Improvement of data collection and modelling techniques of disaggregate logit models (a case study on work trip mode-choice behavior in metro Manila)

Thesis--University of Tsukuba, D.So.Ec.Pl.(A), no. 611, 1989. 3. 2

Expression of \u3cem\u3espoT\u3c/em\u3e in \u3cem\u3eBorrelia burgdorferi\u3c/em\u3e during Serum Starvation

Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted by the tick Ixodes scapularis. A 2.9-kb fragment containing a putative spoT gene was isolated from B. burgdorferi genomic DNA by PCR amplification and cloned into a pBAD24 vector. The cloned gene complemented Escherichia coli mutant strain CF1693, which contains deletions of both the relA and spoT genes. The spoT gene in E. coli encodes a bifunctional enzyme capable of synthesizing and degrading (p)ppGpp, which mediates the stringent response during carbon source starvation. B. burgdorferi has been reported to have a stress response to serum starvation. Thin-layer chromatography was used to detect (p)ppGpp extracted from H3 32PO4-labeled B. burgdorferi cells starved for serum in RPMI. B. burgdorferi spoT gene expression was characterized during fatty acid starvation. Northern analysis of spoT revealed detectable message at 2.5 min of starvation in RPMI. Expression of spoT during serum starvation increased _6-fold during the 30 min that starvation conditions were maintained. Further, expression of spoT decreased when serum was added to serum-starved cells. Reverse transcriptase PCR (RT-PCR) was used to detect spoT mRNA from ~106 cells starved for serum in RPMI for 2.5 to 30 min or incubated in tick saliva for 15 min. Northern blot analysis suggests that spoT transcript was ~900 nucleotides in length. RT-PCR amplification of the transcript using several sets of primers confirmed this finding. Additionally, a truncated clone containing only the first 950 bp of the 2,001-bp spoT open reading frame was able to complement E. coli CF1693. The data suggest that B. burgdorferi exhibits a stringent response to serum starvation and during incubation in tick saliva

Assessing the Capacities of Cooperatives to Manage a Business: The Case of St. Marcos Multi-Purpose Cooperative

Cooperatives play a significant role in the Comprehensive Agrarian Reform Program. Besides serving as conduits of support services to farmers, they are also expected to implement agri-based projects that will help increase income and productivity of its members and other community members. The St. Marcos Multi-Purpose Cooperative (SMMPC), which operates in Barangay Gapasan in the Municipality of Magsaysay, Occidental Mindoro submitted a proposal to the Department of Agrarian Reform (DAR) for funding. The proposed project intends to increase farm productivity and income and entails renting out of common service facilities (CSFs) such as a 35-HP four-wheel tractor and combined harvester-thresher by SMMPC. The CSFs will be provided for free by the DAR, but the cooperative is required to have an equity. Given the current, weak financial status and management capabilities of cooperatives in the Philippines, the question of whether SMMPC can operate its proposed project and sustain its operation arose. This paper assessed the capacity of the cooperative to manage and sustain the operation of the project. Furthermore, the relevance, acceptability, and estimated returns of the proposed project were also examined. Data was gathered through focus group discussions with the cooperative members and barangay residents who are not affiliated with the organization. Key informant interviews with the DAR personnel and gathering of secondary data were also conducted. Results indicated that the SMMPC has a stable financial base and its officers are capable of running the proposed project. Moreover, the high demand for the CSFs assures that SMMPC will be profitable

Repression of the Glucocorticoid Receptor Aggravates Acute Ischemic Brain Injuries in Adult Mice.

Strokes are one of the leading causes of mortality and chronic morbidity in the world, yet with only limited successful interventions available at present. Our previous studies revealed the potential role of the glucocorticoid receptor (GR) in the pathogenesis of neonatal hypoxic-ischemic encephalopathy (HIE). In the present study, we investigate the effect of GR knockdown on acute ischemic brain injuries in a model of focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO) in adult male CD1 mice. GR siRNAs and the negative control were administered via intracerebroventricular (i.c.v.) injection 48 h prior to MCAO. The cerebral infarction volume and neurobehavioral deficits were determined 48 h after MCAO. RT-qPCR was employed to assess the inflammation-related gene expression profiles in the brain before and after MCAO. Western Blotting was used to evaluate the expression levels of GR, the mineralocorticoid receptor (MR) and the brain-derived neurotrophic factor/tropomyosin receptor kinase B (BDNF/TrkB) signaling. The siRNAs treatment decreased GR, but not MR, protein expression, and significantly enhanced expression levels of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) in the brain. Of interest, GR knockdown suppressed BDNF/TrkB signaling in adult mice brains. Importantly, GR siRNA pretreatment significantly increased the infarction size and exacerbated the neurobehavioral deficits induced by MCAO in comparison to the control group. Thus, the present study demonstrates the important role of GR in the regulation of the inflammatory responses and neurotrophic BDNF/TrkB signaling pathway in acute ischemic brain injuries in adult mice, revealing a new insight into the pathogenesis and therapeutic potential in acute ischemic strokes

Evidence for hyperacute rejection of human liver grafts: The case of the canary kidneys

Sequential liver and kidney transplantation from the same donor was performed in 2 patients. The kidney in Patient 1, which was transplanted after the liver, was hyperacutely rejected and removed 6 hours later. The first liver as well as another liver transplanted 3 days later developed widespread hemorrhagic necrosis. Although the cytotoxic crossmatch of preoperative recipient serum with both donors was negative, patchy widespread IgM and C(1q) deposits were found in all 3 organs. In Patient 2, who had a strongly positive cytotoxic crossmatch with his donor, the liver suffered a massive but reversible injury, while the kidney never functioned. Both patients developed a coagulopathy a few minutes after liver revascularization. The kidneys in these cases had served like the canaries which miners once used to detect a hostile environment and their presence made more understandable how an indolent version of hyperacute rejection of the liver can take place

Limits, Transparency, and Board Independence Against Tax Avoidance

Taxes are levied by the government primarily for public service purposes (De Leon & De Leon, 2016).However, the high tax rates and narrow tax base imposed in the Philippines become burdensome for taxpayers and also decrease total government revenue collections (Diokno, 2008). It is shown in this study that foreign ownership and customer concentration both increase the levels of corporate tax avoidance practiced by firms while board independence decreases the same. Hence, we do not support the government’s plan to ease foreign ownership restrictions through the amendment of the Constitution (Romualdez, 2017) because easing the current restrictions may entail decreased government revenue. Additionally, we recommend that the International Accounting Standards Board (IASB) strengthen the enforcement of the International Financial Reporting Standards 8 (IFRS 8). In this case, increased transparency in disclosures is needed to help the government prevent the use of tax havens by major conglomerates. Lastly, we support the Securities and Exchange Commission’s (SEC) recommendation to have a certain minimum threshold on the proportion of independent directors in PSElisted firms on the basis that doing so will reduce levels of tax avoidance within these firms

Reactivity of monolayer V\u3csub\u3e2\u3c/sub\u3eO\u3csub\u3e5\u3c/sub\u3e films on TiO\u3csub\u3e2\u3c/sub\u3e(110) produced via the oxidation of vapor-deposited vanadium

The growth, and reactivity of monolayer V2O5 films supported on TiO2(110) produced via the oxidation of vapor-deposited vanadium were studied using X-ray photoelectron spectroscopy (XPS) and temperature programmed desorption (TPD). Oxidation of vapor-deposited vanadium in 10-7 Torr of O2 at 600 K produced vanadia films that contained primarily V+3, while oxidation in 10-3 Torr at 400 K produced films that contained primarily V+5. The reactivity of the supported vanadia layers for the oxidation of methanol to formaldehyde was studied using TPD. The activity for this reaction was found to be a function of the oxidation state of the vanadium cations in the film

Evolution and stabilization of subnanometric metal species in confined space by in situ TEM

Understanding the behavior and dynamic structural transformation of subnanometric metal species under reaction conditions will be helpful for understanding catalytic phenomena and for developing more efficient and stable catalysts based on single atoms and clusters. In this work, the evolution and stabilization of subnanometric Pt species confined in MCM-22 zeolite has been studied by in situ transmission electron microscopy (TEM). By correlating the results from in situ TEM studies and the results obtained in a continuous fix-bed reactor, it has been possible to delimitate the factors that control the dynamic agglomeration and redispersion behavior of metal species under reaction conditions. The dynamic reversible transformation between atomically dispersed Pt species and clusters/nanoparticles during CO oxidation at different temperatures has been elucidated. It has also been confirmed that subnanometric Pt clusters can be stabilized in MCM-22 crystallites during NO reduction with CO and H2

Factors affecting farmers’ adoption of natural farming technologies in New Albay, Maragusan, Compostela Valley, Philippines

In the Philippines, the sustainability of natural resources and the profitability of farming has become a major concern for smallholder farmers. To address these concerns, government agencies and the private sector are delivering workshops on natural farming technologies to farmers. Training includes the preparation of different concoctions such as indigenous micro-organisms (IMO), fermented fruit juice (FFJ) and fermented plant juice (FPJ), fish amino acid (FAA), oriental herbal juice (OHN) and calcium phosphate (Caphos). Other natural farm technologies include vermiculture. The products from the training are applied to the farmer’s own farms with any surplus products sold to other farmers. In Maragusan, Compostela Valley, Philippines, a group of smallholder farmers have successfully produced organic fertilizers as a result of the training provided to them. Initially, while training was provided to the group to facilitate the marketing of the fresh vegetable crops they produced, the skills learnt were readily transferable. This study discusses the development of the New Albay cluster and factors leading to its decline as a marketing cluster. This study will also explore the costs and returns associated with the application of organic fertilizer to vegetable crops in comparison to conventional farming practices. Results show that the cost of production arising from the use of natural farming technologies is lower than conventional farming