Experimental characterization of tensile strength of steel and fibre rovings also under environmental conditioning

The efficiency of the strengthening techniques by externally applied materials can be improved enhancing the debonding strength of the reinforcement from the support by the use of connectors (anchor spikes) consisting of unidirectional bundles of fibres embedded in concrete or masonry by means of organic or inorganic matrices. The use of connectors is suggested in various codes and guidelines of strengthening techniques by composite materials and provisions for their application are given, but currently there are no details for the qualification of the material. In order to investigate anchor spikes made of glass, basalt, aramid, carbon, PBO and steel, a large experimental campaign was carried out at the Materials and Structures Laboratory of the University of Sannio. The tests allowed to evaluate the mechanical characteristics (tensile strength, modulus of elasticity, deformation at the maximum load) of the anchor spikes constituted by only dry fibres, not impregnated, also as a result of environmental conditioning such as freezing and thawing, controlled humidity, alkaline and saline environment

Targeted metabolomic profiling in rat tissues reveals sex differences

Sex differences affect several diseases and are organ-and parameter-specific. In humans and animals, sex differences also influence the metabolism and homeostasis of amino acids and fatty acids, which are linked to the onset of diseases. Thus, the use of targeted metabolite profiles in tissues represents a powerful approach to examine the intermediary metabolism and evidence for any sex differences. To clarify the sex-specific activities of liver, heart and kidney tissues, we used targeted metabolomics, linear discriminant analysis (LDA), principal component analysis (PCA), cluster analysis and linear correlation models to evaluate sex and organ-specific differences in amino acids, free carnitine and acylcarnitine levels in male and female Sprague-Dawley rats. Several intra-sex differences affect tissues, indicating that metabolite profiles in rat hearts, livers and kidneys are organ-dependent. Amino acids and carnitine levels in rat hearts, livers and kidneys are affected by sex: male and female hearts show the greatest sexual dimorphism, both qualitatively and quantitatively. Finally, multivariate analysis confirmed the influence of sex on the metabolomics profiling. Our data demonstrate that the metabolomics approach together with a multivariate approach can capture the dynamics of physiological and pathological states, which are essential for explaining the basis of the sex differences observed in physiological and pathological conditions

Prediction of response to vemurafenib in BRAF V600E mutant cancers based on a network approach

Lung adenocarcinoma is the tumor with the highest number of switch genes (298) compared to its normal tissue, followed by thyroid (227) and colorectal (183) cancers. Switch genes codifying for kinases were 14,7 and 3 respectively.We looked for three homology sequences identified across vemurafenib targets and we found that thyroid cancer and lung adenocarcinoma have a similar number of putative targetable switch genes kinase (5-6); on the contrary, colorectal cancer has just one,with minor homology sequence

Circulating miR-26b-5p and miR-451a as diagnostic biomarkers in medullary thyroid carcinoma patients

Purpose/methodsThe determination of tumour biomarkers is paramount to advancing personalized medicine, more so in rare tumours like medullary thyroid carcinoma (MTC), whose diagnosis is still challenging. The aim of this study was to identify non-invasive circulating biomarkers in MTC. To achieve this goal, paired MTC tissue and plasma extracellular vesicle samples were collected from multiple centres and microRNA (miRNA) expression levels were evaluated.ResultsThe samples from a discovery cohort of 23 MTC patients were analysed using miRNA arrays. Lasso logistic regression analysis resulted in the identification of a set of circulating miRNAs as diagnostic biomarkers. Among them, miR-26b-5p and miR-451a, were highly expressed and their expression decreased during follow-up in disease-free patients in the discovery cohort. Circulating miR-26b-5p and miR-451a were validated using droplet digital PCR in a second independent cohort of 12 MTC patients.ConclusionThis study allowed the identification and validation of a signature of two circulating miRNAs, miR-26b-5p and miR-451a, in two independent cohorts reporting a significant diagnostic performance for MTC. The results of this study offer advancements in molecular diagnosis of MTC proposing a novel non-invasive tool to use in precision medicine

Hypermethioninemia in Campania: Results from 10 years of newborn screening

In the last years tandem mass spectrometry (MS/MS) has become a leading technology used for neonatal screening purposes. Newborn screening by MS/MS on dried blood spot samples (DBS) has one of its items in methionine levels: the knowledge of this parameter allows the identification of infant affected by homocystinuria (cystathionine β-synthase, CBS, deficiency) but can also lead, as side effect, to identify cases of methionine adenosyltransferase (MAT) type I/III deficiency. We started an expanded newborn screening for inborn errors of metabolism in Campania region in 2007. Here we report our ten years experience on expanded newborn screening in identifying patients affected by hypermethioninemia. During this period we screened approximately 77,000 infants and identified two cases: one case of classical homocystinuria and one patient affected by defect of MAT I/III. In this paper we describe these patients and their biochemical follow-up and review the literature concerning worldwide newborn screening reports on incidence of CBS and MAT deficiency

FRP reinforcement in RC structures

fib Bulletin 40 deals mainly with the use of FRP bars as internal reinforcement for concrete structures. The background of the main physical and mechanical properties of FRP reinforcing bars is presented, with special emphasis on durability aspects. For each of the typical ultimate and serviceability limit states, the basic mechanical model is given, followed by different design models according to existing codes or design guidelines

Flexural performance of concrete beams reinforced with steel–FRP composite bars

YesFlexural performance of concrete beams reinforced with steel–FRP composite bar (SFCB) was investigated in this paper. Eight concrete beams reinforced with different bar types, namely one specimen reinforced with steel bars, one with fiber-reinforced polymer (FRP) bars and four with SFCBs, while the last two with hybrid FRP/steel bars, were tested to failure. Test results showed that SFCB/hybrid reinforced specimens exhibited improved stiffness, reduced crack width and larger bending capacity compared with FRP-reinforced specimen. According to compatibility of strains, materials’ constitutive relationships and equilibrium of forces, two balanced situations, three different failure modes and balanced reinforcement ratios as well as analytical technique for predicting the whole loading process are developed. Simplified formulas for effective moment of inertia and crack width are also proposed. The predicted results are closely correlated with the test results, confirming the validity of the proposed formulas for practical use.National Natural Science Foundation of China (51678514), China Postdoctoral Science Foundation (2018M642335), the Science and Technology Project of Jiangsu Construction System (2018ZD047), the Cooperative Education Project of Ministry of Education, China (201901273053), the Blue Project Youth Academic Leader of Colleges and Universities in Jiangsu Province (2020), the Six Talent Peaks Project of Jiangsu Province (JZ038, 2016) and the Yangzhou University Top Talents Support Projec

Assessment of performance of steel and GFRP bars as injected anchors in masonry walls

The first part of the paper presents the results of two series of in situ pull-out tests of injected anchors embedded in existing yellow tuff masonry walls. Anchors are made of steel and GFRP bars with diameter of 12 and 20 mm and were embedded by means of two types of grout: a cement-based and a pozzolana-based grout. The results were examined in terms of both maximum load and displacement to determine the most efficient bar-grout coupling. The experimental pull-out forces are also compared with the predictions given by several literature formulas. The second part of the paper is devoted to verify the efficiency of the tested injected anchor systems for avoiding out-of-plane damage mechanisms in masonry walls having varying slenderness and subjected to horizontal forces; to this aim parametric analyses were performed to calculate the seismic acceleration required to activate several out-of-plane mechanisms and to verify the effectiveness of injected anchors similar to those experimentally tested into avoiding them

In situ pull-out tests of steel and GFRP injected anchors in masonry walls

This paper presents the results of experimental in situ pull-out tests of injected anchors embedded in existing masonry walls made of yellow tuff. Anchors made of steel and GFRP bars of 20 mm in diameter were embedded in the masonry using two types of grout: a cement-based and a pozzolanabased grout. The experimental results were examined in terms of both maximum load and displacement to determine the most efficient bar-grout coupling. The experimental results were also compared with theoretical pull-out forces given by some standard code and literature strength models