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The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling
Physarum polycephalum is a well-studied microbial eukaryote with unique experimental attributes relative to other experimental
model organisms. It has a sophisticated life cycle with several distinct stages including amoebal, flagellated, and plasmodial cells. It is
unusual in switching between open and closed mitosis according to specific life-cycle stages. Here we present the analysis of the
genome of this enigmatic and important model organism and compare it with closely related species. The genome is littered with
simple and complex repeats and the coding regions are frequently interrupted by introns with a mean size of 100 bases.
Complemented with extensive transcriptome data, we define approximately 31,000 gene loci, providing unexpected insights into
earlyeukaryoteevolution.Wedescribeextensiveuseofhistidinekinase-basedtwo-componentsystemsandtyrosinekinasesignaling,
the presence of bacterial and plant type photoreceptors (phytochromes, cryptochrome, and phototropin) and of plant-type pentatricopeptide
repeat proteins, as well as metabolic pathways, and a cell cycle control system typically found in more complex eukaryotes.
Our analysis characterizes P. polycephalum as a prototypical eukaryote with features attributed to the last common ancestor of
Amorphea, that is, the Amoebozoa and Opisthokonts. Specifically, the presence of tyrosine kinases inAcanthamoeba and Physarum
as representatives of two distantly related subdivisions ofAmoebozoa argues against the later emergence of tyrosine kinase signaling
in the opisthokont lineage and also against the acquisition by horizontal gene transfe
The behaviour of HEPA filters under high differential pressures
Translated from German (Nuclear Safety Project 1982 Colloquium)SIGLEAvailable from British Library Document Supply Centre- DSC:9091.9F(RISLEY-Trans--5048)T / BLDSC - British Library Document Supply CentreGBUnited Kingdo
The behaviour of HEPA filters under high differential pressures
Translated from German (Nuclear Safety Project 1982 Colloquium)SIGLEAvailable from British Library Document Supply Centre- DSC:9091.9F(RISLEY-Trans--5048)T / BLDSC - British Library Document Supply CentreGBUnited Kingdo