GPS Data Analysis for Understanding Urban Goods Movement

AbstractThis paper aims to investigate the contribution of GPS survey techniques for urban goods movement characterization and diagnosis, more precisely the implementation and application issues related to the introduction of real-time data transmission procedures and phone tools with integrated GPS devices. We propose a GPS-based data collection method for urban freight route characterization using a Smartphone application. After testing and calibrating the data processing tool, we analyze the main results on a baseline of about 900 rounds, with the R software. This analysis allows us to define the characteristics of the overall routes as well as the environmental impacts linked with the categories of roads: urban highways, main roads and residential streets. Moreover, the study shows that the environmental behavior of the driver is connected with the main activity of the carriers. The complementarity between GPS and traditional urban freight surveys is finally discussed

Estat de l'art de la millora genètica de la vinya

In this work, a review is conducted on the state of the art of genetic improvement in grapevine (Vitis vinifera), a species belonging to the Vitaceae family. It is part of the botanical genus Vitis, which includes about 60 species, with the most well-known and widely cultivated being Vitis vinifera. Despite the arrival of phylloxera (Dactylosphaera vitifoliae) and the importation of North American pathogens, which caused high mortality among European vines, the different wild species related to Europe have not been lost, although they were at risk of extinction during the process. Currently, about 10,000 cultivated grapevine varieties are known, many of which are preserved in germplasm banks worldwide. There are many studies showing that, despite many names designating varieties, it is estimated that almost 50% of the estimated diversity consists of duplicates. The most widely cultivated wine grape varieties globally are Cabernet Sauvignon, Sultanina, Merlot, and Tempranillo. These varieties are traditional, meaning they have not been obtained through directed genetic improvement programs. Although in the world of grapevines, only varieties obtained through traditional methods are accepted due to restrictive legislation in the wine sector, there is currently a lot of research on the use of other techniques such as mutagenesis, somaclonal variation, somatic embryogenesis, genetic engineering, or CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). The latter seems to be the one that will have a more prominent role in the future, as it is the newest and most precise technique for genetically editing "on demand." Primarily, researchers focus on improving resistance to pathogens and the organoleptic characteristics of the grapes. This work provides a summary of all the techniques that have been used to improve grapevines, whether only in experimental studies without transfer to the production sector or in genetic improvement programs to obtain varieties that have ultimately been cultivatedEn aquest treball es fa una revisió sobre l’estat de l’art de la millora genètica en vinya (Vitis vinifera), espècie pertanyent a la família Vitaceae. Forma part del gènere botànic Vitis, que conté unes 60 espècies, essent la més coneguda i més cultivada Vitis vinifera. Tot i l’arribada de la fil·loxera (Dactylosphaera vitifoliae) i la importació de patògens Nord-americans, que van provocar una elevada mortalitat entre les vinyes europees, no s’han perdut les diferents espècies silvestres emparentades a Europa, tot i que en el procés van estar en perill d’extinció. Actualment es coneixen unes 10.000 varietats de vinya cultivades, de les quals moltes es conserven en bancs de germoplasma arreu del món. Hi ha molts estudis que mostren que tot i existir un gran nombre de noms designant varietats, es pot estimar que gairebé el 50% de la diversitat estimada són duplicats. Les varietats vinificables més cultivades a nivell mundial són el Cabernet Sauvignon, la Sultanina, el Merlot i el Tempranillo. Aquestes varietats son tradicionals, es a dir, no han sigut obtingudes a partir de programes de millora genètica dirigits. Tot i que en el món de la vinya només s’accepten varietats obtingudes mitjançant mètodes tradicionals, degut a la restrictiva legislació que existeix en el sector del vi, actualment hi ha molta investigació sobre l’ús d’altres tècniques com la mutagènesis, la variació soma-clonal, l’embriogènesi somàtica, l’enginyeria genètica o el CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). Aquesta última sembla ser la que tindrà un ús més notori en un futur, ja que es la tècnica més nova i amb més precisió per poder editar genèticament ‘a la carta’. Principalment, els investigadors es centren en la millora de la resistència a patògens i la millora del raïm per a que aquest tingui millors característiques organolèptiques. Aquest treball presenta un resum de totes les tècniques que s’han fet servir per millorar la vinya, ja sigui només treball experimental sense transferència al sector productiu o treballs que s’han transformat en programes de millora genètica per obtenir varietats que finalment s’han arribat a cultivar.En este trabajo se realiza una revisión sobre el estado del arte de la mejora genética en la vid (Vitis vinifera), una especie perteneciente a la familia Vitaceae. Forma parte del género botánico Vitis, que incluye unas 60 especies, siendo la más conocida y cultivada la Vitis vinifera. A pesar de la llegada de la filoxera (Dactylosphaera vitifoliae) y la importación de patógenos de América del Norte, lo que causó una alta mortalidad en las vides europeas, no se han perdido las diferentes especies silvestres relacionadas con Europa, aunque durante el proceso estuvieron en peligro de extinción. Actualmente se conocen unas 10,000 variedades de vid cultivadas, muchas de las cuales se conservan en bancos de germoplasma en todo el mundo. Hay muchos estudios que muestran que, a pesar de existir un gran número de nombres que designan variedades, se estima que casi el 50% de la diversidad estimada son duplicados. Las variedades viníferas más cultivadas a nivel mundial son el Cabernet Sauvignon, la Sultanina, el Merlot y el Tempranillo. Estas variedades son tradicionales, es decir, no han sido obtenidas a partir de programas de mejora genética dirigidos. Aunque en el mundo de la vid solo se aceptan variedades obtenidas mediante métodos tradicionales debido a la restrictiva legislación en el sector del vino, actualmente existe mucha investigación sobre el uso de otras técnicas como la mutagénesis, la variación somaclonal, la embriogénesis somática, la ingeniería genética o el CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). Esta última parece ser la que tendrá un uso más destacado en el futuro, ya que es la técnica más nueva y precisa para la edición genética "a medida". Principalmente, los investigadores se centran en mejorar la resistencia a patógenos y las características organolépticas del racimo. Este trabajo presenta un resumen de todas las técnicas utilizadas para mejorar la vid, ya sea solo en trabajos experimentales sin transferencia al sector productivo o en programas de mejora genética para obtener variedades que finalmente se han llegado a cultivar.Objectius de Desenvolupament Sostenible::15 - Vida d'Ecosistemes TerrestresObjectius de Desenvolupament Sostenible::13 - Acció per al Clim

Petits théâtres en quête d’auteures. Daniel Canty traducteur et metteur en livre

En 2013, dans un petit livre bleu publié aux éditions du Noroît à Montréal, l’écrivain-traducteur-metteur en livre Daniel Canty nous offre d’entrer (en français) dans les Petits théâtres (Teatriños) ou Aturuxos calados d’une poète, essayiste, traductrice, Erín Moure, dont l’œuvre s’écrit à la rencontre des langues (anglaise, galicienne) et des signatures en ouvrant ses pages à d’autres voix, telle celle d’une certaine Elisa Sampedrín. La traduction de Daniel Canty se glisse dans les ondoiements du texte pour prolonger et même amplifier le courant de potentialisation qui anime l’œuvre d’Erín Moure : les langues se diffractent et se mêlent, les identités auctoriales se multiplient, entraînant à leur suite une remise en jeu du geste de la traduction, du passage d’une œuvre à une autre — et d’une personne à une autre. Se dessine dans cette œuvre aux multiples visages, où chacun se désapproprie, une éthique des altérités plurielles.Edited in 2013 by the éditions du Noroît (Montréal), Petits théâtres (Teatriños) ou Aturuxos calados offers access (in French) to a book written (in English and Galician) by the poet, essayist and translator Erín Moure, whose voice intertwines with others, namely with Elisa Sampedrín’s. Translator-writer-metteur en livre Daniel Canty expands this potentiation flow: tongues and languages diffract and mingle, authorships demultiply, entangling the questioning of the act of translating as well as the becoming of each person as another. Rich of many faces, this work deploys an ethics of plural alterities

Rapid and accurate quantification of isomiRs by RT-qPCR

Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we describe a simple reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR)-based assay for quantification of the miR-100-5p_iso_3p:−2 variant. We chose miR-100-5p because the canonical sequence was underrepresented in our evaluation of human plasma. The quantification of miR-100-5p_iso_3 p:−2 from 57 plasma samples demonstrated high concordance between high-throughput RNA sequencing and RT-qPCR results (r = 0.55, p < 0.0001). Of note, we could not detect or quantify miR-100-5p in our plasma samples using a commercial TaqMan canonical miR-100-5p RT-qPCR kit. With these 57 samples, we also adapted this assay to specifically quantify the canonical sequences of miR-122-5p and miR-192-5p. Similar to the results obtained with miR-100-5p_iso_3p:−2, RT-qPCR results for miR-122-5p and miR-192-5p highly correlated with high-throughput RNA sequencing data (miR-122-5p: r = 0.44, p = 0.0005; miR-192-5p: r = 0.72, p < 0.0001). The assay described here can be easily adapted to many different identified isomiRs. Because of the high specificity of isomiRs, their reliable RT-qPCR-based quantification could provide greater resolution and higher accuracy than using canonical sequences

GOMAYTEX [Material gráfico]

Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201

Draft genome sequences of Mycobacterium setense type strain DSM-45070 and the nonpathogenic strain Manresensis, isolated from the bank of the Cardener river in Manresa, Catalonia, Spain

This study was funded by the Spanish Government, Instituto de Salud Carlos III (ISCIII), through the CIBER CRP-TB and the FIS 011/01702 grants to PJC, and by Manresana de Micobacteriologia, s.l. Additional support came from IMPPC core funding from the Generalitat de Catalunya to LS. JV, GR, SS, were funded by grant ADE10/00026 from ISCIII. JV was also co-funded by ISIS contract II11/00014 from ISCIII. IC is supported by the Spanish Ministerio de Economia y Competitividad (MINECO) through a Ramón y Cajal contract RYC-2012-10627 and grant SAF2013-43521-R. E.J. was funded by Instituto de Salud Carlos III-PI1001438 and the European Regional Development Fund (FEDER), and CV by Miguel Servet Contract CP13/00174.We present here the draft genome sequences of two Mycobacterium setense strains. One of them corresponds to the M. setense type strain DSM-45070, originally isolated from a patient with a posttraumatic chronic skin abscess. The other one corresponds to the nonpathogenic M. setense strain Manresensis, isolated from the Cardener River crossing Manresa, Catalonia, Spain. A comparative genomic analysis shows a smaller genome size and fewer genes in M. setense strain Manresensis relative to those of the type strain, and it shows the genome segments unique to each strain

Draft genome sequences of Mycobacterium setense type strain DSM-45070 and the nonpathogenic strain Manresensis, isolated from the bank of the Cardener River in Manresa, Catalonia, Spain

We present here the draft genome sequences of two Mycobacterium setense strains. One of them corresponds to the M. setense type strain DSM-45070, originally isolated from a patient with a posttraumatic chronic skin abscess. The other one corresponds to the nonpathogenic M. setense strain Manresensis, isolated from the Cardener River crossing Manresa, Catalonia, Spain. A comparative genomic analysis shows a smaller genome size and fewer genes in M. setense strain Manresensis relative to those of the type strain, and it shows the genome segments unique to each strain

Simulateur de Production Solaire : un outil pédagogique innovant dédié à l’enseignement des principes fondamentaux de l’énergie photovoltaïque

Cet article décrit une nouvelle approche pédagogique pour l’enseignement des connaissances de base de l'énergie solaire et des applications photovoltaïques. Cette méthode d'enseignement expérimentale et interactive permet aux étudiants et aux concepteurs de systèmes photovoltaïques de manipuler un simulateur de production solaire. Cet outil de simulation reproduit à échelle réduite la trajectoire du soleil quelle que soit la période de l’année. La production d'énergie est ensuite calculée pour différentes inclinaisons et orientations d'un capteur solaire. Le simulateur offre aux étudiants l'occasion d'acquérir d’une façon ludique les fondamentaux de l’énergie photovoltaïque. Plusieurs exemples de travaux pratiques sont détaillés afin de donner une vue d'ensemble des exercices d'apprentissage qui pourraient être faits par les étudiants pour découvrir les multiples applications du photovoltaïque. À titre d’exemple, de nombreux cas d’études peuvent être traités tels que la mise en oeuvre des calculs de position du soleil, la caractérisation électrique des cellules solaires avec différentes technologies ou encore la programmation de suiveurs du point de puissance maximale

Curcumin mediates oxaliplatin-acquired resistance reversion in colorectal cancer cell lines through modulation of CXC-Chemokine/NF-κB signalling pathway

This study was funded by the ISCIII grant, project n° PI1202228 and Departament d'Innovació, Universitats i Empresa, Generalitat de Catalunya. SGR-PREDIVHICO. This work was done under the framework of the doctorate in Medicine from the Universitat Autònoma de Barcelona. We thank Dr. Lucía Sanjurjo (Innate Immunity Group, IGTP, Badalona, Spain) for her technical assistance and support and Dr. Verónica Guirao (Biobank research support unit, IGTP, Badalona, Spain) for her comments and editorial assistance.Resistance to oxaliplatin (OXA) is a complex process affecting the outcomes of metastatic colorectal cancer (CRC) patients treated with this drug. De-regulation of the NF-κB signalling pathway has been proposed as an important mechanism involved in this phenomenon. Here, we show that NF-κB was hyperactivated in in vitro models of OXA-acquired resistance but was attenuated by the addition of Curcumin, a non-toxic NF-κB inhibitor. The concomitant combination of Curcumin + OXA was more effective and synergistic in cell lines with acquired resistance to OXA, leading to the reversion of their resistant phenotype, through the inhibition of the NF-κB signalling cascade. Transcriptomic profiling revealed the up-regulation of three NF-κB-regulated CXC-chemokines, CXCL8, CXCL1 and CXCL2, in the resistant cells that were more efficiently down-regulated after OXA + Curcumin treatment as compared to the sensitive cells. Moreover, CXCL8 and CXCL1 gene silencing made resistant cells more sensitive to OXA through the inhibition of the Akt/NF-κB pathway. High expression of CXCL1 in FFPE samples from explant cultures of CRC patients-derived liver metastases was associated with response to OXA + Curcumin. In conclusion, we suggest that combination of OXA + Curcumin could be an effective treatment, for which CXCL1 could be used as a predictive marker, in CRC patients

Curcumin mediates oxaliplatin-acquired resistance reversion in colorectal cancer cell lines through modulation of CXC-Chemokine/NF-κB signalling pathway

Resistance to oxaliplatin (OXA) is a complex process affecting the outcomes of metastatic colorectal cancer (CRC) patients treated with this drug. De-regulation of the NF-kappa B signalling pathway has been proposed as an important mechanism involved in this phenomenon. Here, we show that NF-kappa B was hyperactivated in in vitro models of OXA-acquired resistance but was attenuated by the addition of Curcumin, a non-toxic NF-kappa B inhibitor. The concomitant combination of Curcumin + OXA was more effective and synergistic in cell lines with acquired resistance to OXA, leading to the reversion of their resistant phenotype, through the inhibition of the NF-kappa B signalling cascade. Transcriptomic profiling revealed the up-regulation of three NF-kappa B-regulated CXC-chemokines, CXCL8, CXCL1 and CXCL2, in the resistant cells that were more efficiently down-regulated after OXA + Curcumin treatment as compared to the sensitive cells. Moreover, CXCL8 and CXCL1 gene silencing made resistant cells more sensitive to OXA through the inhibition of the Akt/NF-kappa B pathway. High expression of CXCL1 in FFPE samples from explant cultures of CRC patients-derived liver metastases was associated with response to OXA + Curcumin. In conclusion, we suggest that combination of OXA + Curcumin could be an effective treatment, for which CXCL1 could be used as a predictive marker, in CRC patients