Basophil activation marker dose response to IL-33. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-33 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2756 kb
