Proteins from morphologically differentiated neuroblastoma cells promote tubulin polymerization

Clonal cells (N18) of the mouse neuroblastoma C-1300 can be induced to undergo a morphological differentiation characterized by the outgrowth of very long neurites (> 150 microns) that contain many microtubules. Because the marked increase in the number and length of microtubules is apparently not due to an increase in the concentration of tubulin subunits, the possible role of additional macromolecules in the regulation of tubulin polymerization during neurite formation by N18 cells was examined. Using an in vitro system where the polymerization of low concentrations (< 4 mg/ml) of purified brain tubulin requires microtubule-associated proteins (MAPs), high-speed supernates (250,000 g) from neuroblastoma and glioma cells were assayed for their ability to replace MAPs in the polymerization of brain tubulin. Only the supernates from "differentiated" N18 cells were polymerization competent. Electron microscope observations of these supernates failed to demonstrate the presence of nucleation structures (rings or disks). The active factor(s) sedimented at approximately 7S on sucrose gradient centrifugation and eluted from 4B Sepharose in the region of 170,000 mol wt proteins. Furthermore, the inactive supernates from other cells did not inhibit polymerization when tested in the presence of limiting MAPs. Thus, microtubule formation accompanying neurite outgrowth in neuroblastoma cells appears to be regulated by the presence of additional macromolecular factor(s) that may be functionally equivalent to the MAPs found with brain microtubules

Interferometric length metrology for the dimensional control of ultra-stable Ring Laser Gyroscopes

We present the experimental test of a method for controlling the absolute length of the diagonals of square ring laser gyroscopes. The purpose is to actively stabilize the ring cavity geometry and to enhance the rotation sensor stability in order to reach the requirements for the detection of the relativistic Lense-Thirring effect with a ground-based array of optical gyroscopes. The test apparatus consists of two optical cavities 1.32 m in length, reproducing the features of the ring cavity diagonal resonators of large frame He-Ne ring laser gyroscopes. The proposed measurement technique is based on the use of a single diode laser, injection locked to a frequency stabilized He-Ne/Iodine frequency standard, and a single electro-optic modulator. The laser is modulated with a combination of three frequencies allowing to lock the two cavities to the same resonance frequency and, at the same time, to determine the cavity Free Spectral Range (FSR). We obtain a stable lock of the two cavities to the same optical frequency reference, providing a length stabilization at the level of 1 part in $10^{11}$, and the determination of the two FSRs with a relative precision of 0.2 ppm. This is equivalent to an error of 500 nm on the absolute length difference between the two cavities

The Origanum Collection of Gaetano Savi (1769-1844) in the Herbarium Horti Pisani (PI)

The Origanum Collection of Gaetano Savi (1769-1844) in the Herbarium Horti Pisani (PI). The collection of exsiccata of the genus Origanum, studied by Gaetano Savi and kept in the Herbarium Horti Pisani (PI) represents a valuable document of Savi's work as a taxonomist. It provides useful news for researchers of systematics and history of botany. The collection is enriched by some original drawings and includes the lectotypes of Origanum confertum Savi and O. fortuitum Savi

Lectotypification of two Origanum names (Lamiaceae) described by Gaetano Savi

Origanum confertum Savi and O. fortuitum Savi are typified on herbarium specimens kept in PI. These taxa are identifiable, as heterotypic synonyms, with O. majorana L. and O. xpaniculatum W.D.J.Koch, respectively

Developing fiber lasers with Bragg reflectors as deep sea hydrophones

The present paper will discuss the work in progress at the Department of Physics of the University of Pisa in collaboration with the IFAC laboratory of CNR in Florence to develop pressure sensors with outstanding sensitivity in the acoustic and ultrasonic ranges. These devices are based on optically-pumped fiber lasers, where the mirrors are Bragg gratings written into the fiber core

Review of "Proteins of the Cerebrospinal Fluid" (2(nd )Edition) by Edward J. Thompson

This book on cerebrospinal fluid (CSF) proteins is primarily focused on immunoglobulins. The book was written as an extension of a meeting on multiple sclerosis to provide a more extensive consideration of the CSF

Geometrical scale-factor stabilization of square cavity ring laser gyroscopes

Large frame ring laser gyros performances are ultimately limited by the instabilities of their geometrical parameters. We present the experimental activity on the GP2 ring laser gyro. GP2 is a ring laser gyro devoted to develop advanced stabilization techniques of the ring cavity geometrical scale-factor. A method based on optical interferometry has been developed for canceling the deformations of the resonator. The method is based on the measurement and stabilization of the absolute length of the cavity perimeter and of the resonators formed by the opposite cavity mirrors. The optical frequency reference in the experiment is an iodine-stabilized He-Ne laser, with a relative frequency stability of 10-11. The measurement of the absolute length of the two resonators has been demonstrated up to now on a test bench. We discuss the experimental results on GP2: the present performances as a ring laser gyro and the stabilization scheme to be implemented in the near future