MOESM3 of Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation

Additional file 3. Summary of lesion and PCR detection and quantification of N. caninum in placenta and foetal liver and brain. dpi: days post infection when abortion occurred; ASF: Average size of focus; %LES: Percentage of section affected by lesions. * All lambs from this group gave birth to viable lambs between days 142 and 155 of gestation. Three of nine lambs were born prematurely prior to day 145, and exhibited weakness, recumbency and unresponsiveness to external stimuli. 1 Percentage of the section affected by lesions. 2 Fractions represent the number of positive animals/total number of animals checked by nested-ITS1 PCR, and figures within brackets represent the median values of parasite burden (tachyzoites/mg tissue). a, b Values followed by unlike superscripts differ significantly by Dunn’s test for pairwise comparisons. c, d Fractions determined for positive animals followed by unlike superscripts differ significantly by Fisher’s exact test

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Not AvailableFive classical clustering method s: four hierarchical-single linkage, average-between linkage, average-within linkage, Wards-and one non-hi era rchical-k-means-using five different distance measures: squared Euclidean, city block, Chebychev's, Pearson correlation and Minkowski have been compared on the basis of simu lated multivariate data on paddy crop genotypes. The performance of different clustering methods was compared based on the average percentage probability of misclassification an its standard error. The performance of different hierarchical clustering methods varied with distance measures used and it was found that squared Euclidean performed best among the five distances followed by city block distance in majority of cases. Among the five methods, the Ward's method performed best with least average percentage probability of misdassification followed by non-hierarchical k-means method irrespective of the sample size. Among the different distance measures used under hierarchical clustering methods, the squared Euclidean distance showed least average percentage probability of misclassification followed by city block distance.Not Availabl

MOESM4 of Experimental ovine toxoplasmosis: influence of the gestational stage on the clinical course, lesion development and parasite distribution

Additional file 4 Individual frequency of parasite DNA detection in infected animals. Table showing the individual frequency of parasite DNA detection in infected animals

MOESM3 of Experimental ovine toxoplasmosis: influence of the gestational stage on the clinical course, lesion development and parasite distribution

Additional file 3 Box-plot graph T. gondii burdens measured at the same period post infection and comparing between the three groups at the placenta and foetal viscera. Box-plot graphs represent the median burden, the lower and upper quartiles (boxes) and minimum and maximum values (whiskers). (*) indicates P < 0.05 significant differences between groups in each period post infection