Influence of the brood rearing temperature on honey bee development and susceptibility to intoxication by pesticides

contribution to session IV Test methodolog

Endobronchial solitary fibrous tumor

Solitary fibrous tumor (SFT) is a mesenchymal neoplasm that appears primarily in the pleura and rarely in intrapulmonary or endobronchial topography. The authors report the case of a 47-year-old woman who presented obstructive respiratory symptoms for 4 years. The chest computed tomography and bronchoscopy showed an obstructive polypoid lesion located between the trachea and the left main bronchus associated with distal atelectasis of the left lung. A resection of the lesion was performed and, macroscopically, the mass was oval, encapsulated, and firm, measuring 2.3 × 1.7 × 1.5 cm. Histology revealed low-grade mesenchymal spindle cell neoplasm, with alternating cellularity, myxoid areas, and mature adipose tissue outbreaks, as well as blood vessels with irregular walls. The immunohistochemical study was positive for CD34, CD99, and BCL2. The diagnosis was SFT in an unusual topography. The patient’s symptoms remitted after tumor excision, and no systemic problems were evident. SFTs primarily affect adults and often follow a benign course; however, their behavior is unpredictable. The presence of necrosis and mitotic activity may portend a poor prognosis. Endobronchial SFTs are rare but should be evaluated and monitored similar to SFTs at other sites, with a long-term follow-u

The Grizzly, April 12, 2007

Project Pericles Continues to Succeed in Community Service • Macs Lag in Environmental Friendliness According to Greenpeace • New Dorm Progress • Ursinus Freshmen Fall Behind in Collegiate Learning Assessment • Spread Your Legs and Turn Your Head and Cough • Relay for Life Fast Approaching • Ursinus, Break the Silence! • Nutrition Tips: Veggie Report • Opinions: Dear Mahmoud • Bears Terrorize McDaniel, Sit Atop CC Standings • Bears Finish Second at NCGA Championshipshttps://digitalcommons.ursinus.edu/grizzlynews/1737/thumbnail.jp

Honey bee brood ring-test: method for testing pesticide toxicity on honeybee brood in laboratory conditions

contribution to session IV Test methodology The Experimental unit of entomology (INRA, France) developed a new in vitro method to assess effects of pesticides on honey bee larvae. The method consists in rearing bee larvae in plastic cells. The larvae are fed with diet containing 50% of fresh royal jelly and 50% of an aqueous sugar and yeast extract solution, and reared in an incubator at 35 °C and 96% relative humidity. According to that method, 9 tests (7 in 2008 and 2 in 2005) were carried out in 7 laboratories and different countries. The objective of these trials was to assess the LD50 for dimethoate 48 hours after an acute exposure. The LD50 values ranged from 1.5 μg a.i./larva to 8.8 μg a.i./larva, with 2 tests with particularly high values (5.0 and 8.8 μg a.i./larva). In 7 tests, these values ranged from 1.5 μg a.i./larva to 3.1 μg a.i./larva. Such variability may be due to the colony origin, the season and larva heterogeneity at grafting. Solutions are proposed to improve the method through the continuation of the ring test. Keywords: Apis mellifera, brood, in vitro test, dimethoat

Endobronchial solitary fibrous tumor

Solitary fibrous tumor (SFT) is a mesenchymal neoplasm that appears primarily in the pleura and rarely in intrapulmonary or endobronchial topography. The authors report the case of a 47-year-old woman who presented obstructive respiratory symptoms for 4 years. The chest computed tomography and bronchoscopy showed an obstructive polypoid lesion located between the trachea and the left main bronchus associated with distal atelectasis of the left lung. A resection of the lesion was performed and, macroscopically, the mass was oval, encapsulated, and firm, measuring 2.3 × 1.7 × 1.5 cm. Histology revealed low-grade mesenchymal spindle cell neoplasm, with alternating cellularity, myxoid areas, and mature adipose tissue outbreaks, as well as blood vessels with irregular walls. The immunohistochemical study was positive for CD34, CD99, and BCL2. The diagnosis was SFT in an unusual topography. The patient’s symptoms remitted after tumor excision, and no systemic problems were evident. SFTs primarily affect adults and often follow a benign course; however, their behavior is unpredictable. The presence of necrosis and mitotic activity may portend a poor prognosis. Endobronchial SFTs are rare but should be evaluated and monitored similar to SFTs at other sites, with a long-term follow-u

A Teatro con INAF - Il Portfolio degli spettacoli dell’Istituto Nazionale di Astrofisica

A dicembre 2020, il Gruppo Storie dell’Istituto Nazionale di Astrofisica (INAF) ha pubblicato insieme al magazine di Didattica e Divulgazione dell’Ente, EduINAF, il Portfolio degli spettacoli dell’INAF. Il Portfolio è il risultato di un’accurata ricognizione in collaborazione con i referenti degli stessi ed è unico nel suo genere per un Ente di ricerca. Comprende una trentina di spettacoli, a tema astrofisico o scientifico, prodotti negli anni dal personale INAF in autonomia o in collabora-zione con realtà professionali. Nel Portfolio, ogni spettacolo è descritto in una scheda informativa e la fruizione è resa immediata grazie a filtri appositi. Il Portfolio è man mano aggiornato per meglio rispondere alle esigenze e curiosità del pubblico in cerca di approcci non tradizionali nell’esplorazione dell’astronomia e della ricerca in generale. Ad accompagnare il Portfolio, un breve video per la disseminazione sui canali social e un segnalibro dedicato, in preparazione, da offrire in incontri con scuole e in eventi con il pubblico. Grazie alla visione d’insieme offerta dal Portfolio, nell’Ente sono nate valutazioni per massimizzare la visibilità degli spettacoli tramite l’organizzazione di un ‘Festival INAF diffuso’. In questo report descriviamo il Portfolio, il suo contesto, le criticità riscontrate e i progetti futuri

Influence of brood rearing temperature on honey bee development and susceptibility to poisoning by pesticides

Adult honey bees (Apis mellifera) usually maintain colony brood rearing temperature between 34-35\ub0C by thermoregulation. The brood may, however, also be subjected to suboptimal temperature. Here weinvestigated whether a decrease of brood rearing temperature mayhave effects on larval mortality, adult emergence, longevity, morphology and susceptibility to poisoning by pesticides (dimethoate).Using the in vitrorearing protocol of Aupinel (2005), we were able for the first time to control the brood temperature not only during the pupalstage but also during the larval stage. Honey bee larvae were reared in vitroat 35\ub0C (optimal) and 33\ub0C (suboptimal) from 12 h after hatching for 15 days. Dimethoate was tested by ingestion either on 4-day old larvae or on 7-day old adults. Our results showed that lower rearing temperature had no significant effects on larval mortality and adult emergence, but adult bee mortality was strongly affected. Moreover, adult workers emerging at 33\ub0C were significantly more susceptible to dimethoate. Larval LD50 (48 h) was, however, 28 times higher at33\ub0C than at 35\ub0C. The striking differences between larvae and adults may beexplained by differential larval metabolism at 33\ub0C and resulting slower active ingredient absorption. We conclude that adult honey bees reared at even slightly suboptimal brood temperature may be more susceptible to pesticide poisoning and be characterised by reduced longevity. Thus, low temperature brood rearing could be another stress factor for colonies

Estudo do destino dos cães no canil municipal de Botucatu antes e após a Lei nº 12.916 que dispõe sobre o controle da reprodução de cães

Despite the gathering of dogs on public streets, homes and by the Centers for Zoonoses Control is a practice adopted that does not control the dog population, it is an activity considered as primary and supplementary of the zoonoses control. The canine surplus population, besides the important role in the chain of transmission of various diseases, is also due to problems of aggressions, traffic accidents and environmental impacts. Considering the lack of information on the dynamics destination of dogs in kennels after the State Law number 12.916/08 (provides for the control of dog reproduction and gives related provisions), the present study aimed to analyze the destination of dogs gathered by the Municipality Botucatu and transported to the Municipal Kennel and also donated by the population comparison before and after the implementation of the Law. Were analyzed 4038 input records of the dogs of the kennel, which were stored in the database generated by EXCEL spreadsheet and analyzed through Census. Before the Law, were euthanized 2193 (70%) and after 639 dogs (69%), percentage terms values are similar, however, there is a difference of 1557 dogs euthanized. This difference can be explained by the veterinary medical care offered to dogs gathered after the Law, for subsequent adoption. Since before the only measure taken was euthanasia. The results concluded that the law imposes considerable changes in behaviors and procedures performed in the kennel, especially the decrease in the number of euthanized dogs and the adoption of more selective criteria for the gathering of dogs on public roads.A pesar de la recogida de perros en las calles, domicilios y la acogida por el Centro de Control de Zoonosis no ser una práctica adoptada para controlar la población de perros, es una actividad que se considera medida primaria y complementaria para controlar las zoonosis. El exceso de población canina, y su importancia en la cadena transmisión de diversas enfermedades también representa problemas como consecuencia de las agresiones, accidentes de tránsito e impactos ambientales. Teniendo en cuenta la falta de información sobre la dinámica del destino de los perros en las perreras después de la Ley del Estado número 12.916/08 (que dispone sobre control de reproducción canina y disposiciones conexas), el presente estudio tuvo como objetivo analizar el destino de los perros de la Intendencia Municipal de Botucatu y transportados a la perrera Municipal, y también los donados por la población en comparación anterior y posterior a la implementación de la ley. Fueron analizadas 4038 fichas de entrada de perros en la perrera, las cuales se almacenaron en la base de datos generada en EXCEL y analizadas mediante Censo. Antes de la ley, fueron sacrificados 2193 (70%) perros y después de la ley, 639 (69%); los valores porcentuales son similares, sin embargo, hay una diferencia de 1.557 perros eutanasiados. Esta diferencia se explica por la atención médica veterinaria que se ofrece a los perros recogidos después de la ley para su posterior adopción, una vez que antes la única medida tomada era la eutanasia. Los resultados concluyeron que la ley impuso cambios en los procedimientos en la perrera, principalmente para reducir el número de perros eutanasiados, como consecuencia, se incrementó la permanencia de estos animales. También las razones predominantes para la recogida de los perros de las calles son los que tenían algún tipo de riesgo para la población.Apesar do recolhimento de cães em via pública, domicílios ou do acolhimento pelos Centros de Controle de Zoonoses ser uma prática adotada que não controla a população canina, é uma atividade considerada medida primária e complementar de controle de zoonoses. O excedente populacional canino, além de sua importância na cadeia de transmissão de diversas doenças, representa também problemas em decorrência das agressões, acidentes de trânsito e impactos ambientais. Considerando a falta de informações sobre a dinâmica do destino de cães em canis após a Lei Estadual número 12.916/08 (dispõe sobre o controle da reprodução de cães e dá providências correlatas), o presente trabalho objetivou analisar o destino dos cães recolhidos pela Prefeitura Municipal de Botucatu e transportados ao Canil Municipal e também os doados pela população, comparativamente antes e depois da implementação da Lei. Foram analisadas 4038 fichas de entrada de cães do Canil, as quais foram armazenadas no banco de dados gerados pela planilha EXCEL e analisados por meio de Censo. Antes da Lei, eram eutanasiados 2193 (70%) cães e após, 639 (69%), percentualmente os valores são similares, entretanto, há uma diferença de 1557 cães eutanasiados. Esta diferença pode ser explicada pelos cuidados médicos veterinários oferecidos aos cães recolhidos, após a Lei, para posterior adoção, uma vez que antes a única medida adotada era a eutanásia. Os resultados permitiram concluir que a Lei impôs mudanças nos procedimentos no Canil, principalmente a diminuição do número de eutanásias realizadas, consequentemente aumentou a permanência desses animais. Também os motivos predominantes para o recolhimento dos cães

Leveraging process mining for modeling progression trajectories in amyotrophic lateral sclerosis

Abstract Background Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease whose spreading and progression mechanisms are still unclear. The ability to predict ALS prognosis would improve the patients’ quality of life and support clinicians in planning treatments. In this paper, we investigate ALS evolution trajectories using Process Mining (PM) techniques enriched to both easily mine processes and automatically reveal how the pathways differentiate according to patients’ characteristics. Methods We consider data collected in two distinct data sources, namely the Pooled Resource Open-Access ALS Clinical Trials (PRO-ACT) dataset and a real-world clinical register (ALS–BS) including data of patients followed up in two tertiary clinical centers of Brescia (Italy). With a focus on the functional abilities progressively impaired as the disease progresses, we use two Process Discovery methods, namely the Directly-Follows Graph and the CareFlow Miner, to mine the population disease trajectories on the PRO-ACT dataset. We characterize the impairment trajectories in terms of patterns, timing, and probabilities, and investigate the effect of some patients’ characteristics at onset on the followed paths. Finally, we perform a comparative study of the impairment trajectories mined in PRO-ACT versus ALS–BS. Results We delineate the progression pathways on PRO-ACT, identifying the predominant disabilities at different stages of the disease: for instance, 85% of patients enter the trials without disabilities, and 48% of them experience the impairment of Walking/Self-care abilities first. We then test how a spinal onset increases the risk of experiencing the loss of Walking/Self-care ability as first impairment (52% vs. 27% of patients develop it as the first impairment in the spinal vs. the bulbar cohorts, respectively), as well as how an older age at onset corresponds to a more rapid progression to death. When compared, the PRO-ACT and the ALS–BS patient populations present some similarities in terms of natural progression of the disease, as well as some differences in terms of observed trajectories plausibly due to the trial scheduling and recruitment criteria. Conclusions We exploited PM to provide an overview of the evolution scenarios of an ALS trial population and to preliminary compare it to the progression observed in a clinical cohort. Future work will focus on further improving the understanding of the disease progression mechanisms, by including additional real-world subjects as well as by extending the set of events considered in the impairment trajectories

Leveraging Process Mining for Modeling Progression Trajectories in Amyotrophic Lateral Sclerosis

Abstract Background Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease whose spreading and progression mechanisms are still unclear. The ability to predict ALS prognosis would improve the patients’ quality of life and support clinicians in planning treatments. In this paper, we investigate ALS evolution trajectories using Process Mining (PM) techniques enriched to both easily mine processes and automatically reveal how the pathways differentiate according to patients’ characteristics. Methods We consider data collected in two distinct data sources, namely the Pooled Resource Open-Access ALS Clinical Trials (PRO-ACT) dataset and a real-world clinical register (ALS–BS) including data of patients followed up in two tertiary clinical centers of Brescia (Italy). With a focus on the functional abilities progressively impaired as the disease progresses, we use two Process Discovery methods, namely the Directly-Follows Graph and the CareFlow Miner, to mine the population disease trajectories on the PRO-ACT dataset. We characterize the impairment trajectories in terms of patterns, timing, and probabilities, and investigate the effect of some patients’ characteristics at onset on the followed paths. Finally, we perform a comparative study of the impairment trajectories mined in PRO-ACT versus ALS–BS. Results We delineate the progression pathways on PRO-ACT, identifying the predominant disabilities at different stages of the disease: for instance, 85% of patients enter the trials without disabilities, and 48% of them experience the impairment of Walking/Self-care abilities first. We then test how a spinal onset increases the risk of experiencing the loss of Walking/Self-care ability as first impairment (52% vs. 27% of patients develop it as the first impairment in the spinal vs. the bulbar cohorts, respectively), as well as how an older age at onset corresponds to a more rapid progression to death. When compared, the PRO-ACT and the ALS–BS patient populations present some similarities in terms of natural progression of the disease, as well as some differences in terms of observed trajectories plausibly due to the trial scheduling and recruitment criteria. Conclusions We exploited PM to provide an overview of the evolution scenarios of an ALS trial population and to preliminary compare it to the progression observed in a clinical cohort. Future work will focus on further improving the understanding of the disease progression mechanisms, by including additional real-world subjects as well as by extending the set of events considered in the impairment trajectories