Polymorphism of Eimerian Oocysts: A Dilemma Posed by Working with Some Naturally Infected Hosts

Morphological variation of sporulated oocysts within individual eimerian species is well documented (Joyner, 1982. In Biology of the Coccidia, P. L. Long (ed.). University Park Press, Baltimore, pp. 35-62). In some cases, oocysts of a single eimerian species are known to vary in size by as much as 40% during patency (Duszynski, 1971, Journal of Parasitology 57: 948-952). During a survey to determine the prevalence of coccidiosis in sandhill cranes (Grus canadensis) wintering in New Mexico (Parker and Duszynski, 1986, Journal of Wildlife Diseases 21: 25-35), marked polymorphism was observed among sporulated oocysts of Eimeria reichenowi. Oocysts were obtained from intestinal contents of cranes necropsied in the field and processed for study by routine sporulation and flotation techniques (Duszynski et al., 1982, Journal of Parasitology 68: 1,146-1,148). Initially, oocysts were categorized into 5 groups based on obvious qualitative/quantitative features including oocyst wall texture, appearance of the sporocyst residuum, and the number of polar bodies. Fifty-seven of 118 (48%) fecal samples with E. reichenowi contained 2 or more morphological types of oocysts. Oocysts were measured under oil immersion (100 x Neofluar objective, Zeiss Universal Photomicroscope) and differences between the 5 oocyst groups were tested for significance (P \u3c0.05) using the Student-Newman-Keuls procedure

Energy and Nutrient Recovery fromCattle Feedlots

Selective harvesting of manure can benefit cattle producers by creating a product of value. A tool that identifies locations of manure accumulation has been developed using a subsurface sensor (Dualem-1S, Milton, ON) and software designed for salt mapping (ESAP, Riverside, CA). The combination allowed the development of models to estimate higher heating value (HHV) of feedlot manure across a feedlot pen. Soil sample data from cattle feedlots in Texas and Nebraska were analyzed for volatile solids (VSs) then combined with the Dualem-1S apparent soil conductivity (ECa) data to produce models having correlations between associated ECa values and VS (r2 = 0.869, VS). A corresponding model is under development to estimate the moisture content of the collectable solids. The combined models allow real-time spatial estimates of HHV within a feedlot pen. These methods will allow direct harvesting of VS for use as a recoverable energy source through direct combustion or cocombustion

Energy and Nutrient Recovery fromCattle Feedlots

Selective harvesting of manure can benefit cattle producers by creating a product of value. A tool that identifies locations of manure accumulation has been developed using a subsurface sensor (Dualem-1S, Milton, ON) and software designed for salt mapping (ESAP, Riverside, CA). The combination allowed the development of models to estimate higher heating value (HHV) of feedlot manure across a feedlot pen. Soil sample data from cattle feedlots in Texas and Nebraska were analyzed for volatile solids (VSs) then combined with the Dualem-1S apparent soil conductivity (ECa) data to produce models having correlations between associated ECa values and VS (r2 = 0.869, VS). A corresponding model is under development to estimate the moisture content of the collectable solids. The combined models allow real-time spatial estimates of HHV within a feedlot pen. These methods will allow direct harvesting of VS for use as a recoverable energy source through direct combustion or cocombustion

Manure Harvesting Frequency: The Key to Feedyard Dust Control in a Summer Drought

4 pp., 4 photosSummer drought can make dust control in feedyards more challenging than usual. Supplemental water may not keep pace with daily evaporation. The key to dust control is managing the depth of dry manure in the pens by harvesting manure more frequently, and planning ahead when a drought is projected

AAPM medical physics practice guideline 10.a.: Scope of practice for clinical medical physics.

The American Association of Physicists in Medicine (AAPM) is a nonprofit professional society whose primary purposes are to advance the science, education, and professional practice of medical physics. The AAPM has more than 8000 members and is the principal organization of medical physicists in the United States. The AAPM will periodically define new practice guidelines for medical physics practice to help advance the science of medical physics and to improve the quality of service to patients throughout the United States. Existing medical physics practice guidelines will be reviewed for the purpose of revision or renewal, as appropriate, on their fifth anniversary or sooner. Each medical physics practice guideline (MPPG) represents a policy statement by the AAPM, has undergone a thorough consensus process in which it has been subjected to extensive review, and requires the approval of the Professional Council. The medical physics practice guidelines recognize that the safe and effective use of diagnostic and therapeutic radiation requires specific training, skills, and techniques as described in each document. As the review of the previous version of AAPM Professional Policy (PP)-17 (Scope of Practice) progressed, the writing group focused on one of the main goals: to have this document accepted by regulatory and accrediting bodies. After much discussion, it was decided that this goal would be better served through a MPPG. To further advance this goal, the text was updated to reflect the rationale and processes by which the activities in the scope of practice were identified and categorized. Lastly, the AAPM Professional Council believes that this document has benefitted from public comment which is part of the MPPG process but not the AAPM Professional Policy approval process. The following terms are used in the AAPM's MPPGs: Must and Must Not: Used to indicate that adherence to the recommendation is considered necessary to conform to this practice guideline. Should and Should Not: Used to indicate a prudent practice to which exceptions may occasionally be made in appropriate circumstances

Inhibition of angiogenesis and suppression of colorectal cancer metastatic to the liver using the Sleeping Beauty Transposon System

<p>Abstract</p> <p>Background</p> <p>Metastatic colon cancer is one of the leading causes of cancer-related death worldwide, with disease progression and metastatic spread being closely associated with angiogenesis. We investigated whether an antiangiogenic gene transfer approach using the <it>Sleeping Beauty </it>(SB) transposon system could be used to inhibit growth of colorectal tumors metastatic to the liver.</p> <p>Results</p> <p>Liver CT26 tumor-bearing mice were hydrodynamically injected with different doses of a plasmid containing a transposon encoding an angiostatin-endostatin fusion gene (Statin AE) along with varying amounts of SB transposase-encoding plasmid. Animals that were injected with a low dose (10 μg) of Statin AE transposon plasmid showed a significant decrease in tumor formation only when co-injected with SB transposase-encoding plasmid, while for animals injected with a higher dose (25 μg) of Statin AE transposon, co-injection of SB transposase-encoding plasmid did not significantly affect tumor load. For animals injected with 10 μg Statin AE transposon plasmid, the number of tumor nodules was inversely proportional to the amount of co-injected SB plasmid. Suppression of metastases was further evident in histological analyses, in which untreated animals showed higher levels of tumor cell proliferation and tumor vascularization than animals treated with low dose transposon plasmid.</p> <p>Conclusion</p> <p>These results demonstrate that hepatic colorectal metastases can be reduced using antiangiogenic transposons, and provide evidence for the importance of the transposition process in mediating suppression of these tumors.</p

Spitzer IRAC observations of newly-discovered planetary nebulae from the Macquarie-AAO-Strasbourg H-alpha Planetary Nebula Project

We compare H-alpha, radio continuum, and Spitzer Space Telescope (SST) images of 58 planetary nebulae (PNe) recently discovered by the Macquarie-AAO-Strasbo- urg H-alpha PN Project (MASH) of the SuperCOSMOS H-alpha Survey. Using InfraRed Array Camera (IRAC) data we define the IR colors of PNe and demonstrate good isolation between these colors and those of many other types of astronomical object. The only substantive contamination of PNe in the color-color plane we illustrate is due to YSOs. However, this ambiguity is readily resolved by the unique optical characteristics of PNe and their environs. We also examine the relationships between optical and MIR morphologies from 3.6 to 8.0um and explore the ratio of mid-infrared (MIR) to radio nebular fluxes, which is a valuable discriminant between thermal and nonthermal emission. MASH emphasizes late evolutionary stages of PNe compared with previous catalogs, enabling study of the changes in MIR and radio flux that attend the aging process. Spatially integrated MIR energy distributions were constructed for all MASH PNe observed by the GLIMPSE Legacy Project, using the H-alpha morphologies to establish the dimensions for the calculations of the Midcourse Space Experiment (MSX), IRAC, and radio continuum (from the Molonglo Observatory Synthesis Telescope and the Very Large Array) flux densities. The ratio of IRAC 8.0-um to MSX 8.3-um flux densities provides a measure of the absolute diffuse calibration of IRAC at 8.0 um. We independently confirm the aperture correction factor to be applied to IRAC at 8.0um to align it with the diffuse calibration of MSX. The result agrees with the recommendations of the Spitzer Science Center and with results from a parallel study of HII regions. These PNe probe the diffuse calibration of IRAC on a spatial scale of 9-77 arcsec.Comment: 48 pages, LaTeX (aastex), incl. 18 PostScript (eps) figures and 3 tables. Accepted by Astrophysical Journa

Ensembl 2011

The Ensembl project (http://www.ensembl.org) seeks to enable genomic science by providing high quality, integrated annotation on chordate and selected eukaryotic genomes within a consistent and accessible infrastructure. All supported species include comprehensive, evidence-based gene annotations and a selected set of genomes includes additional data focused on variation, comparative, evolutionary, functional and regulatory annotation. The most advanced resources are provided for key species including human, mouse, rat and zebrafish reflecting the popularity and importance of these species in biomedical research. As of Ensembl release 59 (August 2010), 56 species are supported of which 5 have been added in the past year. Since our previous report, we have substantially improved the presentation and integration of both data of disease relevance and the regulatory state of different cell types

Combating subclonal evolution of resistant cancer phenotypes

Metastatic breast cancer remains challenging to treat, and most patients ultimately progress on therapy. This acquired drug resistance is largely due to drug-refractory sub-populations (subclones) within heterogeneous tumors. Here, we track the genetic and phenotypic subclonal evolution of four breast cancers through years of treatment to better understand how breast cancers become drug-resistant. Recurrently appearing post-chemotherapy mutations are rare. However, bulk and single-cell RNA sequencing reveal acquisition of malignant phenotypes after treatment, including enhanced mesenchymal and growth factor signaling, which may promote drug resistance, and decreased antigen presentation and TNF-α signaling, which may enable immune system avoidance. Some of these phenotypes pre-exist in pre-treatment subclones that become dominant after chemotherapy, indicating selection for resistance phenotypes. Post-chemotherapy cancer cells are effectively treated with drugs targeting acquired phenotypes. These findings highlight cancer's ability to evolve phenotypically and suggest a phenotype-targeted treatment strategy that adapts to cancer as it evolves