P. Lugovskoy, N. A. Samokhina  Ukrainian Scientific and Research Institute of Transport Medicine, Odessa SummaryEexperimental studies of prolonged exposure of cadmium salts, mercury, lead in the dose of from 1/50 DL50 on the kidney have shown that an important aspect of their actions is the change of indicators of nitrogen metabolism, which was manifested by increased creatinine, urea and uric acid in serum and high urine protein excretion. A characteristic feature of renal disease is the decline in glomerular filtration rate, which was defined when all three of the studied nephrotoxicants: under the Cd decrease by 23,6 %, under the action of Pb - 22,1 %, under the action of Hg – 28,9 %. Morphological studies revealed new facts concerning the morphological characteristics of toxic action of inorganic compounds of Cd, Hg, Pb, which lies in the ability of metals to cause cytopathic effect on individual cells of the structural elements of the nephron. The main mechanism for the development of experimental metallonephropathy is the damage to the epithelium of the proximal tubules of the kidneys, due to a selective disturbance of cellular membranes, mitochondria and lysosomes in the epithelium of the proximal tubules of the nephron.Key words: heavy metals, kidneys, metallonephropathy, creatinine, urea, protein, glomerular filtration rate, and histological examination of the kidneys, tubular apparatus, the epithelial cells. ИССЛЕДОВАНИЕ МОРФОФУНКЦИОНАЛЬНЫХ ОСОБЕННОСТЕЙ РАЗВИТИЯ ЭКСПЕРИМЕНТАЛЬНЫХ МЕТАЛЛОНЕФРОПАТИЙ  А. И. Гоженко, С. П. Луговской, Н. А. Самохина Украинский НДИ медицины транспорта, г.Одесса РезюмеПроведенные экспериментальные исследования длительного воздействия солей кадмия, ртути, свинца в дозе 1/50 DL50 на почки показали, что важным аспектом их действия является изменение показателей азотистого обмена, которое проявлялось повышенным содержанием креатинина, мочевины и мочевой кислоты в сыворотке крови, а также высоким содержанием белка в моче. Характерным признаком поражения почек является снижение скорости клубочковой фильтрации, которая определялась при действии всех трех исследуемых нефротоксикантов: при действии Cd – снижение на 23,6 %, при действии Pb – на 22,1 %, при действии Hg – 28,9 %. Морфологические исследования позволили установить новые факты, относительно морфологической характеристики токсического действия неорганических соединений Cd, Hg, Pb, которые заключаются в способности металлов вызывать цитопатическое действие на отдельные клетки структурных элементов нефрона. Ведущим механизмом развития экспериментальных металонефропатий является поражение эпителия проксимальных канальцев почек, которое обусловлено выборочным нарушением клеточных мембран, митохондрий и лизосом эпителия проксимальных канальцев нефрона.Ключевые слова: тяжелые металлы, почки, металлонефропатии, креатинин, мочевина, белок, скорость клубочковой фильтрации, гистологическое исследование почек, канальцевый аппарат, эпителиоциты

Relationships between geomagnetic Ар-indeх and EEG parameters in patients with dysfunction of the neuroendocrine-immune complex

Background. Recently, on the example of two cohort of patients, we found that disturbances of the geomagnetic field cause a significant immediate modulating effect on the level of immune parameters in the blood. The data available in the literature give grounds for assumptions about the direct effect of disturbances of the geomagnetic field on immunocytes, and indirectly, through immunotropic neurotransmitters and hormones. Our hypothesis is as follows. Disturbances of the geomagnetic field are perceived by acupuncture points (APs). The information obtained is transmitted to neurons and endocrinocytes, the mediators of which, in turn, affect immunocytes. The purpose of this study is to test this hypothesis. Methods. The object of observation were 21 men (24-63 y) and 20 women (30-72 y) with neuroendocrine-immune complex dysfunction. Each patient was tested twice with an interval of 4 days. We recorded the ongoing electroencephalogram (EEG). Retrospectively we recorded the geomagnetic Ap-Index on the day of testing and during the previous 7 days, using resource https://www.spaceweatherlive.com/. Results. The canonical correlation between Ap-indices for 7 days before and on the day of testing, and EEG parameters is 0,886; immunity parameters is 0,921. In turn, the immune parameters are closely related to the EEG parameters (R=0,944). Conclusion. Disturbances of the geomagnetic field (Ap-index) causes a significant immediate modulating effect on the parameters of immunity as well as EEG, apparently through acupuncture points as polymodal receptors of the ecoceptive sensitivity system

Morpho-functional basis of endothelial dysfunction in diabetes mellitus

The diabetes mellitus (DM) inevitably progresses and leads to complications, among which the main place is occupied by micro- and macroangiopathies. The presence of endothelial damage in DM can be established even before macroscopically significant damage to the vessel. At the same time, there is no summary ED characteristic for diabetes. The aim of the study is to make a comprehensive evaluation of ED in DM -1 and DM-2 types.Materials and methods of research. 60 persons, including 53 DM type 1 and type 2, with a severe course (state of decompensation) participated in the present study. We used the method of estimating ED by the number of circulating desquamation endothelial cells (CECs) at the stages of decomposition with simultaneous determination of NO2- and NO3- metabolites of nitric oxide.Results and discussion. In patients with diabetes, the level of CECs increased in 3-5 times and ranged from 1800 to 11,200 cells / ml. The average amount of CECs in patients with diabetes was 3358.5 ± 366.3 cells / ml.Conclusions: Endothelium is involved in the pathological process at DM. This is evidenced by a significant increase in CECs in the blood plasma. The use of this method allows to detect ED before clinically considerable vascular impairment and reflects the severity of the course and duration of DM

Information sphere: prospects of development and social risks

In article the increasing relevance and the information importance in spheres of the modern habitat of the person from a position of a scientific discourse, public attention and dialogue of the international community is considere

Особенности осморегулирующей функции почек у больных с сахарным диабетом 1 типа = Features of the renal osmoregulatory function in patients with type 1 diabetes

Kuznetsova E. S., Kuznetsova E. N., Shuhtin V. V., Gozhenko A. I. Особенности осморегулирующей функции почек у больных с сахарным диабетом 1 типа = Features of the renal osmoregulatory function in patients with type 1 diabetes. Journal of Education, Health and Sport. 2015;5(8):64-72. ISSN 2391-8306. DOI 10.5281/zenodo.23452http://dx.doi.org/10.5281/zenodo.23452https://pbn.nauka.gov.pl/works/603458POL-index https://pbn.nauka.gov.pl/polindex/browse/article/article-723295be-1b7a-4fb7-977a-b926e021635bhttp://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%288%29%3A64-72Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja.Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie.Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 20.06.2015. Revised 15.07.2015. Accepted: 25.07.2015. УДК 616.43;616-008.9 Особенности осморегулирующей функции почек у больных с сахарным диабетом 1 типаFeatures of the renal osmoregulatory function in patients with type 1 diabetes Е. С. Кузнецова, Е. Н. Кузнецова, В. В. Шухтин, А. И. Гоженко E. S. Kuznetsova, E. N. Kuznetsova, V. V. Shuhtin, A. I. Gozhenko Украинский научно – исследовательский институт медицины транспорта МЗ Украины, ОдессаUkrainian Scientific Research Institute of Transport Medicine MH Ukraine, Odessa Abstract In the context of water - salt load with 0,5% NaCl the osmoregulatory renal function state in 28 patients with type 1 diabetes.It is shown that as the GFR declines to 60 ml \ min the diuresis and % of the discharged liquid increases from 176 ml and 46.1% to 213 ml and 59.5%, while the relative density of urine and osmolality, conversely, decreases. A direct relationship was observed between diuresis and excretion of osmotically active substances (correlation coefficient 0.76 - 0.91), between excretion of osmotically active substances and % of the discharged liquid (0.8 - 0.91), between the relative density and urine osmolality (0.85 - 0.9), between excretion of osmotically active substances (OAS) and glycosuria: thus, maximum excretion of OAS of 224.1 and 345 mOsmol \ hour was combined with glycosuria of 210 and 220 mmol/l.In most of cases (96.4%), glomerular filtration rate averagely increased 2-fold in patients with diabetes 1 type under water - salt stress, rising from 36.9 to 322.5 ml \ min, and renal functional reserve ranged from 36.9 to 439.6%. However, a direct relationship between the RFR and urine osmolality, as well as with an osmotically active substances excretion, was not found.Thus, in type 1 diabetes osmoregulatory renal disorders depend both on the reduction in GFR to 60 ml \ min  and on changes in the functional capacity of nephron`s tubules.  Key words: diabetes mellitus type 1, osmoregulatory function state of kidneys, water- salt load, excretion of osmotically active substances, renal functional reserve. Резюме В условиях водно- солевой нагрузки с 0,5 % NaCl изучено состояние осморегулирующей функции почек у 28 больных с сахарным диабетом 1 типа.Показано, что по мере снижения СКФ до 60 мл\мин, диурез и % выделенной жидкости возрастали с 176 мл  и 46,1 % до 213 мл и 59,5 % , при этом  относительная плотность мочи и осмоляльность, наоборот, снижались. Прямая взаимосвязь наблюдалась между диурезом и экскрецией осмотически активных веществ (коэффициент корреляции 0,76 - 0.91), между экскрецией осмотически активных веществ и % выделенной жидкости (0.8 - 0,91), между  относительной плотностью и осмоляльностью мочи (0.85 до 0,9) и между экскрецией осмотически активных веществ и глюкозурией: так, максимальная экскреция ОАВ 224,1 и 345 мосмоль\час наблюдалась при глюкозурии 210 и 220 ммоль\л.В большинстве случаев (96,4 %) скорость клубочковой фильтрации у больных с СД 1типа в условиях водно-солевой нагрузки возрастала, в среднем в 2 раза, повышаясь от 36,9 до 322,5 мл\мин, а функциональный почечный резерв находился в пределах от 36,9 до 439,6 %. Однако прямой взаимосвязи между  величиной ФПР и осмоляльностью мочи, так же как и с экскрецией осмотически активных веществ не было.Таким образом, при сахарном диабете 1 типа появляются нарушения осморегулирующей функции почек, которые возникают как по мере уменьшения СКФ до 60 мл\мин, так и при изменении функциональной способности канальцев нефронов. Ключевые слова: сахарный диабет 1 тип, осморегулирующая функция почек, водно-солевая проба, эксреция осмотически активных веществ, функциональный почечный резерв