Mean daily levels of urinary E1C (ng/mg creatinine; A) and PdG (μg/mg creatinine; B), by smoking status, in one representative cycle per participant

<p><b>Copyright information:</b></p><p>Taken from "Cigarette Smoking and Effects on Hormone Function in Premenopausal Women"</p><p>Environmental Health Perspectives 2005;113(10):1285-1290.</p><p>Published online 3 Jun 2005</p><p>PMCID:PMC1281267.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p> Cycles are centered on the estimated day of ovulation (labeled day 0), so negative days are in the follicular phase and positive days are in the luteal phase. Data from Women’s Reproductive Health Study, California Department of Health Services

Mean daily levels of urinary FSH (ng/mg creatinine) by smoking status during the luteal–follicular phase transition

<p><b>Copyright information:</b></p><p>Taken from "Cigarette Smoking and Effects on Hormone Function in Premenopausal Women"</p><p>Environmental Health Perspectives 2005;113(10):1285-1290.</p><p>Published online 3 Jun 2005</p><p>PMCID:PMC1281267.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p> Day 1 is the first bleed day of a cycle, or the last menstrual period (LMP); negative days are in the previous cycle. Smokers exclude one woman with very high FSH values (see “FSH findings”); with her included, differences would be greater and extend farther into days 7–12. Data from Women’s Reproductive Health Study, California Department of Health Services

Adopce a její význam v prevenci psychické deprivace

Sputum basophil gating strategy and change in marker expression. Representative sputum dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3Rι PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue), 7 h post-allergen (green) and 24 h post-allergen challenge (orange), compared to the isotype control (red). (TIFF 10198 kb

Additional file 6: Figure S6. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Basophil activation marker dose response to IL-25. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-25 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2740 kb

Additional file 5: Figure S5. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Basophil activation marker dose response to IL-33. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-33 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2756 kb

Additional file 1: Figure S1. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Bone marrow basophil gating strategy and change in marker expression. Representative bone marrow dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3Rι PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue) and 24 h post-allergen challenge (green), compared to the isotype control (red). (TIFF 10587 kb