COMPARATIVE ELECTROPHORETIC STUDIES OF LENS PROTEIN ISOLATED FROM PUNTIUS TICTO (HAMILTON 1822) AND RASBORA DANICONUS (HAMILTON 1822)

A comparative electrophoretic study of the eyelens protein of two freshwater fishes viz. Puntius ticto and Rasbora daniconus was carried out. Electrophoretic analyses revealed substantial differences in the polypeptide distribu- tion in both the fishes. SDS-PAGE of water soluble fractions of lens protein showed several bands ranging from 70-13.5 kDa and 74-13.5 kDa in P. ticto and R. daniconus respectively. The insoluble protein fraction was further resolved into the urea soluble lens protein (USLP) and plasma membrane protein fraction(PMP), SDS-PAGE analysis of the USLP fraction showed the presence of 6 bands ranging from 120-13.5 kDa in P. ticto and 114- 13,5 kDa in R. daniconus The PMP fraction of P. ticto resolved into 6 subunits ranging from 74-13.5 kDa. In contrast only two bands corresponding to 29 and 13.5 KDa was observed for the PMP fraction of R. daniconus . The present study demonstrates a complete variation in the protein pattern of the soluble and insoluble protein fraction in these two fishes. The variation in eye lens protein which are considered to be a conserved protein suggest that the R. daniconus had undergone gene duplication in the course of evolution and the two fishes may have evolved differently

LHC Magnet Tests: Operational Techniques and Empowerment for Successful Completion

The LHC magnet tests operation team developed various innovative techniques, particularly since early 2004, to complete the superconductor magnet tests by Feb. 2007. Overall and cryogenic priority handling, rapid on-bench thermal cycling, rule-based goodness evaluation on round-the-clock basis, multiple, mashed web systems are some of these techniques applied with rigour for successful tests completion in time. This paper highlights these operation empowerment tools which had a pivotal role for success. A priority handling method was put in place to enable maximum throughput from twelve test benches, having many different constraints. For the cryogenics infrastructure, it implied judicious allocation of limited resources to the benches. Rapid On-Bench Thermal Cycle was a key strategy to accelerate magnets tests throughput, saving time and simplifying logistics. First level magnet appraisal was developed for 24 hr decision making so as to prepare a magnet further for LHC or keep it on standby. Web based systems (Tests Management and E-Traveller) were other essential ideas to track & coordinate various stages of tests handled by different teams

Study of Water Storage in Existing and Predicted Small Dams on Gadhi and Kasardi Rivers of Panvel Tahsil

Water is an important natural component on the earth. We can’t survive without water. Therefore, it is necessary to conserve and use it properly. Panvel tahsil of Raigad district in Maharashtra receives a lot of rainfall during the monsoons, while the rural areas experience severe water scarcity during the summer. To overcome the water scarcity in the rural areas of Panvel tahsil, this research has been done to build small dams on the Gadhi and Karsardi rivers. For these ten sites have been inspected for construction of small dams on Gadhi and Kasardi rivers. At present, Chota Morbe and Gadheswar are two dams constructed on the Gadhi River. These dams supply 3,40,956 cubic meters of water to Panvel City and rural areas. When small dams have been constructed at the planned site, the water storage will increase to 6,80,052 cubic meters. If Small dams constructed on the Gadhi and Kasardi rivers, water would percolate under the ground and water supply will be available to the farmers in winter and summer season also. Due to increases in the water level, Borewells that supply water to villages will also have plenty of water till the summer season which will eradicate the scarcity of water

Slicing: A New Access to Isolation Preserving Data Publishing

There are several anonymizing techniques like Abstraction, Containerization for isolation preserving small data publishing. The Abstraction loses amount of information for high spatial data. Containerization does not avoid enrollment acknowledgment and does not give clear separation between aspects. We are presenting a technique called slicing which partitions the data both horizontally and vertically. We also show that slicing conserves better data service than abstraction and can be used for enrollment acknowledgment conservation. One more important advantage of slicing is that it can handle high-spatial data. Slicing can be used for aspect acknowledgment conservation and establishing an efficient algorithm for computing the sliced data that obey the l-diversity concern. Our experiments confirm that slicing conserves better service than abstraction and is more active than containerization in loads affecting the conscious aspect and also demonstrate that slicing can be used to avoid enrollment acknowledgment. DOI: 10.17762/ijritcc2321-8169.16045

An unusual presentation of nabothian cyst: a case report

Nabothian cysts are common gynecologic findings and rarely of clinical significance. They are not problematic unless they are sizeable and present secondary symptoms like in this case. Nabothian cysts are usually associated with chronic cervicitis, an inflammatory condition of cervix, and are harmless and usually disappear on their own. Here we report an interesting case of multiple large nabothian cysts presented with continuous, thin & copious watery discharge, a distressing symptom, mimicking genitourinary fistula. This case is reported for the rarity of symptoms of continuous copious thin watery discharge per vaginum. Very rarely nabothian cyst needs hysterectomy as in our case

A Review on Waste Water Treatment in Rural Areas Using Easily Accessible Plants

Background- River water quality and rising water treatment costs are caused by development of land and excessive discharge from storms during the rainy seasons the natural waterfalls on the mountain are blending with the waters. Humans have conserved this water, which eventually turns into sewage and completes the hydrological cycle. Natural streams become polluted as a result of industrialization, urbanization, population development, and other causes that lower their quality, such as sewage from homes, cities, institutions, hospitals, and businesses. The ecosystem and human health are at risk from contaminated streams. As much as possible, natural materials must be used in the process of coagulation-flocculation.Objective- One of the most effective basic chemical treatments is coagulation. Techniques that could be applied to get rid of these pollutants. Natural coagulants have grown in popularity in the water and wastewater treatment industry due to their benefits over chemical coagulants Sector Microbes, plants, and animals all contain natural coagulants. The only treatment available is natural coagulants, but this is insufficient due to the increased limitations on how they work. Actually under these constrained conditions, they are being grown just as successfully as chemical coagulants thanks to technological developments and thorough research.Finding-This study examines a variety of coagulants, particularly those with a natural basis, that are employed in the process coagulation-flocculation of wastewater treatment. This review study also discusses the possibilities of naturebased materials as aids and as coagulants

Cytogenetical studies on the effect of omnacortil on root tip cells of Allium cepa L

Abstract: Omnacortil is steroid, an allopathic drug commonly used as analgesic and suppressant in various diseases. In the present work, onion root tips were subjected with a series of omnacortil concentrations, ranging from 0.2 µg/ml, 0.4 µg/ml, 0.6 µg/ml, 0.8 µg/ml and 1.0 µg/ml for 24 hours for studying its effect on root mitosis in Allium cepa L. The roots were examined in permanent root tip squash preparations stained by the aceto-orcein. This work has confirmed that omnacortil have various effects on chromosomes and induced different mitotic abnormalities and structural aberration of chromosomes. Mitotic index (MI), relative division rate (RDR), relative abnormality rate (RAR) of treated material has been determined. Obtained results showed that all the concentrations of omnacortil showed higher MI and RDR, lower concentrations such as 0.2 µg and 0.4 µg showed higher values over rest of the concentrations and control. Among the subjected concentrations 0.8 µg concentration is most effective and showed highest RAR. Low concentrations of steroids act as stimulants while higher concentrations induced various chromosomal aberrations such as clumping and stickiness, fragmentation, C-mitotic effect, anaphase bridge, chromosomal condensation and contraction, dissolution of chromosome, which clearly showed the mutagenic, clastogenic, antimitotic and cytotoxic effect of omnacortil. This is the first report detailing the effects of omnacortil on chromosomes

Lectotypification of two varietal names in Dioscorea glabra (Dioscoreaceae)

Lectotype of Dioscorea glabra Roxb. var. hastifolia Prain & Burkill and D. glabra Roxb. var. tenuifolia Prain & Burkill are designated here from the syntypes. The error while citing the type for D. serpenticola Hoque & P.K. Mukh. (= D. glabra var. hastifolia) is corrected here

Searches for rare Bs0 and B 0 decays into four muons

Searches for rare Bs0 and B0 decays into four muons are performed using proton-proton collision data recorded by the LHCb experiment, corresponding to an integrated luminosity of 9 fb−1. Direct decays and decays via light scalar and J/ψ resonances are considered. No evidence for the six decays searched for is found and upper limits at the 95% confidence level on their branching fractions ranging between 1.8 × 10−10 and 2.6 × 10−9 are set. [Figure not available: see fulltext.