Toolbox for Non-Intrusive Structural and Functional Analysis of Recombinant VLP Based Vaccines: A Case Study with Hepatitis B Vaccine

Background: Fundamental to vaccine development, manufacturing consistency, and product stability is an understanding of the vaccine structure-activity relationship. With the virus-like particle (VLP) approach for recombinant vaccines gaining popularity, there is growing demand for tools that define their key characteristics. We assessed a suite of non-intrusive VLP epitope structure and function characterization tools by application to the Hepatitis B surface antigen (rHBsAg) VLP-based vaccine. Methodology: The epitope-specific immune reactivity of rHBsAg epitopes to a given monoclonal antibody was monitored by surface plasmon resonance (SPR) and quantitatively analyzed on rHBsAg VLPs in-solution or bound to adjuvant with a competitive enzyme-linked immunosorbent assay (ELISA). The structure of recombinant rHBsAg particles was examined by cryo transmission electron microscopy (cryoTEM) and in-solution atomic force microscopy (AFM). Principal Findings: SPR and competitive ELISA determined relative antigenicity in solution, in real time, with rapid turnaround, and without the need of dissolving the particulate aluminum based adjuvant. These methods demonstrated the nature of the clinically relevant epitopes of HBsAg as being responsive to heat and/or redox treatment. In-solution AFM and cryoTEM determined vaccine particle size distribution, shape, and morphology. Redox-treated rHBsAg enabled 3D reconstruction from CryoTEM images – confirming the previously proposed octahedral structure and the established lipidto-protei

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three genomic nomenclature systems to all sequence data from the World Health Organization European Region available until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation, compare the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2

Zmiany klimatyczne a funkcjonowanie ośrodków narciarskich – studium przypadku stacji Podstolice-SKI

Cechą współczesnej turystyki jest przejście od typu pasywnego do aktywnego spędzania czasu wolnego, w tym różnorakich aktywności sportowych. W związku z tym wiele miejscowości posiadających odpowiednie walory przyrodnicze realizuje usługi rekreacyjno-sportowe. Narciarstwo zjazdowe i snowboarding to dyscypliny aktywności ruchowej, które w Polsce mają dużą popularność. Na przestrzeni ostatnich lat obserwuje się bardzo dynamiczny rozwój nowych ośrodków narciarskich, jak i duże inwestycje w obrębie ośrodków już istniejących. Na przykładzie ośrodka Podstolice-SKI, zlokalizowanego w obrębie wschodniej części Pogórza Wielickiego, mającego w części zaspokoić zapotrzebowanie na usługi narciarskie mieszkańców aglomeracji krakowskiej, autorzy podjęli próbę analizy bieżącej działalności ośrodka oraz w oparciu o dane klimatologiczne, uzyskane z reprezentatywnych stacji pomiarowych IMGW-PIB, analizowali jego rozwój w aspekcie zachodzących zmian klimatycznych

Impact of Meteorological Conditions on the Dynamics of the COVID-19 Pandemic in Poland

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by the novel coronavirus. The role of environmental factors in COVID-19 transmission is unclear. This study aimed to analyze the correlation between meteorological conditions (temperature, relative humidity, sunshine duration, wind speed) and dynamics of the COVID-19 pandemic in Poland. Data on a daily number of laboratory-confirmed COVID-19 cases and the number of COVID-19-related deaths were gatheredfrom the official governmental website. Meteorological observations from 55 synoptic stations in Poland were used. Moreover, reports on the movement of people across different categories of places were collected. A cross-correlation function, principal component analysis and random forest were applied. Maximum temperature, sunshine duration, relative humidity and variability of mean daily temperature affected the dynamics of the COVID-19 pandemic. An increase intemperature and sunshine hours decreased the number of confirmed COVID-19 cases. The occurrence of high humidity caused an increase in the number of COVID-19 cases 14 days later. Decreased sunshine duration and increased air humidity had a negative impact on the number of COVID-19-related deaths. Our study provides information that may be used by policymakers to support the decision-making process in nonpharmaceutical interventions against COVID-19

Climate of the Carpathian Region in the period 1961–2010: climatologies and trends of 10 variables

The Carpathians are the largest mountain range in Europe and they represent a geographic barrier between Central Europe, Eastern Europe, and the Balkans. In order to investigate the climate of the area, the CARPATCLIM project members compiled the Climate Atlas of the Carpathian Region, which consists of high-resolution daily grids (0.1˚ x 0.1˚) of sixteen meteorological variables and many derived indicators related to 1961-2010. We computed the gridded climatologies for 1961-2010 for eight variables (air pressure, cloudiness, precipitation, relative humidity, minimum and maximum temperature, sunshine duration, and wind speed) and we discuss their spatial patterns. For each variable, we calculated the gridded linear trends related to 1961-2010 both on annual and seasonal basis. In general, temperature was found to increase in every season in 1986-2010, confirming the trends occurring in Europe in the last decades. On the other way, wind speed decreased in every season. Cloudiness and relative humidity decreased in spring, summer, and winter, and increased in autumn, whilst sunshine duration, as expected, behaved in the opposite way. Precipitation slightly increased and air pressure showed no significant trend, except of a few grid points. Then, we dealt with the correlation between the variables: excluding the high elevation points, the most correlated are sunshine duration and temperature. In particular, positive and negative sunshine duration anomalies are found to be respectively correlated with positive and negative temperature anomalies during the global dimming (60’s and 70’s) and brightening (90’s and 2000’s) periods.JRC.H.7-Climate Risk Managemen