The Past in the Present: How Innovative History-making Shapes Our Many Mountains

Kathryn Newfont, Professor of History at Mars Hill University, will reflect on how collaborations such as the Madison County “Forever Free” project extension can shape our understanding not only of the Appalachian region’s complex past, but also of its multi-faceted present. Drew Reisinger’s efforts to unearth the history of chattel slavery in the public records under his care set a new standard for the nation. From the beginning Reisinger emphasized the importance of this historical work to the present-day citizens of Buncombe County, who elect the Register of Deeds. Now, in another Appalachian innovation, educators and students with Mars Hill University’s Public History Program have extended this remarkable effort into neighboring Madison County. Newfont will reflect on the potential this sort of work has for transforming our understanding of slavery in the southern mountain region and beyond. She will consider ways this collaboration and others like it can not only extend our grasp of the Appalachian region’s “Many Mountains” past, but also shapes our understandings of its complex “Many Mountains” present

Soil Fungi Alter Interactions Between the Invader Centaurea Maculosa and North American Natives

Soil microbes may affect the way exotic invasive plants interact with native neighbors. We investigated the effects of soil fungi on interactions between the invasive weed Centaurea maculosa (spotted knapweed) and six species native to the intermountain prairies of the northwestern United States. We also compared the effect of C. maculosa on the composition of the soil microbial community to that of the native species. In the field, fungicide (Benomyl) reduced AM mycorrhizal colonization of C. maculosa roots by \u3e80%. Fungicide did not significantly reduce non-AM fungi. When grown alone, the biomass of C. maculosa was not affected by the fungicide application. However, depending on the combination of native competitor and fungicide, C. maculosa biomass varied from 10-fold decreases to 1.9-fold increases. In untreated soils, C. maculosa grew larger in the presence of Festuca idahoensis or Koeleria cristata than when alone. When fungicide was applied these positive effects of Festuca and Koeleria on C. maculosa did not occur. A third native grass, Pseudoroegneria spicata, had much stronger competitive effects on C. maculosa than Festuca or Koeleria, and fungicide reduced the competitive effects of Pseudoroegneria. Fungicide increased Centaurea biomass when competing with the forb Gallardia aristata. However, fungicide did not affect the way two other forbs; Achillea millefolium and Linum lewisii, interacted with C. maculosa. Rhizosphere microbial communities in the root zones of the three native bunchgrass species differed from that of C. maculosa. However, despite the strong effects of soil fungi in field interactions and differences in microbial community composition, soil biota from different plant rhizospheres did not affect the growth of C. maculosa in the absence of native competitors in greenhouse experiments. Our results suggest that successful invasions by exotic plant species can be affected by complex and often beneficial effects of local soil microbial communities. These effects were not manifest as simple direct effects, but become apparent only when native plants, invasive plants, and soil microbial communities were interacting at the same time

A Layered History: Interpreting Cultural Resources at Sesquicentennial State Park

Sesquicentennial State Park is one of the most popular state parks in South Carolina and is well-known in the Columbia metropolitan area as a refuge from the hustle and bustle of the urban scene. Driving through its entrance gates from busy Two Notch Road, visitors find themselves immediately in the midst of a pine forest. Past the ranger’s kiosk a winding road follows the contours of the gently rolling terrain, offering occasional glimpses of a mysterious fire tower, an evocative two-story log house, and eventually the open vista of a large lake with white concrete buildings and lawns along the shore. This beguiling patch of nature in the midst of the city has long been a place for recreation and for learning natural history in an “outdoor classroom.” Although an historical plaque notes that the park was established by the Sesquicentennial Commission, there is little at the modern park to help visitors see that the site has both natural history and a deep human history. (A couple of recently placed waysides now explain the role of the Civilian Conservation Corps in the 1930s and a civil rights challenge in the 1960s.) This report presents some preliminary findings on the human history of the park – as well as the history of the site before it became a park. The report is divided into seven sections. The first section is the park’s current mission statement. Sections two and three present a set of “statements of significance” and “primary interpretive themes” which seek to identify important patterns of history at the site as ways to make the past meaningful to public audiences. The fourth and largest section offers fresh research on the history of Sesquicentennial State Park by “periodizing” it into five eras: the pre-park history, the role of the Sesquicentennial Commission of 1936 in creating the park, the management of the South Carolina Commission of Forestry, the ten-year struggle to desegregate South Carolina’s state parks, and the management of the South Carolina State Park Service, the current steward of the park. Section five offers ideas for how this history might be communicated to visitors in a cost-effective manner, using strategically placed QR codes in public areas and along existing walking trails. Because this is a preliminary report on park history, section six contains suggestions for further research to help guide future researchers, within or beyond the South Carolina State Park Service. The research team discovered much new information but much more can be uncovered. Lastly, section seven is an appendix with an historical timeline, bibliographies, additional information on individuals significant in the pre-park era, and a list of people who worked to establish a museum on the history of Columbia as one legacy of the Sesquicentennial commemoration.https://scholarcommons.sc.edu/pubhist_books/1004/thumbnail.jp

Accurate Inference of Subtle Population Structure (and Other Genetic Discontinuities) Using Principal Coordinates

Accurate inference of genetic discontinuities between populations is an essential component of intraspecific biodiversity and evolution studies, as well as associative genetics. The most widely-used methods to infer population structure are model-based, Bayesian MCMC procedures that minimize Hardy-Weinberg and linkage disequilibrium within subpopulations. These methods are useful, but suffer from large computational requirements and a dependence on modeling assumptions that may not be met in real data sets. Here we describe the development of a new approach, PCO-MC, which couples principal coordinate analysis to a clustering procedure for the inference of population structure from multilocus genotype data.PCO-MC uses data from all principal coordinate axes simultaneously to calculate a multidimensional "density landscape", from which the number of subpopulations, and the membership within subpopulations, is determined using a valley-seeking algorithm. Using extensive simulations, we show that this approach outperforms a Bayesian MCMC procedure when many loci (e.g. 100) are sampled, but that the Bayesian procedure is marginally superior with few loci (e.g. 10). When presented with sufficient data, PCO-MC accurately delineated subpopulations with population F(st) values as low as 0.03 (G'(st)>0.2), whereas the limit of resolution of the Bayesian approach was F(st) = 0.05 (G'(st)>0.35).We draw a distinction between population structure inference for describing biodiversity as opposed to Type I error control in associative genetics. We suggest that discrete assignments, like those produced by PCO-MC, are appropriate for circumscribing units of biodiversity whereas expression of population structure as a continuous variable is more useful for case-control correction in structured association studies

Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cellular architecture or endogenous fluorescence. PACT (passive CLARITY technique) and PARS (perfusion-assisted agent release in situ) use tissue-hydrogel hybrids to stabilize tissue biomolecules during selective lipid extraction, resulting in enhanced clearing efficiency and sample integrity. Furthermore, the macromolecule permeability of PACT- and PARS-processed tissue hybrids supports the diffusion of immunolabels throughout intact tissue, whereas RIMS (refractive index matching solution) grants high-resolution imaging at depth by further reducing light scattering in cleared and uncleared samples alike. These methods are adaptable to difficult-to-image tissues, such as bone (PACT-deCAL), and to magnified single-cell visualization (ePACT). Together, these protocols and solutions enable phenotyping of subcellular components and tracing cellular connectivity in intact biological networks

Canadian Led X-ray Polarimeter Mission CXP

We propose a Canadian-led X-ray Polarimetry Mission (CXP), to include a scattering X-ray Polarimeter and sensitive All-Sky X-ray Monitor (ASXM). Polarimetry would provide a new observational window on black holes, neutron stars, accretion disks and jets, and the ASXM would offer sensitive monitoring of the volatile X-ray sky. The envisioned polarimeter consists of a hollow scattering beryllium cone surrounded by an annular proportional counter, in a simple and elegant design that is reliable and low-risk. It would be sensitive in the 6-30 keV band to approx. 3% polarization in approx. 30 Galactic sources and 2 AGN in a baseline 1-yr mission, and have sensitivity greater than 10 times that of the previous X-ray polarimeter flown (NASA's OSO-8, 1975-78) for most sources. This X-ray polarimeter would tackle questions like, Do black holes spin?, How do pulsars pulse?, What is the geometry of the magnetic field in accreting neutron stars? Where and how are jets produced in microquasars and AGN?, What are the geometries of many of the most famous accretion-disk systems in the sky? This will be done using a novel and until-now unexploited technique that will greatly broaden the available observational phase space of compact objects by adding to timing and spectroscopy observations of polarization fraction and position angle as a function of energy. The All-Sky X-ray Monitor would scan for transients, both as potential targets for the polarimeter but also as a service to the worldwide astronomical community. The entire CXP mission could be flown for $40- 60M CDN, according to estimates by ComDev International, and could be built entirely in Canada. It would fall well within the CSA's SmallSat envelope and would empower the growing and dynamic Canadian High-Energy Astrophysics community with world leadership in a potentially high impact niche area