New cationic polyprenyl derivative proposed as a lipofecting agent

Cationic linear poly-cis-isoprenoid prepared from natural plant polyprenol in a mixture with dioleyl phosphatidylethanolamine was found to be an effective lipofection agent for eukaryotic cells. The transfecting activity is related to the poly-cis structure of the polyprenyl chain

Diagnostic accuracy of liquid biopsy in endometrial cancer

Background: Liquid biopsy is a minimally invasive collection of a patient body fluid sample. In oncology, they offer several advantages compared to traditional tissue biopsies. However, the potential of this method in endometrial cancer (EC) remains poorly explored. We studied the utility of tumor educated platelets (TEPs) and circulating tumor DNA (ctDNA) for preoperative EC diagnosis, including histology determination. Methods: TEPs from 295 subjects (53 EC patients, 38 patients with benign gynecologic conditions, and 204 healthy women) were RNA-sequenced. DNA sequencing data were obtained for 519 primary tumor tissues and 16 plasma samples. Artificial intelligence was applied to sample classification. Results: Platelet-dedicated classifier yielded AUC of 97.5% in the test set when discriminating between healthy subjects and cancer patients. However, the discrimination between endometrial cancer and benign gynecologic conditions was more challenging, with AUC of 84.1%. ctDNA-dedicated classifier discriminated primary tumor tissue samples with AUC of 96% and ctDNA blood samples with AUC of 69.8%. Conclusions: Liquid biopsies show potential in EC diagnosis. Both TEPs and ctDNA profiles coupled with artificial intelligence constitute a source of useful information. Further work involving more cases is warranted.publishedVersio

Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP)

The capability of environmental microorganisms to biodegrade simazine—an active substance of 2-chloro-s-triazine herbicides (pesticide waste since 2007)—was assessed. An enormous metabolic potential of microorganisms impels to explore the possibilities of using them as an alternative way for thermal and chemical methods of utilization. First, the biotope rich in microorganisms resistant to simazine was examined. Only the higher dose of simazine (100 mg/l) had an actual influence on quantity of bacteria and environmental fungi incubated on substrate with simazine. Most simazine-resistant bacteria populated activated sludge and biohumus (vermicompost); the biggest strain of resistant fungi was found in floral soil and risosphere soil of maize. Compost and biohumus were the sources of microorganisms which biodegraded simazine, though either of them was the dominant considering the quantity of simazine-resistant microorganisms. In both cases of periodic culture (microorganisms from biohumus and compost), nearly 100% of simazine (50 mg/l) was degraded (within 8 days). After the repeated enrichment culture with simazine, the rate of its degradation highly accelerated, and just after 24 h, the significant decrease of simazine (20% in compost and 80% in biohumus) was noted. Although a dozen attempts of isolating various strains responsible for biodegradation of simazine from compost and biohumus were performed, only the strain identified as Arthrobacter urefaciens (NC) was obtained, and it biodegraded simazine with almost 100% efficiency (within 4 days)

Mechanical thrombectomy in acute stroke – Five years of experience in Poland

Objectives Mechanical thrombectomy (MT) is not reimbursed by the Polish public health system. We present a description of 5 years of experience with MT in acute stroke in Comprehensive Stroke Centers (CSCs) in Poland. Methods and results We retrospectively analyzed the results of a structured questionnaire from 23 out of 25 identified CSCs and 22 data sets that include 61 clinical, radiological and outcome measures. Results Most of the CSCs (74%) were founded at University Hospitals and most (65.2%) work round the clock. In 78.3% of them, the working teams are composed of neurologists and neuro-radiologists. All CSCs perform CT and angio-CT before MT. In total 586 patients were subjected to MT and data from 531 of them were analyzed. Mean time laps from stroke onset to groin puncture was 250±99min. 90.3% of the studied patients had MT within 6h from stroke onset; 59.3% of them were treated with IV rt-PA prior to MT; 15.1% had IA rt-PA during MT and 4.7% – emergent stenting of a large vessel. M1 of MCA was occluded in 47.8% of cases. The Solitaire device was used in 53% of cases. Successful recanalization (TICI2b–TICI3) was achieved in 64.6% of cases and 53.4% of patients did not experience hemorrhagic transformation. Clinical improvement on discharge was noticed in 53.7% of cases, futile recanalization – in 30.7%, mRS of 0–2 – in 31.4% and mRS of 6 in 22% of cases. Conclusion Our results can help harmonize standards for MT in Poland according to international guidelines

The impact of precipitation on state of the slopes surface and thermal activity of the mine waste dump – preliminary study

Precipitation, especially with a high intensity, affects the condition of mining waste dumps. The article presents the results of research aimed at determining the impact of rain on water erosion on the slopes of a coal waste dump and its thermal state. Preliminary tests of the condition of two slopes of the coal waste dump in Libiąż (Poland) undertaken in the frameworks of the TEXMIN project was carried out using modern geodesy techniques (low-ceiling photogrammetry and TLS terrestrial laser scanning). The current geometry of the slope surface was faithfully reproduced in the form of a cloud of points with known coordinates x, y, z. The thermal state within the analyzed slopes of the dump was also assessed. Based on thermography studies and measurements of temperature and gas concentrations inside the object, two zones of thermal activity were located on one of the examined slopes. The test results constitute the initial state against which the results of further tests will be compared. This will allow to determine the influence of precipitation on the amount of water erosion and the thermal state of the dump in a specific time period

The impact of precipitation on the groundwater of coal waste dump

The aim of the study was to assess the effect of climate change, mainly higher and lower precipitation, on the intensity of the impact of a coal waste dump on groundwater. The analysis used meteorological data for the Katowice region in 2002-2020 as well as data on the height of the groundwater table in the vicinity of the coal waste dump, and data on physicochemical parameters and chemical composition of groundwater in 2004-2020. Based on the analyses, it was found that the periods of drought in the Silesian Voivodeship, located in the south of Poland, occurred mainly in spring, while periods of excessive short-term rainfall in summer. The period of excessive annual precipitation occurred between 2007 and 2010, followed by a long period of dry or very dry years that lasted until 2016. During the period of excess annual precipitation, the leachate from the waste dump caused a decrease in the pH of groundwater from ca. 5 to 2.50, while an increase in electrolytic conductivity (EC) and the concentration of sulphates and zinc from ca. 1300 ms/cm, 100 mg/L, 5 mg/L to 5100 ms/cm, 3890 mg/L, 18.5 mg/L, respectively

The impact of precipitation on state of the slopes surface and thermal activity of the mine waste dump - preliminary study

Precipitation, especially with a high intensity, affects the condition of mining waste dumps. The article presents the results of research aimed at determining the impact of rain on water erosion on the slopes of a coal waste dump and its thermal state. Preliminary tests of the condition of two slopes of the coal waste dump in Libiąż (Poland) undertaken in the frameworks of the TEXMIN project was carried out using modern geodesy techniques (low-ceiling photogrammetry and TLS terrestrial laser scanning). The current geometry of the slope surface was faithfully reproduced in the form of a cloud of points with known coordinates x, y, z. The thermal state within the analyzed slopes of the dump was also assessed. Based on thermography studies and measurements of temperature and gas concentrations inside the object, two zones of thermal activity were located on one of the examined slopes. The test results constitute the initial state against which the results of further tests will be compared. This will allow to determine the influence of precipitation on the amount of water erosion and the thermal state of the dump in a specific time period

The impact of precipitation on the groundwater of coal waste dump

The aim of the study was to assess the effect of climate change, mainly higher and lower precipitation, on the intensity of the impact of a coal waste dump on groundwater. The analysis used meteorological data for the Katowice region in 2002-2020 as well as data on the height of the groundwater table in the vicinity of the coal waste dump, and data on physicochemical parameters and chemical composition of groundwater in 2004-2020. Based on the analyses, it was found that the periods of drought in the Silesian Voivodeship, located in the south of Poland, occurred mainly in spring, while periods of excessive short-term rainfall in summer. The period of excessive annual precipitation occurred between 2007 and 2010, followed by a long period of dry or very dry years that lasted until 2016. During the period of excess annual precipitation, the leachate from the waste dump caused a decrease in the pH of groundwater from ca. 5 to 2.50, while an increase in electrolytic conductivity (EC) and the concentration of sulphates and zinc from ca. 1300 ms/cm, 100 mg/L, 5 mg/L to 5100 ms/cm, 3890 mg/L, 18.5 mg/L, respectively

The impact of precipitation on state of the slopes surface and thermal activity of the mine waste dump – preliminary study

Precipitation, especially with a high intensity, affects the condition of mining waste dumps. The article presents the results of research aimed at determining the impact of rain on water erosion on the slopes of a coal waste dump and its thermal state. Preliminary tests of the condition of two slopes of the coal waste dump in Libią_z (Poland) undertaken in the frameworks of the TEXMIN project was carried out using modern geodesy techniques (low-ceiling photogrammetry and TLS terrestrial laser scanning). The current geometry of the slope surface was faithfully reproduced in the form of a cloud of points with known coordinates x, y, z. The thermal state within the analyzed slopes of the dump was also assessed. Based on thermography studies and measurements of temperature and gas concentrations inside the object, two zones of thermal activity were located on one of the examined slopes. The test results constitute the initial state against which the results of further tests will be compared. This will allow to determine the influence of precipitation on the amount of water erosion and the thermal state of the dump in a specific time period.This project has received funding from the Research Fund for Coal and Steelunder grant agreement No. 847250 TEXMIN The Impact of Extreme Weather Events on Mining Operations. Scientific work published as part of an international project co-funded by the Ministry of Science and Higher Education entitled "PMW" in the years 2019-2022 contract No. 5009/FBWiS/2019/2

Elektrofizjologia inwazyjna Przezskórna ablacja nawrotnego pooperacyjnego częstoskurczu przedsionkowego u chłopca po korekcji złożonej wrodzonej wady serca – skorygowanym przełożeniu głównych tętnic z cechami pentalogii Fallota, anomalii Ebsteina i zespołem WPW

In a 14 year old boy with corrected transposition of great arteries after correction of Fallot-like pentalogy, Ebstein-like tricuspid valve anomaly and WPW syndrome that was treated surgically, atrial tachycardia (AT) 260-280ms was diagnosed and ablated. Due to earlier therapy it was impossible to insert catheters through femoral veins, and a subclavian and carotid veins access was used. The arrhythmia was a periincisional right AT. The isthmus was diagnosed with the use of entrainment pacing between a scar near VCI and the surgical incision along crista terminalis. Application in that region terminated AT and it was not inducible after the procedure