RESEARCH AND REVIEWS: JOURNAL OF PHARMACOLOGY AND TOXICOLOGICAL STUDIES Cytotoxicity analysis by MTT assay of isolated Gossypol from Bt and Non-Bt Cotton Seeds on HeLa Cell Lines

ABSTRACT The present paper deals with study of In-vitro cytotoxicity effect of isolated gossypol from Bt and Non-Bt cotton seeds on HeLa cell lines. Gossypol is a polyphenolic binaphthyl diadehyde natural yellow colored pigment. It is not only resistance substance for cotton plant's selfdefense system against insect pests and possibly some diseases but also an important phytochemical compound of immense interest due to its several biological properties including anti-cancer,anti-HIV,antioxidation, antimicrobial and as male contraceptive. During this study gossypol exhibited broad spectrum of anti-cancer activity against the HeLa cell lines. The cytotoxicity effect of gossypol was detemined by MTT (3-(4-,5-dimethylthiazolyl-2)-2,5-dipheniltetrazolium bromide) assay. Gossypol from Bt and Non-Bt cotton seeds has shown dose dependent cytotoxicity effect against HeLa cell lines. In-vitro screening of the gossypol showed potential cytotoxic activity against HeLa cell lines. Mortality rate of 11.5884% and 22.6058% with 3µg/1µl concentration of isolated gossypol from Bt and Non-Bt cotton seed extracts was observed on HeLa cell lines respectively. Hence the inhibitory concentration at 50% (IC50) was fixed at 3µg/1µl of gossypol for HeLa cells. The standard anti-cancer drug Doxorubicin (1mg/ml) was also used in this study to confirm anti-cancer activity of gossypol isolated from Bt and Non-Bt cotton seed with 1.7828% cell viability. The present study confirms the mild toxic effect of gossypol on HeLa cell lines and can preferably be used as anti-cancerous drug in combination with other natural similar compounds to replace the synthetic chemical drugs for fewer side effects

Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae

Objective(s): To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho0 yeast cells. Materials and Methods: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes in cytosol/mitochondrial compartments, reactive oxygen species production and respiratory control ratio. Results: Wild-type yeast grown on glycerol exhibited heightened sensitivity to cisplatin than yeast grown on glucose. Cisplatin (100 μM), although significantly reduced the growth of wild- type cells, only slightly altered the growth rate of Rho0 cells. Cisplatin treatment decreased both pHcyt and pHmit to a similar extent without affecting the pH difference. Cisplatin dose-dependently increased the oxidative stress in wild-type, but not in respiration-deficient Rho0 strain. Cisplatin decreased the respiratory control ratio. Conclusion: These results suggest that cisplatin toxicity is influenced by the respiratory capacity of the cells and the intracellular oxidative burden. Although cisplatin per se slightly decreased the respiration of yeast cells grown in glucose, it did not disturb the mitochondrial chemiosmotic gradient