7 research outputs found
Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers
Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major outer membrane protein (MOMP) of C. jejuni NCTC11168 binds to the Lewis b (Leb) antigen on the gastrointestinal epithelia of host tissues and this interaction can be competitively inhibited by ferric quinate (QPLEX), a ferric chelate structurally similar to bacterial siderophores. We provide evidence that QPLEX competitively inhibits the MOMP-Leb interaction. Furthermore, we demonstrate that QPLEX can be used as a feed additive in broiler farming to significantly reduce C. jejuni colonization. Our results indicate that QPLEX can be a viable alternative to the preventative use of antibiotics in broiler farming to combat C. jejuni infections
ΠΡΠΎΡΡΠ°ΡΠΈΡΠ½Ρ ΠΊΠΎΠ½ΠΊΡΠ΅ΠΌΠ΅Π½ΡΠΈ Π·ΡΠΌΠΎΠ²Π»ΡΡΡΡ ΠΎΡΡΠ΅ΠΎΠ±Π»Π°ΡΡΠΈΡΠ½ΠΈΠΉ ΡΠΌΡΠ½ΠΎΡΠ΅Π½ΠΎΡΠΈΠΏ ΡΠ°ΠΊΡ ΠΏΠ΅ΡΠ΅Π΄ΠΌΡΡ ΡΡΠΎΠ²ΠΎΡ Π·Π°Π»ΠΎΠ·ΠΈ
Β Prostate cancer (PGC) is leading the way in the structure of cancer morbidity around the world. The presence of prostatic concretions (PC) and skeletal metastases significantly reduces the patientsβ life quality and worsens the prognosis of the disease.Aim. The aim of study was to define the impact of PC on the development of prostate cancer bone metastases.Materials and methods. 30 samples of PGC with PC and 30 PGC tissue samples without PC were analyzed by histology (hematoxylin-eosin staining), histochemistry (von Kossa and Alizarin red staining), immunohistochemistry (OSN, Col I, Col II, p53, Bax, Casp3, MPO, CD68). PC were analyzed by SEM-EDS and XRD with different temperature of heat pretreatment.Results. All PC samples had the calcium hydroxyapatite origin with a slight deviation from the stoichiometric composition and admixture of extraneous elements. The presence of zinc oxide and iron-containing calcium phosphate in the structure of PC was found by XRD. We revealed significantly higher expression of ΠΠ°Ρ
, Π‘Π°sp3, MPO and CD68 in PGC tissue with PC (P < 0.001). We suggest that the combination of these factors predetermines the development of a specific phenotype of cancer cells which is manifested by the significantly higher OSN and Col I expression in PGC with PC (P < 0.001). It contributes to recognizing of bone tissue as an optimal environment for the growth and development of PGC metastases by cancer cells.Conclusions. The presence of PC in the PGC tissue promotes the development of a specific osteoblastic immunophenotype of cancer cells. It can determine the tropism of PGC metastases to bone tissue.Β Β Π Π°ΠΊ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ (Π ΠΠ) Π·Π°Π½ΠΈΠΌΠ°Π΅Ρ Π²Π΅Π΄ΡΡΠΈΠ΅ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΠΈ ΠΈ ΡΠΌΠ΅ΡΡΠ½ΠΎΡΡΠΈ ΠΌΡΠΆΡΠΈΠ½ ΠΏΠΎ Π²ΡΠ΅ΠΌΡ ΠΌΠΈΡΡ. ΠΠ°Π»ΠΈΡΠΈΠ΅ ΠΏΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠΎΠ½ΠΊΡΠ΅ΠΌΠ΅Π½ΡΠΎΠ² (ΠΠ) ΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠΉ ΡΠΊΠ΅Π»Π΅ΡΠ° Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΈ ΡΡ
ΡΠ΄ΡΠ°Π΅Ρ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π°Π»ΠΈΡΠΈΡ ΠΠ Π½Π° ΠΏΡΠΎΡΠ΅ΡΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΠΎΡΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² Π ΠΠ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. 30 ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΠΊΠ°Π½ΠΈ Π ΠΠ Ρ ΠΠ ΠΈ 30 ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π ΠΠ Π±Π΅Π· ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π±ΠΈΠΎΠΌΠΈΠ½Π΅ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
(ΠΎΠΊΡΠ°ΡΠΊΠ° Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ), Π³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
(ΠΎΠΊΡΠ°ΡΠΊΠ° Π°Π»ΠΈΠ·Π°ΡΠΈΠ½ΠΎΠ²ΡΠΌ ΠΊΡΠ°ΡΠ½ΡΠΌ ΠΈ ΠΏΠΎ ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠΎΠ½ ΠΠΎΡΡΠ°) ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
(Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°Π½ΡΠΈΡΠ΅Π» ΠΏΡΠΎΡΠΈΠ² ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² OSN, Col I, Col II, p53, Bax, Casp3, MPO, CD68) ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ². ΠΡΠ΅ ΠΠ ΠΏΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ SEM-EDS ΠΈ XRD Ρ ΡΠ°Π·Π½ΡΠΌΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΡΠΌΠΈ ΡΠ΅ΠΆΠΈΠΌΠ°ΠΌΠΈ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΠ ΡΠΎΡΡΠΎΡΠ»ΠΈ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Ρ ΠΊΠ°Π»ΡΡΠΈΡ Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ°ΠΏΠ°ΡΠΈΡΠ° Ρ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΠΎΡΠΊΠ»ΠΎΠ½Π΅Π½ΠΈΡΠΌΠΈ Π² ΡΡΠ΅Ρ
ΠΈΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠΎΡΡΠ°Π²Π΅ ΠΈ ΠΏΡΠΈΠΌΠ΅ΡΡΠΌΠΈ ΠΏΠΎΡΡΠΎΡΠΎΠ½Π½ΠΈΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ². Π‘ ΠΏΠΎΠΌΠΎΡΡ XRD Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΎΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° ΠΏΡΠΈ 900 ΒΊΠ‘ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΎΠΊΡΠΈΠ΄Π° ΡΠΈΠ½ΠΊΠ° ΠΈ ΠΆΠ΅Π»Π΅Π·ΠΎΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠ΅Π³ΠΎ ΠΊΠ°Π»ΡΡΠΈΡ ΡΠΎΡΡΠ°ΡΠ° Π² Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠΎΡΡΠ°Π²Π΅ ΠΠ. ΠΡΠΌΠ΅ΡΠ΅Π½Ρ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠ΅ ΡΡΠΎΠ²Π½ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΠ°Ρ
, Π‘Π°sp3, MPO ΠΈ Π‘D68 Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π ΠΠ Ρ Π½Π°Π»ΠΈΡΠΈΠ΅ΠΌ ΠΠ (p < 0,001). Π‘ΡΠΈΡΠ°Π΅ΠΌ, ΡΡΠΎ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ ΡΠΊΠ°Π·Π°Π½Π½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΡΠΎΠ·Π΄Π°Π΅Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ Π΄Π»Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΡΠΎ ΠΏΡΠΎΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ OSN ΠΈ Col I Π² Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π ΠΠ (p < 0,001). ΠΡΠΎ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ°ΡΠΏΠΎΠ·Π½Π°Π²Π°Π½ΠΈΡ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠΎΠΊΡΡΠΆΠ΅Π½ΠΈΡ Π΄Π»Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈ ΡΠΎΡΡΠ° ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² Π ΠΠ.ΠΡΠ²ΠΎΠ΄Ρ. ΠΠ°Π»ΠΈΡΠΈΠ΅ ΠΠ Π² ΡΠΊΠ°Π½ΠΈ Π ΠΠ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΡΠ΅ΠΎΠ±Π»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΡΠ»Π°Π²Π»ΠΈΠ²Π°ΡΡ ΡΡΠΎΠΏΠΈΠ·ΠΌ Π ΠΠ ΠΊ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΠΎΡΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ².Β Β Π Π°ΠΊ ΠΏΠ΅ΡΠ΅Π΄ΠΌΡΡ
ΡΡΠΎΠ²ΠΎΡ Π·Π°Π»ΠΎΠ·ΠΈ (Π ΠΠ) ΠΏΠΎΡΡΠ΄Π°Ρ ΠΏΡΠΎΠ²ΡΠ΄Π½Ρ ΠΏΠΎΠ·ΠΈΡΡΡ Ρ ΡΡΡΡΠΊΡΡΡΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΡ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½ΠΎΡΡΡ ΡΠ° ΡΠΌΠ΅ΡΡΠ½ΠΎΡΡΡ ΡΠΎΠ»ΠΎΠ²ΡΠΊΡΠ² Ρ Π²ΡΡΠΎΠΌΡ ΡΠ²ΡΡΡ. ΠΠ°ΡΠ²Π½ΡΡΡΡ ΠΏΡΠΎΡΡΠ°ΡΠΈΡΠ½ΠΈΡ
ΠΊΠΎΠ½ΠΊΡΠ΅ΠΌΠ΅Π½ΡΡΠ² (ΠΠ) Ρ ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΆΠ΅Π½Π½Ρ ΡΠΊΠ΅Π»Π΅ΡΠ° Π·Π½Π°ΡΠ½ΠΎ Π·Π½ΠΈΠΆΡΡ ΡΠΊΡΡΡΡ ΠΆΠΈΡΡΡ ΠΏΠ°ΡΡΡΠ½ΡΡΠ² Ρ ΠΏΠΎΠ³ΡΡΡΡΡ ΠΏΠ΅ΡΠ΅Π±ΡΠ³ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Π½Ρ.ΠΠ΅ΡΠ° ΡΠΎΠ±ΠΎΡΠΈ β Π²ΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΠΌΠΎΠΆΠ»ΠΈΠ²ΠΎΠ³ΠΎ Π²ΠΏΠ»ΠΈΠ²Ρ Π½Π°ΡΠ²Π½ΠΎΡΡΡ ΠΠ Π½Π° ΠΏΡΠΎΡΠ΅ΡΠΈ ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΠΊΡΡΡΠΊΠΎΠ²ΠΈΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΡΠ² Π ΠΠ.ΠΠ°ΡΠ΅ΡΡΠ°Π»ΠΈ ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈ. 30 Π·ΡΠ°Π·ΠΊΡΠ² ΡΠΊΠ°Π½ΠΈΠ½ΠΈ Π ΠΠ ΡΠ· ΠΠ Ρ 30 Π·ΡΠ°Π·ΠΊΡΠ² Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ½ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ Π ΠΠ Π±Π΅Π· ΠΎΠ·Π½Π°ΠΊ Π±ΡΠΎΠΌΡΠ½Π΅ΡΠ°Π»ΡΠ·Π°ΡΡΡ Π΄ΠΎΡΠ»ΡΠ΄ΠΈΠ»ΠΈ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ Π³ΡΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
(Π·Π°Π±Π°ΡΠ²Π»Π΅Π½Π½Ρ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΡΠ½ΠΎΠΌ ΡΠ° Π΅ΠΎΠ·ΠΈΠ½ΠΎΠΌ), Π³ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΈΡ
(Π·Π°Π±Π°ΡΠ²Π»Π΅Π½Π½Ρ Π°Π»ΡΠ·Π°ΡΠΈΠ½ΠΎΠ²ΠΈΠΌ ΡΠ΅ΡΠ²ΠΎΠ½ΠΈΠΌ Ρ Π·Π° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΠ½ ΠΠΎΡΡΠ°) ΡΠ° ΡΠΌΡΠ½ΠΎΠ³ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΈΡ
(Π· Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½ΡΠΌ Π°Π½ΡΠΈΡΡΠ» ΠΏΡΠΎΡΠΈ ΠΌΠ°ΡΠΊΠ΅ΡΡΠ² OSN, Col I, Col II, p53, Bax, Casp3, MPO, CD68) ΠΌΠ΅ΡΠΎΠ΄ΡΠ². Π£ΡΡ ΠΠ Π°Π½Π°Π»ΡΠ·ΡΠ²Π°Π»ΠΈ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ SEM-EDS Ρ XRD Π· ΡΡΠ·Π½ΠΈΠΌΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΈΠΌΠΈ ΡΠ΅ΠΆΠΈΠΌΠ°ΠΌΠΈ ΠΎΠ±ΡΠΎΠ±ΠΊΠΈ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ. Π£ΡΡ Π·ΡΠ°Π·ΠΊΠΈ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½ΠΈΡ
ΠΠ ΡΠΊΠ»Π°Π΄Π°Π»ΠΈΡΡ ΠΏΠ΅ΡΠ΅Π²Π°ΠΆΠ½ΠΎ ΡΠ· ΠΊΠ°Π»ΡΡΡΡ Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠ°ΠΏΠ°ΡΠΈΡΡ Π· Π½Π΅Π·Π½Π°ΡΠ½ΠΈΠΌΠΈ Π²ΡΠ΄Ρ
ΠΈΠ»Π΅Π½Π½ΡΠΌΠΈ Ρ ΡΡΠ΅Ρ
ΡΠΎΠΌΠ΅ΡΡΠΈΡΠ½ΠΎΠΌΡ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° Π΄ΠΎΠΌΡΡΠΊΠ°ΠΌΠΈ ΡΡΠΎΡΠΎΠ½Π½ΡΡ
Π΅Π»Π΅ΠΌΠ΅Π½ΡΡΠ². ΠΠ° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ XRD Π· ΠΎΠ±ΡΠΎΠ±ΠΊΠΎΡ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ ΠΏΡΠΈ 900 ΒΊΠ‘ Π²ΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ Π½Π°ΡΠ²Π½ΡΡΡΡ ΠΎΠΊΡΠΈΠ΄Ρ ΡΠΈΠ½ΠΊΡ ΡΠ° Π·Π°Π»ΡΠ·ΠΎΠ²ΠΌΡΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°Π»ΡΡΡΡ ΡΠΎΡΡΠ°ΡΡ Π² Ρ
ΡΠΌΡΡΠ½ΠΎΠΌΡ ΡΠΊΠ»Π°Π΄Ρ ΠΠ. ΠΠΈΠ·Π½Π°ΡΠΈΠ»ΠΈ Π·Π½Π°ΡΠ½ΠΎ Π²ΠΈΡΡ ΡΡΠ²Π½Ρ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ ΠΠ°Ρ
, Π‘Π°sp3, MPO ΡΠ° Π‘D68 Ρ ΠΏΡΡ
Π»ΠΈΠ½Π½ΡΠΉ ΡΠΊΠ°Π½ΠΈΠ½Ρ Π ΠΠ ΡΠ· Π½Π°ΡΠ²Π½ΡΡΡΡ ΠΠ (p < 0,001). ΠΠ²Π°ΠΆΠ°ΡΠΌΠΎ, ΡΠΎ ΠΊΠΎΠΌΠ±ΡΠ½Π°ΡΡΡ ΡΠΈΡ
ΡΠ°ΠΊΡΠΎΡΡΠ² ΡΡΠ²ΠΎΡΡΡ ΡΠΌΠΎΠ²ΠΈ Π΄Π»Ρ ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΡΠΏΠ΅ΡΠΈΡΡΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π½ΠΎΡΠΈΠΏΡ ΠΏΡΡ
Π»ΠΈΠ½Π½ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½, ΡΠΎ ΠΏΡΠΎΡΠ²Π»ΡΡΡΡΡΡ ΠΏΡΠ΄Π²ΠΈΡΠ΅Π½ΠΎΡ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡΡ OSN ΡΠ° Col I Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ½ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΎΡ Π ΠΠ ΡΠ· ΠΠ (p < 0,001). Π¦Π΅ ΡΠΏΡΠΈΡΠΈΠ½ΡΡ ΡΠΎΠ·ΠΏΡΠ·Π½Π°Π²Π°Π½Π½Ρ ΠΊΡΡΡΠΊΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΠΏΡΡ
Π»ΠΈΠ½Π½ΠΈΠΌΠΈ ΠΊΠ»ΡΡΠΈΠ½Π°ΠΌΠΈ ΡΠΊ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΡΠΊΡΠΎΠΎΡΠΎΡΠ΅Π½Π½Ρ Π΄Π»Ρ ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΡΠ° ΡΠΎΡΡΡ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΡΠ² Π ΠΠ.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΠ°ΡΠ²Π½ΡΡΡΡ ΠΠ Ρ ΡΠΊΠ°Π½ΠΈΠ½Ρ Π ΠΠ ΡΡΠΏΡΠΎΠ²ΠΎΠ΄ΠΆΡΡΡΡΡΡ ΡΠΎΠ·Π²ΠΈΡΠΊΠΎΠΌ ΡΠΏΠ΅ΡΠΈΡΡΡΠ½ΠΎΠ³ΠΎ ΠΎΡΡΠ΅ΠΎΠ±Π»Π°ΡΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΠΌΡΠ½ΠΎΡΠ΅Π½ΠΎΡΠΈΠΏΡ ΠΏΡΡ
Π»ΠΈΠ½Π½ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½. Π¦Π΅ ΠΌΠΎΠΆΠ΅ Π·ΡΠΌΠΎΠ²Π»ΡΠ²Π°ΡΠΈ ΡΡΠΎΠΏΡΠ·ΠΌ Π ΠΠ Π΄ΠΎ ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΠΊΡΡΡΠΊΠΎΠ²ΠΈΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΡΠ²
ΠΠΈΠΏΠ°Π΄ΠΎΠΊ ΡΠΈΠ½Ρ ΡΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠ°ΠΊΡ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ Ρ Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ. ΠΠ»ΡΠ½ΡΡΠ½Π΅ ΡΠΏΠΎΡΡΠ΅ΡΠ΅ΠΆΠ΅Π½Π½Ρ
Background. The problems of pre-operative cancer diagnosis of the fallopian tube are associated with a rare occurrence of this disease. More rarely, there is a combination of the fallopian tube cancer and other gynecological tumors. Clinical observation of the fallopian tube cancer and endometrial cancer in postmenopausal woman is presented, which demonstrates the difficulty of the pre-operative diagnostics of these pathologies combination.Case presentation. We described a case of uterine tube cancer in Ukrainian woman (77 years), which had been combined with adenocarcinoma of an endometrium.Conclusion. Primary fallopian tubes carcinoma is a rare disease with an insufficiently studied etiology. The clinical manifestations are not always present in full. Diagnosis in pre-operative period is very hard to establish. Only the qualitative morphological investigation allows establishing the primary lesion of the fallopian tube. Introducing immunohistochemical investigations in practice serves as additional methods for correct diseases diagnostic, which allow more fully characterizing the prognosis and suggesting appropriate treatment of the patients.Β ΠΡΠΎΠ±Π»Π΅ΠΌΡ Π΄ΠΎΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ°ΠΊΠ° ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ ΡΠ²ΡΠ·Π°Π½Ρ Ρ ΡΠ΅Π΄ΠΊΠΎΠΉ Π²ΡΡΡΠ΅ΡΠ°Π΅ΠΌΠΎΡΡΡΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. ΠΡΠ΅ ΡΠ΅ΠΆΠ΅ ΠΎΡΠΌΠ΅ΡΠ°ΡΡ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ ΡΠ°ΠΊΠ° ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ ΠΈ Π΄ΡΡΠ³ΠΈΡ
Π³ΠΈΠ½Π΅ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΎ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ ΡΠ°ΠΊΠ° ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ ΠΈ ΡΠ°ΠΊΠ° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ Ρ ΠΆΠ΅Π½ΡΠΈΠ½Ρ Π² ΠΏΠΎΡΡΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Π΅, ΠΊΠΎΡΠΎΡΠΎΠ΅ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΡΠ΅Ρ ΡΠ»ΠΎΠΆΠ½ΠΎΡΡΠΈ Π΄ΠΎΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Π΄Π°Π½Π½ΡΡ
ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΉ.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠ»ΡΡΠ°Ρ. ΠΠΏΠΈΡΠ°Π»ΠΈ ΡΠ»ΡΡΠ°ΠΉ ΡΠ°ΠΊΠ° ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ Ρ ΡΠΊΡΠ°ΠΈΠ½ΡΠΊΠΎΠΉ ΠΆΠ΅Π½ΡΠΈΠ½Ρ (77 Π»Π΅Ρ), ΠΊΠΎΡΠΎΡΡΠΉ ΡΠΎΡΠ΅ΡΠ°Π»ΡΡ Ρ Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠΎΠΉ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ.ΠΡΠ²ΠΎΠ΄Ρ. ΠΠ΅ΡΠ²ΠΈΡΠ½ΡΠΉ ΡΠ°ΠΊ ΠΌΠ°ΡΠΎΡΠ½ΡΡ
ΡΡΡΠ± β ΡΠ΅Π΄ΠΊΠ°Ρ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡ Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΈΠ·ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΠ΅ΠΉ. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ Π½Π΅ Π²ΡΠ΅Π³Π΄Π° ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡ Π² ΠΏΠΎΠ»Π½ΠΎΠΌ ΠΎΠ±ΡΠ΅ΠΌΠ΅. ΠΠΈΠ°Π³Π½ΠΎΠ· Π² ΠΏΡΠ΅Π΄ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°ΡΡ ΡΠ΅Π΄ΠΊΠΎ. Π’ΠΎΠ»ΡΠΊΠΎ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΡΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ. ΠΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ ΡΠ»ΡΠΆΠ°Ρ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π΄Π»Ρ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Π±ΠΎΠ»Π΅Π΅ ΠΏΠΎΠ»Π½ΠΎ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°ΡΡ ΠΏΡΠΎΠ³Π½ΠΎΠ· ΠΈ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠΈΡΡ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ².Β ΠΡΠΎΠ±Π»Π΅ΠΌΠΈ Π΄ΠΎΠΎΠΏΠ΅ΡΠ°ΡΡΠΉΠ½ΠΎΡ Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ°ΠΊΡ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ ΠΏΠΎΠ²βΡΠ·Π°Π½Ρ Π· ΡΡΠ΄ΠΊΡΡΡΡ Π²ΠΈΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ ΡΡΠΎΠ³ΠΎ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Π½Ρ. Π©Π΅ ΡΡΠ΄ΡΠ΅ ΡΠΏΠΎΡΡΠ΅ΡΡΠ³Π°ΡΡΡ ΠΏΠΎΡΠ΄Π½Π°Π½Π½Ρ ΡΠ°ΠΊΡ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ ΡΠ° ΡΠ½ΡΠΈΡ
Π³ΡΠ½Π΅ΠΊΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
ΠΏΡΡ
Π»ΠΈΠ½. ΠΠ°Π²Π΅Π΄Π΅Π½ΠΎ ΠΊΠ»ΡΠ½ΡΡΠ½Π΅ ΡΠΏΠΎΡΡΠ΅ΡΠ΅ΠΆΠ΅Π½Π½Ρ ΡΠ°ΠΊΡ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ Ρ ΡΠ°ΠΊΡ Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ Ρ ΠΆΡΠ½ΠΊΠΈ Π² ΠΏΠΎΡΡΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Ρ, ΡΠΊΠ΅ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΡΡ ΡΠΊΠ»Π°Π΄Π½ΠΎΡΡΡ Π΄ΠΎΠΎΠΏΠ΅ΡΠ°ΡΡΠΉΠ½ΠΎΡ Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΊΠΎΠΌΠ±ΡΠ½Π°ΡΡΡ ΡΠΈΡ
ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΠΉ.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½Ρ Π²ΠΈΠΏΠ°Π΄ΠΊΡ. ΠΠΏΠΈΡΠ°Π»ΠΈ Π²ΠΈΠΏΠ°Π΄ΠΎΠΊ ΡΠ°ΠΊΡ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ Π² ΡΠΊΡΠ°ΡΠ½ΡΡΠΊΠΎΡ ΠΆΡΠ½ΠΊΠΈ (77 ΡΠΎΠΊΡΠ²), ΡΠΊΠΈΠΉ ΠΏΠΎΡΠ΄Π½ΡΠ²Π°Π²ΡΡ Π· Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠΎΡ Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΠ΅ΡΠ²ΠΈΠ½Π½ΠΈΠΉ ΡΠ°ΠΊ ΠΌΠ°ΡΠΊΠΎΠ²ΠΈΡ
ΡΡΡΠ± Ρ ΡΡΠ΄ΠΊΡΡΠ½ΠΎΡ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡΡ Π· Π½Π΅Π΄ΠΎΡΡΠ°ΡΠ½ΡΠΎ Π²ΠΈΠ²ΡΠ΅Π½ΠΎΡ Π΅ΡΡΠΎΠ»ΠΎΠ³ΡΡΡ. ΠΠ»ΡΠ½ΡΡΠ½Ρ ΠΏΡΠΎΡΠ²ΠΈ Π½Π΅ Π·Π°Π²ΠΆΠ΄ΠΈ Π½Π°ΡΠ²Π½Ρ Π² ΠΏΠΎΠ²Π½ΠΎΠΌΡ ΠΎΠ±ΡΡΠ·Ρ. ΠΡΠ°Π³Π½ΠΎΠ· Ρ ΠΏΠ΅ΡΠ΅Π΄ΠΎΠΏΠ΅ΡΠ°ΡΡΠΉΠ½ΠΎΠΌΡ ΠΏΠ΅ΡΡΠΎΠ΄Ρ Π²ΡΡΠ°Π½ΠΎΠ²Π»ΡΡΡΡ ΡΡΠ΄ΠΊΠΎ. Π’ΡΠ»ΡΠΊΠΈ ΡΠΊΡΡΠ½Π΅ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π΄Π°Ρ ΠΌΠΎΠΆΠ»ΠΈΠ²ΡΡΡΡ Π²ΠΈΠ·Π½Π°ΡΠΈΡΠΈ ΠΏΠ΅ΡΠ²ΠΈΠ½Π½ΡΡΡΡ ΡΡΠ°ΠΆΠ΅Π½Π½Ρ ΠΌΠ°ΡΠΊΠΎΠ²ΠΎΡ ΡΡΡΠ±ΠΈ. ΠΠΏΡΠΎΠ²Π°Π΄ΠΆΠ΅Π½Π½Ρ ΡΠΌΡΠ½ΠΎΠ³ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΈΡ
Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Ρ Ρ ΠΏΡΠ°ΠΊΡΠΈΠΊΡ Ρ Π΄ΠΎΠ΄Π°ΡΠΊΠΎΠ²ΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π»Ρ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΡ Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Ρ, ΡΠΊΡ Π΄Π°ΡΡΡ Π·ΠΌΠΎΠ³Ρ ΡΠΎΡΠ½ΡΡΠ΅ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ²Π°ΡΠΈ ΠΏΡΠΎΠ³Π½ΠΎΠ· Ρ Π·Π°ΠΏΡΠΎΠΏΠΎΠ½ΡΠ²Π°ΡΠΈ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈ Π»ΡΠΊΡΠ²Π°Π½Π½Ρ ΠΏΠ°ΡΡΡΠ½ΡΡΠ².
The case of synchronous fallopian tube and endometrium cancer. Clinical observation
Abstract
Background. The problems of pre-operative cancer diagnosis of the fallopian tube are associated with a rare occurrence of this disease. More rarely, there is a combination of the fallopian tube cancer and other gynecological tumors. Clinical observation of the fallopian tube cancer and endometrial cancer in postmenopausal woman is presented, which demonstrates the difficulty of the pre-operative diagnostics of these pathologies combination.
Case presentation. We described a case of uterine tube cancer in Ukrainian woman (77 years), which had been combined with adenocarcinoma of an endometrium.
Conclusion. Primary fallopian tubes carcinoma is a rare disease with an insufficiently studied etiology. The clinical manifestations are not always present in full. Diagnosis in pre-operative period is very hard to establish. Only the qualitative morphological investigation allows establishing the primary lesion of the fallopian tube. Introducing immunohistochemical investigations in practice serves as additional methods for correct diseases diagnostic, which allow more fully characterizing the prognosis and suggesting appropriate treatment of the patients
Involvement of proinflammatory S100A9/A8 in the atherocalcinosis of aortic valves
According to the results of the Euro-Heart Survey on Vascular Heart Disease the most common pathology is nonrheumatic aortic stenosis, it is also called as calcific aortic valve stenosis (CAVS), as in its pathogenesis the process of biomineralization of valve cusps and ring plays the main role. The aim of the work is the immunohistochemical study of mineralized tissue of aortic heart valves, which are affected by atherocalcinosis. Materials and methods. 30 samples of mineralized aortic valves (I group) and 10 samples of aortic valve without evidence of biomineralization (II group - control) were studied. Immunohistochemical study of expression of collagen (Collagen I), CD68, myeloperoxidase (MPO), calgranulin A (S100A8), calgranulin B (8100A9), caspase 3 (Casp 3) and osteopontin (OPN) was conducted in AV tissue of both groups. Results. In CAV tissues the fibrillar component (collagen I) growths was found, but the quantitative and qualitative compositions of CD68+ circulating inflammatory cells are not significantly different from the control group. CAVs contain much more MPO+-cells (p <0.001) in comparison to the group of AVs without biomineralization. Our data show a significant increase of the S100A9 and OPN expression in the mineralized tissue of AVs (p <0.01). Also, a higher expression level of Casp3 and MPO was found in CAVs (p <0.05). Comparing the first and the second groups of AVs connection between the expression of S100A8 was not determined. Conclusion. High Casp 3 expression confirms the increased level of cell elimination in the CAVs tissue, which is obviously connected with the impact of high local concentrations of S100A9. These facts can contribute to the development of pathological biomineralization of AV. Since osteopontin inhibits the hydroxyapatite formation by binding to the surface of the crystals, its hyperproduction is a counteracting factor against biomineralization in AV tissue
Research of Operating Mode of Rhombic Gravitational Pneumatic Classifier
The paper discusses the technology of obtaining organic and organo-mineral granules of prolonged action. It is found that the granular marketable product must meet certain requirements for particle size. Consequently, the separation unit (classification) in the developed technological scheme plays a very important role in the process of obtaining commodity pellets. The object of research is the process of classification of granular organic fertilizers in the rhombic gravitational pneumatic classifier. The study is aimed at establishing the optimal mode-technological parameters of the Β«rhombicΒ» pneumatic classifier. For this, a physical model of the process of pneumatic classification of dispersed particles (granules) in a rhombic form is studied, which explains the conditions for the separation of a polydisperse mixture into narrower fractions, the formation of a suspended layer of material. As well as a cyclic mechanism for loading and unloading the suspended layer. In addition to ensuring the purity of the product, the apparatus should also have a low hydraulic resistance and low power consumption. For physical modeling, a laboratory bench of a rhombic gravitational pneumatic classifier is used, on which a number of experiments are performed on the selection of the optimal separation mode and product purity. Rational use of the working space and effective ways to influence the flow of material within the same building allows to obtain the required separation parameters. Carrying out the classification process in the Β«rhombicΒ» pneumatic classifier can effectively remove up to 99 % of particles less than 2 mm in size from the granulated product. At the exit of the apparatus, let's obtain a marketable product with a particle size of 2β4 mm in an amount of 99 %, which corresponds to the standard requirements for a qualitative particle size distribution. Such an effective separation in this apparatus is due to its shape (optimal opening angles and closure of the Β«rhombΒ» of the case), which contributes to the rotation of the material flow and leads to an additional reseeding. The absence of contact elements inside the device significantly reduces its hydraulic resistance and reduces energy consumption