Sputum basophil gating strategy and change in marker expression. Representative sputum dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3RÎą PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue), 7Â h post-allergen (green) and 24Â h post-allergen challenge (orange), compared to the isotype control (red). (TIFF 10198 kb
