SYSTEM, METHOD, AND COMPUTER PROGRAM PRODUCT FOR AUTOMATICALLY SCRAPING CATEGORICAL DATA FROM A PLURALITY OF WEBSITES

Systems, methods, and computer program products are provided for automatically scraping categorical data from a plurality of websites. These include: determining a product category; identifying a first website including data associated with the product category; automatically scraping the first website to compile first product data associated with the product category; generating a plurality of web queries based on the compiled first product data; executing the plurality of web queries to identify a plurality of websites; automatically scraping at least a portion of the plurality of websites to compile supplier data associated with suppliers in the product category; and storing at least a portion of the compiled supplier data in a database

Highly efficient methods for the one-pot synthesis of b-substituted enones

A mild and practically-convenient one-pot procedure for the direct b-substitution of enones has been developed using a conjugate addition–oxidation strategy with a full range of copper-based reagents and N-tert-butylphenylsulfinimidoyl chloride; alkyl- and aryl-substituted enones are delivered in good to excellent yields

Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.

S-palmitoylation (S-acylation) is an emerging dynamic post-translational modification of cysteine residues within proteins.Current assays for protein S-palmitoylation involve either in vivo labelling or chemical cleavage of S-palmitoyl groupsto reveal a free cysteine sulfhydryl that can be subsequently labelled with an affinity handle (acyl-exchange). Assays for protein S-palmitoylation using acylexchange chemistry therefore require blocking of non-S-palmitoylated cysteines, typically using Nethylmaleimide, to prevent non-specific detection. This in turn necessitates multiple precipitation based clean-up steps to remove reagents between stages, often leading to variable sample loss, reduced signal or protein aggregation. These combine to reduce the sensitivity, reliability and accuracy of these assays and also requires a substantial amount of time to perform. By substituting these precipitation steps with chemical scavenging of N-ethylmaleimide by 2,3-dimethyl-1,3-butadiene in an aqueous Diels-Alder 4+2 cyclo-addition reaction it is possible to greatly improve sensitivity and accuracy while reducing hands-on and overall time required for assays

Intestinal Parasites in an Ottoman Period Latrine from Acre (Israel) Dating to the Early 1800s CE.

The aim of this study is to determine the species of parasites that affected the inhabitants of the city of Acre on the coast of the eastern Mediterranean during the Ottoman Period. This is the first archaeological study of parasites in the Ottoman Empire. We analysed sediment from a latrine dating to the early 1800s for the presence of helminth eggs and protozoan parasites which caused dysentery. The samples were examined using light microscopy and enzyme-linked immunosorbent assay (ELISA) kits. We found evidence for roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), fish tapeworm (Dibothriocephalus sp.), Taenia tapeworm (Taenia sp.), lancet liver fluke (Dicrocoelium dendriticum), and the protozoa Giardia duodenalis and Entamoeba histolytica. The parasite taxa recovered demonstrate the breadth of species present in this coastal city. We consider the effect of Ottoman Period diet, culture, trade and sanitation upon risk of parasitism in this community living 200 years ago

Un support IDM pour l'architecture d'entreprise dans un contexte industriel : l'exemple du framework TEAP

National audienceL'application de l'Ingénierie Dirigée par les Modèles (IDM) est souvent réservée aux processus de génie logiciel (par exemple, spécification, génération de code, maintenance, rétro-ingénierie, évolution). Cependant, elle peut aussi être bénéfique pour des initiatives davantage orientées métier ou liées à la prise de décisions stratégiques, telles que l'Architecture d'Entreprise (AE). L'AE est le processus de traduction de la vision métier/stratégie d'une entreprise en un changement effectif, via la description de son état présent et futur (par exemple, concernant son système d'information). Même si différentes approches ont déjà proposé plusieurs sortes de méthodes et d'outillages pour l'AE dans une entreprise, il n'existe pas véritablement à l'heure actuelle de framework IDM intégré combinant fédération de données d'AE, adaptabilité du standard de représentation et support pour des points de vue multiples. Le présent article rapporte notre expérience en cours de construction du framework IDM TEAP (basé sur le standard TOGAF et l'outillage SmartEA) visant notamment à traiter ces trois défis dans un contexte industriel d'AE

In-vitro and in-vivo evidence for uncoupling of BCR internalization and signaling in chronic lymphocytic leukemia

B-cell receptor activation, occurring within lymph nodes, plays a key role in the pathogenesis of chronic lymphocytic leukemia and is linked to prognosis. As well as activation of downstream signaling, receptor ligation triggers internalization, transit to acidified endosomes and degradation of ligand-receptor complexes. In the present study we investigated the relationship between these two processes in normal and leukemic B-cells. We found that leukemic B-cells, particularly anergic cases lacking the capacity to initiate downstream signaling, internalize and accumulate ligand in acidified endosomes more efficiently than normal B-cells. Furthermore, ligation of either surface CD79B, a Bcell receptor component required for downstream signaling, or surface IgM by cognate agonistic antibody, showed that the two molecules internalize independently of each other in leukemic but not normal B-cells. Since association with surface CD79B is required for surface retention of IgM, this suggests that uncoupling of B-cell receptor internalization from signaling may be due to dissociation of these two molecules in leukemic cells. Comparison of lymph node with peripheral blood cells from chronic lymphocytic leukemia patients showed that, despite recent B-cell receptor activation, lymph node B-cells expressed higher levels of surface IgM. This surprising finding suggests that the B-cell receptors of lymph node and peripheral blood derived leukemic cells might be functionally distinct. Finally, long-term therapy with the Bruton’s tyrosine kinase inhibitors ibrutinib or acalabrutinib resulted in a switch to an anergic pattern of B-cell receptor function with reduced signaling capacity, surface IgM expression and more efficient internalization

ModelExtractor: an Automatic Parametric Model Extractor

International audienceBuilding on previous experiments of reverse engineering with the Squeak language, we have designed a new prototype on the DotNet platform that should be able to exploit the full capabilities of the Visual Basic 9.0 language, including the facilities offered for dynamic typing. The objective of this work is to prove that we are able to implement an automatic model mining facility when the system consists of a legacy with good reflectivity capabilities. The initial work presented in this paper comforts the previous results obtained for Squeak and constitute the basis of a reverse engineering development toolkit that is intended to be extended in the coming months

Capturing Saddam Hussein: How the full story got away, and what conflict journalism can learn from it

The capture of Saddam Hussein in December 2003 was reported with a sense of triumph which must have greatly satisfied the United States forces occupying Iraq. This was the victory they had been looking for; the seminal moment which signalled that the invasion had been a success. But the reporting of that event was also a missed opportunity: an example of incomplete story telling. In this article, I use my personal experience of reporting on the event for the BBC as a starting point to examine what it, and the way it was covered, tell us about the omissions which are frequently a feature of conflict reporting. The article argues that the way in which reporters had to work in Iraq then meant that they did not convey all of the event’s wider implications, and suggests how that might be improved

Genetic algorithm with logistic regression for prediction of progression to Alzheimer\u27s disease

Assessment of risk and early diagnosis of Alzheimer\u27s disease (AD) is a key to its prevention or slowing the progression of the disease. Previous research on risk factors for AD typically utilizes statistical comparison tests or stepwise selection with regression models. Outcomes of these methods tend to emphasize single risk factors rather than a combination of risk factors. However, a combination of factors, rather than any one alone, is likely to affect disease development. Genetic algorithms (GA) can be useful and efficient for searching a combination of variables for the best achievement (eg. accuracy of diagnosis), especially when the search space is large, complex or poorly understood, as in the case in prediction of AD development. This study showed the potential of GA application in the neural science area. It demonstrated that the combination of a small set of variables is superior in performance than the use of all the single significant variables in the model for prediction of progression of disease. Variables more frequently selected by GA might be more important as part of the algorithm for prediction of disease development