Past and present thermokarst lake dynamics in the Yedoma Ice Complex region of North-Eastern Yakutia

Thermokarst lakes are typical components of the yedoma-alas dominated relief in the coastal lowlands of North- Eastern Yakutia and formed as a result of thawing Late Pleistocene ice-rich Yedoma Ice Complex (IC) deposits. The aim of our study is to estimate thermokarst lake area changes from the early Holocene onwards based on RS data. The decrease of thermokarst lake area from the early Holocene, taking into account total alas depression areas, is as much as 81-83 %. Modern climate warming has led to a general trend of thermokarst lake area decrease. Lake drainage occurs mostly on elevated sites with high Yedoma IC fraction while lake area increase is typical for low-lying areas with a small Yedoma IC fraction. The area increase of thermokarst ponds on flat, boggy yedoma surfaces indicates ice wedge degradation in response to rising summer air temperatures and precipitation

Draft Genome Sequence of Antarctic Methanogen Enriched from Dry Valley Permafrost

A genomic reconstruction belonging to the genus Methanosarcina was assembled from metagenomic data from a methane-producing enrichment of Antarctic permafrost. This is the first methanogen genome reported from permafrost of the Dry Valleys and can help shed light on future climate-affected methane dynamics

Cryogenic Displacement and Accumulation of Biogenic Methane in Frozen Soils

Evidences of highly localized methane fluxes are reported from the Arctic shelf, hot spots of methane emissions in thermokarst lakes, and are believed to evolve to features like Yamal crater on land. The origin of large methane outbursts is problematic. Here we show, that the biogenic methane (δ13C ≤ −71‰) which formed before and during soil freezing is presently held in the permafrost. Field and experimental observations show that methane tends to accumulate at the permafrost table or in the coarse-grained lithological pockets surrounded by the sediments less-permeable for gas. Our field observations, radiocarbon dating, laboratory tests and theory all suggest that depending on the soil structure and freezing dynamics, this methane may have been displaced downwards tens of meters during freezing and has accumulated in the lithological pockets. The initial flux of methane from the one pocket disclosed by drilling was at a rate of more than 2.5 kg C(CH4) m−2 h−1. The age of the methane was 8–18 thousand years younger than the age of the sediments, suggesting that it was displaced tens of meters during freezing. The theoretical background provided the insight on the cryogenic displacement of methane in support of the field and experimental data. Upon freezing of sediments, methane follows water migration and either dissipates in the freezing soils or concentrates at certain places controlled by the freezing rate, initial methane distribution and soil structure

Biodiversity of cryopegs in permafrost

Abstract This study describes the biodiversity of the indigenous microbial community in the sodium-chloride water brines (cryopegs) derived from ancient marine sediments and sandwiched within permafrost 100-120,000 years ago after the Arctic Ocean regression. Cryopegs remain liquid at the in situ temperature of À9 to À11°C and make up the only habitat on the Earth that is characterized by permanently subzero temperatures, high salinity, and the absence of external influence during geological time. From these cryopegs, anaerobic and aerobic, spore-less and spore-forming, halotolerant and halophilic, psychrophilic and psychrotrophic bacteria, mycelial fungi and yeast were isolated and their activity was detected below 0°C

Ciliates from ancient permafrost: assessment of cold resistance of the resting cysts

There is evidence that resting cysts of soil ciliates and numerous taxa of other protists can survive in permafrost for thousands of years at subzero temperatures; however, our knowledge about mechanisms of long term cryobiosis remains incomplete. In order to better understand the means by which ancient cysts survive, we investigated resistance to cyclical supercooling stress of resting cysts of the soil ciliate Colpoda steinii (Colpodida, Ciliophora). Three clonal strains were used for comparison, isolated from Siberian tundra soil, ancient Holocene (5-7,000 y) and late Pleistocene (32-35,000 y) permafrost sediments. To determine the viability of the ancient and contemporary ciliate cysts we improved and validated a cultivation-independent method of vital fluorescent staining with a combination of two nucleic acid binding dyes, acridine orange and propidium iodide. The viability of Colpoda steinii cysts during low-temperature experiments was measured using both the proposed vital fluorescent staining method and standard germination test. Our results indicate that the dual-fluorescence technique is a more accurate, rapid, and efficient method for estimating cyst viability. We found that cysts of ancient ciliates display lower tolerance to the impact of cyclical cold compared to cysts of contemporary ciliates from Siberian permafrost affected soils

Frozen Zoo: a collection of permafrost samples containing viable protists and their viruses

International audiencePermafrost, frozen ground cemented with ice, occupies about a quarter of the Earth’s hard surface and reaches up to 1000 metres depth. Due to constant subzero temperatures, permafrost represents a unique record of past epochs, whenever it comes to accumulated methane, oxygen isotope ratio or stored mummies of animals. Permafrost is also a unique environment where cryptobiotic stages of different microorganisms are trapped and stored alive for up to hundreds of thousands of years. Several protist strains and two giant protist viruses isolated from permafrost cores have been already describe

Geomorphological and Climatic Drivers of Thermokarst Lake Area Increase Trend (1999–2018) in the Kolyma Lowland Yedoma Region, North-Eastern Siberia

Thermokarst lakes are widespread in Arctic lowlands. Under a warming climate, landscapes with highly ice-rich Yedoma Ice Complex (IC) deposits are particularly vulnerable, and thermokarst lake area dynamics serve as an indicator for their response to climate change. We conducted lake change trend analysis for a 44,500 km2 region of the Kolyma Lowland using Landsat imagery in conjunction with TanDEM-X digital elevation model and Quaternary Geology map data. We delineated yedoma–alas relief types with different yedoma fractions, serving as a base for geospatial analysis of lake area dynamics. We quantified lake changes over the 1999–2018 period using machine-learning-based classification of robust trends of multi-spectral indices of Landsat data and object-based long-term lake detection. We analyzed the lake area dynamics separately for 1999–2013 and 1999–2018 periods, including the most recent five years that were characterized by very high precipitation. Comparison of drained lake basin area with thermokarst lake extents reveal the overall limnicity decrease by 80% during the Holocene. Current climate warming and wetting in the region led to a lake area increase by 0.89% for the 1999–2013 period and an increase by 4.15% for the 1999–2018 period. We analyzed geomorphological factors impacting modern lake area changes for both periods such as lake size, elevation, and yedoma–alas relief type. We detected a lake area expansion trend in high yedoma fraction areas indicating ongoing Yedoma IC degradation by lake thermokarst. Our concept of differentiating yedoma–alas relief types helps to characterize landscape-scale lake area changes and could potentially be applied for refined assessments of greenhouse gas emissions in Yedoma regions. Comprehensive geomorphological inventories of Yedoma regions using geospatial data provide a better understanding of the extent of thermokarst processes during the Holocene and the pre-conditioning of modern thermokarst lake area dynamics

Two metagenomes from late pleistocene northeast siberian permafrost

The present study reports metagenomic shotgun sequencing of microbial communities of two ancient permafrost horizons of the Russian Arctic. Results demonstrate a significant difference in microbial community structure of the analyzed samples in general and microorganisms of the methane cycle in particular

Bacteria in Permafrost

Significant numbers of viable ancient microorganisms are known to be present within the permafrost. They have been isolated in both polar regions from the cores up to 400 m deep and ground temperatures of -27 C. The age of the cells corresponds to the longevity of the permanently frozen state of the soils, with the oldest cells dating back to {approx}3 million years in the Arctic, and {approx}5 million years in the Antarctic. They are the only life forms known to have retained viability over geological time. Thawing of the permafrost renews their physiological activity and exposes ancient life to modern ecosystems. Thus, the permafrost represents a stable and unique physicochemical complex, which maintains life incomparably longer than any other known habitats. If we take into account the depth of the permafrost layers, it is easy to conclude that they contain a total microbial biomass many times higher than that of the soil cover. This great mass of viable matter is peculiar to permafrost only

Two metagenomes from late pleistocene northeast siberian permafrost

The present study reports metagenomic shotgun sequencing of microbial communities of two ancient permafrost horizons of the Russian Arctic. Results demonstrate a significant difference in microbial community structure of the analyzed samples in general and microorganisms of the methane cycle in particular